Results for "Fastq"
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Cutadapt finds and removes adapter sequences, primers, poly-A tails and other types of unwanted sequence from your high-throughput sequencing reads. Cleaning your data in this way is often required: Reads from small-RNA sequencing contain the 3’ sequencing adapter because the read is longe...Tags: Bioinformatics, Cutadapt, NGS, Reads, Fastq, Sequencing
2895 days ago
WGS Celera Assembler version 8.3rc2
These are release notes for Celera Assembler version 8.3rc2, which was released on May 24, 2015.This distribution package provides a stable, tested, documented version of the software. The distribution is usable on most Unix-like platforms, and some platforms have pre-compiled binary distri...Tags: Bioinformatics, wgs-assembler, WGS, PBcR, Pacbio, Correct, Packages, Tools, Simulation, Fastq
2563 days ago
- FASTQ format is a text-based format for storing both a biological sequence (usually nucleotide sequence) and its corresponding quality scores. Both the sequence letter and quality score are each encoded with a single ASCII character for brevity. It was originally de...
Tags: Bioinformatics, NGS, Format, Fastq
2540 days ago
How to remove duplicates reads Ids ?
I mapped reads with bwa mem -M -t 40 allCombinedFinalSet.fa Seq.R1.fastq Seq.R2.fastq > aln.sam Extracted the mapped reads samtools view -f 0x2 -b aln.bam > output.bam Extracted the fastq bamToFastq -i output.bam -fq R1.fq -fq2 R2.fq grep @HISEQ578:1035:HJ2KCBCXX:1:1104...Tags: Reads, Ids, NGS, Fastq, Duplicates
2540 days ago
- Read one or more FASTQ files, fastqe will compute quality stats for each file and print those stats as emoji... for some reason. Given a fastq file in Illumina 1.8+/Sanger format, calculate the mean (rounded) score for each position and print a corresponding emoji! https://fastqe.co...
Tags: Fastq, stats, fastq, fastqe, Emoji
2080 days ago
- I wanted to remove the duplicates from fastq file. I planned to use fastuniq but it does not have good tutorial. Can you please tell me how to use it? http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0052249
Tags: fastq, fastuniq, duplicates, NGS
2541 days ago
Automatic Filtering, Trimming, Error Removing and Quality Control for fastq data
Automatic Filtering, Trimming, Error Removing and Quality Control for fastq dataAfterQC can simply go through all fastq files in a folder and then output three folders: good, bad and QC folders, which contains good reads, bad reads and the QC results of each fastq fi...Tags: Automatic, Filtering, Trimming, Error, Removing, Quality, Control, fastq, data
2346 days ago
