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	<title><![CDATA[BOL: Related items]]></title>
	<link>https://bioinformaticsonline.com/related/26573?offset=510</link>
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	<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/file/view/22053/binc-sample-question-paper</guid>
	<pubDate>Thu, 16 Apr 2015 09:16:18 -0500</pubDate>
	<link>https://bioinformaticsonline.com/file/view/22053/binc-sample-question-paper</link>
	<title><![CDATA[BINC Sample Question Paper !!!]]></title>
	<description><![CDATA[<p>BINC sample question paper. Wish you all the best for BINC examination.</p>]]></description>
	<dc:creator>Jitendra Narayan</dc:creator>
	<enclosure url="https://bioinformaticsonline.com/file/download/22053" length="4041" type="text/plain" />
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<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/44223/ale-assembly-likelihood-estimator</guid>
	<pubDate>Wed, 08 Mar 2023 01:39:33 -0600</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/44223/ale-assembly-likelihood-estimator</link>
	<title><![CDATA[ALE: Assembly Likelihood Estimator]]></title>
	<description><![CDATA[<p>Just import the assembly, bam and ALE scores. You can convert the .ale file to a set of .wig files with ale2wiggle.py and IGV can read those directly.&nbsp; Depending on your genome size you may want to convert the .wig files to the BigWig format.</p><p>Address of the bookmark: <a href="https://github.com/sc932/ALE" rel="nofollow">https://github.com/sc932/ALE</a></p>]]></description>
	<dc:creator>BioStar</dc:creator>
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	<guid isPermaLink="true">https://bioinformaticsonline.com/pages/view/44371/steps-to-find-all-the-repeats-in-the-genome</guid>
	<pubDate>Thu, 31 Aug 2023 02:43:28 -0500</pubDate>
	<link>https://bioinformaticsonline.com/pages/view/44371/steps-to-find-all-the-repeats-in-the-genome</link>
	<title><![CDATA[Steps to find all the repeats in the genome !]]></title>
	<description><![CDATA[<div><p>To find repeats in a genome from 2 to 9 length using a Perl script, you can use the RepeatMasker tool with the "--length" option<a href="https://mobilednajournal.biomedcentral.com/articles/10.1186/1759-8753-5-13" target="_blank">[0]</a>. Here's a step-by-step guide:</p></div><div><ol>
<li>Install RepeatMasker: First, you need to install RepeatMasker on your system. You can download it from the RepeatMasker website<a href="https://mobilednajournal.biomedcentral.com/articles/10.1186/1759-8753-5-13" target="_blank">[0]</a>.</li>
</ol></div><div><ol>
<li>Prepare the genome sequence: Make sure you have the genome sequence in a FASTA file format. Let's assume the file is named "genome.fasta".</li>
</ol><blockquote><p>./RepeatMasker -pa &lt;number_of_processors&gt; -nolow -norna -no_is -div &lt;divergence_value&gt; -lib RepeatMaskerLib.embl -gff -xsmall -small -poly -species &lt;species_name&gt; -dir &lt;output_directory&gt; -length &lt;min_length&gt;-&lt;max_length&gt; genome.fasta</p></blockquote><div><p>Replace the following placeholders with appropriate values:</p><ul>
<li><code>&lt;number_of_processors&gt;</code>: The number of processors/threads you want to use for parallel processing.</li>
<li><code>&lt;divergence_value&gt;</code>: The divergence value for the species you are analyzing. You can find divergence values for different species in the RepeatMasker documentation<a href="https://mobilednajournal.biomedcentral.com/articles/10.1186/1759-8753-5-13" target="_blank">[0]</a>.</li>
<li><code>&lt;species_name&gt;</code>: The name of the species you are analyzing.</li>
<li><code>&lt;output_directory&gt;</code>: The directory where you want the output files to be saved.</li>
<li><code>&lt;min_length&gt;</code>&nbsp;and&nbsp;<code>&lt;max_length&gt;</code>: The minimum and maximum lengths of the repeats you want to find (in this case, 2 and 9).</li>
</ul></div><div><ol>
<li>Analyze the output: RepeatMasker will generate several output files, including a .out file. You can parse this file to extract the information you need. There is a Perl tool called "one_code_to_find_them_all.pl" that can help you parse RepeatMasker output files<a href="https://mobilednajournal.biomedcentral.com/articles/10.1186/1759-8753-5-13" target="_blank">[0]</a>. You can download it from the source provided.</li>
</ol></div><div><ol>
<li>Use the provided Perl script: Once you have the "one_code_to_find_them_all.pl" script, you can run it to conveniently parse the RepeatMasker output files. Here's an example of how to use it:</li>
</ol><blockquote><p>perl one_code_to_find_them_all.pl --rm &lt;RepeatMasker_out_file&gt; --length &lt;length_file&gt;</p></blockquote></div><p>&nbsp;</p></div><div><div><p>Replace&nbsp;<code>&lt;RepeatMasker_out_file&gt;</code>&nbsp;with the path to your RepeatMasker .out file, and&nbsp;<code>&lt;length_file&gt;</code>&nbsp;with the path to a file containing the lengths of the reference elements.</p></div><div><p>This script will generate several output files, including .log.txt and .copynumber.csv, which contain quantitative information about the identified repeat elements.</p></div><div><p>Remember to adjust the parameters and options according to your specific needs and the characteristics of your genome.</p></div></div>]]></description>
	<dc:creator>Neel</dc:creator>
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  <guid isPermaLink='true'>https://bioinformaticsonline.com/opportunity/view/22239/jrf-bioinformatics-at-national-institute-of-high-security-animal-diseases-icar</guid>
  <pubDate>Tue, 28 Apr 2015 02:21:15 -0500</pubDate>
  <link></link>
  <title><![CDATA[JRF Bioinformatics at National Institute of High Security Animal Diseases (ICAR)]]></title>
  <description><![CDATA[
<p>F.No. 4-59/2013-NIHSAD Dated: 21st April, 2015</p>

