BOL: Related items

Steps to find palindrome in genomes !

BioStar — Thu, 09 Mar 2023 02:56:54 -0600

Palindromes are sequences of nucleotides that read the same backward as forward. They can be present in genomes and have various biological functions. Here are some methods for discovering palindromes in genomes:

Direct sequence search: One of the simplest ways to discover palindromes is to search the genome sequence directly for palindromic sequences using pattern matching tools, such as regular expressions or string algorithms. This approach can be useful for discovering simple palindromes, but may miss more complex palindromic structures.
Dot plot analysis: Dot plot analysis is a graphical method that can be used to identify palindromic regions in a genome. It involves plotting the genome sequence against itself and examining the diagonal patterns that emerge. Palindromic regions will appear as symmetrical patterns along the diagonal.
Restriction enzyme analysis: Some restriction enzymes, such as EcoRI and HindIII, recognize palindromic sequences and cleave DNA at these sites. By digesting the genome with these enzymes and examining the resulting fragments, palindromic regions can be identified.
Next-generation sequencing: High-throughput sequencing technologies, such as PacBio and Oxford Nanopore, can generate long reads that can span entire palindromic regions. By mapping these reads to the genome, palindromic regions can be identified and characterized.
Comparative genomics: Comparing the genomes of related species can also reveal palindromic regions that are conserved across evolutionarily divergent lineages. This approach can help identify functional palindromes that are under selective pressure.

Overall, the discovery of palindromic sequences in genomes can be accomplished using a variety of methods, each with their own advantages and limitations. A combination of these methods can provide a comprehensive understanding of the palindromic landscape of a genome.

QuorUM: An Error Corrector for Illumina Reads

Jit — Wed, 08 Nov 2017 11:40:41 -0600

Illumina Sequencing data can provide high coverage of a genome by relatively short (most often 100 bp to 150 bp) reads at a low cost. Even with low (advertised 1%) error rate, 100 × coverage Illumina data on average has an error in some read at every base in the genome. These errors make handling the data more complicated because they result in a large number of low-count erroneous k-mers in the reads. However, there is enough information in the reads to correct most of the sequencing errors, thus making subsequent use of the data (e.g. for mapping or assembly) easier. Here we use the term “error correction” to denote the reduction in errors due to both changes in individual bases and trimming of unusable sequence. We developed an error correction software called QuorUM. QuorUM is mainly aimed at error correcting Illumina reads for subsequent assembly. It is designed around the novel idea of minimizing the number of distinct erroneous k-mers in the output reads and preserving the most true k-mers, and we introduce a composite statistic π that measures how successful we are at achieving this dual goal. We evaluate the performance of QuorUM by correcting actual Illumina reads from genomes for which a reference assembly is available.

QuorUM is distributed as an independent software package and as a module of the MaSuRCA assembly software. Both are available under the GPL open source license at http://www.genome.umd.edu.

Address of the bookmark: http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0130821

NxRepair: error correction in de novo assemblies using Nextera Mate Pair Reads

BioStar — Thu, 24 Jan 2019 10:35:12 -0600

NxRepair is a python module that automatically detects large structural errors in de novo assemblies using Nextera mate pair reads. The decector will break a contig at the site of an identified misassembly and will generate a new fasta file containing both the corrected contigs and the correct, unaffected contigs.

https://nxrepair.readthedocs.io/en/latest/tutorial.html

nxrepair aligned_matepairs.bam assemblyfasta.fasta error_locations.csv new_fasta.fasta

Address of the bookmark: https://github.com/rebeccaroisin/nxrepair

BEAR: Better Emulation for Artificial Reads

BioStar — Sat, 06 Jul 2024 04:27:53 -0500

Created by Stephen Johnson, Brett Trost, Dr. Jeffrey R. Long, Dr. Anthony Kusalik University of Saskatchewan, Department of Computer Science

BEAR is intended to be an easy-to-use collection of scripts for generating simulated WGS metagenomic reads with read lengths, quality scores, error profiles, and species abundances derived from real user-supplied WGS data.

Address of the bookmark: https://github.com/sej917/BEAR

AGRICUL AGRICULTURAL SCIENTISTS RECRUITMENT BOARD TURAL SCIENTISTS RECRUITMENT BOARD NEW DELHI-110 012

Wed, 11 Mar 2015 09:18:37 -0500

ADVERTISEMENT NO. 01/2015

PRINCIPAL SCIENTIST Pay Band: Minimum pay of `43,000 in the PB-4 of `37400-67000/- + RGP of `10,000/-.

