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<channel>
	<title><![CDATA[BOL: Related items]]></title>
	<link>https://bioinformaticsonline.com/related/26356?offset=50</link>
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	<description><![CDATA[]]></description>
	
	<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/pages/view/42275/frequent-parameters-for-bioinformatics-tools</guid>
	<pubDate>Tue, 27 Oct 2020 19:42:32 -0500</pubDate>
	<link>https://bioinformaticsonline.com/pages/view/42275/frequent-parameters-for-bioinformatics-tools</link>
	<title><![CDATA[Frequent parameters for bioinformatics tools !]]></title>
	<description><![CDATA[<div><div>Third party executable parameters and options.</div><div>&nbsp;</div><div>Trimmomatic</div><div>&nbsp;</div><div>&ldquo;ILLUMINACLIP:...:2:30:10&rdquo;</div><div>&ldquo;LEADING:15&rdquo;</div><div>&ldquo;TRAILING:15&rdquo;</div><div>&ldquo;SLIDINGWINDOW:4:20&rdquo;</div><div>&ldquo;MINLEN:20&rdquo;</div><div>&ldquo;TOPHRED33&rdquo;</div><div>&nbsp;</div><div>Filtlong</div><div>--min_length 500</div><div>--min_mean_q 85</div><div>--min_window_q 65</div><div>&nbsp;</div><div>FastQ Screen</div><div>--aligner bowtie2' (bwa for PacBio)</div><div>--subset 1000 (for PacBio)</div><div>&nbsp;</div><div>SPAdes</div><div>--careful</div><div>--disable-gzip-output</div><div>--cov-cutoff auto</div><div>--phred-offset 33</div><div>&nbsp;</div><div>HGAP</div><div>Pbalign.task_options.min_accuracy: 70</div><div>Pbalign.task_options.no_split_subreads: false</div><div>Genomic_consensus.task_options.min_confidence: 40</div><div>falcon_ns.task_options.HGAP_GenomeLength_str:</div><div>6000000</div><div>Pbcoretools.task_options.read_length: 0</div><div>Genomic_consensus.task_options.use_score: 0</div><div>Pbalign.task_options.min_length: 50</div><div>Pbalign.task_options.algorithm_options: --minMatch 12</div><div>--bestn 10 --minPctSimilarity 70.0</div><div>Pbalign.task_options.hit_policy: randombest</div><div>Pbcoretools.task_options.other_filters: rq &gt;= 0.7</div><div>Pbalign.task_options.concordant: false</div><div>Genomic_consensus.task_options.min_coverage: 5</div><div>falcon_ns.task_options.HGAP_SeedCoverage_str: 30</div><div>falcon_ns.task_options.HGAP_AggressiveAsm_bool: false</div><div>Genomic_consensus.task_options.algorithm: best</div><div>falcon_ns.task_options.HGAP_SeedLengthCutoff_str: -1</div><div>Genomic_consensus.task_options.diploid: false</div><div>&nbsp;</div><div>MeDuSa</div><div>-random 100</div><div>&nbsp;</div><div>Prokka</div><div>--usegenus</div><div>--force</div><div>--addgenes</div><div>--rfam</div><div>--rawproduct</div><div>&nbsp;</div><div>cmsearch (taxonomy, 16S)</div><div>--rfam</div><div>--noali</div><div>&nbsp;</div><div>blastn (taxonomy, 16S)</div><div>-evalue 1E-10</div><div>&nbsp;</div><div>blastn (MLST)</div><div>-ungapped</div></div><div><div>-dust no</div><div>-evalue 1E-20</div><div>-word_size 32</div><div>-culling_limit 2</div><div>-perc_identity 95</div><div>&nbsp;</div><div>blastp (VF)</div><div>-culling_limit 2</div><div>&nbsp;</div><div>RGI (ABR)</div><div>--input_type contig</div><div>&nbsp;</div><div>bowtie2 (mapping)</div><div>--sensitive</div><div>&nbsp;</div><div>minimap2 (mapping)</div><div>-a</div><div>-x map-ont</div><div>&nbsp;</div><div>samtools mpileup (SNP&nbsp;detection)</div><div>-uRI</div><div>&nbsp;</div><div>bcftools call (SNP detection)</div><div>--variants-only</div><div>--skip-variants indels</div><div>--output-type v</div><div>--ploidy 1</div><div>-c</div><div>&nbsp;</div><div>SNPsift filter (SNP detection)</div><div>"( QUAL &gt;= 30 ) &amp; (( na FILTER ) | (FILTER = 'PASS')) &amp;</div><div>( DP &gt;= 20 ) &amp; ( MQ &gt;= 20 )"</div><div>&nbsp;</div><div>SNPeff ann (SNP detection)</div><div>-nodownload</div><div>-no-intron</div><div>-no-downstream</div><div>-no SPLICE_SITE_REGION</div><div>-upDownStreamLen 250</div><div>&nbsp;</div><div>bcftools consensus</div><div>(phylogenetic tree)</div><div>--haplotype 1</div><div>&nbsp;</div><div>fasttreemp</div><div>-nt</div><div>-boot 100</div><div>&nbsp;</div><div>roary</div><div>-e</div><div>-n</div><div>-cd 100</div><div>-g 100000</div></div>]]></description>
	<dc:creator>BioStar</dc:creator>
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<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/33014/synteny-portal-a-web-based-application-portal-for-synteny-block-analysis</guid>
	<pubDate>Wed, 24 May 2017 10:39:23 -0500</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/33014/synteny-portal-a-web-based-application-portal-for-synteny-block-analysis</link>
	<title><![CDATA[Synteny Portal: a web-based application portal for synteny block analysis]]></title>
