<![CDATA[BOL: Primer BLAST !]]> https://bioinformaticsonline.com/news/view/41586/primer-blast? https://bioinformaticsonline.com/news/view/41586/primer-blast Tue, 28 Apr 2020 00:28:49 -0500 https://bioinformaticsonline.com/news/view/41586/primer-blast <![CDATA[Primer BLAST !]]> BLAST team added a new feature (Max 3' match), shown in Figure 1, to Primer-BLAST that limits the length of 3' exon matches when designing exon-exon spanning primers. This makes it less likely that primers specifically designed to amplify transcripts will also amplify genomic DNA contamination in expression assays. See the NCBI Insights post (https://go.usa.gov/xvUT4) for more details.

 

If you have any questions or concerns, please contact blast-help@ncbi.nlm.nih.govimage

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