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  • Unicycler: Hybrid assembly pipeline for bacterial genomes

Unicycler: Hybrid assembly pipeline for bacterial genomes

https://github.com/rrwick/Unicycler

Unicycler is an assembly pipeline for bacterial genomes. It can assemble Illumina-only read sets where it functions as a SPAdes-optimiser. It can also assembly long-read-only sets (PacBio or Nanopore) where it runs a miniasm+Racon pipeline. For the best possible assemblies, give it both Illumina reads and long reads, and it will conduct a hybrid assembly.