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Question: Question: In sequence read archive(SRA) database, is there any option to fill the MD5 checksum box. What i have to write there??

minakshi
3906 days ago

Question: In sequence read archive(SRA) database, is there any option to fill the MD5 checksum box. What i have to write there??

Actually i m working on Transcriptome shotgun assembly database. In TSA db, 3 submissions are there. I have done 2 i.e Bioproject and Biosample data submission. I m confusing about SRA database submission.

minakshi 3900 days ago

hello, Please suggest me .

Please enter file names, md5sums etc, then put files on ftp://sra:03o4!srRy!@ftp-private.ncbi.nih.gov/

you can use Windows Explorer but not Internet Explorer for uploads

From unix/linux:

Address: ftp-private.ncbi.nih.gov
Login: sra
Password: *******

Thank you.

minakshi 3899 days ago

Answers
0

I did't understand your question completly. I guess you are working with SRA database submission and need to know how to calculate MD5 checkhum.

Check out this link https://gist.github.com/audy/1251153 , hope helpful in your case.

More tutorial http://www.ncbi.nlm.nih.gov/books/NBK47529/

SRA toolkit : https://github.com/NCBITools/sratoolkit

0

jitendra@jitendra-OptiPlex-390[EBAv1.1] ftp ftp-private.ncbi.nlm.nih.gov                                                                          [12:20PM]
Connected to ftp-private.ncbi.nlm.nih.gov.
220-
 Warning Notice!
 
 You are accessing a U.S. Government information system which includes this
 computer, network, and all attached devices. This system is for
 Government-authorized use only. Unauthorized use of this system may result in
 disciplinary action and civil and criminal penalties. System users have no
 expectation of privacy regarding any communications or data processed by this
 system. At any time, the government may monitor, record, or seize any
 communication or data transiting or stored on this information system.
 ---
 Welcome to the NCBI ftp server! The anonymous access URL is ftp://ftp.ncbi.nlm.nih.gov/
 
 Public data may be downloaded by logging in as "anonymous" using your E-mail address as a password.
 
 Please see ftp://ftp.ncbi.nlm.nih.gov/README.ftp for hints on large file transfers
220 FTP Server ready.
Name (ftp-private.ncbi.nlm.nih.gov:jitendra): sra
331 Password required for sra
Password:
230 User sra logged in
Remote system type is UNIX.
Using binary mode to transfer files.
ftp>

0

Once you are on ftp command line. You can transfer your data and other stuff onto the server. 

For more ftp comman line detail visit http://www.tldp.org/HOWTO/FTP-3.html