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	<title><![CDATA[BOL: Related items]]></title>
	<link>https://bioinformaticsonline.com/related/14011?offset=1120</link>
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	<guid isPermaLink="true">https://bioinformaticsonline.com/pages/view/21443/a-guide-for-complete-r-beginners-getting-data-into-r</guid>
	<pubDate>Tue, 24 Feb 2015 20:15:08 -0600</pubDate>
	<link>https://bioinformaticsonline.com/pages/view/21443/a-guide-for-complete-r-beginners-getting-data-into-r</link>
	<title><![CDATA[A guide for complete R beginners :- Getting data into R]]></title>
	<description><![CDATA[<p>For a beginner this can be is the hardest part, it is also the most important to get right.</p><p>It is possible to create a vector by typing data directly into R using the combine function &lsquo;c&rsquo;</p><blockquote><p><strong>x </strong></p></blockquote><p>same as</p><blockquote><p><strong>x </strong></p></blockquote><p>creates the vector x with the numbers between 1 and 5.</p><p>You can see what is in an object at any time by typing its name;</p><blockquote><p><strong>x</strong></p></blockquote><p>will produce the output<strong> &lsquo;[1] 1 2 3 4 5&prime;</strong></p><p>Note that names need to be quoted</p><blockquote><p><strong>daysofweek </strong><strong>&larr; c(&lsquo;Monday&rsquo;, &lsquo;Tuesday&rsquo;, &lsquo;Wednesday&rsquo;, &lsquo;Thursday&rsquo;, &lsquo;Friday&rsquo;);</strong></p></blockquote><p>Usually however you want to input from a file. We have touched on the &lsquo;read.table&rsquo; function already.</p><blockquote><p><strong>mydata </strong></p></blockquote><p>Now <strong>mydata</strong> is a data frame with multiple vectors</p><p>each vector can be identified by the default syntax</p><p>#if any of these are typed it will print to screen</p><blockquote><p><strong>mydata$V1 mydata$V2 mydata$V3 </strong></p></blockquote><p>By default the function assumes certain things from the file</p><ul>
<li>The file is a plain text file (there are function to read excel files: <em>not covered here</em>)</li>
<li>columns are separated by any number of tabs or spaces</li>
<li>there is the same number of data points in each column</li>
<li>there is no header row (labels for the columns)</li>
<li>there is no column with names for the rows** [I&rsquo;ll explain].</li>
</ul><p><span style="text-decoration: underline;">If any of these are false, we need to tell that to the function</span></p><p>If it has a header column</p><blockquote><p><strong>mydata <em>header=T also works</em></strong></p></blockquote><p>Note that there is a comma between different parts of the functions arguments</p><p>If there is one less column in the header row, then R assumes that the 1<sup>st</sup> column of data after the header are the row names</p><p>Now the vectors (columns) are identified by their name</p><p>#if any of these are typed it will print to screen</p><blockquote><p><strong>mydata$A mydata$B mydata$C </strong></p></blockquote><p># Summary about the whole data frame</p><blockquote><p><strong>summary(mydata)</strong></p></blockquote><p># Summary information of column A</p><blockquote><p><strong>summary(mydata$A) </strong></p></blockquote><p>We can shortcut having to type the data frame each time by attaching it</p><blockquote><p><strong>attach(mydata)</strong></p></blockquote><p># summary of column B as &lsquo;mydata&rsquo; is attached</p><blockquote><p><strong>summary(B)</strong></p></blockquote><p><span style="text-decoration: underline;">Two other important options for </span><em><span style="text-decoration: underline;">read.table</span></em></p><p>If is is separated only by tabs and has a header</p><blockquote><p><strong>mydata </strong></p></blockquote><p>Really useful if you have spaces in the contents of some columns, so R does not mess up reading the columns . However if the columns or of an uneven length it will tell you.