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	<title><![CDATA[BOL: Related items]]></title>
	<link>https://bioinformaticsonline.com/related/27035?offset=770</link>
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	<description><![CDATA[]]></description>
	
	<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/38210/skesa-strategic-k-mer-extension-for-scrupulous-assemblies</guid>
	<pubDate>Wed, 14 Nov 2018 04:45:41 -0600</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/38210/skesa-strategic-k-mer-extension-for-scrupulous-assemblies</link>
	<title><![CDATA[SKESA: strategic k-mer extension for scrupulous assemblies]]></title>
	<description><![CDATA[<p><span>SKESA is a DeBruijn graph-based de-novo assembler designed for assembling reads of microbial genomes sequenced using Illumina. Comparison with SPAdes and MegaHit shows that SKESA produces assemblies that have high sequence quality and contiguity, handles low-level contamination in reads, is fast, and produces an identical assembly for the same input when assembled multiple times with the same or different compute resources. </span></p>
<p><span>Source code for SKESA is freely available at&nbsp;</span><span><a href="https://github.com/ncbi/SKESA/releases"><span>https://github.com/ncbi/SKESA/releases</span></a></span><span>.</span></p>
<p>Research Paper&nbsp;@ <a href="https://genomebiology.biomedcentral.com/articles/10.1186/s13059-018-1540-z">Link</a></p>
<p><span><span>SKESA algorithm are as follows:</span><br></span></p>
<p><span><img src="https://media.springernature.com/lw785/springer-static/image/art%3A10.1186%2Fs13059-018-1540-z/MediaObjects/13059_2018_1540_Fig4_HTML.png" alt="image" width="785" height="984" style="border: 0px; border: 0px;"></span></p><p>Address of the bookmark: <a href="https://github.com/ncbi/SKESA/releases" rel="nofollow">https://github.com/ncbi/SKESA/releases</a></p>]]></description>
	<dc:creator>Jit</dc:creator>
</item>
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	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/29410/entrez-direct-e-utilities-on-the-unix-command-line</guid>
	<pubDate>Wed, 19 Oct 2016 08:06:24 -0500</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/29410/entrez-direct-e-utilities-on-the-unix-command-line</link>
	<title><![CDATA[Entrez Direct: E-utilities on the UNIX Command Line]]></title>
	<description><![CDATA[<p>Entrez Direct (EDirect) is an advanced method for accessing the NCBI's suite of interconnected databases (publication, sequence, structure, gene, variation, expression, etc.) from a UNIX terminal window. Functions take search terms from command-line arguments. Individual operations are combined to build multi-step queries. Record retrieval and formatting normally complete the process.</p>
<p>EDirect also provides an argument-driven function that simplifies the extraction of data from document summaries or other results that are returned in structured XML format. This can eliminate the need for writing custom software to answer ad hoc questions. Queries can move seamlessly between EDirect commands and UNIX utilities or scripts to perform actions that cannot be accomplished entirely within Entrez.</p><p>Address of the bookmark: <a href="https://www.ncbi.nlm.nih.gov/books/NBK179288/" rel="nofollow">https://www.ncbi.nlm.nih.gov/books/NBK179288/</a></p>]]></description>
	<dc:creator>Anjana</dc:creator>
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<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/38526/versatile-genome-assembly-evaluation-with-quast-lg</guid>
	<pubDate>Fri, 21 Dec 2018 22:06:31 -0600</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/38526/versatile-genome-assembly-evaluation-with-quast-lg</link>
	<title><![CDATA[Versatile genome assembly evaluation with QUAST-LG]]></title>
	<description><![CDATA[<p>QUAST-LG is an extension of&nbsp;<a href="http://cab.spbu.ru/software/quast/">QUAST</a>&nbsp;intended for evaluating large-scale genome assemblies (up to mammalian-size).</p>
<p>QUAST-LG&nbsp;is included in the QUAST&nbsp; package starting from version 5.0.0 (<a href="https://sourceforge.net/projects/quast/files/latest/download?source=files">download the latest release</a>). Run QUAST as usual and do not forget to add&nbsp;<span>‐‐large</span>&nbsp;option to your command!</p>
<p>A short list of the new features (see&nbsp;<a href="http://cab.spbu.ru/files/quast/latest-docs/CHANGES.txt">CHANGES</a>&nbsp;for all):</p>
<ul>
<li>Significant speedup achieved by both&nbsp;use of new fast aligner (<a href="https://github.com/lh3/minimap2">minimap2</a>) and the refactoring of alignment analyzing&nbsp;modules</li>
<li>New k-mer-based completeness and correctness metrics</li>
<li>BUSCO added for enhanced reference-free analysis</li>
<li>The concept of upper bound&nbsp;assembly (theoretical limits on the assembly&nbsp;completeness and&nbsp;contiguity for a given genome and set of reads)</li>
