BOL: Related items

MEGAN6

Neel — Mon, 25 Jul 2016 05:45:22 -0500

Microbiome analysis using a single application

MEGAN6 is a comprehensive toolbox for interactively analyzing microbiome data. All the interactive tools you need in one application.

Taxonomic analysis using the NCBI taxonomy or a customized taxonomy such as SILVA
Functional analysis using InterPro2GO, SEED, eggNOG or KEGG
Bar charts, word clouds, Voronoi tree maps and many other charts
PCoA, clustering and networks
Supports metadata
MEGAN parses many different types of input

Why use MEGAN6?

The software is:

Easy to use. MEGAN6 is a single application and all features are available through menus, toolbars and graphics. No scripting skills required.
Powerful. MEGAN6 allows you to work with hundreds of samples containing hundreds of millions of sequencing reads. Blast-like analysis can be performed using DIAMOND.
Comprehensive. MEGAN6 offers a large range of analysis tools, and is under active development.

Address of the bookmark: https://ab.inf.uni-tuebingen.de/software/megan6

Research Associate (Computer Sciences) recruitment in National Bureau of Plant Genetic Resources

Thu, 28 Jul 2016 04:39:13 -0500

Research Associate (Computer Sciences) recruitment in National Bureau of Plant Genetic Resources

Project: Indo-UK Centre for improvement of Nitrogen use efficiency in wheat Dr. Soma S. Marla, Pr. Scientist (Bioinformatics), Division of Genomic Resources, ICAR, NBPGR, ND.

Qualification: Ph.D. Degree in Computer Sciences/Bioinformatics OR 1. First class Master’s degree in any discipline of Plant Sciences with specialization in Computer Sciences/ Bioinformatics having 1st division or 60% marks or equivalent overall grade point average with at least two years of research experience as evidenced from Fellowship/ Associate ship. 2. NET qualification is essential for the candidates with 3+2 years B.Sc.+ M.Sc. Desirable: Demonstrated experience & skills in database design, management, UNIX OS, in NGS data analysis. Experience substantiated by publications of high quality will be preferred.

No.of Post: 1

Pay Scale: Rs. 40,000 (Ph.D)/ Rs + 30 % HRA; Rs.38,000 ( Masters Degree 0 + 30 % HRA).

Age Limit : below 40 years for RA position
How to apply
Applicants for RA post should send their complete CV (Advance copy of the application may be sent by email to :soma.marla@icar.gov.in or ssmarl@yahoo.com, should enclose the copy of the research publications; one page summary of their achievement relevant to the post applied for; and should enclose two reference letters (one must be from the person with whom worked latest). Shortlisted candidate will be intimated for interview by email.

The candidates who wish to attend the walk-in interview are requested to bring with them five copies of the CV (one copy with photograph) as per the format given below. Also, the candidates should bring the original documents such as degree certificates, marks sheets, publications, thesis, experience certificate etc. for verification.

Date of Interview: 17.8.2016.

More at http://www.nbpgr.ernet.in/Downloadfile.aspx?EntryId=7133

Genome STRiP

Neel — Tue, 06 Sep 2016 03:58:19 -0500

Genome STRiP (Genome STRucture In Populations) is a suite of tools for discovering and genotyping structural variations using sequencing data. The methods are designed to detect shared variation using data from multiple individuals.

Genome STRiP looks both across and within a set of sequenced genomes to detect variation. The methods are adaptive and support heterogeneous data sets, including variations in sequencing depth, read lengths and mixtures of paired and single-end reads. A minimum of 20 to 30 genomes are required to get acceptable results, but the method gains power across genomes and processing more genomes provide better results.

To run discovery or genotyping on a single sequenced genome or a small set of genomes, you need to call your data against a background population, such as a set of genomes from the 1000 Genomes Project. The background population does not need to be matched to the target individuals.

Address of the bookmark: http://software.broadinstitute.org/software/genomestrip/

Comparison of Short Read De Novo Alignment Algorithms

Rahul Agarwal — Wed, 21 Aug 2013 07:56:01 -0500

Excellent article to introduce different sequencing methods along with tools for de novo assembly of sequencing reads and their relevant references.

Title: Comparison of Short Read De Novo Alignment Algorithms

Author: Nikhil Gopal

Address of the bookmark: http://biochem218.stanford.edu/Projects%202011/Gopal%202011.pdf

WALK-IN INTERVIEW @ JIPMER

Mon, 05 Sep 2016 04:01:13 -0500

Department of Preventive and Social Medicine
, JIPMER, Puducherry –605006

WALK-IN INTERVIEW

JIP/PSM/INDO-US TB/ 2016/

Walk-in-interview for the following vacant posts funded by Department of Biotechnology, Govt.of India for the project entitled “Biomarkers for Risk of Tuberculosis and for Tuberculosis Treatment Failure and Relapse” in the Department of Preventive & Social Medicine, JIPMER, Puducherry.