</p><p>If you know that the file has uneven columns</p><blockquote><p><strong>mydata </strong></p></blockquote><p>This causes R to fill empty spaces in a columns with &lsquo;NA&rsquo; .</p><p>The last two examples will still work with our file and give the same result as with only headers=T</p><p><span style="text-decoration: underline;">Graphs</span></p><p>to get an idea of what R is capable of type</p><blockquote><p><strong>demo(graphics)</strong></p></blockquote><p>steps through the examples, and the code is printed to the screen</p><p>We will work with simpler examples that have immediate use to biologists.</p><p>Remember to get more information about the options to a function type &lsquo;?function&rsquo;</p><p><span style="text-decoration: underline;">Histogram of A</span><span style="text-decoration: underline;"></span></p><blockquote><p><strong>hist(mydata$A)</strong></p></blockquote><p>If there was more data we could increase the number of vertical columns with the option, breaks=50 (or another relevant number).</p><blockquote><p><strong>boxplot(mydata)</strong></p></blockquote><p>We can get rid of the need to type the data frame each time by using the <strong>attach</strong> function</p><p># if not already done so</p><blockquote><p><strong>attach(mydata) </strong></p><p><strong>boxplot(mydata$A, mydata$B, name=c(&ldquo;Value A&rdquo;, &ldquo;Value B&rdquo;) , ylab=&ldquo;Count of Something&rdquo;)</strong></p></blockquote><p>same as</p><blockquote><p><strong>boxplot(A, B, name=c(&ldquo;Value A&rdquo;, &ldquo;Value B&rdquo;) , ylab=&ldquo;Count of Something&rdquo;)</strong></p></blockquote><p><span style="text-decoration: underline;">Scatter plot</span></p><p># if not already done so</p><blockquote><p><strong>attach(mydata) </strong></p><p><strong>plot(A,B) # or plot(mydata$A, mydata$B)</strong></p></blockquote><p><strong><span style="text-decoration: underline;">SAVING an image</span></strong></p><p>Windows users (Rgui) RIGHT click on image and select which you want.</p><p><span style="text-decoration: underline;">These instructions work for everyone.</span></p><p>You need to create a new device of the type of file you need, then send the data to that device</p><p>to save as a png file (easy to load into the likes of powerpoint, also great for web applications.</p><blockquote><p><strong>png(&lsquo;filename&rsquo;) </strong></p><p><strong>boxplot(A, B, name=c(&ldquo;Value A&rdquo;, &ldquo;Value B&rdquo;) , ylab=&ldquo;Count of Something&rdquo;)</strong></p></blockquote><p>or to save as a pdf</p><blockquote><p><strong>pdf(&lsquo;filename&rsquo;) </strong></p><p><strong>boxplot(A, B, name=c(&ldquo;Value A&rdquo;, &ldquo;Value B&rdquo;) , ylab=&ldquo;Count of Something&rdquo;)</strong></p></blockquote><p><span style="text-decoration: underline;">Note</span></p><ul>
<li>Nothing will appear on screen, the output is going to the file</li>
<li>Also it may not be saved immediately but will once the device (or R) is turned quit.</li>
</ul><p>To quit R type</p><p><strong>q() # </strong>If you save your session, next time you start R, you will have your data preloaded.</p><p>Or if you want to remain in R</p><blockquote><pre><strong>dev.off() #</strong>turns of the png (or pdf etc) device, thus forces the data to save</pre></blockquote>]]></description>
	<dc:creator>Archana Malhotra</dc:creator>
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  <guid isPermaLink='true'>https://bioinformaticsonline.com/researchlabs/view/42958/claus-peter-stelzer-lab</guid>
  <pubDate>Mon, 15 Mar 2021 15:24:41 -0500</pubDate>
  <link></link>
  <title><![CDATA[Claus-Peter Stelzer Lab]]></title>
  <description><![CDATA[
<p>Interested in various topics at the intersection of ecology and evolution. In my research I use rotifers as model organisms for experimental studies at the individual and population level. Rotifers are ideally suited for this, because populations of thousands can be kept in small containers in the lab, while single individuals can still be handled conveniently. </p>

