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	<title><![CDATA[BOL: Related items]]></title>
	<link>https://bioinformaticsonline.com/related/11175?</link>
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	<description><![CDATA[]]></description>
	
	<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/3868/next-generation-sequencing-ngs-tutorials</guid>
	<pubDate>Sat, 24 Aug 2013 06:01:37 -0500</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/3868/next-generation-sequencing-ngs-tutorials</link>
	<title><![CDATA[Next Generation Sequencing (NGS) Tutorials]]></title>
	<description><![CDATA[<p>Institute of computational biomedicine, Cornell University provide an NGS workshop tutorial at&nbsp;<a href="http://chagall.med.cornell.edu/NGScourse/">http://chagall.med.cornell.edu/NGScourse/</a>&nbsp;</p>
<p>You can also add your favourite NGS educational material, or workshop tutorial by commenting on this bookmarks for user benefit.&nbsp;</p>
<p>Understanding the basics of genome sequencing:</p>
<p>Tutorial by Luke Jostins.</p>
<p>http://www.genetic-inference.co.uk/blog/2009/04/basics-sequencing-dna-part-1/</p>
<p>http://www.genetic-inference.co.uk/blog/2009/08/basics-sequencing-dna-part-2/</p>
<p>A window into third-generation sequencing</p>
<p>http://hmg.oxfordjournals.org/content/19/R2/R227.full.pdf</p>
<p>==============================================</p>
<p>NGS data analysis pipelines</p>
<ul>
<li><strong>Detecting and annotating genetic variations using the HugeSeq pipeline</strong>&nbsp; DOI: <a href="http://dx.doi.org/10.1038/nbt.2134">10.1038/nbt.2134</a></li>
<li><strong> NARWHAL, a primary analysis pipeline for NGS data</strong> <a href="http://bioinformatics.oxfordjournals.org/cgi/content/abstract/28/2/284?etoc">http://bioinformatics.oxfordjournals.org/cgi/content/abstract/28/2/284?etoc</a></li>
<li><strong>RseqFlow: Workflows for RNA-Seq data analysis</strong>&nbsp; DOI: <a href="http://dx.doi.org/10.1093/bioinformatics/btr441">10.1093/bioinformatics/btr441</a></li>
<li><strong>ngs_backbone: a pipeline for read cleaning, mapping and SNP calling using Next Generation Sequence</strong>&nbsp;&nbsp;<a href="http://dx.doi.org/10.1186/1471-2164-12-285">10.1186/1471-2164-12-285</a></li>
<li><strong>A framework for variation discovery and genotyping using next-generation DNA sequencing data</strong>&nbsp; PubMed: <a href="http://www.ncbi.nlm.nih.gov/pubmed/21478889">21478889</a></li>
<li><strong>SNiPlay: a web-based tool for detection, management and analysis of SNPs. Application to grapevine diversity projects</strong>&nbsp; DOI: <a href="http://dx.doi.org/10.1186/1471-2105-12-134">10.1186/1471-2105-12-134</a> Abstract: <a href="http://www.biomedcentral.com/1471-2105/12/134/abstract">http://www.biomedcentral.com/1471-2105/12/134/abstract</a></li>
<li><strong>WEP: a high-performance analysis pipeline for whole-exome data&nbsp;</strong>http://www.biomedcentral.com/1471-2105/14/S7/S11</li>
<li><strong>DDBJ read annotation pipeline: a cloud computing-based pipeline for high-throughput analysis of next-generation sequencing data.&nbsp;</strong>http://www.ncbi.nlm.nih.gov/pubmed/23657089</li>
<li><strong>GATK: a Toolkit for Genome Analysis&nbsp;</strong>http://www.broadinstitute.org/gatk/</li>
<li><strong>Metagenomics</strong>:http://www.nbic.nl/education/nbic-phd-school/course-schedule/ngsmetagenomics/</li>
<li><strong>RNASeq</strong>:http://www.nbic.nl/education/nbic-phd-school/course-schedule/ngsrnaseq/</li>
<li><strong>Bioinformatics and Seq courses</strong>:&nbsp;http://www.isb-sib.ch/training/training-activities-schedule/archive-2013.html</li>
<li><strong>Variant Detection (Model organism) Advanced tutorial</strong> https://docs.google.com/document/pub?id=1CuKkKylVDb03tnN7RSWl5EUzleetn0ctjmvaidPKLxM</li>
<li><strong>Variant Detection Introductory tutorial</strong> https://docs.google.com/document/pub?id=1ZRzrjjOCvtAu3m-IKL-rbJ1f4On60dDL_IEwG7oejdI</li>
<li><strong>Microbial de novo Assembly for Illumina Data Introductory tutorial</strong> https://docs.google.com/document/pub?id=1N3AB9ptISUu4zULqe1kXpVF0BDyGb5f5yzxWSJd_WNM</li>
