BBTools for bioinformatician !
...,nextera.fa.gz" and get them all (this will increase the amount of overtrimming, though it should still be ne...BBDuk.sh BBDuk can operate in one of 4 kmer-matching modes:Right-trimming (ktrim=r), left-trimming (ktr...2261 days ago
CANU genome assembly parameters !
...ter correction, trim and split based on consensus evidence. *.trimmedReads.fastq : file containing the sequences after correction and final trimming *.layout : file contain...1935 days ago
Protocol for De novo Genome Assembly using Illumina Reads
...e and what the standard is like. If required, raw data cleanup/quality trimming. Choose an adequate parameter...ticsonline.com/snippets/view/42540/install-fastqc-using-conda Quality trimming/cleanup of read files.This fu...1194 days ago
Illumina based assembly pipeline steps !
...ina Merge re-sequenced FastQ files (cat) Read QC (FastQC) Adapter trimming (fastp) Removal of host read...tersect variants across callers (BCFTools) De novo assembly Primer trimming (Cutadapt; amplicon data only...867 days ago