<![CDATA[BOL: Related items]]> https://bioinformaticsonline.com/related/24264?offset=1090 https://bioinformaticsonline.com/opportunity/view/39826/data-scientist-mwd Wed, 07 Aug 2019 03:19:46 -0500 <![CDATA[Data Scientist (m/w/d)]]> https://uk-erlangen.concludis.de/prj/shw/b8b26f24186191c7af8a25e2cc6115ca_0/27008/Data_Scientist_m_w_d.htm?b=0

Deadline: 30.09.2019

Requirement:
Microsoft SQL Server: Administration, Transact-SQL
Machine Learning
Know Deutsch und Englisch langauge

]]> https://bioinformaticsonline.com/news/view/39865/blast-nr-version-5-database-nr-v5 Fri, 23 Aug 2019 11:35:35 -0500 https://bioinformaticsonline.com/news/view/39865/blast-nr-version-5-database-nr-v5 <![CDATA[BLAST nr version 5 database, (nr_v5)]]> NCBI have made changes the nr version 5 database, (nr_v5), to facilitate better search results and improved performance by reducing the number of redundant titles in the nr_v5 database used by webBLAST, which is also available for BLAST+ users.

The changes in nr preserve the taxonomic diversity of the entries in the database while reducing the number of titles for identical sequences. GenPept accessions are still accessible via www.ncbi.nlm.nih.gov/protein/$GENBANK_ACCESSION or the IPG website https://www.ncbi.nlm.nih.gov/ipg/.

The "Identical Proteins" link in the alignments section of the webBLAST results takes you to a full list of all accessions associated with a sequence.

For BLAST+ users downloading nr_v5: the database is now approximately 50% smaller, resulting in faster downloads and BLAST searches, and smaller disk space requirements. The database is downloadable at:  ftp://ftp.ncbi.nlm.nih.gov/blast/db/v5/

For BLAST+ there is a cleanup script to help you manage the transition to this smaller database. The script removes unused database volumes: ftp://ftp.ncbi.nlm.nih.gov/blast/temp/cleanup-blastdb-volumes.py

Here are the new rules on how we keep titles in nr_v5:

1.    We keep all refseq, swissprot, pir and PDB titles.

2.    We keep any GenPept titles with a TAXID that has not already been seen in the record.

3.    We keep at least five GenPept titles regardless of whether the TAXIDS have been seen before or not in this record.

]]> Jit https://bioinformaticsonline.com/news/view/12883/breaking-chromosomes-to-study-cancer Fri, 18 Jul 2014 05:42:09 -0500 https://bioinformaticsonline.com/news/view/12883/breaking-chromosomes-to-study-cancer <![CDATA[Breaking chromosomes to study cancer !!!]]> Chromosomes are present in every cell of our body and they contain the information the body needs to develop and function properly. This information is carried in genes that are arranged along the chromosomes. There are usually 46 chromosomes in every cell. These chromosomes come in pairs, one from our mother and one from our father. The chromosomes can be sorted into 23 pairs by looking at them down a microscope.

Most people who have a balanced translocation have the right amount of chromosome material but it has been rearranged in some way. This may happen if two chromosomes swap pieces (a reciprocal translocation). In other cases two whole chromosomes may become stuck together (a Robertsonian translocation). This page describes what happens when someone has a reciprocal translocation.

Reciprocal chromosomal translocations occur following double-strand breaks (DSBs) in DNA when a section of one chromosome is exchanged with that of another, non-homologous chromosome. These exchanges may produce a dysfunctional fusion gene that disrupts cell growth and survival pathways, such as the translocations seen in leukemia and childhood sarcomas.

Chromosomal translocations have been well studied in cancer cell lines which are associated with two types of cancer, acute myeloid leukemia and Ewing's sarcoma, but determining how they contribute to cancer development is complicated by additional mutations and altered gene expression profiles in these cultured cells. Now, Juan Carlos Ramirez, head of the Viral Vector Facility at the Fundacion Centro Nacional de Investigaciones Cardiovasculares (CNIC) and his colleagues Raul Torres at CNIC and Sandra Rodriguez-Peralez at the Spanish National Cancer Center (CNIO) in Madrid, Spain have used a new genome editing tool, CRISPR-Cas9, to induce chromosomal translocations for the first time in a human cell line and in primary cells. The study's authors conclude by stating that the use of this technology will allow for the clarification of how and why chromosomal translocation occurs, which without doubt will allow new anti-cancer therapeutic strategies to be tackled.