</ul><p>Address of the bookmark: <a href="http://cab.spbu.ru/software/quast-lg/" rel="nofollow">http://cab.spbu.ru/software/quast-lg/</a></p>]]></description>
	<dc:creator>Jit</dc:creator>
</item>
<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/blog/view/38765/list-of-tools-frequently-used-while-genome-assembly</guid>
	<pubDate>Tue, 22 Jan 2019 09:39:02 -0600</pubDate>
	<link>https://bioinformaticsonline.com/blog/view/38765/list-of-tools-frequently-used-while-genome-assembly</link>
	<title><![CDATA[List of tools frequently used while genome assembly]]></title>
	<description><![CDATA[<h4>List of tools frequently used while genome assembly:</h4><p>I have used the following assemblers</p><ul>
<li><a href="http://bioinf.spbau.ru/spades">Spades</a>&nbsp;(v. 3.10.1)</li>
<li><a href="http://canu.readthedocs.io/en/stable/index.html">CANU</a>&nbsp;(v. 1.6)</li>
<li><a href="https://github.com/rrwick/Unicycler">Unicycler&nbsp;</a>(v. v0.4.1)</li>
<li><a href="https://github.com/lh3/miniasm">Miniasm</a>&nbsp;(v. 0.2-r137-dirty)</li>
</ul><p>I have used the following mappers</p><ul>
<li><a href="https://github.com/lh3/minimap2">minimap2</a>&nbsp;(v.&nbsp;2.0rc1-r232)</li>
<li><a href="https://github.com/lh3/minimap">minimap&nbsp;</a>(v. 0.2-r124-dirty)</li>
<li><a href="https://github.com/lh3/bwa">bwa</a>&nbsp;(v.&nbsp;0.7.12-r1039)</li>
</ul><p>I have used the following polishing tools</p><ul>
<li><a href="https://github.com/isovic/racon">Racon</a>&nbsp;(v. not available)</li>
<li><a href="https://github.com/broadinstitute/pilon">Pilon</a>&nbsp;(v. 1.18)</li>
<li><a href="https://github.com/jts/nanopolish">Nanopolish</a>&nbsp;(v. 0.8.3)</li>
</ul><p>I have used the following tools to assess genome assembly characteristics</p><ul>
<li><a href="https://github.com/chjp/ANI">ANI.pl</a>&nbsp;(https://github.com/chjp/ANI)</li>
<li><a href="http://ecogenomics.github.io/CheckM/">CheckM</a>&nbsp;(v. 1.0.7)</li>
<li><a href="https://github.com/tseemann/prokka">Prokka</a>&nbsp;(v. 1.12)</li>
<li><a href="http://bioinf.spbau.ru/en/quast">QUAST</a>&nbsp;(v. 2.3)</li>
<li><a href="http://mummer.sourceforge.net/">mummer&nbsp;</a>(v. not available)</li>
</ul><p>If you have any ideas or superior tools we have missed please let us know in the comments.</p>]]></description>
	<dc:creator>BioStar</dc:creator>
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	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/29578/plink2</guid>
	<pubDate>Thu, 27 Oct 2016 11:24:45 -0500</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/29578/plink2</link>
	<title><![CDATA[PLINK2]]></title>
	<description><![CDATA[<p><span>This is a comprehensive update to Shaun Purcell's&nbsp;</span><a href="http://pngu.mgh.harvard.edu/~purcell/plink/">PLINK</a><span>&nbsp;command-line program, developed by&nbsp;</span><a href="mailto:chrchang@alumni.caltech.edu">Christopher Chang</a><span>&nbsp;with support from the&nbsp;</span><a href="http://www.niddk.nih.gov/">NIH-NIDDK</a><span>'s Laboratory of Biological Modeling, the&nbsp;</span><a href="http://research.mssm.edu/statgen/">Purcell Lab</a><span>&nbsp;at Mount Sinai School of Medicine, and others. (</span><a href="https://www.cog-genomics.org/plink2/#new">What's new?</a><span>) (</span><a href="https://www.cog-genomics.org/plink2/credits">Credits.</a><span>) (</span><a href="http://www.gigasciencejournal.com/content/4/1/7">Methods paper.</a><span>)</span></p><p>Address of the bookmark: <a href="https://www.cog-genomics.org/plink2/" rel="nofollow">https://www.cog-genomics.org/plink2/</a></p>]]></description>
	<dc:creator>Jit</dc:creator>
</item>
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	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/40099/contiguator</guid>
	<pubDate>Fri, 04 Oct 2019 01:27:58 -0500</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/40099/contiguator</link>
	<title><![CDATA[CONTIGuator !]]></title>
	<description><![CDATA[<p><span>CONTIGuator is a Python script for Linux environments whose purpose is to speed-up the bacterial genome assembly process and to obtain a first insight of the genome structure using the well-known artemis comparison tool (ACT).</span></p>
<p>&nbsp;</p><p>Address of the bookmark: <a href="https://sourceforge.net/projects/contiguator/" rel="nofollow">https://sourceforge.net/projects/contiguator/</a></p>]]></description>
	<dc:creator>BioStar</dc:creator>
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<item>
  <guid isPermaLink='true'>https://bioinformaticsonline.com/opportunity/view/29624/information-officer-at-iiar-gujarat</guid>
  <pubDate>Fri, 04 Nov 2016 05:19:13 -0500</pubDate>
  <link></link>
  <title><![CDATA[Information Officer at IIAR, Gujarat]]></title>
  <description><![CDATA[
<p>Walk in interview at 10.30 am on Nov 11th, 2016 for the following position at Distributed Information Sub-Centre (DISC) established by Dept. Of Biotechnology, Govt. of India at Indian Institute of Advanced Research, Gandhinagar, Gujarat</p>