Age: The candidates must not have attained the age of 52 years as on 24.03.2015. There shall be no age limit for the Council’s employees.

ICAR-NATIONAL INSTITUTE OF BIOTIC STRESS MANAGEMENT, RAIPUR (CHHATTISGARH)

57. Principal Scientist (Agricultural Entomology) (Two post)

Qualifications Essential:

(i) Doctoral degree in Agricultural Entomology including relevant basic sciences.

(ii) 10 years experience in the relevant subject out of which at least 8 years should be as Scientist/ Lecturer/Extension Specialist or in an equivalent position in the Pay Band- 3 of `15600-39100 with Grade Pay of `5400/`6000/`7000/`8000 and 2 years as a Senior Scientist or in an equivalent position in the Pay Band- 4 of ` 37400-67000 with Grade Pay of ` 8700/ ` 9000.

(iii) The candidate should have made contribution to research/teaching/extension education as evidenced by published work/innovations and impact.

Desirable:

(i) Experience of using frontiers research tools in management of insect pests of crop plants.

(ii) Evidence of contributions to relevant field through publications/ patents/citation index to suggest a vision/perspective in biotic stress research.

61. Principal Scientist (Bioinformatics) (One post)

Qualifications Essential:

(i) Doctoral degree in Bioinformatics including relevant basic sciences. (ii) & (iii) As in item no. 57 above.

Desirable:

(i) Experience of using bioinformatics for advancement of knowledge and for research on biotic stress management.

(ii) Evidence of contributions to relevant field through publications/patents/citation index to suggest a vision/perspective in biotic stress research.

http://asrb.org.in/administrator/uploads_dir/1424859407english.pdf

Research Associate at National Research Centre on Plant Biotechnology New Delhi

Mon, 16 Mar 2015 03:22:26 -0500

Walk-in interview will be held on 24-03-2015 at 10:00 AM at NRCPB, New Delhi for filling Research Associate and Senior Research Fellow positions as mentioned below. The positions are temporary and are initially offered for a period of one year. Details such as emoluments, qualifications, application format etc., are given below. Desirous candidates should report for interview latest by 10:30 AM with the application in the prescribed format, copies and originals of certificates, thesis and documents. No TA/DA will be provided for attending the interview.

ICAR-NPTC: Fibre development in flax/linseed.

(Job # 1) Research Associate (one) (Bioinformatics)

Rs.24000+ 30% HRA) for Ph.D. and for M. Sc Rs.23000/‐ (+ 30% HRA)

Ph.D. Degree in Bioinformatics/Molecular Biology/Biotechnology/ Genetics/allied sciences; or M. Sc in Bioinformatics/ Biotechnology/Life Sciences/ allied sciences with 1st division or 60% marks or equivalent overall grade point average with at least two years of research experience as evidenced from Fellowship/ Associate ship. 2 years research experience in bioinformatic data analysis/molecular biology techniques, and high throughput DNA/RNA sequencing, and transcriptome data analysis. Research paper with IF>1 will be desirable

ICAR-NPTC: Shade avoidance/low-light tolerance in rice.

General Terms & Conditions applicable to all the positions:
Age Limit: 35 years max. (5 years relaxation for SC/ST/OBC and woman candidates as per ICAR rules).
The positions are purely temporary, on a contractual basis and are initially offered for one year.
Originals must be shown for verification. 7. Research experience (Experience certificate from previous employer to be attached): I hereby declare that the information provided above is true to the best of my knowledge. Date: Signature

www.nrcpb.org/sites/default/files/ICAR-NPTC%20DBT%20RA%20SRF%20interview%2024th%20March.pdf

Narcis Fernandez-Fuentes Lab

Mon, 25 May 2015 07:30:00 -0500

Welcome to our web-site compiling all the research-related activities of the group. Our research interests relate to a number of areas within Bioinformatics. We have a long-standing interest in protein structure prediction and structure-to-function relationships. We work in the study of biomolecular interactions, modeling of protein complexes, the study and characterization of protein-protein interactions, peptide design, modeling of genetic variation, structure-based protein design and different aspects of Plant Bioinformatics. Take a look at the our databases and servers and the list of publications for more information.

