<![CDATA[BOL: Roary: the Pan Genome Pipeline]]> https://bioinformaticsonline.com/bookmarks/view/38758/roary-the-pan-genome-pipeline? https://bioinformaticsonline.com/bookmarks/view/38758/roary-the-pan-genome-pipeline Tue, 22 Jan 2019 05:52:07 -0600 https://bioinformaticsonline.com/bookmarks/view/38758/roary-the-pan-genome-pipeline <![CDATA[Roary: the Pan Genome Pipeline]]> Roary is a high speed stand alone pan genome pipeline, which takes annotated assemblies in GFF3 format (produced by Prokka (Seemann, 2014)) and calculates the pan genome. Using a standard desktop PC, it can analyse datasets with thousands of samples, something which is computationally infeasible with existing methods, without compromising the quality of the results. 128 samples can be analysed in under 1 hour using 1 GB of RAM and a single processor. To perform this analysis using existing methods would take weeks and hundreds of GB of RAM. Roary is not intended for meta-genomics or for comparing extremely diverse sets of genomes.

Address of the bookmark: https://sanger-pathogens.github.io/Roary/

]]> Jit https://bioinformaticsonline.com/bookmarks/view/38758/roary-the-pan-genome-pipeline#item-annotation-3579 Tue, 22 Jan 2019 06:15:46 -0600 https://bioinformaticsonline.com/bookmarks/view/38758/roary-the-pan-genome-pipeline#item-annotation-3579 <![CDATA[Comment by Jit]]> Tutorial at http://sepsis-omics.github.io/tutorials/modules/roary/

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