BOL: Related items

JRF Bioinformatics at DPU, India

Fri, 05 Aug 2016 03:06:26 -0500

Advertisement for position of “JRF (Junior Research Fellow)” on DST research project “Molecular modeling and docking studies on Deguelin and its derivatives with cell cycle arrest, apoptosis and anti-angiogenesis pathway proteins in cancer cell signaling pathway”

Applications are invited on plain paper from eligible candidates along with biodata and copies of certificates in support of age, qualification and experience for the following position:

Particulars Description

1. Position & No. JRF (Junior Research Fellow) 01

2. Title of the Project Molecular modeling and docking studies on Deguelin and its derivatives with cell cycle arrest, apoptosis and anti-angiogenesis pathway proteins in cancer cell signaling pathway

3. Tenure 3 years

4. Investigator Dr. K. V.Swamy

5. Institute Dr. D. Y. Patil Biotechnology and Bioinformatics Institute, Tathawade, Pune 411033.

5. Qualifications/Eligibility

Essential: NET (National Eligibility Test) qualified M. Sc Bioinformatics/ M. Tech Bioinformatics/M. Sc Biotechnology/M. Tech Biotechnology or Graduate degree in Professional course with NET qualification or Post graduation degree in professional course

The following examinations conducted by various Central Government Departments/Agencies are considered as National Eligibility Test (NET).

1. CSIR-UGC-LS
2. GATE (Graduate Aptitude Test in Engineering)
3. JAM (Joint Admission Test)
4. GPAT (Graduate Pharmacy Aptitude Test)
5. BET(Biotechnology Eligibility Test)
6. BINC(Bioinformatics National Consortium)
7. JEST( Joint Entrance Screening Test)
8. JGEEBILS(Joint Graduate Entrance Examination for Biology & Interdisciplinary Life Sciences)
9. NBHM Ph.D scholarship Screening Test
10. ICMR- JRF Entrance Examination
11. AICE (ICAR-All India competitive Examination )
(For all above examinations valid score considered at the time of interview)

Desirable: Knowledge and skills in Bioinformatics tools/ softwares

6. Monthly Emoluments Rs.25,000/ (As per DST-SERB rules)

7. Last date for submission of prescribed application 20/08/2016

Kindly send your applications to “Dr. K. V. Swamy, Asst.Professor, Dr. D. Y. Patil Biotechnology & Bioinformatics Institute, Survey No. 87/88, Mumbai-Pune Express Way, Tathawade, Pune - 411033, Maharashtra, India”. Highlight the envelope with “Application for post of JRF (Junior Research Fellow)”.

Note: No TA/DA will be paid for attending the interview.

Advertisement: http://careers.dpu.edu.in/Biotech.aspx

RePS: Repeat-masked Phrap with scaffolding, a WGS sequence assembler

Jit — Sat, 04 Jan 2020 01:08:09 -0600

RePS (Repeat-masked Phrap with scaffolding), a WGS sequence assembler, that explicitly identifies exact kmer repeats from the shotgun data and removes them prior to the assembly. The established software Phrap is used to compute meaningful error probabilities for each base. Clone-end-pairing information is used to construct scaffolds that order and orient the contigs. The updated version of RePS incorporates some of the ideas introduced by Phusion on clustering

More at

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC186573/

Address of the bookmark: ftp://ftp.genomics.org.cn/pub/ricedb/Tools/RePS/RePS-IBM-AIX.tar.gz

Research Associate Bioinformatics recruitment in ICGEB, New Delhi

Tue, 16 Aug 2016 03:38:05 -0500

Research Associate Bioinformatics recruitment in ICGEB, New Delhi

Project :“Genetic Transformation and Development of Elite Transgenic Maize (Zea mays L.) for Biotic and Abiotic Stresses Tolerance”.

Qualification: Ph.D. degree in:Biotechnology/Bioinformatics/Biochemistry/Plant Molecular Biology/Plant Physiology/Botany or any related area with evidence of prior experience in maize transformation.

Additional experience in plant transformation of any cereal crop would be preferable.

The appointment would initially be for one year.

How to apply
Interested applicants should send their detailed CV including brief synopsis regarding the previous research experience (along withcontact email address by email) to: Dr. Tanushri Kaul (tanushri@icgeb.res.in). Group Leader, Nutritional Improvement of Crops Group, International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi-110 067.

Closing date for applications: 22 August 2016.

More at http://www.icgeb.org/vacancies.html

Senior Manager (Bioinformatics Operations) at RGCB, India

Fri, 19 Aug 2016 04:37:17 -0500

Senior Manager (Bioinformatics Operations)
Rajiv Gandhi Centre for Biotechnology (RGCB) - Thiruvananthapuram, Kerala
₹15,600 - ₹39,100 a month
Rajiv Gandhi Centre for Biotechnology (RGCB) invites applications for the following positions from Indian citizens with prescribed qualifications

Senior Manager (Bioinformatics Operations)
No. of posts: 01 (General)
Pay Scale: PB-3 15600-39100 + Grade Pay Rs.6600/-
Min. Qualification: PhD in Bioinformatics, , Life Sciences or Computer Science applied to biological questions.
A Min. 5 years of experience
Age limit: 40 years as on August 01, 2016

More at http://www.rgcb.res.in/positions.php

Omega2: metagenome assembly pipeline

Jit — Mon, 10 Jul 2017 05:56:07 -0500

Omega found overlaps between reads using a prefix/suffix hash table. The overlap graph of reads was simplified by removing transitive edges and trimming short branches. Unitigs were generated based on minimum cost flow analysis of the overlap graph and then merged to contigs and scaffolds using mate-pair information. In comparison with three de Bruijn graph assemblers (SOAPdenovo, IDBA-UD and MetaVelvet), Omega provided comparable overall performance on a HiSeq 100-bp dataset and superior performance on a MiSeq 300-bp dataset. In comparison with Celera on the MiSeq dataset, Omega provided more continuous assemblies overall using a fraction of the computing time of existing overlap-layout-consensus assemblers. This indicates Omega can more efficiently assemble longer Illumina reads, and at deeper coverage, for metagenomic datasets.