Using RNA-Guided Endonuclease (RGEN) technology or CRISPR/Cas9 genome engineering technology, CNIO and CNIC researchers have shown that it is possible to obtain such chromosomal translocations. The CRISPR-Cas9 system is extremely simple to introduce a cut at the desired locus, easier to design, and cheaper than many other systems. Using the CRISPR-Cas9 system, Ramirez and his colleagues reproduced the translocations observed in Ewing’s Sarcoma (ES) and Acute Myeloid Leukemia (AML) patient cell lines in HEK293 cells and also generated the ES translocation in human mesenchymal stem cells and the AML translocation in umbilical cord blood cells.

By focusing on chromosomal translocation without the confounding characteristics of established cell lines, these new cells lines should help answer the fundamental question of what causes a cell to become cancerous. Ramirez and his team now look forward to modeling other chromosome translocations in a variety of cell types.

Reference:

http://en.wikipedia.org/wiki/Chromosomal_translocation

http://www.nature.com/ncomms/2014/140603/ncomms4964/abs/ncomms4964.html

]]> Jit https://bioinformaticsonline.com/bookmarks/view/43011/deg-50-a-database-of-essential-genes-in-both-prokaryotes-and-eukaryotes Tue, 30 Mar 2021 11:47:28 -0500 https://bioinformaticsonline.com/bookmarks/view/43011/deg-50-a-database-of-essential-genes-in-both-prokaryotes-and-eukaryotes <![CDATA[DEG 5.0: a database of essential genes in both prokaryotes and eukaryotes]]> Essential genes are those indispensable for the survival of an organism, and their functions are therefore considered a foundation of life. Determination of a minimal gene set needed to sustain a life form, a fundamental question in biology, plays a key role in the emerging field, synthetic biology.

DEG is freely available at the website http://tubic.tju.edu.cn/deg or http://www.essentialgene.org.

Address of the bookmark: http://www.essentialgene.org/

]]> Rahul Nayak https://bioinformaticsonline.com/bookmarks/view/43685/chipbase-open-database-for-studying-the-transcription-factor-binding-sites-and-motifs Wed, 29 Dec 2021 05:36:03 -0600 https://bioinformaticsonline.com/bookmarks/view/43685/chipbase-open-database-for-studying-the-transcription-factor-binding-sites-and-motifs <![CDATA[ChIPBase: open database for studying the transcription factor binding sites and motifs]]> ChIPBase v2.0 is an open database for studying the transcription factor binding sites and motifs, and decoding the transcriptional regulatory networks of lncRNAs, miRNAs, other ncRNAs and protein-coding genes from ChIP-seq data. Our database currently contains ~10,200 curated peak datasets derived from ChIP-seq methods in 10 species.

Address of the bookmark: https://rna.sysu.edu.cn/chipbase/

]]> Abhi https://bioinformaticsonline.com/bookmarks/view/3868/next-generation-sequencing-ngs-tutorials Sat, 24 Aug 2013 06:01:37 -0500 https://bioinformaticsonline.com/bookmarks/view/3868/next-generation-sequencing-ngs-tutorials <![CDATA[Next Generation Sequencing (NGS) Tutorials]]> Institute of computational biomedicine, Cornell University provide an NGS workshop tutorial at http://chagall.med.cornell.edu/NGScourse/ 

You can also add your favourite NGS educational material, or workshop tutorial by commenting on this bookmarks for user benefit. 

Understanding the basics of genome sequencing:

Tutorial by Luke Jostins.