More at http://www.bioinsilico.org/

Postdoctoral Fellowship in Bioinformatics at pesolelab

Thu, 01 Oct 2015 07:20:48 -0500

Job Description: Bioinformatics postdoc positions are available in the area of genomics with main focus on exome and RNAseq technologies by ultra high-throughput sequencing platforms. Successful applicants should have the following qualities:

1) demonstrated experience in Bioinformatics research,
2) programing experience (python and/or R, C and C++ are very welcome),
3) knowledge of Linux/Unix environment,
4) experience in handling deep-seq data,
5) highly motivated and hard working, and
6) interested to work with a multi-disciplinary team combining bioinformatics, genomics, computational biology approaches with experimental biology.

Our research interest covers different areas of bioinformatics and genomics in order to achieve a deeper understanding of gene and genome structure and function (please look at our PubMed publications for more details about our research http://www.ncbi.nlm.nih.gov/pubmed/?term=pesole+g).

Interested applicants should email the curriculum vitae to Prof. Graziano Pesole at graziano.pesole@uniba.it or Dr. Ernesto Picardi at Ernesto.picardi@uniba.it.

Start date: immediate

Duration: up to 24 months
Contact Person (Referent): Ernesto Picardi
Ref. E-Mail: ernesto.picardi@uniba.it
Tel: +390805443308
Fax: +390805443317

Group Web Page: http://www.pesolelab.it/

Project Fellow Positions at CSIR-IHBT Palampur

Tue, 01 Dec 2015 05:45:58 -0600

Walk-in-Interview is scheduled to be held on the date as mentioned below for selection of Suitable candidates in the following areas under the different Sponsored/CSIR Networked Projects on purely temporary basis for the duration of the project(s) or till completion of projects whichever is earlier:

Sponsored/CSIR Networked Project:
(i) Genomics and Informatics Solutions for Integrating Biology (GENESIS)" [BSC-0121] (up to March, 2017).
(ii) Profiling and characterization of early phase differential mi-RNA (s) responsible for downstream developmen of insulin resistance in hMSC derived adipocytes. (GAP-0188) [up to 31.03.2018].

Position: Project Fellow (2 position)
Age : 28 years as on 18.12.15
Salary : Rs.12,000/- P.M.
Rs.14,000/- P.M.
as per the funds provisions in the respective projects.
Eligibility Criteria : 1st Class B. Tech. in Bioinformatics/ Computational Biology
OR
M.Sc. in Bioinformatics/ Computational Biology with 55% marks
OR
M.Tech. in Bioinformatics/ Computational Biology with 55% marks
OR
M.Sc. in any field of Life Science with Diploma in Bioinformatics

Selection Procedure : Walk In Interview

Date : 18 Dec , 2015
Time : 10:00 A.M.
Venue : CSIR-IHBT Palampur (H.P.)

JRF Bioinformatics at National Chemical Laboratory

Sun, 03 Jan 2016 05:59:05 -0600

Junior Project Fellow Bioinformatics
Eligibility : ME/M.Tech, MSc(Bio-Informatics)
Location : Pune
Last Date : 08 Jan 2016
Hiring Process : Written-test, Face to Face Interview
National Chemical Laboratory

Junior Project Fellow Jobs opportunity in National Chemical Laboratory on contract basis
Project Code : BSC0117
Title of the Project : Plant?Microbe and Soil Interactions (PMSI)
No. of Post : 01
Qualification : M.Tech. / M.Sc. in Bioinformatics from a recognized university with minimum of 55% marks (aggregate) and sound Bioinformatics knowledge / experience
Desirable : Good knowledge of Linux (command line and GUI) and SQL; Java / Perl / Python / R / Bash programing / scripting; Analysis of NGS data; Protein modeling / docking; Development and maintenance of web & database servers, etc
Emoluments : Rs. 16,000/?
Age Limit : 28 Years
Selection Procedure : Written Test / Interview
How to apply
Applications neatly typed in the prescribed proforma (enclosed herewith) duly completed and signed together with photo?copies of relevant certificates/ testimonials and photograph should be addressed to : The Head, Biochemical Sciences Division, Attn : Dr. Narendra Kadoo, CSIR?National Chemical Laboratory, Dr. Homi Bhabha Road, Pashan, Pune 411 008, so as to reach on or before 08/01/2016. Please superscribe the envelop “Application for Junior Project Fellow (Project: BSC0117)”.

More at http://www.ncl-india.org/files/JoinUs/JobVacancies/TemporaryJobs.aspx?