Address of the bookmark: http://omega.omicsbio.org/

Genome assembly stats plotting

Jit — Wed, 28 Feb 2018 03:45:39 -0600

A de novo genome assembly can be summarised b

y a number of metrics, including:

Overall assembly length
Number of scaffolds/contigs
Length of longest scaffold/contig
Scaffold/contig N50 and N90Assembly base composition, in particular percentage GC and percentage Ns
CEGMA completeness
Scaffold/contig length/count distribution

assembly-stats supports two widely used presentations of these values, tabular and cumulative length plots, and introduces an additional circular plot that summarises most commonly used assembly metrics in a single visualisation. Each of these presentations is generated using javascript from a common (JSON) data structure, allowing toggling between alternative views, and each can be applied to a single or multiple assemblies to allow direct comparison of alternate assemblies.

Tabular presentation allows direct comparison of exact values between assemblies, the limitations of this approach lie in the necessary omission of distributions and the challenge of interpreting ratios of values that may vary by several orders of magnitude.

Address of the bookmark: https://github.com/rjchallis/assembly-stats

HapCUT2: robust and accurate haplotype assembly for diverse sequencing technologies

Jit — Tue, 15 May 2018 07:35:26 -0500

HapCUT2 is a maximum-likelihood-based tool for assembling haplotypes from DNA sequence reads, designed to "just work" with excellent speed and accuracy. We found that previously described haplotype assembly methods are specialized for specific read technologies or protocols, with slow or inaccurate performance on others. With this in mind, HapCUT2 is designed for speed and accuracy across diverse sequencing technologies, including but not limited to: NGS short reads (Illumina HiSeq) clone-based sequencing (Fosmid or BAC clones) SMRT reads (PacBio) Oxford Nanopore reads 10X Genomics Linked-Reads proximity-ligation (Hi-C) reads high-coverage sequencing (>40x coverage-per-SNP) using above technologies combinations of the above technologies (e.g. scaffold long reads with Hi-C reads) See below for specific examples of command line options and best practices for some of these technologies. NOTE: At this time HapCUT2 is for diploid organisms only. VCF input should contain diploid variants. If you use HapCUT2 in your research, please cite: Edge, P., Bafna, V. & Bansal, V. HapCUT2: robust and accurate haplotype assembly for diverse sequencing technologies. Genome Res. gr.213462.116 (2016). doi:10.1101/gr.213462.116

Address of the bookmark: https://github.com/vibansal/HapCUT2

SWALO: Scaffolding with assembly likelihood optimization

Jit — Wed, 20 Jun 2018 02:45:16 -0500

SWALO (scaffolding with assembly likelihood optimization) is a method for scaffolding based on likelihood of genome assemblies computed using generative models for sequencing. Please email your questions, comments, suggestions, and bug reports to atif.bd@gmail.com.

Address of the bookmark: https://atifrahman.github.io/SWALO/

SRF Bioinformatics at Bose Institute, India

Thu, 29 Sep 2016 09:39:13 -0500

Advt. No. S/DPB(CB)/17/2016-17
Junior Research Assistant/ Senior Research Assistant Job vacancies in Bose Institute on temporary basis
Project Title : “Genome wide transcriptome analysis to identity MYMIV-stress related genomic resources of blackgram”
Junior Research Assistant
Qualification : Good academic record in B.Sc. and M.Sc. in Botany or Biotechnology from reputed Universities. Must have good practical hand, this should be certified by two Senior/reputed PG teachers on a scale of 10.
Desirable : Preference will be given to those who already have some research experience (for at least 2 months).
Consolidated Pay : Rs. 16,400/- p.m.
Age Limit : Below 28 years (relaxable in case of SC/ST/OBC/Women candidates only as per rules).
Senior Research Assistant
Qualification : B.Sc. in Biotechnology and M.Sc. in Biotechnology or Bioinformatics. Hands on training in Bioinformatics. At least two Research Publications with Bioinformatics components.
Desirable : Preference will be given to the candidates who have previous experience in Next Gen Sequencing data analysis of genomic/transcriptome/miRNA.
Consolidated Pay : Rs. 18,700/- p.m.
Age Limit : Below 30 years (relaxable in case of SC/ST/OBC/Women candidates only as per rules).

http://www.boseinst.ernet.in/advertisement.html

Converting a VCF into a FASTA given some reference !

Jit — Fri, 20 Jul 2018 10:03:53 -0500

Samtools/BCFtools (Heng Li) provides a Perl script vcfutils.pl which does this, the function vcf2fq (lines 469-528)

This script has been modified by others to convert InDels as well, e.g. this by David Eccles

./vcf2fq.pl -f <input.fasta> <all-site.vcf> > <output.fastq>

https://github.com/gringer/bioinfscripts/blob/master/vcf2fq.pl

https://github.com/lh3/samtools/blob/master/bcftools/vcfutils.pl