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	<title><![CDATA[BOL: Related items]]></title>
	<link>https://bioinformaticsonline.com/related/27110?offset=640</link>
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	<description><![CDATA[]]></description>
	
	<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/43892/choosing-the-right-ngs-sequencing-instrument-for-your-study</guid>
	<pubDate>Wed, 15 Jun 2022 00:37:29 -0500</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/43892/choosing-the-right-ngs-sequencing-instrument-for-your-study</link>
	<title><![CDATA[Choosing the Right NGS Sequencing Instrument for Your Study]]></title>
	<description><![CDATA[<p>The right sequencing instrument for your study depends on your project goal. Setting aside turnaround time and price, it essentially comes down to the numbers of reads and read length you need for your experiment. Below, we've described and compared metrics for each of the instruments available. If you&rsquo;re new to high-throughput sequencing and have questions about how you should design your sequencing run, fill out our&nbsp;<a href="https://genohub.com/ngs-consultation/"><span>free consultation form</span></a>&nbsp;and we'll get in touch with you to help.</p>
<p>More at&nbsp;https://genohub.com/ngs-instrument-guide/</p><p>Address of the bookmark: <a href="https://genohub.com/ngs-instrument-guide/" rel="nofollow">https://genohub.com/ngs-instrument-guide/</a></p>]]></description>
	<dc:creator>Neel</dc:creator>
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	<guid isPermaLink="true">https://bioinformaticsonline.com/pages/view/9204/keep-your-important-ssh-session-running-when-you-disconnect-from-server</guid>
	<pubDate>Sat, 15 Mar 2014 21:39:17 -0500</pubDate>
	<link>https://bioinformaticsonline.com/pages/view/9204/keep-your-important-ssh-session-running-when-you-disconnect-from-server</link>
	<title><![CDATA[Keep Your Important SSH Session Running when You Disconnect from Server !!!]]></title>
	<description><![CDATA[<p>As a Bioinformatician/ Computational biologist we swim in the ocean of genomic/proteomics data, and play with them with an ease. In our day to day simulation, analysis, comparative study we do need to run exhaustive programs, which might take more than a week. In such cases we do need to disconnect from sever in a way that our program/session should not get terminated. To do so there are lots of software, tools such as tmux ( <a href="http://tmux.sourceforge.net/">http://tmux.sourceforge.net/</a>, nohup (<a href="http://ss64.com/bash/nohup.html">http://ss64.com/bash/nohup.html</a>) , byobu (<a href="https://help.ubuntu.com/10.04/serverguide/byobu.html">https://help.ubuntu.com/10.04/serverguide/byobu.html</a>) and other commands (disown -a &amp;&amp; exit), but following are the ones I use the most.</p><p>Screen is like a window manager for your console. It will allow you to keep multiple terminal sessions running and easily switch between them. It also protects you from disconnection, because the screen session doesn&rsquo;t end when you get disconnected.<br /><br />You&rsquo;ll need to make sure that screen is installed on the server you are connecting to. If that server is Ubuntu or Debian, just use this command:<br /><br />sudo apt-get install screen<br /><br />Now you can start a new screen session by just typing screen at the command line. You&rsquo;ll be shown some information about screen. Hit enter, and you&rsquo;ll be at a normal prompt.<br /><br /><strong>To disconnect (but leave the session running)</strong><br /><br />Hit Ctrl + A and then Ctrl + D in immediate succession. You will see the message [detached]<br /><br /><strong>To reconnect to an already running session</strong><br /><br />screen -r<br /><br /><strong>To reconnect to an existing session, or create a new one if none exists</strong><br /><br />screen -D -r<br /><br /><strong>To create a new window inside of a running screen session</strong><br /><br />Hit Ctrl + A and then C in immediate succession. You will see a new prompt.<br /><br /><strong>To switch from one screen window to another</strong><br /><br />Hit Ctrl + A and then Ctrl + A in immediate succession.<br /><br /><strong>To list open screen windows</strong><br /><br />Hit Ctrl + A and then W in immediate succession</p>]]></description>
	<dc:creator>Jitendra Narayan</dc:creator>
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	<guid isPermaLink="true">https://bioinformaticsonline.com/blog/view/44229/common-steps-for-reads-mapping</guid>
	<pubDate>Thu, 09 Mar 2023 02:48:02 -0600</pubDate>
	<link>https://bioinformaticsonline.com/blog/view/44229/common-steps-for-reads-mapping</link>
	<title><![CDATA[Common steps for reads mapping !]]></title>
	<description><![CDATA[<div><div><div><div><div><div><div><div><div><div><p>Mapping reads to a reference genome is an essential step in many types of genomic analysis, such as variant calling and gene expression analysis. Here are some general steps to follow for mapping reads to a genome:</p><ol>
<li>
<p>Choose a read mapper: There are many read mappers available, such as BWA, Bowtie, and HISAT2. Choose a mapper that is appropriate for your type of data and research question.</p>
</li>
<li>
<p>Index the reference genome: Before mapping reads, the reference genome needs to be indexed. This involves creating an index of the genome sequence that allows the mapper to quickly find matches to the reads. Most mappers have their own indexing tools.</p>
</li>
<li>
<p>Prepare the read data: The reads should be in a format that is compatible with the mapper. Most mappers accept FASTQ or BAM files. Depending on the quality of the data, it may need to be filtered or trimmed before mapping.</p>
</li>
<li>
<p>Run the mapper: The mapper is run with the command-line interface or using a graphical user interface. The specific command depends on the mapper being used, but typically involves specifying the input data, reference genome, and output file format.</p>
</li>
<li>
