• Inchworm assembles the RNA-seq data into the unique sequences of transcripts, often generating full-length transcripts for a dominant isoform, but then reports just the unique portions of alternatively spliced transcripts.

• Chrysalis clusters the Inchworm contigs into clusters and constructs complete de Bruijn graphs for each cluster. Each cluster represents the full transcriptonal complexity for a given gene (or sets of genes that share sequences in common). Chrysalis then partitions the full read set among these disjoint graphs.

• Butterfly then processes the individual graphs in parallel, tracing the paths that reads and pairs of reads take within the graph, ultimately reporting full-length transcripts for alternatively spliced isoforms, and teasing apart transcripts that corresponds to paralogous genes.

More at https://github.com/trinityrnaseq/trinityrnaseq/wiki

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Download Trinity here.

Build Trinity by typing 'make' in the base installation directory.

Assemble RNA-Seq data like so:

 Trinity --seqType fq --left reads_1.fq --right reads_2.fq --CPU 6 --max_memory 20G 

Find assembled transcripts as: 'trinity_out_dir/Trinity.fasta'

Address of the bookmark: https://github.com/trinityrnaseq/trinityrnaseq/wiki

Eligibility

Interested applicants must

Have around 10 years of experience as independent investigator, and can be of any age or nationality
Have Sponsor(s) at not-for-profit Host Institution(s) in India, who is willing to extend the desired commitment and resources for program implementation
Provisions

The 5 year Fellowship provides

Generous personal support for the Fellow
Salary support for personnel, which may include Assistant Professors
Large quantum of funds for equipment, animals and consumables
Funds to attend scientific gatherings, for collaborative visits and to organize meetings
Overheads for the Host Institution
Process

A joint online application is invited from the Applicant and the Sponsor(s). The details of the scheme and the funding mechanism are available on the website at http://wellcomedbt.org/fellowshiptype/margdarshi-fellowships.

Application form can be accessed at https://fellowships.wellcomedbt.org/Login.aspx

Sponsored applications due by 2 May 2016

Send your inquiries to margdarshi@wellcomedbt.org

More at http://www.atgc.org/PhyloGrapher/PhyloGrapher_Welcome.html

Address of the bookmark: http://www.atgc.org/PhyloGrapher/PhyloGrapher_Welcome.html

For students interested in frontier research at the interface of biology, computation, physics and applied mathematics

IMSc is a leader in India in fundamental research in theoretical physics, mathematics and theoretical computer science, with several members actively pursuing research in interdisciplinary areas including computational biology. In 2013 IMSc started a unique Ph.D. programme in this subject, training students to apply cutting-edge computational and mathematical techniques to problems in modern biology, in collaboration with leading biology departments and institutions in India and abroad.
IMSc is an autonomous national research institute under the Department of Atomic Energy, Government of India, and a constituent institution of the Homi Bhabha National Institute (HBNI), Mumbai (a deemed university). Ph.D. degrees will be awarded by HBNI.
STRUCTURE OF PROGRAMME
Before embarking on their research, students have three semesters of coursework, which consists of seven core courses, to be carried out at IMSc; elective courses, which may be taken at IMSc or at other institutions by mutual consent; and lab rotations, at collaborating labs in other institutions. The core coursework covers essentials of modern biology, essential techniques from physics, mathematics, statistics and computer science, physics of proteins and biomolecules, biological sequence analysis and algorithms, and systems biology. Elective coursework covers various topics in greater depth. Following the coursework and a comprehensive examination, students will embark on research leading to a Ph.D. degree.
Selected candidates will be research fellows at IMSc and will receive fellowships, housing or house rent allowance, and contingency grants.

More at http://www.imsc.res.in/graduate_programme_0

Title : “Short-Term Research Fellowship”

Qualification : Candidates having M.Sc./M.Tech degree (with minimum of 60% marks overall) or equivalent in Bioinformatics/Biotechnology or any other related field, are eligible to apply. Candidate having prior experience in the area of next-generation sequencing data analysis, bioinformatics, molecular analysis of plant genes, and structural data analysis will be preferred.

No.of Post : 01
How to apply

Application should sent to Dr. Gitanjali Yadav, Staff Scientist-IV, National Institute of Plant Genome Research (NIPGR), Aruna Asaf Ali Marg, P.O. Box NO. 10531, New Delhi - 110067 on or before 26th April 2016

More at http://www.nipgr.res.in/careers/vacancies_latest.php#

DISCOVAR can call variants on a region by region basis, potentially tiling an entire large genome. DISCOVAR variant calling is under active development and transitioning to VCF.

DISCOVAR de novo can generate de novo assemblies for both large and small genomes. It currently does not call variants.

More at https://www.broadinstitute.org/software/discovar/blog/?page_id=14

Address of the bookmark: https://www.broadinstitute.org/software/discovar/blog/

Address of the bookmark: http://busco.ezlab.org/

More at http://mrfast.sourceforge.net/manual.html

Address of the bookmark: http://mrfast.sourceforge.net/manual.html

Experience Required: 3-5 years of experience
No of Positions : Multiple
Qualifications: Candidates with minimum qualification as M.Sc Bioinformatics with 3-5 years of experience in Life sciences R&D or Pharma Industry.
Ph.D candidates with research experience in Bioinformatics with publications in International journal and minimum 2 years of industry experience in clinical genomics will be preferred for this position.

Requirement:

1. Must have basic understanding of molecular biology and Genomics.
2. Experience in application development or must have expertise in programming using either of Perl/Python.
3. Experience in statistical programming using R/Bioconductor/Matlab.
4. Strong concept in statistical and mathematical modelling.
5. Experience in designing and developing the bioinformatics pipeline.
6. Must have minimum 2+ years of hands on experience in NSG data analysis such as RNA-Seq,Exome-Seq ,Chip-Seq and downstream analysis.
7. Knowledge in WGS ,WES, Targeted re-sequencing,GWAS and population genomics will be preferred.
8. Must have experience working on opensource software/Framework and commercial software for NGS data analysis and reporting.
9. Should be aware of handling big data and guiding team members on multiple projects simultaneously.
More at http://www.ocimumbio.com/careers1/
10. Should have experience coordinating with different groups of clinical research scientist for various project requirements.
11. Ability to work as team as well as independently with minimal support.

More at http://www.bioinf.uni-leipzig.de/Software/segemehl/

Manual http://www.bioinf.uni-leipzig.de/Software/segemehl/segemehl_manual_0_1_7.pdf

Address of the bookmark: http://hoffmann.bioinf.uni-leipzig.de/LIFE/segemehl.html