Next Generation Sequencing (NGS) Tutorials
...netic-inference.co.uk/blog/2009/04/basics-sequencing-dna-part-1/ http://www.genetic-inference.co.uk/blog/2009/08/basics-sequencing-dna-part-2/ A window into third-generation sequenc...3476 days ago
Trimmomatic: A flexible read trimming tool for Illumina NGS data
...ed.fq.gz ILLUMINACLIP:TruSeq3-PE.fa:2:30:10 LEADING:3 TRAILING:3 SLIDINGWINDOW:4:15 MINLEN:36 This will per...below quality 3) (TRAILING:3) Scan the read with a 4-base wide sliding window, cutting when the average qua...2931 days ago
Entrez Direct: E-utilities on the UNIX Command Line
...accessing the NCBI's suite of interconnected databases (publication, sequence, structure, gene, variation, expression, etc.) from a UNIX terminal window. Functions take search terms...2744 days ago
DnaSP v5: a software for comprehensive analysis of DNA polymorphism data
...for: (i) analyses on multiple data files; (ii) haplotype phasing; (iii) analyses on insertion/deletion polymorphism data; (iv) visualizing sliding window results integrated with avail...2634 days ago
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SvABA: Genome-wide detection of structural variants and indels by local assembly
...implementation of SGA (String Graph Assembler) by Jared Simpson, and BWA-MEM by Heng Li. Contigs are assembled for every 25kb window (with some small overlap) for...1919 days ago
mosdepth: fast BAM/CRAM depth calculation for WGS, exome, or targeted sequencing
mosdepth can output: per-base depth about 2x as fast samtools depth--about 25 minutes of CPU time for a 30X genome.mean per-window depth given a window size--as would be used for CNV calling.t...1620 days ago
Rcorrector: efficient and accurate error correction for Illumina RNA-seq reads
...INT: number of threads to use (default: 1) -trim : allow trimming (default: false) -maxcorK INT: the maximum number of correction within k-bp window (default: 4) -wk FLOAT: the...1540 days ago
SvABA: Structural variation and indel detection by local assembly
...implementation of SGA (String Graph Assembler) by Jared Simpson, and BWA-MEM by Heng Li. Contigs are assembled for every 25kb window (with some small overlap) for...1506 days ago