<p>More at https://www.uibk.ac.at/limno/personnel/stelzer/index.html.en#research</p>
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  <guid isPermaLink='true'>https://bioinformaticsonline.com/opportunity/view/21539/research-associate-at-central-potato-research-institute-cpri-shimla-himachal-pradesh</guid>
  <pubDate>Wed, 11 Mar 2015 03:07:37 -0500</pubDate>
  <link></link>
  <title><![CDATA[RESEARCH ASSOCIATE at Central Potato Research Institute (CPRI) - Shimla, Himachal Pradesh]]></title>
  <description><![CDATA[
<p>One post of Research Associate for Project Implementation Unit in the time bound project “XII Plan -–Centre of Agricultural Bio-informatics(CABIN)” are to be filled on purely contractual basis which will be co-terminus with the project as per the details given as under : </p>

<p>No of post : 01 <br />Essential qualifications: i) Ph. D degree in Bioinformatics/computers/Bio-technology. OR ii) Master’s Degree in Bioinformatics/computers/Bio-technology with 1st division or 60% marks or equivalent overall grade point average with at least two years of research experience as evidenced from fellowship/Associateship/training/other engagements. <br />Desirable qualifications: i) Working Knowledge and Published Research papers in Bio-informatics. <br />Monthly emoluments : Rs. 23,000/- + HRA . for M.Sc degree holder Rs. 24,000/- + HRA for Ph.D degree holder <br />Maximum Age limit : Research Associate – Males- 40 years &amp; Women 45 years. <br />SELECTION PROCEDURE FOR CENTRAL POTATO RESEARCH INSTITUTE (CPRI) – RESEARCH ASSOCIATE POST: </p>

<p>Written Test on 20/03/2015. <br />Shortlisted candidates will undertake face to face interview. <br />Dates are yet to be announced for the final selection <br />WALK-IN PROCEDURE FOR RESEARCH ASSOCIATE VACANCY IN CENTRAL POTATO RESEARCH INSTITUTE (CPRI): </p>

<p>Interested/eligible candidates should submit their application along with the attested copies of educational qualification (provisional degree of Masters and Ph.D is mandatory )/experience certificates and one passport size photograph to the Asstt. Admn. Officer(E-I), CPRI, Shimla-171001 at 9.30 AM on the date of interview. The candidates appearing for interview must bring original certificate with them and only those candidates possessing essential qualification as per advertisement will be interviewed. The Director, CPRI, Shimla reserves the right either to fill up the post or cancel the interview without assigning any reasons thereof. Application form is available in the website ( website: http//cpri.ernet.in). No TA/DA will be given by the Institute to the candidates. The Institute is located at Bemloe which is about 2 Kms from Main Bus Stand(Old)/3 Kms. from the Railway Station and about 5 Kms. from ISBT (Tutikandi).</p>
]]></description>
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	<guid isPermaLink="true">https://bioinformaticsonline.com/videolist/watch/4960/genome-epigenome-new-understanding-of-the-pathogens-in-your-food</guid>
	<pubDate>Fri, 27 Sep 2013 11:30:45 -0500</pubDate>
	<link>https://bioinformaticsonline.com/videolist/watch/4960/genome-epigenome-new-understanding-of-the-pathogens-in-your-food</link>
	<title><![CDATA[Genome + Epigenome = New Understanding of the Pathogens in Your Food]]></title>
	<description><![CDATA[<iframe width="" height="" src="https://www.youtube-nocookie.com/embed/hGtHs_C1BFA" frameborder="0" allowfullscreen></iframe>UC Davis's Bart Weimer describes foodborne pathogens and their proclivity for rapid genome rearrangement. The 100K Pathogen Genome Project he leads is using PacBio long-read sequencing to close genomes and analyze methylation; Weimer reports that his team has already discovered new epigenetic modifications in Salmonella and Listeria with the technology. www.pacb.com/microbe]]></description>
	