http://www.genetic-inference.co.uk/blog/2009/04/basics-sequencing-dna-part-1/

http://www.genetic-inference.co.uk/blog/2009/08/basics-sequencing-dna-part-2/

A window into third-generation sequencing

http://hmg.oxfordjournals.org/content/19/R2/R227.full.pdf

==============================================

NGS data analysis pipelines

  • Detecting and annotating genetic variations using the HugeSeq pipeline  DOI: 10.1038/nbt.2134
  • NARWHAL, a primary analysis pipeline for NGS data http://bioinformatics.oxfordjournals.org/cgi/content/abstract/28/2/284?etoc
  • RseqFlow: Workflows for RNA-Seq data analysis  DOI: 10.1093/bioinformatics/btr441
  • ngs_backbone: a pipeline for read cleaning, mapping and SNP calling using Next Generation Sequence  10.1186/1471-2164-12-285
  • A framework for variation discovery and genotyping using next-generation DNA sequencing data  PubMed: 21478889
  • SNiPlay: a web-based tool for detection, management and analysis of SNPs. Application to grapevine diversity projects  DOI: 10.1186/1471-2105-12-134 Abstract: http://www.biomedcentral.com/1471-2105/12/134/abstract
  • WEP: a high-performance analysis pipeline for whole-exome data http://www.biomedcentral.com/1471-2105/14/S7/S11
  • DDBJ read annotation pipeline: a cloud computing-based pipeline for high-throughput analysis of next-generation sequencing data. http://www.ncbi.nlm.nih.gov/pubmed/23657089
  • GATK: a Toolkit for Genome Analysis http://www.broadinstitute.org/gatk/
  • Metagenomics:http://www.nbic.nl/education/nbic-phd-school/course-schedule/ngsmetagenomics/
  • RNASeq:http://www.nbic.nl/education/nbic-phd-school/course-schedule/ngsrnaseq/
  • Bioinformatics and Seq courses: http://www.isb-sib.ch/training/training-activities-schedule/archive-2013.html
  • Variant Detection (Model organism) Advanced tutorial https://docs.google.com/document/pub?id=1CuKkKylVDb03tnN7RSWl5EUzleetn0ctjmvaidPKLxM
  • Variant Detection Introductory tutorial https://docs.google.com/document/pub?id=1ZRzrjjOCvtAu3m-IKL-rbJ1f4On60dDL_IEwG7oejdI
  • Microbial de novo Assembly for Illumina Data Introductory tutorial https://docs.google.com/document/pub?id=1N3AB9ptISUu4zULqe1kXpVF0BDyGb5f5yzxWSJd_WNM
  • RNAseq Differential Gene Expression Introductory tutorial https://docs.google.com/document/pub?id=1KbTiBHtvHLfPRZ39AY3uriazrINA8TJzgjjwn1zPP7Y

" Please add your favourite NGS link below in comment section for the benefit of bioinformatics community ". 

Address of the bookmark: http://chagall.med.cornell.edu/NGScourse/

]]> Jitendra Narayan https://bioinformaticsonline.com/file/view/4219/brief-biodata-of-dr-pranjal-chandra Wed, 04 Sep 2013 11:14:03 -0500 https://bioinformaticsonline.com/file/view/4219/brief-biodata-of-dr-pranjal-chandra <![CDATA[Brief Biodata Of Dr. Pranjal Chandra]]> Pranjal Chandra PhD https://bioinformaticsonline.com/pages/view/18749/list-of-biotechnology-research-laboratory-university-and-centre-in-india Thu, 30 Oct 2014 12:34:34 -0500 https://bioinformaticsonline.com/pages/view/18749/list-of-biotechnology-research-laboratory-university-and-centre-in-india <![CDATA[List of Biotechnology Research Laboratory, University, and Centre in India]]> DEPARTMENT OF BIOTECHNOLOGY

INDIAN COUNCIL OF MEDICAL RESEARCH (ICMR)

COUNCIL OF SCIENTIFIC & INDUSTRIAL RESEARCH (CSIR)

 

CITYWISE

City Wise Llist of Govt. and Non Govt. Research institutions in India -

Ahmedabad

Allahabad

Bangalore

Barrackpore (W. B.)

· Central Inland Capture Fisheries Research Institute

Bhopal

Bhubaneswar

Calcutta

Chandigarh

Chennai (Madras)

Delhi

Dehradun

Dirang (Arunachal Pradesh)

Durgapur

Eluru (A. P.)

Gandhi Nagar

Goa

Hyderabad

Indore

Jammu

Jhansi (U. P)

Jodhpur (Rajasthan)

Kanpur

Kasaragod

Kharagpur

Kochi

Lucknow

Mathura

Mumbai (Bombay)

Palakkad

Palampur (H.P.)

Peechi

Pilani

Puttur (Karnataka)

Pune

Roorkie (U. P.)

Trivandrum (Thiruvananthapuram)

Varanasi (U. P.)