<p>SRF/ JRF job vacancies in National Institute of High Security Animal Diseases (ICAR)</p>

<p>Name of Post : JRF</p>

<p>No. of Post : 01</p>

<p>Qualification : M.V.Sc or M.Tech./M.Sc. (preferably with NET qualification) in one of following disciplines. (Biotechnology/Molecular Biology/Genetics/Microbiology/Bioinformatics or equivalent Life Sciences discipline). Desirable: Working Knowledge in the areas of Recombinant DNA Techniques, Cell Culture, Handling Laboratory Animals, Genomics.</p>

<p>Emolument : Rs.16,000/-</p>

<p>Age Limit : Up to 30 years</p>

<p>Name of Post : Project Assistant</p>

<p>No of Post : 01</p>

<p>Qualifications : First class M.Sc. /B.E/B.Tech. in one of the following disciplines Biotechnology/ Bioinformatics/ Microbiology or equivalent Life Sciences discipline). Desirable : Exposure of working in research environment, Good command over written/spoken English and computer applications.</p>

<p>Emolument : Rs.8000/-</p>

<p>Age Limit : Upto 28 years</p>

<p>Name of Post : Project Assistant</p>

<p>No of Post : 01</p>

<p>Qualification : First class M.Sc. /B.V.Sc. and A.H., B.Tech. /B.E. in Life Sciences and related areas. Desirable: Exposure of working in research environment, Good command over written/type written/spoken English and computer applications. </p>

<p>Emoluments : Rs. 8000/-</p>

<p>Age Limit : Up to 28 years<br />How to apply</p>

<p>Desirous candidates may send their applications by e-mail (techcell@hsadl.nic.in ) followed by post in the prescribed proforma latest by 11/05/2015. Walk-in-Interview will be held at NIHSAD, Kokta Road, Anand Nagar, Bhopal-462022.</p>