<p>Position (scale), qualifications and experience</p>

<p>1. Information Officer (Rs 8000-275-13500): one post <br />Qualifications and experience: MCA, Post-Graduate in any field of biosciences, bioinformatics with at least two years of experience in working in a bioinformatics setup and good knowledge of linux operating system and computer networking.</p>

<p>General terms and conditions: <br />1. The above engagements is presently till March 31st, 2017. However on extension of project grant and satisfactory performance of the candidate, your services can be extended beyond March 31st, 2017 based on the terms and conditions of extension of project grant.</p>

<p>2. It is not an appointment with the institute and will not confer any right to the incumbent to any claim implicit or explicit on any position.</p>

<p>3.No TA/DA will be paid for attending the interview. Outstation candidates have to make their own arrangement for their stay.</p>

<p>4.Candidates, appearing in the walk-in interview are requested to bring the hard copy of application addressed to Dr. Anju Pappachan with latest photograph, CV mentioning qualifications, work experience and name of two referees and one page write up as to why you would like to join the project.</p>

<p>Address: <br />Dr. Anju Pappachan <br />Indian Institute of Advanced Research (IIAR), <br />University  and Institute of Advanced Research, <br />The Puri Foundation for Education in India, <br />Koba Institutional Area, <br />Gandhinagar- 382 007, Gujarat, India. Contact no. 079-30514152 <br />e-mail- anju@iiar.res.in</p>
]]></description>
</item>
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	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/40856/3d-de-novo-assembly-3d-dna-pipeline</guid>
	<pubDate>Sun, 02 Feb 2020 13:41:55 -0600</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/40856/3d-de-novo-assembly-3d-dna-pipeline</link>
	<title><![CDATA[3D de novo assembly (3D DNA) pipeline]]></title>
	<description><![CDATA[<p>For a detailed description of the pipeline and how it integrates with other tools designed by the Aiden Lab see&nbsp;<a href="http://aidenlab.org/assembly/manual_180322.pdf">Genome Assembly Cookbook</a>&nbsp;on&nbsp;<a href="http://aidenlab.org/assembly">http://aidenlab.org/assembly</a>.</p>
<p>For the original version of the pipeline and to reproduce the Hs2-HiC and the AaegL4 genomes reported in&nbsp;<a href="http://science.sciencemag.org/content/356/6333/92">(Dudchenko et al.,&nbsp;<em>Science</em>, 2017)</a>&nbsp;see the&nbsp;<a href="https://github.com/theaidenlab/3d-dna/tree/745779bdf64db6e55bddb70c24e9b58825938c33">original commit</a>.</p>
<p>For the detailed description of the merge section see&nbsp;<a href="https://github.com/theaidenlab/AGWG-merge">https://github.com/theaidenlab/AGWG-merge</a>.</p><p>Address of the bookmark: <a href="https://github.com/theaidenlab/3d-dna" rel="nofollow">https://github.com/theaidenlab/3d-dna</a></p>]]></description>
	<dc:creator>Jit</dc:creator>
</item>

<item>
  <guid isPermaLink='true'>https://bioinformaticsonline.com/opportunity/view/29644/junior-research-fellow-at-rajiv-gandhi-centre-for-biotechnology-thiruvananthapuram</guid>
  <pubDate>Mon, 07 Nov 2016 10:27:06 -0600</pubDate>
  <link></link>
  <title><![CDATA[Junior Research Fellow at Rajiv Gandhi Centre for Biotechnology, Thiruvananthapuram]]></title>
  <description><![CDATA[
<p>Adv. # 22/ 2016<br />Applications are invited from suitable candidates for one position of Junior Research Fellow in a DST funded bioinformatics research project entitled "Major gene influxes in microbial genome evolution" in the Laboratory of Dr. Shijulal Nelson-Sathi at Rajiv Gandhi Centre for Biotechnology, Thiruvananthapuram.</p>