· Indian Institute of Vegetable Research

 

UNIVERSITIES/COLLEGES UNDER UGC

DST

•  Agharkar Research Institute, Pune

•  Birbal Sahni Institute of Palaeobotany, Lucknow

•  Bose Institute, Kolkata

•  Indian Association for the Cultivation of Science, Kolkata

•  International Advanced Research Centre for Powder Metallurgy and New Materials,
...esr ...Hyderabad

•  Indian Institute of Astrophysics, Bangalore

•  Indian Institute of Geomagnetism, Mumbai

•  Indian Institute of Tropical Meteorology, Pune

•  Jawaharlal Nehru Centre for Advanced Scientific Research, Bangalore

•  National Accreditation Board for Testing & Calibration Laboratories, New Delhi

•  Raman Research Institute, Bangalore

•  S.N. Bose National Centre for Basic Sciences, Kolkata

•  Sreechitra Tirunal Institute for Medical Sciences & Technology, Thiruvananthapuram

•  Technology Information, Forecasting & Assessment Council (TIFAC), New Delhi

•  Vigyan Prasar, New Delhi

 

Indian Council of Agricultural Research

  • National Bureaue of Soil Survey & Land Use Planning (NBSSLUP)
  • Central Research Institute for Jute & Allied Fibres (CRIJAF)
  • National Bureaue of Agriculturally Important Micro-Organisms (NBAIMO
  • Central Research Institute for Dryland Agriculture
]]> Jit https://bioinformaticsonline.com/opportunity/view/42418/scientist-b-bioinformatics-at-aiims-delhi Sun, 20 Dec 2020 04:34:55 -0600 <![CDATA[Scientist-B (Bioinformatics) at AIIMS, Delhi]]> Name of the Project: “Artificial intelligence in Oncology, Harnessing big data and advanced computing to provide personalized diagnosis and treatment for Cancer patients”

Age Limit: 35

How to Apply for the AIIMS Life Science Job:

Interested applicants are asked to send out a detailed CV to Dr Ashok Sharma (aioncoaiims@gmail.com). Laboratory of Chromatin and also Cancer Epigenetics, Department of Biochemistry with the subject line “Application for Scientist-B position for MeitY project” latest by January 01st, 2021.
Complete Information of the year of passing, experience, marks, etc. ought to be mentioned in the CV Incomplete. applications will certainly be rejected Just shortlisted applicants will be called for interview. Chosen candidates will certainly be intimated by email/phone.
No TA/DA will certainly be paid for appearing in the interview.
Note, The institute reserved the right to fill up or not to fill up the post advertised.

Emoluments: Rs. 56,000/- plus 24 percent HRA

Eligibility:
2nd class Master’s Degree with a PhD in a pertinent subject (Bioinformatics) from.a recognized University
1st class Master’s degree in Life Sciences (Bioinformatics) from a recognized university OR.
Bachelor’s Degree in Engineering or-Technology with minimal 60% marks from a recognized University or equivalent.

Desirable Qualifications:
Experience in Bioinformatics/NGS data. Analysis/System Biology/Computer Science/ statistics with experience in Machine learning/Al project.
Experience of Deep learning applications in biological data ( image/text).
Proficient in Rf Python machine learning libraries.
Prior experience in the cancer-related project (ML-based) will be advantageous.
Experience with PyTorch/TensorFlow will certainly be very desirable.
Applicant should have strong scientific writing as well as. verbal abilities.
Papers in sci-indexed journals demonstrating ML skill sets.
Database handling will certainly be plus yet not required.

More detail at https://www.aiims.edu/images/pdf/recruitment/advertisement/biochem-16-12-20.pdf

]]> https://bioinformaticsonline.com/bookmarks/view/38804/grabb-selective-assembly-of-genomic-regions-a-new-niche-for-genomic-research Sat, 26 Jan 2019 18:58:16 -0600 https://bioinformaticsonline.com/bookmarks/view/38804/grabb-selective-assembly-of-genomic-regions-a-new-niche-for-genomic-research <![CDATA[GRAbB: Selective Assembly of Genomic Regions, a New Niche for Genomic Research]]> GRAbB is shown to be more efficient than MITObim in terms of speed, memory and disk usage. The other functionalities (handling multiple targets simultaneously and extracting homologous regions) of the new program are not matched by other programs. The program is available with explanatory documentation at https://github.com/b-brankovics/grabb. GRAbB has been tested on Ubuntu (12.04 and 14.04), Fedora (23), CentOS (7.1.1503) and Mac OS X (10.7). Furthermore, GRAbB is available as a docker repository: brankovics/grabb (https://hub.docker.com/r/brankovics/grabb/).

Address of the bookmark: https://github.com/b-brankovics/grabb

]]> Rahul Nayak