<p>Evaluate the mapping results: After the mapping is complete, the results should be evaluated. This includes assessing the quality of the mapping, such as the mapping rate, the number of mapped reads, and the mapping quality score.</p>
</li>
<li>
<p>Post-processing: Depending on the analysis being performed, post-processing of the mapped reads may be necessary. This can include filtering reads based on quality, removing duplicate reads, and calling variants.</p>
</li>
</ol><p>Overall, mapping reads to a reference genome is a complex process that requires careful consideration of the type of data, the research question, and the specific mapper being used.</p></div></div></div></div></div></div></div></div></div></div>]]></description>
	<dc:creator>BioStar</dc:creator>
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	<guid isPermaLink="true">https://bioinformaticsonline.com/pages/view/9242/check-the-size-of-a-directory-free-disk-space</guid>
	<pubDate>Mon, 17 Mar 2014 02:35:32 -0500</pubDate>
	<link>https://bioinformaticsonline.com/pages/view/9242/check-the-size-of-a-directory-free-disk-space</link>
	<title><![CDATA[Check the Size of a directory &amp; Free disk space.]]></title>
	<description><![CDATA[<p>The amount of databases we bioinformatician deal are just HUGE &hellip; In such cases, we always need to check our server for free spaces etc. I planned this article to explains 2 simple commands that most bioinformatician want to know when they start using Linux / BioLinux. First: Size of a directory (du) and and second: free disk space that exists on your machine (df).</p><p><br /><strong>'du' &ndash; Check the size of a directory</strong></p><p><br />$ du<br />This command ( du) gives you a list of directories that exist in the current working directory along with their sizes in kilobytes (default). The last line of the output gives you the total size of the current directory including its subdirectories. <br /><br />$ du /home/jin1<br />The above command would give you the directory size of the directory /home/david<br /><br />$ du -h<br />The same &ldquo;du&rdquo;command with some flag gives you a better output than the default one. The option '-h' stands for human readable format. Therefore, in order to print the sizes of the files / directories in your desire notation use this time suffixed with a 'k' if its kilobytes and 'M' if its Megabytes and 'G' if its Gigabytes.<br /><br />$ du -ah<br />If you are interested in checking everything present in a folder use above mentioned command. It gives us not only the directories but also all the files that are present in the current directory. The &ldquo;-a&rdquo; flag displays the filenames along with the directory names in the output. <br /><br />$ du -c<br />This gives you a grand total as the last line of the output. So if your directory occupies 30MB the last 2 lines of the output would be 30M.<br /><br />$ du -s<br />Use this command to displays a summary of the directory size. It is the simplest way to know the total size of the current directory.<br /><br />$ du -S<br />This would display the size of the current directory excluding the size of the subdirectories that exist within that directory. So it basically shows you the total size of all the files that exist in the current directory.<br /><br />$ du --exculde=mp3<br />Several times it required to exclude some directory in our size calculation. In such cases the above command would display the size of the current directory along with all its subdirectories, but it would exclude all the files having the given pattern present in their filenames.</p><p><br /><strong>'df' - finding the disk free space / disk usage</strong><br /><br />$ df<br />Hmmm &hellip; now &ldquo;df&rdquo; command is really useful, and I guess you are going to use it over time. Typing the above command, outputs a table consisting of 6 columns. All the columns are very easy to understand. Remember that the 'Size', 'Used' and 'Avail' columns use kilobytes as the unit. The 'Use%' column shows the usage as a percentage which is also very useful.<br /><br />$ df -h<br />Displays the same output as the previous command but the '-h' indicates human readable format. Hence instead of kilobytes as the unit the output would have 'M' for Megabytes and 'G' for Gigabytes.<br /><br />Example: Linux installed on /dev/hda1<br />$ df -h | grep /dev/hda1</p><p><br />All right, this is not the only option to check the sizes and free spaces but there are a few more options that can be used with 'du' and 'df' . I will discuss it later.<br /><br /></p>]]></description>
	<dc:creator>Jitendra Narayan</dc:creator>
</item>
<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/news/view/34711/1mb-long-dna-with-nanopore-technology</guid>
	<pubDate>Tue, 19 Dec 2017 18:49:28 -0600</pubDate>
	<link>https://bioinformaticsonline.com/news/view/34711/1mb-long-dna-with-nanopore-technology</link>
	<title><![CDATA[1mb long DNA with Nanopore technology]]></title>
	<description><![CDATA[<p>The first continuous DNA read of more than a million bases (&gt;1Mb) has been achieved, using Oxford Nanopore sequencing technology. Congratulations to Martin Smith and collaborators! Read more: http://bit.ly/2j5TNCO</p>]]></description>
	<dc:creator>Jit</dc:creator>
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  <guid isPermaLink='true'>https://bioinformaticsonline.com/opportunity/view/9441/jrf-at-gautam-buddha-university</guid>
  <pubDate>Thu, 27 Mar 2014 03:53:57 -0500</pubDate>
  <link></link>
  <title><![CDATA[JRF at Gautam Buddha University]]></title>
  <description><![CDATA[
<p>Gautam Buddha University (GBU) Noida invites applications for the follow posts<br />2014 March Advertisement from Gautam Buddha University (GBU)<br />Junior Research Fellow (JRF)<br />No. of Positions:  01<br />Educational Qualifications:<br />Master degree in any discipline of Life Science with NET qualified or valid GATE score. Desirable Qualification: Preference will be given to candidates having research experience in Bioinformatics<br />Experience:</p>