	<description><![CDATA[<p><span>Synteny Portal, a versatile web-based application portal for constructing, visualizing and browsing synteny blocks. With Synteny Portal, users can easily (i) construct synteny blocks among multiple species by using prebuilt alignments in the UCSC genome browser database, (ii) visualize and download syntenic relationships as high-quality images, (iii) browse synteny blocks with genetic information and (iv) download the details of synteny blocks to be used as input for downstream synteny-based analyses, all in an intuitive and easy-to-use web-based interface. We believe that Synteny Portal will serve as a highly valuable tool that will enable biologists to easily perform comparative genomics studies by compensating limitations of existing tools. Synteny Portal is freely available at&nbsp;</span><a href="http://bioinfo.konkuk.ac.kr/synteny_portal" target="pmc_ext">http://bioinfo.konkuk.ac.kr/synteny_portal</a><span>.</span></p>
<p>http://bioinfo.konkuk.ac.kr/synteny_portal/</p><p>Address of the bookmark: <a href="http://bioinfo.konkuk.ac.kr/synteny_portal/" rel="nofollow">http://bioinfo.konkuk.ac.kr/synteny_portal/</a></p>]]></description>
	<dc:creator>Jit</dc:creator>
</item>
<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/17924/software-developed-in-pevsner-lab</guid>
	<pubDate>Mon, 06 Oct 2014 12:41:26 -0500</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/17924/software-developed-in-pevsner-lab</link>
	<title><![CDATA[Software developed in pevsner lab]]></title>
	<description><![CDATA[<div>
<div id="block-system-main">
<div>
<div id="node-7">
<div>
<div>
<div>
<div>
<p><a href="http://pevsnerlab.kennedykrieger.org/dragon.htm">DRAGON</a>: Database Referencing of Array Genes Online</p>
<p><a href="http://pevsnerlab.kennedykrieger.org/php/node/96">SNOMAD</a>: Standardization and Normalization of Microarray Data</p>
<p><a href="http://pevsnerlab.kennedykrieger.org/php/node/70">SNPduo</a>: SNP Analysis Between Two Individuals</p>
<p><a href="http://pevsnerlab.kennedykrieger.org/php/node/71">SNPtrio</a>: Analyzing and Visualizing and Inheritance Patterns in Trios</p>
<p><a href="http://pevsnerlab.kennedykrieger.org/php/node/64">SNPscan</a>: Data Analysis and Visualization of SNP Data</p>
<p><a href="http://pevsnerlab.kennedykrieger.org/php/node/64">pediSNP</a>: Analyze SNP Data From a Pedigree of Two Generations</p>
<p><a href="http://pevsnerlab.kennedykrieger.org/php/node/73">kcoeff</a>: Calculate Cotterman Coefficients of SNP Genotype Data</p>
<p><a href="http://pevsnerlab.kennedykrieger.org/php/node/113">triPOD:</a> Detects chromosomal abnormalities in parent-child trio-based microarray data</p>
</div>
</div>
</div>
</div>
</div>
</div>
</div>
</div><p>Address of the bookmark: <a href="http://pevsnerlab.kennedykrieger.org/php/?q=software" rel="nofollow">http://pevsnerlab.kennedykrieger.org/php/?q=software</a></p>]]></description>
	<dc:creator>Robert M Willioms</dc:creator>
</item>
<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/news/view/19090/deeptools</guid>
	<pubDate>Sat, 08 Nov 2014 15:02:08 -0600</pubDate>
	<link>https://bioinformaticsonline.com/news/view/19090/deeptools</link>
	<title><![CDATA[deepTools]]></title>
	<description><![CDATA[<p>deepTools addresses the challenge of handling the large amounts of data that are now routinely generated from DNA sequencing centers. To do so, deepTools contains useful modules to process the mapped reads data to create coverage files in standard bedGraph and bigWig file formats. By doing so, deepTools allows the creation of normalized coverage files or the comparison between two files (for example, treatment and control). Finally, using such normalized and standardized files, multiple visualizations can be created to identify enrichments with functional annotations of the genome.<br /><br />Publicaton: http://nar.oxfordjournals.org/content/early/2014/05/05/nar.gku365.full<br /><br />Source Code and Wiki: https://github.com/fidelram/deepTools/wiki<br /><br />Galaxy Tool Shed repository: http://toolshed.g2.bx.psu.edu/view/bgruening/deeptools<br /><br />and example Galaxy workflows: http://toolshed.g2.bx.psu.edu/view/bgruening/deeptools_workflows</p>]]></description>
	<dc:creator>Martin Jones</dc:creator>
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<item>
  <guid isPermaLink='true'>https://bioinformaticsonline.com/opportunity/view/24297/bioinformatics-walkin-at-nii</guid>
  <pubDate>Fri, 04 Sep 2015 21:48:15 -0500</pubDate>
  <link></link>
  <title><![CDATA[Bioinformatics WalkIn at NII]]></title>
  <description><![CDATA[
<p>ADVERTISEMENT OF WALK-IN-INTERVIEW</p>