<p>ESSENTIAL QUALIFICATIONS:<br />We are looking for a motivated candidate with keen interest in bioinformatics and microbial genome evolution. The candidate must have a Master’s Degree in Bioinformatics, Computational Biology, Computer Science, Microbiology, Biology or a related field with good academic record.</p>

<p>DESIRABLE QUALIFICATIONS<br />Hands on research experience on handling next generation sequencing data and phylogenetic reconstruction methods. Excellent programming skills (Perl/Python/Java/Php) and experience in working on Unix/Linux platform is preferred. Furthermore; good knowledge is required in statistics (R/Matlab) and the application of bioinformatics analysis tools.</p>

<p>AGE:<br />Below 28 years as on 15th November, 2016.</p>

<p>EMOLUMENTS:<br />Rs. 25,000 + 20% HRA for NET/GATE qualified and Post Graduate in Professional Degree course qualified candidates and <br />Rs. 12,000/- + 20% HRA for others.</p>

<p>DURATION:<br />Initial appointment will be given for one year and further extension will be based on the performance till termination of the project.<br />Only those fulfilling the above criteria need apply and will be called for interview. In the event of more than 10 candidates being short-listed by screening the applications, a written test will be conducted before the selection interview and only those who are successful in the written test will be interviewed. No TA/ DA will be given for appearing in the interview.</p>

<p>Suitably qualified candidates may send applications in the prescribed format (Download here) with a photograph, a copy of full resume indicating the percentage of Marks obtained and attested photocopies of credentials &amp; experience to reach the undersigned on or before 15th November, 2016. Envelopes must be superscripted with abbreviated title of the project, advertisement number and job title. Selection to the position will not entitle the candidate to any future positions at RGCB (permanent or otherwise). As with all project positions at RGCB, the position will be co terminus with end of the project.</p>
]]></description>
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<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/41207/blobtoolkit-a-toolkit-for-genome-assembly-qc</guid>
	<pubDate>Fri, 21 Feb 2020 00:17:50 -0600</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/41207/blobtoolkit-a-toolkit-for-genome-assembly-qc</link>
	<title><![CDATA[BlobToolKit: A toolkit for genome assembly QC]]></title>
	<description><![CDATA[<p>Filtering raw genomic datasets is essential to avoid chimeric assemblies and to increase the validity of sequence-based biological inference. BlobToolKit extends the BlobTools<span>1</span>/Blobology<span>2</span>&nbsp;approach to simplify interactive and reproducible filtering.</p>
<p>BlobToolKit is comprised of four components:</p>
<ol>
<li><a href="https://blobtoolkit.genomehubs.org/btk-viewer/">BlobToolKit Viewer</a>&nbsp;allows browser-based interactive visualisation and filtering of preliminary or published genomic datasets even for highly fragmented assemblies.</li>
<li><a href="https://blobtoolkit.genomehubs.org/blobtools2/">BlobTools2</a>&nbsp;is a command-line program to convert assemblies and analysis results into datasets that can be further processed using&nbsp;<a href="https://blobtoolkit.genomehubs.org/blobtools2/">BlobTools2</a>&nbsp;and/or visualised in the Viewer.</li>
<li>The&nbsp;<a href="https://blobtoolkit.genomehubs.org/specification/">BlobToolKit Specification</a>&nbsp;features a formal schema and validator for the JSON-based BlobDir format used by&nbsp;<a href="https://blobtoolkit.genomehubs.org/blobtools2/">BlobTools2</a>&nbsp;and the&nbsp;<a href="https://blobtoolkit.genomehubs.org/btk-viewer/">Viewer</a>.</li>
<li>The&nbsp;<a href="https://blobtoolkit.genomehubs.org/pipeline/">BlobToolKit Pipeline</a>&nbsp;is a configurable Snakemake pipeline that automates all steps from retrieving public datasets through running analyses and generating a BlobDir dataset with&nbsp;<a href="https://blobtoolkit.genomehubs.org/blobtools2/">BlobTools2</a>, ready for visualisation in the&nbsp;<a href="https://blobtoolkit.genomehubs.org/btk-viewer/">Viewer</a>.</li>
</ol>
<p>Paper&nbsp;<a href="https://www.biorxiv.org/content/10.1101/844852v1.full.pdf">https://www.biorxiv.org/content/10.1101/844852v1.full.pdf</a></p><p>Address of the bookmark: <a href="https://blobtoolkit.genomehubs.org/" rel="nofollow">https://blobtoolkit.genomehubs.org/</a></p>]]></description>
	<dc:creator>Jit</dc:creator>
</item>

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