<p>(details of experience required)<br />Pay Scale:<br />INR Rs.12000/-P.M. + HRA<br />Category:<br />Science and Research Jobs<br />How To Apply:<br />The interested candidates should report for the Interview on 31st<br />March, 2014 at 10:00 am in the Conference Room of Dean, School of Biotechnology, First floor, Gautam Buddha University, Greater<br />Noida. Interested candidates may also send their resume to undersigned by post-mail/e-mail shaktis@gbu.ac.in or shaktisahi@gmail.com. No TA and DA will be paid for appearing for the interview<br />Download Official Notification:</p>

<p>http://www.gbu.ac.in/Recruitment/JRF_advertisement_DSTProject_Shakti_24March14.pdf</p>
]]></description>
</item>
<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/38199/pacasus-correction-of-palindromes-in-long-reads-from-pacbio-and-nanopore</guid>
	<pubDate>Mon, 12 Nov 2018 05:26:48 -0600</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/38199/pacasus-correction-of-palindromes-in-long-reads-from-pacbio-and-nanopore</link>
	<title><![CDATA[Pacasus: Correction of palindromes in long reads from PacBio and Nanopore]]></title>
	<description><![CDATA[<p><br>Tool for detecting and cleaning PacBio / Nanopore long reads after whole genome amplification. Check the poster from the Revolutionizing Next-Generation Sequencing (2nd edition) conference in the source folder:&nbsp;<a href="https://github.com/swarris/Pacasus/blob/master/vib2017.pdf">https://github.com/swarris/Pacasus/blob/master/vib2017.pdf</a>.</p>
<p>The prepint version is found on&nbsp;<a href="http://www.biorxiv.org/content/early/2017/08/09/173872">http://www.biorxiv.org/content/early/2017/08/09/173872</a></p>
<p>It uses the pyPaSWAS framework for sequence alignment (<a href="https://github.com/swarris/pyPaSWAS">https://github.com/swarris/pyPaSWAS</a>)</p><p>Address of the bookmark: <a href="https://github.com/swarris/Pacasus" rel="nofollow">https://github.com/swarris/Pacasus</a></p>]]></description>
	<dc:creator>BioStar</dc:creator>
</item>

<item>
  <guid isPermaLink='true'>https://bioinformaticsonline.com/opportunity/view/9598/junior-research-fellowship-at-gb-pant-university</guid>
  <pubDate>Thu, 03 Apr 2014 12:29:46 -0500</pubDate>
  <link></link>
  <title><![CDATA[Junior Research Fellowship at G.B. PANT UNIVERSITY]]></title>
  <description><![CDATA[
<p>DEPARTMENT OF MOLECULAR BIOLOGY &amp; GENETIC ENGINEERING<br />COLLEGE OF BASIC SCIENCE AND HUMANITIES<br />G.B. PANT UNIVERSITY OF AGRICULTURE AND TECHNOLOGY<br />PANTNAGAR -263145, UTTARAKHAND</p>

<p>No. CBSH/MBGE/356</p>

<p>Subject: Advertisement for the award of Junior Research Fellowship.</p>

<p>Applications are invited for award of one Junior Research Fellowship on a consolidated fellowship of Rs. 12,000/- pm in the project “Bioinformatics Sub-DIC ”, under the Coordinatorship Dr. Anil Kumar. The fellowship is purely temporary and may continue till the duration of the project or maximum three years which ever is earlier. The appointment shall be given on six monthly review basis.</p>