<li><strong>RNAseq Differential Gene Expression Introductory tutorial</strong> https://docs.google.com/document/pub?id=1KbTiBHtvHLfPRZ39AY3uriazrINA8TJzgjjwn1zPP7Y</li>
</ul>
<blockquote>
<p>" Please add your favourite NGS link below in comment section for the benefit of bioinformatics community ".&nbsp;</p>
</blockquote><p>Address of the bookmark: <a href="http://chagall.med.cornell.edu/NGScourse/" rel="nofollow">http://chagall.med.cornell.edu/NGScourse/</a></p>]]></description>
	<dc:creator>Jitendra Narayan</dc:creator>
</item>
<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/26309/ratt</guid>
	<pubDate>Sun, 07 Feb 2016 16:09:40 -0600</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/26309/ratt</link>
	<title><![CDATA[RATT]]></title>
	<description><![CDATA[<p><strong>RATT</strong> is software to transfer annotation from a reference (annotated) genome to an unannotated query genome.</p>
<p>It was first developed to transfer annotations between different genome assembly versions. However, it can also transfer annotations between strains and even different species, like <em>Plasmodium chabaudi</em> onto <em> P. berghei</em>, between different Leishmania species or <em>Salmonella enterica</em> onto other Salmonella serotypes. <strong>RATT</strong> is able to transfer any entries present on a reference sequence, such as the systematic id or an annotator's notes; such information would be lost in a <em>de novo</em> annotation.</p>
<p>More at http://ratt.sourceforge.net/</p><p>Address of the bookmark: <a href="http://ratt.sourceforge.net/" rel="nofollow">http://ratt.sourceforge.net/</a></p>]]></description>
	<dc:creator>Jitendra Narayan</dc:creator>
</item>
<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/26972/understanding-fastqc-output</guid>
	<pubDate>Fri, 15 Apr 2016 05:47:40 -0500</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/26972/understanding-fastqc-output</link>
	<title><![CDATA[Understanding Fastqc Output]]></title>
	<description><![CDATA[<p>Understanding Following table and graphs</p>
<ol>
<li>Duplication level</li>
<li>kmer profile</li>
<li>per base GC content</li>
<li>per base N content</li>
<li>per base quality</li>
<li>per base sequence content</li>
<li>per sequence GC content</li>
<li>per sequence quality</li>
<li>sequence length distribution</li>
</ol>
<p>More at http://www.bioinformatics.babraham.ac.uk/projects/fastqc/Help/3%20Analysis%20Modules/</p><p>Address of the bookmark: <a href="http://www.bioinformatics.babraham.ac.uk/projects/fastqc/Help/3%20Analysis%20Modules/" rel="nofollow">http://www.bioinformatics.babraham.ac.uk/projects/fastqc/Help/3%20Analysis%20Modules/</a></p>]]></description>
	<dc:creator>Jit</dc:creator>
</item>
<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/27099/rasttk-algorithm-for-building-custom-annotation-pipelines-and-annotating-batches-of-genomes</guid>
	<pubDate>Wed, 27 Apr 2016 11:07:59 -0500</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/27099/rasttk-algorithm-for-building-custom-annotation-pipelines-and-annotating-batches-of-genomes</link>
	<title><![CDATA[RASTtk : algorithm for building custom annotation pipelines and annotating batches of genomes]]></title>
	<description><![CDATA[<p>The RAST (Rapid Annotation using Subsystem Technology) annotation engine was built in 2008 to annotate bacterial and archaeal genomes. It works by offering a standard software pipeline for identifying genomic features (i.e., protein-encoding genes and RNA) and annotating their functions. Recently, in order to make RAST a more useful research tool and to keep pace with advancements in bioinformatics, it has become desirable to build a version of RAST that is both customizable and extensible. In this paper, we describe the RAST tool kit (RASTtk), a modular version of RAST that enables researchers to build custom annotation pipelines. RASTtk offers a choice of software for identifying and annotating genomic features as well as the ability to add custom features to an annotation job. RASTtk also accommodates the batch submission of genomes and the ability to customize annotation protocols for batch submissions. This is the first major software restructuring of RAST since its inception.</p>
