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	<title><![CDATA[BOL: A Post-assembly genome-improvement toolkit (PAGIT) to obtain annotated genomes from contigs]]></title>
	<link>https://bioinformaticsonline.com/bookmarks/view/32633/a-post-assembly-genome-improvement-toolkit-pagit-to-obtain-annotated-genomes-from-contigs?</link>
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	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/32633/a-post-assembly-genome-improvement-toolkit-pagit-to-obtain-annotated-genomes-from-contigs</guid>
	<pubDate>Fri, 12 May 2017 10:50:29 -0500</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/32633/a-post-assembly-genome-improvement-toolkit-pagit-to-obtain-annotated-genomes-from-contigs</link>
	<title><![CDATA[A Post-assembly genome-improvement toolkit (PAGIT) to obtain annotated genomes from contigs]]></title>
	<description><![CDATA[<p>PAGIT addresses the need for software to generate high quality draft genomes. It is based on a series of programs that we developed:</p>
<p><a href="https://sourceforge.net/projects/abacas/files/">ABACAS</a>, that is able to contiguate contigs from a de novo assembly against a closely related reference.</p>
<p><a href="https://sourceforge.net/projects/image2/files/">IMAGE</a>, an iterative approach for closing gaps in assembled genomes using mate pair information. It is able to close gaps left open by the assembler in a draft genome, even when using the same data sets as used by the original assembler.</p>
<p><a href="http://icorn.sourceforge.net/">iCORN</a>, that enables errors in the consensus sequence to be corrected by iteratively mapping reads to the current assembly. An improved version, especially correction Pacfic Bioscience assemblies (PacBio) can be found&nbsp;<a href="ftp://ftp.sanger.ac.uk/pub4/resources/software/pagit/ICORN2/icorn2.V0.95.tgz">here</a>.</p>
<p><a href="https://ratt.svn.sourceforge.net/svnroot/ratt">RATT</a>, a tool to transfer the annotation from a reference genome, or an earlier assembly, onto the latest assembly.</p>
<p>PAGIT bundles these software and makes them more accessible for users.</p><p>Address of the bookmark: <a href="http://www.sanger.ac.uk/science/tools/pagit" rel="nofollow">http://www.sanger.ac.uk/science/tools/pagit</a></p>]]></description>
	<dc:creator>Abhimanyu Singh</dc:creator>
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