<p>NAME OF THE POST : Bioinformatician (Part time 3 days in a week) (One Position only)</p>

<p>DURATION : One Year</p>

<p>NAME OF THE PROJECT : Next generation sequencing facility</p>

<p>EDUCATIONAL QUALIFICATIONS : At least a Masters degree in Bioinformatics and Bachelors degree in any stream of life sciences</p>

<p>REQUIREMENTS :</p>

<p>Around 5 years of experience and proven track record in next generation sequence data analysis (supported by publications in peer-reviewed journals), ability to analyze transcriptomics, Chip-seq, and small RNA –seq data.</p>

<p>: Should have the ability to analyze raw primary data generated by Illumina next generation sequencing platforms and create / troubleshoot custom analysis Pipelines.</p>

<p>Should have ability to handle all downstream secondary and tertiary data analysis using commercially available as well as open source softwares (transcriptomics, ChIP-seq, small RNA-seq)</p>

<p>Apart from these, the applicant should have knowledge of the following: Programming: Perl and Python. Operating system:</p>

<p>Linux and Windows. NGS Analysis tools: Maq, BWA, Bowtie, SAM tools, BEDTools, MACS, Galaxy, FastQC, Bismark, MEDIPS, Tophat, Cufflinks, AvadisNGS, CLC Genomics Workbench, Galaxy, BaseSpace, Trinity Statistics: Microsoft Excel and R. Database: MySQL Genome Browser: UCSC, Ensemble, IGV, IGB Motif Analysis Tools: MEME Suite, Transfac and RSAT Functional Annotation Tools: DAVID, GeneCodis, Gene Cards Networking Tools: Cytoscape</p>

<p>EMOLUMENTS : The incumbent will be paid a fee of Rs. 2000/- per sitting/ per day.</p>