<p>http://www.nihsad.nic.in/pdf/Advt.pdf</p>
]]></description>
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	<guid isPermaLink="true">https://bioinformaticsonline.com/pages/view/44637/tools-to-access-the-quality-of-your-assembled-genome</guid>
	<pubDate>Thu, 08 Aug 2024 23:31:18 -0500</pubDate>
	<link>https://bioinformaticsonline.com/pages/view/44637/tools-to-access-the-quality-of-your-assembled-genome</link>
	<title><![CDATA[Tools to access the quality of your assembled genome !]]></title>
	<description><![CDATA[<ul dir="auto">
<li><a href="https://github.com/linsalrob/fasta_validator">FASTA VALIDATOR</a>&nbsp;+&nbsp;<a href="https://github.com/shenwei356/seqkit">SEQKIT RMDUP</a>: FASTA validation</li>
<li><a href="https://genometools.org/tools/gt_gff3validator.html">GENOMETOOLS GT GFF3VALIDATOR</a>: GFF3 validation</li>
<li><a href="https://github.com/PlantandFoodResearch/assemblathon2-analysis/blob/a93cba25d847434f7eadc04e63b58c567c46a56d/assemblathon_stats.pl">ASSEMBLATHON STATS</a>: Assembly statistics</li>
<li><a href="https://genometools.org/tools/gt_stat.html">GENOMETOOLS GT STAT</a>: Annotation statistics</li>
<li><a href="https://github.com/ncbi/fcs">NCBI FCS ADAPTOR</a>: Adaptor contamination pass/fail</li>
<li><a href="https://github.com/ncbi/fcs">NCBI FCS GX</a>: Foreign organism contamination pass/fail</li>
<li><a href="https://gitlab.com/ezlab/busco">BUSCO</a>: Gene-space completeness estimation</li>
<li><a href="https://github.com/tolkit/telomeric-identifier">TIDK</a>: Telomere repeat identification</li>
<li><a href="https://github.com/oushujun/LTR_retriever/blob/master/LAI">LAI</a>: Continuity of repetitive sequences</li>
<li><a href="https://github.com/DerrickWood/kraken2">KRAKEN2</a>: Taxonomy classification</li>
<li><a href="https://github.com/igvteam/juicebox.js">HIC CONTACT MAP</a>: Alignment and visualisation of HiC data</li>
<li><a href="https://github.com/mummer4/mummer">MUMMER</a>&nbsp;&rarr;&nbsp;<a href="http://circos.ca/documentation/">CIRCOS</a>&nbsp;+&nbsp;<a href="https://plotly.com/">DOTPLOT</a>&nbsp;&amp;&nbsp;<a href="https://github.com/lh3/minimap2">MINIMAP2</a>&nbsp;&rarr;&nbsp;<a href="https://github.com/schneebergerlab/plotsr">PLOTSR</a>: Synteny analysis</li>
<li><a href="https://github.com/marbl/merqury">MERQURY</a>: K-mer completeness, consensus quality and phasing assessment</li>
</ul>]]></description>
	<dc:creator>LEGE</dc:creator>
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  <guid isPermaLink='true'>https://bioinformaticsonline.com/opportunity/view/22285/project-associate-bioinformatics-iit-mandi</guid>
  <pubDate>Wed, 06 May 2015 06:18:47 -0500</pubDate>
  <link></link>
  <title><![CDATA[Project Associate Bioinformatics @ IIT Mandi]]></title>
  <description><![CDATA[
<p>Eligibility : MSc(Bio-Informatics, Bio-Tech), BSc, BE/B.Tech(Bio-Medical /Bio-Technology Engg, CSE)</p>

<p>Location : Kulu</p>

<p>Job Category : Govt Jobs, Research</p>

<p>Last Date : 20 May 2015</p>

<p>Job Type : Full Time</p>

<p>Hiring Process : Walk - In<br />IIT Mandi - Job Details<br />IIT Mandi</p>

<p>Project Associate Bioinformatics Job vacancies in IIT Mandi on purely temporary</p>

<p>Project: “Exploring the Human Microbiome: A hunt for the candidates for Pre- and Pro-biotics.”</p>

<p>Minimum Qualification and Experience: M.Sc. in Bioinformatics OR B.Tech / BE in Bioinformatics OR M. Sc. in Biotechnology / Life sciences or related areas with Diploma or relevant experience in Bioinformatics OR B.Tech in Biotechnology / Computer Science or MCA or B. Sc. in Life Sciences or related areas with PG diploma in Bioinformatics. Candidates with experience in NGS data handling and analysis will be preferred. Tenure: Initially for one year, extendable based upon performance.</p>

<p>No of Posts: 01</p>

<p>Salary: Rs. 12000- 18000/- per month.</p>

<p>How to apply</p>

<p>Interested candidates can come for a Walk-in-Interview on 20th May 2015 starting at 8:30 AM at the Academic Block, Indian Institute of Technology (IIT), Mandi, Vallabh College Campus, Near Bus Stand, Mandi, HP. The candidates should bring along their curriculum vitae (CV) and copies of educational and experience certificates. In case of any queries please contact: Dr. Tulika Prakash Srivastava (Principal Investigator), Indian Institute of Technology Mandi, Near Bus Stand Mandi - 175 001, Himachal Pradesh.</p>

