Question: Question: In search of a good genome browser/viewer

anushreenarjala@rocketmail.com
3164 days ago

Question: In search of a good genome browser/viewer

I have reference sequeces (in fasta format) and alignment files (small RNA reads from 4 different conditions aligned to genome in SAM/BAM/BED/BEDgraph format) as input files. I'm interested in comaparing the alignments of different conditions. So, I'm looking for a good genome viewer which can also give you a publication quality visualization  of these four tracks. I have tried popular genome browsers like IGV, IGB, tview and many more. I'm not satisfied with any of these softwares. It would be nice if I can visualize each read only once, but somehow (either by width, heatmap or numbers beside them) highliting is abundance/depth at each position. So, is there any other better viewers or packages in R or Python which I can customise to my need? Thank you in advance.

Thank you everyone for your valuable answers. Finally, I had to agree with Rahul that currently there is no better genome browser than IGV. Still it has some limitations in visualizing small RNA data. 

anushreenarjala@rocketmail.com 3074 days ago

Best Answer
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Hi Anushree,

I am sorry, but your question confuses me. You have already tried the best genome browsers like IGV (as per my working experience). I guess, you can achieve all as IGV displays only one coverage track per alignment with preference and also highlight potential genetic alterations in reads.

Anyway, there are some few more options available in R that, I guess, could be useful:

R genome browser (Rgb): This package is dedicated to memory efficient genome browsing strategies. It includes classes and functions to manipulate genomic tracks, and a higher level interactive genome browser. More at http://bioinformatics.ovsa.fr/Rgb

Apart from that, R package "ggbio" could be useful as well for extending the grammar of graphics for genomic data. More at http://www.bioconductor.org/packages/2.11/bioc/html/ggbio.html

The rtracklayer package is an interface (or layer ) between R and genome browsers. More at http://svitsrv25.epfl.ch/R-doc/library/rtracklayer/doc/rtracklayer.pdf and http://svitsrv25.epfl.ch/R-doc/library/rtracklayer/html/00Index.html

Thanks and best of luck for your research.

Other Answers
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Plz try Golden helix GnomeBrowser.

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Hi Anushree,

I am not very expert in it, but these wiki list https://en.wikipedia.org/wiki/Genome_browser could be useful. In addition, Python based genome viewer pybamview http://melissagymrek.com/python/2014/01/30/pybamview.html also seems promising.

Cheers

0

Hi Anu,

I found this interactive web application for visualizing genomic data http://chromozoom.org/ much useful.

Thanks