<p>More at http://www.nature.com/articles/srep08365</p><p>Address of the bookmark: <a href="http://rast.nmpdr.org/" rel="nofollow">http://rast.nmpdr.org/</a></p>]]></description>
	<dc:creator>Abhi</dc:creator>
</item>
<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/27967/linux-command-line-exercises-for-ngs-data-processing</guid>
	<pubDate>Wed, 22 Jun 2016 07:59:39 -0500</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/27967/linux-command-line-exercises-for-ngs-data-processing</link>
	<title><![CDATA[Linux command line exercises for NGS data processing]]></title>
	<description><![CDATA[<p>The purpose of this tutorial is to introduce students to the frequently used tools for NGS analysis as well as giving experience in writing one-liners. Copy the required files to your current directory, change directory (<code>cd</code>) to the <code>linuxTutorial</code> folder, and do all the processing inside:</p>
<pre><span>[uzi@quince-srv2 ~/]$</span> cp -r /home/opt/MScBioinformatics/linuxTutorial .
<span>[uzi@quince-srv2 ~/]$</span> cd linuxTutorial
<span>[uzi@quince-srv2 ~/linuxTutorial]$</span>
</pre>
<p>I have deliberately chosen <code>Awk</code> in the exercises as it is a language in itself and is used more often to manipulate NGS data as compared to the other command line tools such as <code>grep</code>, <code>sed</code>, <code>perl</code> etc. Furthermore, having a command on <code>awk</code> will make it easier to understand advanced tutorials such as <a href="http://userweb.eng.gla.ac.uk/umer.ijaz/bioinformatics/Illumina_workflow.html">Illumina Amplicons Processing Workflow</a>. <br><br> In <code>Linux</code>, we use a shell that is a program that takes your commands from the keyboard and gives them to the operating system. Most Linux systems utilize Bourne Again SHell (<code>bash</code>), but there are several additional shell programs on a typical Linux system such as <code>ksh</code>, <code>tcsh</code>, and <code>zsh</code>. To see which shell you are using, type</p>
<pre><span>[uzi@quince-srv2 ~/linuxTutorial]$</span> echo $SHELL

<span>/bin/bash
</span></pre><p>Address of the bookmark: <a href="http://userweb.eng.gla.ac.uk/umer.ijaz/bioinformatics/linux.html" rel="nofollow">http://userweb.eng.gla.ac.uk/umer.ijaz/bioinformatics/linux.html</a></p>]]></description>
	<dc:creator>Jit</dc:creator>
</item>
<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/28844/teannot</guid>
	<pubDate>Thu, 18 Aug 2016 10:02:03 -0500</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/28844/teannot</link>
	<title><![CDATA[TEannot]]></title>
	<description><![CDATA[<p>We advise to run first the TEdenovo pipeline but it is not compulsory. We suppose you begin by running the TEannot pipeline on the example provided in the directory "db/" rather than directly on your own genomic sequences. Thus, from now on, the project name is "DmelChr4".</p>
<p>&nbsp;</p><p>Address of the bookmark: <a href="https://urgi.versailles.inra.fr/Tools/REPET/TEannot-tuto" rel="nofollow">https://urgi.versailles.inra.fr/Tools/REPET/TEannot-tuto</a></p>]]></description>
	<dc:creator>Jit</dc:creator>
</item>
<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/36880/jvarkit-java-utilities-for-bioinformatics</guid>
	<pubDate>Fri, 08 Jun 2018 09:31:55 -0500</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/36880/jvarkit-java-utilities-for-bioinformatics</link>
	<title><![CDATA[Jvarkit : Java utilities for Bioinformatics]]></title>
	<description><![CDATA[Collection of Java tool kits for bioinformatics works:

Jvarkit : Java utilities for Bioinformatics<p>Address of the bookmark: <a href="http://lindenb.github.io/jvarkit/" rel="nofollow">http://lindenb.github.io/jvarkit/</a></p>]]></description>
	<dc:creator>Jit</dc:creator>
