The purpose of the hive plot is to establish a new baseline for visualization of large networks — a method that is both general and tunable and useful as a starting point in visually exploring network structure.

More at http://www.hiveplot.com/

Address of the bookmark: http://www.hiveplot.com/

http://www.bx.psu.edu/~ratan/

Address of the bookmark: http://www.bx.psu.edu/miller_lab/

Using the reference sequence dictionary (*.dict), it also creates some empty BAM files if no sam record was found for a chromosome. A pair of 'mock' SAM-Records can also be added to those empty BAMs to avoid some tools (like samtools) to crash.

Usage

java -jar splitbam.jar -p OUT/__CHROM__/__CHROM__.bam -R ref.fasta (bam|sam|stdin)

Options

• -h help; This screen.
• -R (indexed reference file) REQUIRED.
• -u (unmapped chromosome name): default:Unmapped
• -e | --empty : generate EMPTY bams for chromosome having no read mapped
• -m | --mock : if option '-e', add a mock pair of sam records to the empty bam
• -p (output file/bam pattern) REQUIRED. MUST contain __CHROM__ and end with .bam
• -s assume input is sorted.
• -x | --index create index.
• -t | --tmp (dir) tmp file directory
• -G (file) chrom-group file (see below)

Address of the bookmark: https://code.google.com/archive/p/jvarkit/wikis/SplitBam.wiki

To use CLgenomics, individual genome data (genome sequences + annotation details) are compiled and saved in a specially formatted file called CLG (ChunLab Genomics). Each CLG file corresponds with one bacterial genome. If multiple genomes are being considered and compared, multiple CLG files are needed. ChunLab offers >40,000 CLG files of publicly available Bacterial and Archaeal genomes.

Address of the bookmark: https://chunlab.wordpress.com/clgenomics-software/

https://academic.oup.com/bioinformatics/article/32/4/605/1744462/MaxBin-2-0-an-automated-binning-algorithm-to

The most recent version of MaxBin is 2.2, which supports the analysis of coassemblies of multiple samples. It is available at this JBEI downloads sites as well as MaxBin and MaxBin 2.0 sourceforge sites.

Address of the bookmark: http://downloads.jbei.org/data/microbial_communities/MaxBin/MaxBin.html

GroopM is largely parameter-free. Use: groopm -h for more info.

For installation and usage instructions see : http://ecogenomics.github.io/GroopM/

Address of the bookmark: https://github.com/ecogenomics/GroopM

In C, "sequence statements" are imperatives. The "selection" is the "if then else" statement, and the iteration is satisfied by a number of statements, such as the "while," " do," and the "for," while the case-type statement is satisfied by the "switch" statement.

Pseudocode is an artificial and informal language that helps programmers develop algorithms. Pseudocode is a "text-based" detail (algorithmic) design tool.

The rules of Pseudocode are reasonably straightforward. All statements showing "dependency" are to be indented. These include while, do, for, if, switch. Examples below will illustrate this notion.

GUIDE TO PSEUDOCODE LEVEL OF DETAIL: Given record/file descriptions, pseudocode should be created in sufficient detail so as to directly support the programming effort. It is the purpose of pseudocode to elaborate on the algorithmic detail and not just cite an abstraction.

Examples:

1.

If student's grade is greater than or equal to 60
    Print "passed"
else
    Print "failed"  
endif

2.

  
Set total to zero
Set grade counter to one
While grade counter is less than or equal to ten
    Input the next grade
    Add the grade into the total
endwhile 
Set the class average to the total divided by ten
Print the class average.

3.

Initialize total to zero
Initialize counter to zero
Input the first grade
while the user has not as yet entered the sentinel
   add this grade into the running total 
   add one to the grade counter  
   input the next grade (possibly the sentinel)
endwhile

if the counter is not equal to zero
   set the average to the total divided by the counter
   print the average  
else
   print 'no grades were entered' 
endif 

4.

initialize passes to zero
initialize failures to zero
initialize student to one
while student counter is less than or equal to ten
    input the next exam result  
    if the student passed

add one to passes else add one to failures add one to student counter endif endwhile print the number of passes print the number of failures if eight or more students passed print "raise tuition" endif

5.

