<![CDATA[BOL: SimLoRD: A read simulator for third generation sequencing reads]]> https://bioinformaticsonline.com/bookmarks/view/37574/simlord-a-read-simulator-for-third-generation-sequencing-reads? https://bioinformaticsonline.com/bookmarks/view/37574/simlord-a-read-simulator-for-third-generation-sequencing-reads Wed, 22 Aug 2018 10:40:27 -0500 https://bioinformaticsonline.com/bookmarks/view/37574/simlord-a-read-simulator-for-third-generation-sequencing-reads <![CDATA[SimLoRD: A read simulator for third generation sequencing reads]]> SimLoRD is a read simulator for third generation sequencing reads and is currently focused on the Pacific Biosciences SMRT error model.

Reads are simulated from both strands of a provided or randomly generated reference sequence.

  • The reference can be read from a FASTA file or randomly generated with a given GC content. It can consist of several chromosomes, whose structure is respected when drawing reads. (Simulation of genome rearrangements may be incorporated at a later stage.)
  • The read lengths can be determined in four ways: drawing from a log-normal distribution (typical for genomic DNA), sampling from an existing FASTQ file (typical for RNA), sampling from a a text file with integers (RNA), or using a fixed length
  • Quality values and number of passes depend on fragment length.
  • Provided subread error probabilities are modified according to number of passes
  • Outputs reads in FASTQ format and alignments in SAM format

Address of the bookmark: https://bitbucket.org/genomeinformatics/simlord/

]]> Aaryan Lokwani