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  <guid isPermaLink='true'>https://bioinformaticsonline.com/opportunity/view/21625/agricul-agricultural-scientists-recruitment-board-tural-scientists-recruitment-board-new-delhi-110-012</guid>
  <pubDate>Wed, 11 Mar 2015 09:18:37 -0500</pubDate>
  <link></link>
  <title><![CDATA[AGRICUL AGRICULTURAL SCIENTISTS RECRUITMENT BOARD TURAL SCIENTISTS RECRUITMENT BOARD NEW DELHI-110 012]]></title>
  <description><![CDATA[
<p>ADVERTISEMENT NO. 01/2015</p>

<p>PRINCIPAL SCIENTIST Pay Band: Minimum pay of `43,000 in the PB-4 of `37400-67000/- + RGP of `10,000/-.</p>

<p>Age: The candidates must not have attained the age of 52 years as on 24.03.2015. There shall be no age limit for the Council’s employees.</p>

<p>ICAR-NATIONAL INSTITUTE OF BIOTIC STRESS MANAGEMENT, RAIPUR (CHHATTISGARH)</p>

<p>57. Principal Scientist (Agricultural Entomology) (Two post)</p>

<p>Qualifications Essential:</p>

<p>(i) Doctoral degree in Agricultural Entomology including relevant basic sciences.</p>

<p>(ii) 10 years experience in the relevant subject out of which at least 8 years should be as Scientist/ Lecturer/Extension Specialist or in an equivalent position in the Pay Band- 3 of `15600-39100 with Grade Pay of `5400/`6000/`7000/`8000 and 2 years as a Senior Scientist or in an equivalent position in the Pay Band- 4 of ` 37400-67000 with Grade Pay of ` 8700/ ` 9000.</p>

<p>(iii) The candidate should have made contribution to research/teaching/extension education as evidenced by published work/innovations and impact.</p>

<p>Desirable:</p>

<p>(i) Experience of using frontiers research tools in management of insect pests of crop plants.</p>

<p>(ii) Evidence of contributions to relevant field through publications/ patents/citation index to suggest a vision/perspective in biotic stress research.</p>

<p>61. Principal Scientist (Bioinformatics) (One post)</p>

<p>Qualifications Essential:</p>

<p>(i) Doctoral degree in Bioinformatics including relevant basic sciences. (ii) &amp; (iii) As in item no. 57 above.</p>

<p>Desirable:</p>

<p>(i) Experience of using bioinformatics for advancement of knowledge and for research on biotic stress management.</p>

<p>(ii) Evidence of contributions to relevant field through publications/patents/citation index to suggest a vision/perspective in biotic stress research.</p>

<p>http://asrb.org.in/administrator/uploads_dir/1424859407english.pdf</p>
]]></description>
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	<guid isPermaLink="true">https://bioinformaticsonline.com/file/view/18653/genetic-code-amino-acid</guid>
	<pubDate>Sun, 26 Oct 2014 07:45:58 -0500</pubDate>
	<link>https://bioinformaticsonline.com/file/view/18653/genetic-code-amino-acid</link>
	<title><![CDATA[Genetic code - Amino Acid]]></title>
	<description><![CDATA[<p>The genetic code consists of 64 triplets of nucleotides. These triplets are called codons.With three exceptions, each codon encodes for one of the 20 amino acids used in the synthesis of proteins. That produces some redundancy in the code: most of the amino acids being encoded by more than one codon.</p><p>The image summarise all in one.</p><p>More at http://users.rcn.com/jkimball.ma.ultranet/BiologyPages/C/Codons.html</p>]]></description>
	<dc:creator>Poonam Mahapatra</dc:creator>
	<enclosure url="https://bioinformaticsonline.com/file/download/18653" length="226605" type="image/jpeg" />
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  <guid isPermaLink='true'>https://bioinformaticsonline.com/opportunity/view/21680/research-associate-at-national-research-centre-on-plant-biotechnology-new-delhi</guid>
  <pubDate>Mon, 16 Mar 2015 03:22:26 -0500</pubDate>
  <link></link>
  <title><![CDATA[Research Associate at National Research Centre on Plant Biotechnology New Delhi]]></title>
  <description><![CDATA[
<p>Walk-in interview will be held on 24-03-2015 at 10:00 AM at NRCPB, New Delhi for filling Research Associate and Senior Research Fellow positions as mentioned below. The positions are temporary and are initially offered for a period of one year. Details such as emoluments, qualifications, application format etc., are given below. Desirous candidates should report for interview latest by 10:30 AM with the application in the prescribed format, copies and originals of certificates, thesis and documents. No TA/DA will be provided for attending the interview.</p>