</item>
<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/file/view/29638/r-graphical-cookbook-by-winston-chang</guid>
	<pubDate>Fri, 04 Nov 2016 12:50:30 -0500</pubDate>
	<link>https://bioinformaticsonline.com/file/view/29638/r-graphical-cookbook-by-winston-chang</link>
	<title><![CDATA[R Graphical Cookbook by Winston Chang]]></title>
	<description><![CDATA[<p>R Graphical Cookbook by Winston Chang</p><p>A very nice book by Winston Chang for R ethusiast. The R code presented in these pages is the R code actually used to produce the Figures in the book. There will be differences compared to the code chunks shown in the text of the book, but in most cases the differences will be that these pages contain additional code to lay out multiple plots on a single "page".</p><p>The code presented for each figure is self-contained, i.e., all code required to produce the figure is included. This means that there is sometimes considerable overlap of code between several figures  In some cases, it may be necessary to install an add-on package from CRAN to get the code to run.</p><p>More books at http://www.e-reading.club/bookreader.php/137370/C486x_APPb.pdf</p>]]></description>
	<dc:creator>Abhimanyu Singh</dc:creator>
	<enclosure url="https://bioinformaticsonline.com/file/download/29638" length="37521" type="image/png" />
</item>
<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/27104/gatb-genome-analysis-toolbox-with-de-bruijn-graph</guid>
	<pubDate>Thu, 28 Apr 2016 11:16:51 -0500</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/27104/gatb-genome-analysis-toolbox-with-de-bruijn-graph</link>
	<title><![CDATA[GATB : Genome Analysis Toolbox with de-Bruijn graph]]></title>
	<description><![CDATA[<p>The&nbsp;<strong><strong>Genome Analysis Toolbox with de-Bruijn graph</strong> (GATB)</strong> provides a set of <a href="https://gatb.inria.fr/gatb-global-architecture/">highly efficient algorithms to analyse NGS data sets</a>. These methods enable the analysis of data sets of any size on multi-core desktop computers, including very huge amount of reads data coming from any kind of organisms such as bacteria, plants, animals and even complex samples (<em>e.g.</em> metagenomes).</p>
<p>More at https://gatb.inria.fr/</p><p>Address of the bookmark: <a href="https://gatb.inria.fr/" rel="nofollow">https://gatb.inria.fr/</a></p>]]></description>
	<dc:creator>Jit</dc:creator>
</item>
<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/27971/samtools-primer</guid>
	<pubDate>Thu, 23 Jun 2016 07:18:17 -0500</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/27971/samtools-primer</link>
	<title><![CDATA[Samtools Primer !!]]></title>
	<description><![CDATA[<p>SAMtools: Primer / Tutorial by Ethan Cerami, Ph.D.<br><br>keywords: samtools, next-gen, next-generation, sequencing, bowtie, sam, bam, primer, tutorial, how-to, introduction<br>Revisions<br><br>&nbsp;&nbsp;&nbsp; 1.0: May 30, 2013: First public release on biobits.org.<br>&nbsp;&nbsp;&nbsp; 1.1: July 24, 2013: Updated with Disqus Comments / Feedback section.<br>&nbsp;&nbsp;&nbsp; 1.2: December 19, 2014: Multiple updates, including:<br>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Updated to use samtools 1.1 and bcftools 1.2.<br>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Updated usage for bcftools.<br><br>About<br><br>SAMtools is a popular open-source tool used in next-generation sequence analysis. This primer provides an introduction to SAMtools, and is geared towards those new to next-generation sequence analysis. The primer is also designed to be self-contained and hands-on, meaning that you only need to install SAMtools, and no other tools, and sample data sets are provided. Terms in bold are also explained in the glossary at the end of the document.</p><p>Address of the bookmark: <a href="http://biobits.org/samtools_primer.html" rel="nofollow">http://biobits.org/samtools_primer.html</a></p>]]></description>
	<dc:creator>Jit</dc:creator>
</item>

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