Results for "polishing"

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  • ONT assembly and Illumina polishing pipeline

    This pipeline performs the following steps: Assembly of nanopore reads using Canu. Polish canu contigs using racon (optional). Map a paired-end Illumina dataset onto the contigs obtained in the previous steps using BWA mem. Perform correction of contigs using p...

    Tags: ONT, assembly, Illumina, polishing, pipeline

    2318 days ago

  • Minipolish: A tool for Racon polishing of miniasm assemblies

    Miniasm is a great long-read assembly tool: straight-forward, effective and very fast. However, it does not include a polishing step, so its assemblies have a high error rate – they are essentially made of stitched-together pieces of long reads. Racon is a great polishing tool th...

    Tags: Minipolish, tool, Racon, polishing, miniasm, assemblies

    1578 days ago

  • Merfin: improved variant filtering, assembly evaluation and polishing via k-mer validation

    Merfin, a k-mer based variant-filtering algorithm for improved accuracy in genotyping and genome assembly polishing. Merfin evaluates each variant based on the expected k-mer multiplicity in the reads, independently of the quality of the read alignment and variant caller’s interna...

    Tags: Merfin, improved, variant, filtering, assembly, evaluation, polishing, k-mer, validation

    725 days ago

  • #Polishing PacBio assemblies with Arrow and Pilon https://flowersoftheocean.wordpress.com/2018/04/16/polishing-pacbio-assemblies-with-arrow-and-pilon/ #polish #Pilon

    Tags: Polishing, polish, Pilon

    1885 days ago

  • Apollo: A Sequencing-Technology-Independent, Scalable, and Accurate Assembly Polishing Algorithm

    Apollo is an assembly polishing algorithm that attempts to correct the errors in an assembly. It can take multiple set of reads in a single run and polish the assemblies of genomes of any size. Described by Firtina et al. (preliminary version at https://arxiv.org/pdf/1902.04341.pdf More at&...

    Tags: Apollo, Sequencing, Technology, Independent, Scalable, Accurate, Assembly, Polishing, Algorithm

    1474 days ago