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	<title><![CDATA[BOL: ONT assembly and Illumina polishing pipeline]]></title>
	<link>https://bioinformaticsonline.com/bookmarks/view/34398/ont-assembly-and-illumina-polishing-pipeline?</link>
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	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/34398/ont-assembly-and-illumina-polishing-pipeline</guid>
	<pubDate>Thu, 23 Nov 2017 10:13:42 -0600</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/34398/ont-assembly-and-illumina-polishing-pipeline</link>
	<title><![CDATA[ONT assembly and Illumina polishing pipeline]]></title>
	<description><![CDATA[<p>This pipeline performs the following steps:</p>
<ul>
<li>Assembly of nanopore reads using&nbsp;<a href="http://canu.readthedocs.io/">Canu</a>.</li>
<li>Polish canu contigs using&nbsp;<a href="https://github.com/isovic/racon">racon</a>&nbsp;(<em>optional</em>).</li>
<li>Map a paired-end Illumina dataset onto the contigs obtained in the previous steps using&nbsp;<a href="http://bio-bwa.sourceforge.net/">BWA</a>&nbsp;mem.</li>
<li>Perform correction of contigs using&nbsp;<a href="https://github.com/broadinstitute/pilon/wiki">pilon</a>&nbsp;and the Illumina dataset.</li>
</ul><p>Address of the bookmark: <a href="https://github.com/nanoporetech/ont-assembly-polish" rel="nofollow">https://github.com/nanoporetech/ont-assembly-polish</a></p>]]></description>
	<dc:creator>Jit</dc:creator>
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