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	<title><![CDATA[BOL: Download blasr 1.3 version]]></title>
	<link>https://bioinformaticsonline.com/file/view/36945/download-blasr-13-version?</link>
	<atom:link href="https://bioinformaticsonline.com/file/view/36945/download-blasr-13-version?" rel="self" type="application/rss+xml" />
	<description><![CDATA[]]></description>
	
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	<guid isPermaLink="true">https://bioinformaticsonline.com/file/view/36945/download-blasr-13-version</guid>
	<pubDate>Fri, 15 Jun 2018 03:01:20 -0500</pubDate>
	<link>https://bioinformaticsonline.com/file/view/36945/download-blasr-13-version</link>
	<title><![CDATA[Download blasr 1.3 version]]></title>
	<description><![CDATA[<p>DOWNLOAD LINK: https://github.com/BioInf-Wuerzburg/proovread/raw/master/util/blasr-1.3.1/blasr</p><p>I'm running "OPERA-LG_v2.0.5/bin/preprocess_reads.pl" and have the following error:</p><p>fail to open file './temporarySam'</p><p><br />[bwa_aln_core] write to the disk... 0.09 sec<br />[bwa_aln_core] 70778880 sequences have been processed.<br />[bwa_aln_core] calculate SA coordinate... 161.35 sec<br />[bwa_aln_core] write to the disk... 0.06 sec<br />[bwa_aln_core] 70989574 sequences have been processed.<br />[main] Version: 0.7.15-r1140<br />[main] CMD: bwa aln -t 30 all_p_ctg.fa -<br />[main] Real time: 2402.523 sec; CPU: 53429.488 sec<br />[E::hts_open_format] Failed to open file temporarySam<br />samtools sort: can't open "temporarySam": No such file or directory<br />[bwa_aln_core] convert to sequence coordinate... 1.00 sec<br />[bwa_aln_core] refine gapped alignments... 6.07 sec<br />[bwa_aln_core] print alignments... PREPROCESS:<br />Fastq format is recognized<br />[Thu Jun 14 18:16:47 2018] Building bwa index...<br />bwa index -p all_p_ctg.fa /home/urbe/Tools/OPERA-LG_v2.0.6/all_p_ctg.fa<br />[Thu Jun 14 18:18:35 2018] Finding the SA coordinates of the reads using BWA aln...<br />[Thu Jun 14 18:58:37 2018] Generate alignments of reads using bwa sampe...<br />bwa samse -n 1 all_p_ctg.fa read.sai - | grep '\(^@\|XT:A:U\)' | /usr/local/bin/samtools view -S -h -b -F 0x4 - | /usr/local/bin/samtools sort -@ 20 -no - temporarySam &gt; FALCON-Unzip-Scaff.bam<br />Mapping long-reads using blasr...<br />/home/urbe/Tools/SSpace/SSPACE-LongRead_v1-1/blasr -nproc 40 -m 1 -minMatch 5 -bestn 10 -noSplitSubreads -advanceExactMatches 1 -nCandidates 1 -maxAnchorsPerPosition 1 -sdpTupleSize 7 /media/urbe/MyDDrive/ONTdata/allONT/allONT.fasta /home/urbe/Tools/OPERA-LG_v2.0.6/all_p_ctg.fa | cut -d ' ' -f1-5,7-12 | sed 's/ /\t/g' &gt; FALCON-Unzip-Scaff.map<br />sh: 1: /home/urbe/Tools/SSpace/SSPACE-LongRead_v1-1/blasr: Permission denied<br />Sorting mapping results...<br />sort -k1,1 -k9,9g FALCON-Unzip-Scaff.map &gt; FALCON-Unzip-Scaff.map.sort<br />Analyzing sorted results...<br />Extracting linking information...<br />i3 2000 5000<br />i2 1000 2000<br />i4 5000 15000<br />i0 -200 300<br />i5 15000 40000<br />i1 300 1000<br />Repeat detection...<br />/home/urbe/Tools/OPERA-LG_v2.0.6/bin//filter_conflicting_edge.pl pairedEdges_i0 contig_length.dat 100 2<br />Illegal division by zero at /home/urbe/Tools/OPERA-LG_v2.0.6/bin//filter_conflicting_edge.pl line 93.<br />readline() on closed filehandle FILE at bin/OPERA-long-read.pl line 250.<br />rm anchor_contig_info.dat contig_length.dat filtered_edges.dat filtered_edges_cov.dat *.sai<br />rm: cannot remove 'anchor_contig_info.dat': No such file or directory<br />mv FALCON-Unzip-Scaff.bam FALCON-Unzip-Scaff-with-repeat.bam<br />/home/urbe/Tools/OPERA-LG_v2.0.6/bin//filter_repeat.pl FALCON-Unzip-Scaff-with-repeat.bam repeat.dat | /usr/local/bin/samtools view - -h -S -b &gt; FALCON-Unzip-Scaff.bam<br />rm FALCON-Unzip-Scaff-with-repeat.bam<br />/home/urbe/Tools/OPERA-LG_v2.0.6/bin/OPERA-LG config &gt; log<br />Analyzing 1 library: FALCON-Unzip-Scaff.bam<br />min library mean : 0<br />minimum contig length is 500<br />Current library: 1 out of 7<br />Analyzing file: pairedEdges_no_repeat_i0<br />Analyzing file: pairedEdges_no_repeat_i1<br />Analyzing file: pairedEdges_no_repeat_i2<br />Analyzing file: pairedEdges_no_repeat_i3<br />Analyzing file: pairedEdges_no_repeat_i4<br />Analyzing file: pairedEdges_no_repeat_i5<br />ln -s results/scaffoldSeq.fasta scaffoldSeq.fasta</p><p>To resolve this, try downloading blasr version 1.3 above and re-run :)</p>]]></description>
	<dc:creator>Jit</dc:creator>
	<enclosure url="https://bioinformaticsonline.com/file/download/36945" length="0" type="inode/x-empty" />
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