<p>ICAR-NPTC: Fibre development in flax/linseed.</p>

<p>(Job # 1) Research Associate (one) (Bioinformatics)</p>

<p>Rs.24000+ 30% HRA) for Ph.D. and for M. Sc Rs.23000/‐ (+ 30% HRA)</p>

<p>Ph.D. Degree in Bioinformatics/Molecular Biology/Biotechnology/ Genetics/allied sciences; or M. Sc in Bioinformatics/ Biotechnology/Life Sciences/ allied sciences with 1st division or 60% marks or equivalent overall grade point average with at least two years of research experience as evidenced from Fellowship/ Associate ship. 2 years research experience in bioinformatic data analysis/molecular biology techniques, and high throughput DNA/RNA sequencing, and transcriptome data analysis. Research paper with IF&gt;1 will be desirable</p>

<p>ICAR-NPTC: Shade avoidance/low-light tolerance in rice.</p>

<p>General Terms &amp; Conditions applicable to all the positions: <br />Age Limit: 35 years max. (5 years relaxation for SC/ST/OBC and woman candidates as per ICAR rules). <br />The positions are purely temporary, on a contractual basis and are initially offered for one year. <br />Originals must be shown for verification. 7. Research experience (Experience certificate from previous employer to be attached): I hereby declare that the information provided above is true to the best of my knowledge. Date: Signature</p>

<p>Advertisement:</p>

<p>www.nrcpb.org/sites/default/files/ICAR-NPTC%20DBT%20RA%20SRF%20interview%2024th%20March.pdf</p>
]]></description>
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	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/33221/genome-annotation-transfer-utility-gatu</guid>
	<pubDate>Mon, 29 May 2017 05:54:53 -0500</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/33221/genome-annotation-transfer-utility-gatu</link>
	<title><![CDATA[Genome Annotation Transfer Utility (GATU)]]></title>
	<description><![CDATA[<p>Genome Annotation Transfer Utility (GATU) was designed to facilitate quick, efficient annotation of similar genomes using genomes that have already been annotated. For example, whenever a new strain of SARS coronavirus is sequenced, it is possible, using GATU, to automatically annotate the new strain using a previously-annotated strain of SARS CoV. This saves researchers from tedious manual annotation of these sequences.</p>
<p>The program utilizes tBLASTn and BLASTn algorithms to map genes from the reference genome (the annotated strain) to the new sequence (the unannotated strain). The goal is to annotate the majority of the new genome&rsquo;s genes in a single step. ORFs present in the target genome and absent from the reference genome are also identified; these ORFs can be further analyzed using BLAST, VGO and BBB. Afterwards, they can either be accepted for/rejected from annotation. GATU can handle multiple-exon genes as well as mature peptides. Although it was designed for use with viral genomes, GATU can also be used to help annotate larger genomes (ie. bacterial genomes).</p>
<p>The output is saved in GenBank, XML, or EMBL file format.</p><p>Address of the bookmark: <a href="https://virology.uvic.ca/help/tool-help/help-books/genome-annotation-transfer-utility-gatu-documentation/" rel="nofollow">https://virology.uvic.ca/help/tool-help/help-books/genome-annotation-transfer-utility-gatu-documentation/</a></p>]]></description>
	<dc:creator>Jit</dc:creator>
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  <guid isPermaLink='true'>https://bioinformaticsonline.com/opportunity/view/21893/postdoctoral-fellowship-in-bioinformatics-and-evolutionary-genomics</guid>
  <pubDate>Wed, 01 Apr 2015 21:36:42 -0500</pubDate>
  <link></link>
  <title><![CDATA[Postdoctoral Fellowship in Bioinformatics and Evolutionary Genomics]]></title>
  <description><![CDATA[
<p>Postdoctoral Fellowship in Bioinformatics and Evolutionary Genomics<br />Organization<br />National Human Genome Research Institute, National Institutes of Health<br />http://genome.gov/Staff/Baxevanis<br />Job Location<br />Bethesda, MD<br />Job Description</p>

