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LAST

http://last.cbrc.jp/

LAST can:

  • Handle big sequence data, e.g:
    • Compare two vertebrate genomes
    • Align billions of DNA reads to a genome
  • Indicate the reliability of each aligned column.
  • Use sequence quality data properly.
  • Compare DNA to proteins, with frameshifts.
  • Compare PSSMs to sequences
  • Calculate the likelihood of chance similarities between random sequences.
  • Do split and spliced alignment.
  • Train alignment parameters for unusual kinds of sequence (e.g. nanopore).