<![CDATA[BOL: DADA2: Fast and accurate sample inference from amplicon data with single-nucleotide resolution]]> https://bioinformaticsonline.com/bookmarks/view/42310/dada2-fast-and-accurate-sample-inference-from-amplicon-data-with-single-nucleotide-resolution? https://bioinformaticsonline.com/bookmarks/view/42310/dada2-fast-and-accurate-sample-inference-from-amplicon-data-with-single-nucleotide-resolution Tue, 10 Nov 2020 20:26:00 -0600 https://bioinformaticsonline.com/bookmarks/view/42310/dada2-fast-and-accurate-sample-inference-from-amplicon-data-with-single-nucleotide-resolution <![CDATA[DADA2: Fast and accurate sample inference from amplicon data with single-nucleotide resolution]]> The DADA2 tutorial goes through a typical workflow for paired end Illumina Miseq data: raw amplicon sequencing data is processed into the table of exact amplicon sequence variants (ASVs) present in each sample.

The DADA2 Workflow on Big Data goes through workflow optimized to run on large datasets (10s of millions to billions of reads).

An ITS-specific version of the DADA2 workflow identifies and verifiably removes primers on both ends of each ITS read, a key step due to the variable length of the ITS region.

Short demonstrations of assigning taxonomy and assigning species to sequences.

Address of the bookmark: https://benjjneb.github.io/dada2/index.html

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