<p>More at</p>

<p>http://www.iitmandi.ac.in/administration/advrt/Walk-in-interview_Ad.pdf</p>
]]></description>
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	<guid isPermaLink="true">https://bioinformaticsonline.com/blog/view/44766/genome-simulation-with-slim-and-msprime</guid>
	<pubDate>Fri, 31 Jan 2025 12:47:43 -0600</pubDate>
	<link>https://bioinformaticsonline.com/blog/view/44766/genome-simulation-with-slim-and-msprime</link>
	<title><![CDATA[Genome Simulation with SLiM and msprime]]></title>
	<description><![CDATA[<p>Genome simulation is an essential tool in population genetics, enabling researchers to model evolutionary processes and study genetic variation. Two widely used simulation tools in this field are <strong style="font-size: 12.8px;">SLiM</strong><span style="font-size: 12.8px; font-weight: normal;"> and </span><strong style="font-size: 12.8px;">msprime</strong><span style="font-size: 12.8px; font-weight: normal;">. While both serve different purposes, they can be used together with the </span><strong style="font-size: 12.8px;">slendr</strong><span style="font-size: 12.8px; font-weight: normal;"> framework to compare simulation outputs effectively.</span></p><h2>Overview of SLiM and msprime</h2><h3>SLiM: Forward Genetic Simulator</h3><p>SLiM is a <strong>free, open-source</strong> tool designed for forward genetic simulations. It allows researchers to model complex evolutionary scenarios, including selection, recombination, and demographic events, making it particularly useful for studying adaptation and selection in populations.</p><p><strong>Key Features of SLiM:</strong></p><ul>
<li>
<p>Simulates population evolution forward in time</p>
</li>
<li>
<p>Supports custom evolutionary models using an embedded scripting language</p>
</li>
<li>
<p>Allows modeling of spatial and ecological dynamics</p>
</li>
<li>
<p>Provides high flexibility and extensibility for user-defined scenarios</p>
</li>
<li>
<p>Available on GitHub as an open-source project</p>
</li>
</ul><h3>msprime: Ancestry and Mutation Simulator</h3><p>msprime is an efficient, <strong>open-source</strong> tool that simulates ancestry and mutations using a coalescent framework. It is known for its high-speed performance and low memory requirements, making it a popular choice for large-scale genomic simulations.</p><p><strong>Key Features of msprime:</strong></p><ul>
<li>
<p>Implements coalescent simulations for ancestry modeling</p>
</li>
<li>
<p>Efficiently simulates large population histories</p>
</li>
<li>
<p>Supports the addition of mutations to genealogies</p>
</li>
<li>
<p>Developed using an open-source community model</p>
</li>
<li>
<p>Often faster and more memory-efficient than alternative simulators</p>
</li>
</ul><h2>Using SLiM and msprime with slendr</h2><p>Both SLiM and msprime can be integrated with <strong>slendr</strong>, a framework that facilitates structured population genetic simulations. This integration allows for seamless comparison of simulation outputs.</p><h3>How They Work Together:</h3><ul>
<li>
<p>SLiM and msprime simulations can be analyzed within slendr.</p>
</li>
<li>
<p>The <strong>ts_read()</strong> function in slendr enables loading and comparing tree sequence outputs from both simulators.</p>
</li>
<li>
<p>This integration allows researchers to validate simulation results and gain deeper insights into evolutionary processes.</p>
</li>
</ul><h2>Performance Considerations</h2><p>While SLiM offers powerful forward simulations with extensive customization, msprime is often preferred for its <strong>speed and memory efficiency</strong> when simulating ancestry and mutations. The choice between the two depends on the research goals:</p><ul>
<li>
<p><strong>For detailed evolutionary modeling with selection and recombination:</strong> Use SLiM.</p>
</li>
<li>
<p><strong>For large-scale coalescent simulations with mutations:</strong> Use msprime.</p>
</li>
<li>
<p><strong>For comparing different simulation models and their outputs:</strong> Use slendr to integrate SLiM and msprime results.</p>
</li>
</ul><h2>Conclusion</h2><p>SLiM and msprime are valuable tools for genome simulation, each serving distinct but complementary purposes in population genetics research. By leveraging the strengths of both simulators with slendr, researchers can conduct robust and efficient evolutionary simulations, enhancing our understanding of genetic diversity and adaptation.</p><p>For more information, check out the official GitHub repositories for <strong>SLiM</strong> and <strong>msprime</strong>, and explore the <strong>slendr</strong> framework for streamlined simulation workflow</p>]]></description>
	<dc:creator>BioStar</dc:creator>
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  <guid isPermaLink='true'>https://bioinformaticsonline.com/opportunity/view/22317/project-associate-bioinformatics-central-food-technological-research-institute-cftri</guid>
  <pubDate>Tue, 19 May 2015 07:01:12 -0500</pubDate>
  <link></link>
  <title><![CDATA[Project Associate Bioinformatics @ Central Food Technological Research Institute (CFTRI)]]></title>
  <description><![CDATA[
<p>Central Food Technological Research Institute (CFTRI)</p>