Larger example:  

NOTE:  NEVER ANY DATA DECLARATIONS IN PSEUDOCODE

Print out appropriate heading and make it pretty
While not EOF do:
     Scan over blanks and white space until a char is found 
	(get first character on the line)
     set can't-be-ascending-flag to 0
     set consec cntr to 1
     set ascending cntr to 1
     putchar first char of string to screen
     set read character to hold character
     While next character read != blanks and white space
          putchar out on screen
          if new char = hold char + 1
               add 1 to consec cntr
               set hold char = new char
               continue
          endif
          if new char >= hold char 
               if consec cntr < 3 
                    set consec cntr to 1
               endif
               set hold char = new char
               continue
          endif
          if new char < hold char
               if consec cntr < 3
                    set consec cntr to 1
               endif
               set hold char = new char
               set can't be ascending flag to 1
               continue
           endif
     end while
     if consec cntr >= 3 
          printf (Appropriate message 1 and skip a line)
          add 1 to consec total
     endif
     if  can't be ascending flag = 0
          printf (Appropriate message 2 and skip a line)
          add 1 to ascending total
     else
          printf (Sorry message and skip a line)
          add 1 to sorry total
     endif
end While
Print out totals:  Number of consecs, ascendings, and sorries.
Stop

Some Keywords That Should be Used And Additional Points

For looping and selection, The keywords that are to be used include Do While...EndDo; Do Until...Enddo; While .... Endwhile is acceptable. Also, Loop .... endloop is also VERY good and is language independent. Case...EndCase; If...Endif; Call ... with (parameters); Call; Return ....; Return; When;

Always use scope terminators for loops and iteration.

As verbs, use the words Generate, Compute, Process, etc. Words such as set, reset, increment, compute, calculate, add, sum, multiply, ... print, display, input, output, edit, test , etc. with careful indentation tend to foster desirable pseudocode. Also, using words such as Set and Initialize, when assigning values to variables is also desirable.

More on Formatting and Conventions in Pseudocoding

• INDENTATION in pseudocode should be identical to its implementation in a programming language. Try to indent at least four spaces.
• As noted above, the pseudocode entries are to be cryptic, AND SHOULD NOT BE PROSE. NO SENTENCES.
• No flower boxes (discussed ahead) in your pseudocode.
• Do not include data declarations in your pseudocode.
• But do cite variables that are initialized as part of their declarations. E.g. "initialize count to zero" is a good entry.

  ---

  Function Calls, Function Documentation, and Pseudocode
• Calls to Functions should appear as:
• Returns in functions should appear as:
• Function headers should appear as:
• Note that in C, arguments and parameters such as "fieldn" could be written: "pointer to fieldn ...."
• Functions called with addresses should be written as:
• Function headers containing pointers should be indicated as:
• Returns in functions where a pointer is returned:
• It would not hurt the appearance of your pseudocode to draw a line or make your function header line "bold" in your pseudocode. Try to set off your functions.
• Try to use scope terminators in your pseudocode and source code too. It really hels the readability of the text.

  ---

  Source Code
• EVERY function should have a flowerbox PRECEDING IT. This flower box is to include the functions name, the main purpose of the function, parameters it is expecting (number and type), and the type of the data it returns. All of these listed items are to be on separate lines with spaces in between each explanatory item.
• FORMAT of flowerbox should be

   

  	 ********************************************************
  	 Function:   ( cryptic text describing single function
  		     ....... (indented like this) 	
  		     .......
  	 Calls:      Start listing functions "this" function calls
  		     Show these functions:  one per line, indented
  
  	 Called by:  List of functions that calls "this" function
  		     Show these functions:  one per line, indented.
  
  	 Input Parameters:  list, if appropriate; else None
  	 
  	 Returns:    List, if appropriate.
  	 ****************************************************************
  

• INDENTATION is critically important in Source Code. Follow standard examples given in class. If in doubt, ASK. Always indent statements within IFs, FOR loops, WILLE loops, SWITCH statements, etc. a consistent number of spaces, such as four. Alternatively, use the tab key. One or two spaces is insufficient.
• Use scope terminators at the end of if statements, for statements, while statements, and at the end of functions. It will make your program much more readable.

  SPELLING ERRORS ARE NOT ACCEPTABLE

Genome sequences of rare, uncultured bacteria obtained by differential coverage binning of multiple metagenomes

Mads Albertsen, Philip Hugenholtz, Adam Skarshewski, Gene W. Tyson, Kåre L. Nielsen and Per .H. Nielsen

Nature Biotechnology 2013, doi: 10.1038/nbt.2579

See the associated online guide for detailed information.

https://github.com/MadsAlbertsen/multi-metagenome

Address of the bookmark: https://github.com/MadsAlbertsen/multi-metagenome

Address of the bookmark: https://github.com/marbl/Krona/wiki