<![CDATA[BOL: Can anyone tell me the clear procedure for new or novel miRNA identification in animal??]]> https://bioinformaticsonline.com/answers/view/13482/can-anyone-tell-me-the-clear-procedure-for-new-or-novel-mirna-identification-in-animal? https://bioinformaticsonline.com/answers/view/13482/can-anyone-tell-me-the-clear-procedure-for-new-or-novel-mirna-identification-in-animal Thu, 07 Aug 2014 04:13:43 -0500 https://bioinformaticsonline.com/answers/view/13482/can-anyone-tell-me-the-clear-procedure-for-new-or-novel-mirna-identification-in-animal <![CDATA[Can anyone tell me the clear procedure for new or novel miRNA identification in animal??]]> Hi!

Currently i am working in miRNA identification based on EST of particular animal.. i did blastn search against the EST to identify the homologous of mature miRNA.. Next step is blastx to remove the protein coding sequences. How can i select the non-coding transcripts or EST's from those results? On what criteria i have to select?? Then I want to know how to select the range of precursors and mature miRNA? Based on secondary structure means how they find the mature miRNA? I read many articles but i am still not clear how to find those.. If anyone know plz help me..

Thanks,

Nandhini

]]> Nandhini devi