<p>Project Assistant (Level-II) job position in Central Food Technological Research Institute (CFTRI) on a temporary contractual basis in the research project (GAP 0469) funded by Science &amp; Engineering Research Board (SERB), Government of India, New Delhi tenable at the Lipidomics Centre, CSIR-CFTRI, Mysore, Karnataka</p>

<p>Name of the Position : </p>

<p>Qualification : First class M. Sc. in Biochemistry/Microbiology/Genetics/ Bioinformatics with good academic record and preferably with experience in molecular biology techniques and basic knowledge of molecular biology and biological chemistry</p>

<p>Emoluments : Rs. 12,000/- per month (Consolidated)</p>

<p>Age Limit : The upper age limit for applying shall be 28 years (as on 22-5-2015), which is relaxed for candidates belonging to Scheduled Castes/Schedule Tribes, Women, Persons with Disabilities (PWD) and OBCs as per GoI norms. <br /> <br />How to apply</p>

<p>Eligible candidates may send their complete Bio-data with e-mail address/contact phone number along with attested copies of the necessary certificates through post to Prof. Ram Rajasekharan, Lipidomics Centre, Department of Lipid Science, CSIR-CFTRI, Mysore-570 020, Karnataka (email: ram@cftri.res.in) on or before 22.05.2015</p>

<p>More at http://www.cftri.com/pa_gap0469.html</p>
]]></description>
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	<guid isPermaLink="true">https://bioinformaticsonline.com/view/119</guid>
	<pubDate>Wed, 10 Jul 2013 14:35:34 -0500</pubDate>
	<link>https://bioinformaticsonline.com/view/119</link>
	<title><![CDATA[Which are the best statistical programming languages to study for a bioinformatician?]]></title>
	<description><![CDATA[<p><span>In Bio-informatics based&nbsp;genome sequencing and predicting metabolic pathways&nbsp;research jobs&nbsp;I used Matlab, SAS, SPSS, R and several Bioconductor packages. Matlab had a lot of powerful tools and was easy to use, whereas SPSS is for non-programmers and R need programming skills. I am wondering what other people think is best? or there might not be one specific language but a few that lend themselves best to Bio-informatics work that is math heavy and deals with a large amount of data.</span></p>]]></description>
	<dc:creator>Jitendra Narayan</dc:creator>
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  <guid isPermaLink='true'>https://bioinformaticsonline.com/opportunity/view/22430/nrco-vacancies-for-junior-research-fellow-%E2%80%93-pakyong-sikkim</guid>
  <pubDate>Thu, 28 May 2015 19:10:07 -0500</pubDate>
  <link></link>
  <title><![CDATA[NRCO Vacancies For Junior Research Fellow – Pakyong, Sikkim]]></title>
  <description><![CDATA[
<p>Junior Research Fellow<br />Pay Scale:Rs 25,000/-<br />Educational Requirements:MSc (with NET qualification) / M.Tech degree (with or without NET) with minimum 55% marks in Biotechnology/ Bioinformatics/ Molecular Biology or any other related field.<br />Other Qualification:Computer Skills (Linux, Perl, Java, MySQL) with experience in advanced molecular Biology techniques.<br />No of Post: 01<br />How To Apply: Walk-in-Interviews will be held at ICAR-National Research Centre for Orchids,Pakyong 737106, Sikkim for the post of 01 (One) Junior Research Fellow and 01 (One) Project Attendant under Project ‘DBT’s Twinning programme for the NE’ titled “Assessment of chemical and genetic divergence of some fragrant orchids of north-east India for sustainable improvement of community livelihood” as indicated below. The appointment will be on contractual basis and the incumbents shall not have any claim for regular appointment in ICAR.</p>

<p>Details will be available at: http://nrcorchids.nic.in/Employments/Vacancy%20-%20JRF.pdf</p>
]]></description>
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