<p>A postdoctoral training position is currently available in the Computational and Statistical Genomics Branch (CSGB) of the National Human Genome Research Institute (NHGRI). The position is located in the laboratory of Andy Baxevanis, Ph.D., whose research group uses comparative genomics approaches to better-understand the molecular innovations that drove the surge of diversity in early animal evolution. The overarching theme of Dr. Baxevanis’ research program is focused on how non-traditional animal models convey critical insights into human disease research.</p>

<p>Candidates should have or be close to obtaining a Ph.D. or equivalent degree in bioinformatics, computational biology, computer science, molecular biology, or a closely related field. Candidates with a background in evolutionary biology are particularly encouraged to apply. Programming skills and experience in the application of computational methods to genomic data are highly desirable. Applicants must possess good communication skills and be fluent in both spoken and written English. The ability to learn how to use new software and quickly become expert in its use, critical thinking, problem-solving abilities, and the ability to work semi-independently are required.<br />How to Apply</p>

<p>Interested applicants should submit a curriculum vitae, a detailed letter of interest, and the names of three potential referees to Dr. Baxevanis at andy@mail.nih.gov.<br />About Our Organization</p>

<p>The NIH Intramural Research Program is on the Bethesda, Maryland campus and offers a wide array of training opportunities for scientists early in their careers. The funding for this position is stable and offers the trainee wide latitude in the design and pursuit of their research project. The successful candidate will have access to NHGRI’s established and robust bioinformatics infrastructure, as well as resources made available through NIH’s Center for Information Technology (CIT) and the National Center for Biotechnology Information (NCBI).</p>

<p>For more information on CSGB and NHGRI’s Intramural Research Program, please see http://genome.gov/DIR/.</p>
]]></description>
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	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/34475/oxford-nanopore-sequencing-hybrid-error-correction-and-de-novo-assembly-of-a-eukaryotic-genome</guid>
	<pubDate>Wed, 29 Nov 2017 05:08:53 -0600</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/34475/oxford-nanopore-sequencing-hybrid-error-correction-and-de-novo-assembly-of-a-eukaryotic-genome</link>
	<title><![CDATA[Oxford Nanopore Sequencing, Hybrid Error Correction, and de novo Assembly of a Eukaryotic Genome]]></title>
	<description><![CDATA[<p><span>Monitoring the progress of DNA molecules through a membrane pore has been postulated as a method for sequencing DNA for several decades. Recently, a nanopore-based sequencing instrument, the Oxford Nanopore MinION, has become available that we used for sequencing the S. cerevisiae genome. To make use of these data, we developed a novel open-source hybrid error correction algorithm Nanocorr (</span><a href="https://github.com/jgurtowski/nanocorr">https://github.com/jgurtowski/nanocorr</a><span>) specifically for Oxford Nanopore reads, as existing packages were incapable of assembling the long read lengths (5-50kbp) at such high error rate (between ~5 and 40% error). With this new method we were able to perform a hybrid error correction of the nanopore reads using complementary MiSeq data and produce a de novo assembly that is highly contiguous and accurate: the contig N50 length is more than ten-times greater than an Illumina-only assembly (678kb versus 59.9kbp), and has greater than 99.88% consensus identity when compared to the reference. Furthermore, the assembly with the long nanopore reads presents a much more complete representation of the features of the genome and correctly assembles gene cassettes, rRNAs, transposable elements, and other genomic features that were almost entirely absent in the Illumina-only assembly.</span></p><p>Address of the bookmark: <a href="http://schatzlab.cshl.edu/data/nanocorr/" rel="nofollow">http://schatzlab.cshl.edu/data/nanocorr/</a></p>]]></description>
	<dc:creator>Jit</dc:creator>
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