<p>SCIENTIST NAME : Dr. Arnab Mukhopadhyay,</p>

<p>Staff Scientific V Next generation sequencing facility</p>

<p>SCIENTIST’S E-MAIL ID : arnab@nii.ac.in</p>

<p>WALK IN INTERVIEW ON : 18th September, 2015</p>

<p>REGISTRATION OF CANDIDATES: 10.30 AM to 11.00 AM</p>

<p>PLEASE NOTE- 1. CANDIDATE MAY FILL UP APPLICATION IN THE PRECRIBED FORMAT ALONG WITH NECESSARY DOCUMENTS FOR VERIFICATION. 2. APPLICATIONS CONTAINING INCOMPLETE INFORMATION SHALL NOT BE ENTERTAINED. 3. DATE OF PASSING THE EXAMINATIONS MUST BE INDICATED CLEARLY. 4. ONLY REGISTERED CANDIDATES WILL BE INTERVIEWED. 5. NO TA/DA WILL BE PAID FOR ATTENDING THE INTERVIEW PRESCRIBED FORM 1. NAME 2. FATHER’S NAME 3. MOTHER’S NAME 4. DATE OF BIRTH 5. SEX (MALE/FEMALE) 6. CATEGORY (SC/ ST/ OBC/ PH) 7. ADDRESS a. (CORRSPONDENCE) b. (PERMANENT) 8. E MAIL, TELEPHONE NO. &amp; MOBILE No (if any) 9. ACADEMIC &amp; PROFESSIONAL QUALIFICATIONS NAME OF EXAMINATION PASSED WITH SUBJECTS YEAR OF PASSING BOARD/ UNIVERSITY PERCENTAGE/ DIVISION REMARKS 10. PAST EXPERIENCE &amp; PRESENT EMPLOYMENT, IF ANY 11. CANDIDATES SHOULD STATE CLEARLY WHETHER THEY HAVE BEEN AWARDED PH.D DEGREE OR THESIS HAS BEEN SUBMITTED. 12. HAVE YOU APPLIED FOR A POSITION EARLIER IN THE INSTITUTE? IF SO:- (1) THE DETAILS OF THE PROJECT AND PROJECT INVESTIGATOR (2) IF CALLED FOR INVERVIEW, RESULTS THEREOF</p>

