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	<title><![CDATA[BOL: ScaffMatch]]></title>
	<link>https://bioinformaticsonline.com/bookmarks/view/30130/scaffmatch?</link>
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	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/30130/scaffmatch</guid>
	<pubDate>Tue, 13 Dec 2016 10:23:56 -0600</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/30130/scaffmatch</link>
	<title><![CDATA[ScaffMatch]]></title>
	<description><![CDATA[<p>caffMatch is a novel scaffolding tool based on Maximum-Weight Matching able to produce high-quality scaffolds from NGS data (reads and contigs). The tool is written in Python 2.7. It also includes a bash script wrapper that calls aligner in case one needs to first map reads to contigs (instead of providing .sam files).</p>
<p>The arguments accepted by ScaffMatch are:</p>
<p>&nbsp; -w) Working directory -- this is the directory where ScaffMatch files are stored. These are .sam files produced after mapping reads to contigs and the resulting scaffolds file `scaffolds.fa` fasta file;</p>
<p>&nbsp; -c) Contig fasta file;</p>
<p>&nbsp; -m) Command line argument with no options. It is used when .sam files are used instead of reads .fastq files. Do not use this option if you provide reads files;</p>
<p>&nbsp; -1) (Comma separated list of) either .fastq or .sam file(s) corresponding to the first read of the read pair;</p>
<p>&nbsp; -2) (Comma separated list of) either .fastq or .sam file(s) corresponding to the second read of the read pair;</p>
<p>&nbsp; -i) (Comma separated list of) insert size(s) of the library(-ies);</p>
<p>&nbsp; -s) (Comma separated list of) library(-ies) standard deviation(s) of insert size(s);</p>
<p>&nbsp; -t) Bundle threshold. Pairs of contigs supported by number of read pairs less than the value of this argument are discarded. Optional argument, by default it is equal to 5;</p>
<p>&nbsp; -g) Matching heuristics: use `max_weight` for Maximum Weight Matching heuristics with the Insertion step, use `backbone` for Maximum Weight Matching heuristics without the Insertion step, use `greedy` for Greedy Matching heuristics;</p>
<p>&nbsp; -l) Log file - where to store the logs. Optional argument. By default, stdout is used.</p><p>Address of the bookmark: <a href="http://alan.cs.gsu.edu/NGS/?q=content/scaffmatch" rel="nofollow">http://alan.cs.gsu.edu/NGS/?q=content/scaffmatch</a></p>]]></description>
	<dc:creator>Jit</dc:creator>
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