3. Technical Assistant

MCA/ MSc in Biostatistics/ MSc in Computational Biology from any recognized University @ Rs.23,220 1

Interested candidates may attend the walk-in interview with written screening test on 07, September 2016 at 9.30 A.M in the Dept. of Preventive and Social Medicine, IV Floor, Administrative Block, JIPMER.

The applicants are requested to bring the filled in application form and bio-data with original certificates for verification.

More Info: http://jipmer.edu.in/wp-content/uploads/2016/09/RECRUITEMENTsite-protocol-7.9.2016.pdf

coursera genome assembly tutorial

Jit — Sat, 25 Nov 2017 08:57:25 -0600

Solutions to Coursera Genome Sequencing (Bioinformatics II)

Address of the bookmark: https://github.com/iansealy/coursera-assembly

JRF Bioinformatics at MANIT, India

Thu, 18 Aug 2016 02:48:53 -0500

Advt. No.: Maths./577/2016 Date: 12/08/2016
JRF Bioinformatics Job Position in Maulana Azad National Institute of Technology (MANIT) purely temporary basis
Project Title : “Computational Approach to Study Complex Biological Network of Diseases using Molecular Data”
Essential Qualifications & experience: M.Tech in Bioinformatics/ Computational System biology/Computer Science or M.Sc. in Bio informatics/Biotechnology/Mathematics/Statistics from recognized University/ Institute. Preference will be given to GATE/NET qualified candidates.
No. of Post : 01
Fellowship: INR 12000

How to apply
The duly completed application on prescribed format along with copies of supporting documents must reach to: office of the Dr. Usha Chouhan, Principal Investigator, Department of Mathematics, Bioinformatics & Computer Applications, Maulana Azad National Institute of Technology, Bhopal-462003 on or before 31/08/2016. A soft copy of the application should also be sent to ycchouhan@gmail.com email address of Principal Investigator.

More at http://www.web.manit.ac.in/Year%202016/JRF/walk%20in.pdf

COPE: an accurate k-mer-based pair-end reads connection tool to facilitate genome assembly

Jit — Wed, 06 Dec 2017 02:08:14 -0600

An efficient tool called Connecting Overlapped Pair-End (COPE) reads, to connect overlapping pair-end reads using k-mer frequencies. We evaluated our tool on 30× simulated pair-end reads from Arabidopsis thaliana with 1% base error. COPE connected over 99% of reads with 98.8% accuracy, which is, respectively, 10 and 2% higher than the recently published tool FLASH. When COPE is applied to real reads for genome assembly, the resulting contigs are found to have fewer errors and give a 14-fold improvement in the N50 measurement when compared with the contigs produced using unconnected reads.

Address of the bookmark: ftp://ftp.genomics.org.cn/pub/cope

vcfR

Archana Malhotra — Fri, 19 Aug 2016 07:38:24 -0500

Most variant calling pipelines result in files containing large quantities of variant information. The variant call format (vcf) is an increasingly popular format for this data. The format of these files and their content is discussed in the vignette ‘vcf data.’ These files are typically intended to be post-processed (i.e., filtered) as an attempt to remove false positives or otherwise problematic sites. The R package vcfR provides tools to facilitate this filtering as well as to visualize the effects of choices made during this process.

Address of the bookmark: https://cran.r-project.org/web/packages/vcfR/vignettes/visualization_1.html

3d-dna: 3D de novo assembly (3D DNA) pipeline

Jit — Thu, 28 Dec 2017 10:09:37 -0600

This code is designed to enable anyone to reproduce the Hs2-HiC and the AaegL4 genomes reported in: Dudchenko et al., De novo assembly of the Aedes aegypti genome using Hi-C yields chromosome-length scaffolds. Science, 2017.

Unless otherwise noted, all terminology below is consistent with this paper, and all references to figures and tables in this readme refer to this paper. Specifically, some of the terminology used below is outlined in Figure S2. The assembly procedure is described in detail in the Supporting Online Materials, specifically in the section labelled “Pipeline description”.

In addition, the pipeline uses tools and methods from Juicer (Durand & Shamim et al., Cell Systems, 2016) and Juicebox (Durand & Robinson et al., Cell Systems, 2016), as well as additional dependencies noted below.

Feel free to post your questions and comments at: http://www.aidenlab.org/forum.html

http://aidenlab.org/documentation.html

Address of the bookmark: https://github.com/theaidenlab/3d-dna