Results for "reads"

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  • shovill: Assemble bacterial isolate genomes from Illumina paired-end reads

    Shovill is a pipeline which uses SPAdes at its core, but alters the steps before and after the primary assembly step to get similar results in less time. Shovill also supports other assemblers like SKESA, Velvet and Megahit, so you can take advantage of the pre- and post-processing the Shovill pr...

    Tags: shovill, Assemble, bacterial, isolate, genomes, Illumina, paired-end, reads

    1182 days ago

  • Metagenomics

    Metagenomics

    Metagenomics is the study of genetic material from environmental samples to characterize and identify the millions of microbes from these samples. Example: Gut microbiome, soil sample, water, etc. More information: http://www.nature.com/nrmicro/focus/metagenomics/index.html http://www.ploscom...

    Tags: metagenomics, metatranscriptomics, genomics, reads, 16S rRNA, microbes, microbiology, ecogenomics, ecology, microbiome

    3666 days ago

  • #Mapping #sequencing #reads to a #reference #genome with #Bowtie2: a step-by-step guide https://insidedna.me/tutorials/view/how-to-use-bowtie2-read-mapping

    Tags: Mapping, sequencing, reads, reference, genome, Bowtie2

    2938 days ago

  • GraphMap - A highly sensitive and accurate mapper for long, error-prone reads

    GraphMap - A highly sensitive and accurate mapper for long, error-prone reads http://www.nature.com/ncomms/2016/160415/ncomms11307/full/ncomms11307.htmlFeatures    Mapping position agnostic to alignment parameters.    Consistently very high sensitivity and precision ...

    Tags: GraphMap, sensitive, accurate, mapper, long, error-prone, reads

    2487 days ago

  • #Sickle ( https://github.com/najoshi/sickle ) to #trim #reads using a 20-Phred quality threshold

    Tags: Sickle, trim, reads

    2454 days ago

  • #Filter out #reads with #size to discard reads containing many Ns. BBNorm ( https://sourceforge.net/projects/bbmap/ )

    Tags: Filter, reads, size

    2454 days ago

  • Get the reads in fastq file: awk '{s++}END{print s/4}' file.fastq #reads #count #ngs #fastq

    Tags: reads, count, ngs, fastq

    2333 days ago

  • miniasm: very fast OLC-based de novo assembler for noisy long reads

    Miniasm is a very fast OLC-based de novo assembler for noisy long reads. It takes all-vs-all read self-mappings (typically by minimap) as input and outputs an assembly graph in the GFA format. Different from mainstream assemblers, miniasm does not have a consensus step. I...

    Tags: Ultrafast, de novo, assembly, long, noisy, reads, consensus

    2314 days ago

  • INC-Seq: accurate single molecule reads using nanopore sequencing

    INC-Seq reads enabled accurate species-level classification, identification of species at 0.1 % abundance and robust quantification of relative abundances, providing a cheap and effective approach for pathogen detection and microbiome profiling on the MinION system.

    Tags: INC-Seq, accurate, single, molecule, reads, nopore, sequencing

    2314 days ago

  • COPE: an accurate k-mer-based pair-end reads connection tool to facilitate genome assembly

    An efficient tool called Connecting Overlapped Pair-End (COPE) reads, to connect overlapping pair-end reads using k-mer frequencies. We evaluated our tool on 30× simulated pair-end reads from Arabidopsis thaliana with 1% base error. COPE connected over 99% of reads with 98.8% accuracy, whic...

    Tags: COPE, accurate, k-mer, pair-end, reads, connection, tool, genome, assembly

    2305 days ago