<p>More at http://www1.nii.res.in/sites/default/files/walkininterview-18sept2015.pdf</p>
]]></description>
</item>
<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/27463/bpipe-a-tool-for-running-and-managing-bioinformatics-pipelines</guid>
	<pubDate>Sat, 21 May 2016 22:42:16 -0500</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/27463/bpipe-a-tool-for-running-and-managing-bioinformatics-pipelines</link>
	<title><![CDATA[Bpipe - a tool for running and managing bioinformatics pipelines]]></title>
	<description><![CDATA[<p>Bpipe provides a platform for running big bioinformatics jobs that consist of a series of processing stages - known as 'pipelines'.</p>
<ul>
<li>January 20th, 2016 - New! Bpipe 0.9.9 released!</li>
<li>Download <a href="http://download.bpipe.org/versions/bpipe-0.9.9.tar.gz">latest</a>, <a href="http://download.bpipe.org">all</a></li>
<li><a href="http://docs.bpipe.org">Documentation</a></li>
<li><a href="https://groups.google.com/forum/#%21forum/bpipe-discuss">Mailing List</a> (Google Group)</li>
</ul>
<p>Bpipe has been published in <a href="http://bioinformatics.oxfordjournals.org/content/early/2012/04/11/bioinformatics.bts167.abstract">Bioinformatics</a>! If you use Bpipe, please cite:</p>
<p><em>Sadedin S, Pope B &amp; Oshlack A, Bpipe: A Tool for Running and Managing Bioinformatics Pipelines, Bioinformatics</em></p><p>Address of the bookmark: <a href="http://docs.bpipe.org/" rel="nofollow">http://docs.bpipe.org/</a></p>]]></description>
	<dc:creator>Radha Agarkar</dc:creator>
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	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/27959/darkhorse</guid>
	<pubDate>Wed, 22 Jun 2016 05:37:38 -0500</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/27959/darkhorse</link>
	<title><![CDATA[DarkHorse]]></title>
	<description><![CDATA[<p><em>DarkHorse</em>&nbsp;is a bioinformatic method for rapid, automated identification and ranking of phylogenetically atypical proteins on a genome-wide basis. It works by selecting potential ortholog matches from a reference database of amino acid sequences, then using these matches to calculate a lineage probability index (LPI) score for each genome protein.</p>
<p>LPI scores are inversely proportional to the phylogenetic distance between database match sequences and the query genome. These scores are useful not only for large-scale<em>de novo</em>&nbsp;predictions of horizontally transferred proteins, but can also serve as an independent quality control test for potential horizontal transfer candidates identified by alternative methods, especially those based on nucleic acid signatures. Candidates having high LPI scores are unlikely to have been horizontally transferred, since they are highly conserved among closely related organisms.</p>
<p>One unique and powerful feature of the DarkHorse HGT Candidate database is the opportunity to explore the phylogenetic background of potential HGT donors as well as recipients. The breadth of the database allows not only query sequences, but also their database match partners to be evaluated for sequence similarity or novelty compared to taxonomically related organisms.</p>
<p><em>DarkHorse</em>&nbsp;is configurable for varying degrees of phylogenetic granularity and protein sequence conservation. Users should consult the&nbsp;<a href="http://darkhorse.ucsd.edu/#references">references</a>&nbsp;cited below for a complete explanation of parameter selection and result interpretation. A brief&nbsp;<a href="http://darkhorse.ucsd.edu/tutorial.html">tutorial</a>&nbsp;page is also available on-line.</p><p>Address of the bookmark: <a href="http://darkhorse.ucsd.edu/download.html" rel="nofollow">http://darkhorse.ucsd.edu/download.html</a></p>]]></description>
	<dc:creator>Jit</dc:creator>
</item>
<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/28290/bioinformatics-tools-and-software</guid>
	<pubDate>Tue, 05 Jul 2016 10:02:26 -0500</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/28290/bioinformatics-tools-and-software</link>
	<title><![CDATA[Bioinformatics tools and software]]></title>
	<description><![CDATA[<p><a href="http://drive5.com/usearch">USEARCH &gt;</a><br><span>Extreme high-throughput sequence analysis. Orders of magnitude faster than BLAST.</span>&nbsp;<a href="http://drive5.com/muscle">MUSCLE &gt;</a><br><span>Multiple sequence alignment. Faster and more accurate than CLUSTALW.</span></p>
<p>&nbsp;<a href="http://drive5.com/uparse">UPARSE &gt;</a><br><span>OTU clustering for 16S and other marker genes. Highly accurate OTU sequences and improved diversity measures.</span>&nbsp;<a href="http://drive5.com/uchime">UCHIME &gt;</a><br><span>Chimeric sequence detection.</span>&nbsp;<a href="http://drive5.com/piler">PILER &gt;</a><br><span>De novo genome repeat finder.</span>&nbsp;<a href="http://drive5.com/pilercr">PILER-CR &gt;</a><br><span>Detection of CRISPR repeats in bacterial genomes.</span>&nbsp;<a href="http://drive5.com/qscore">QSCORE &gt;</a><br><span>Compare two multiple alignments for benchmarking.</span>&nbsp;<a href="http://drive5.com/pals">PALS &gt;</a><br><span>Whole-genome alignment.</span>&nbsp;<a href="http://drive5.com/muscle/prefab.htm">PREFAB &gt;</a><br><span>Protein Reference Alignment Database.</span>&nbsp;<a href="http://drive5.com/bench">MSA benchmark collection &gt;</a><br><span>Selected multiple alignment benchmarks in a standardized FASTA format.</span></p><p>Address of the bookmark: <a href="http://drive5.com/software.html" rel="nofollow">http://drive5.com/software.html</a></p>]]></description>
	<dc:creator>Jit</dc:creator>
</item>
<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/28915/useful-bioinformatics-tools</guid>
	<pubDate>Mon, 29 Aug 2016 04:08:12 -0500</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/28915/useful-bioinformatics-tools</link>
	<title><![CDATA[Useful Bioinformatics Tools]]></title>
	<description><![CDATA[<p>Collections of few handy tools for bioinformatician</p>
<p>http://molbiol-tools.ca/Convert.htm</p><p>Address of the bookmark: <a href="http://molbiol-tools.ca/Convert.htm" rel="nofollow">http://molbiol-tools.ca/Convert.htm</a></p>]]></description>
	<dc:creator>Poonam Mahapatra</dc:creator>
</item>
<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/29276/murasaki</guid>
	<pubDate>Fri, 30 Sep 2016 10:22:30 -0500</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/29276/murasaki</link>
	<title><![CDATA[Murasaki]]></title>
	<description><![CDATA[<p>Murasaki is an anchor alignment program that is</p>
<ul style="margin-left: 16px;">
<li>exteremely fast (17 CPU hours for whole Human x Mouse genome (with 40 nodes: 35 wall minutes), or 8 mammals in 21 CPU hours (42 wall minutes))</li>
<li>scalable (Arbitrarily parallelizable across multiple nodes using MPI)</li>
<li>memory efficient. (Even a single node with 16GB of ram can handle over 1Gbp of sequence)</li>
<li>unlimited by pattern length or selection</li>
<li>repeat tolerant</li>
</ul>
<p><img src="http://murasaki.dna.bio.keio.ac.jp/9mammals-small.png" width="500" height="375" alt="image" style="border: 0px;"></p><p>Address of the bookmark: <a href="http://murasaki.dna.bio.keio.ac.jp/wiki/index.php?Murasaki" rel="nofollow">http://murasaki.dna.bio.keio.ac.jp/wiki/index.php?Murasaki</a></p>]]></description>
	<dc:creator>Anjana</dc:creator>
</item>

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