<p>ESSENTIAL QUALIFICATION</p>

<p>M.Sc. Bioinformatics having research experience on In silico experimentation.</p>

<p>Candidates possessing the above qualifications may submit their application on<br />plain paper in the following format to the undersigned latest 18 April, 2014 the interviews will be held on 19 April, 2014 at 11.00 AM in the office of the undersigned. No separate letter for interview will be issued or any TA/DA will be paid for attending the interview.</p>

<p>Advertisement: http://www.gbpuat.ac.in/01042014_18april14_Advertisement%20for%20JRF%20Position,%20BI.pdf</p>
]]></description>
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<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/40544/ngs-bits-short-read-sequencing-tools</guid>
	<pubDate>Thu, 16 Jan 2020 23:14:00 -0600</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/40544/ngs-bits-short-read-sequencing-tools</link>
	<title><![CDATA[ngs-bits - Short-read sequencing tools]]></title>
	<description><![CDATA[<p>Binaries of&nbsp;<em>ngs-bits</em>&nbsp;are available via Bioconda. Alternatively,&nbsp;<em>ngs-bits</em>&nbsp;can be built from sources:</p>
<ul>
<li><span>Binaries</span>&nbsp;for&nbsp;<a href="https://github.com/imgag/ngs-bits/blob/master/doc/install_bioconda.md">Linux/macOS</a></li>
<li>From&nbsp;<span>sources</span>&nbsp;for&nbsp;<a href="https://github.com/imgag/ngs-bits/blob/master/doc/install_unix.md">Linux/macOS</a></li>
<li>From&nbsp;<span>sources</span>&nbsp;for&nbsp;<a href="https://github.com/imgag/ngs-bits/blob/master/doc/install_win.md">Windows</a></li>
</ul><p>Address of the bookmark: <a href="https://github.com/imgag/ngs-bits" rel="nofollow">https://github.com/imgag/ngs-bits</a></p>]]></description>
	<dc:creator>Neel</dc:creator>
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<item>
  <guid isPermaLink='true'>https://bioinformaticsonline.com/opportunity/view/9859/bioinformatics-jrfsrf-position-at-university-of-hyderabad</guid>
  <pubDate>Tue, 15 Apr 2014 20:07:52 -0500</pubDate>
  <link></link>
  <title><![CDATA[Bioinformatics JRF/SRF position at University of Hyderabad]]></title>
  <description><![CDATA[
<p>UNIVERSITY OF HYDERABAD SCHOOL OF LIFE SCIENCES </p>

<p>Applications are invited from qualified individuals for a JRF/SRF position (sponsored by DBT/DST) at Prof. Jagan Pongubala’s laboratory, University of Hyderabad. Dr. Pongubala’s laboratory is investigating the molecular pathways that control the development of innate and adaptive immune cell types utilizing a combination of genetic, molecular and computational approaches.</p>

<p>JRF/SRF</p>

<p>Masters degree in Bioinformatics  (M.Sc./M.Tech.)</p>

<p>Rs. 12,000+HRA<br />Rs. 16,000+HRA</p>

<p>Initial appointment is for one year and  subjected to renewal up to 2 years</p>

<p>Candidates selected for the above position would have a choice to work on computational biology or experimental  biology. Candidates interested to work on computational biology are expected to perform high-throughput sequencing  (NGS) data analysis and should have a strong background in Bioinformatics &amp; Computational Biology, good  programming skills particularly Perl, Python, R and work experience in Linux environment.</p>

<p>Candidates interested to work on experimental biology should have work experience in techniques that are routinely  used in molecular biology and mammalian cell culture. A basic knowledge of bioinformatics is also desired. </p>

<p>Applicants for the above positions should have a Masters degree (M.Tech/M.Sc) with an aggregate marks greater  than 70% or a 7.5 CGPA. Candidates having JRF-fellowship through CSIR/UGC/ICMR/DBT will be encouraged  to enroll into Ph.D. program. The interested candidates having excellent organizational skills and the ability to work  in a team environment with an aspiration to learn new techniques and explore new scientific areas are requested to generate their resume using the link https://cvmkr.com/CV/new#0 and forward to pongubalajagan@gmail.com</p>

<p>Review of applications will begin immediately and continue until the position is filled. Eligible candidates will be called for an interview. No TA/ DA will be paid for attending the interview or at the time of joining the post. Applicants should note that the appointment is purely temporary and subjected to renewal up to three years and there is no Right to Claim for any regular appointment with the University.</p>

<p>Corresponding address: Jagan Pongubala, Ph.D.<br />Department of Animal Sciences<br />School of Life Sciences, Room:S44<br />University of Hyderabad<br />Gachibowli, Hyderabad 500046</p>

<p>Advertisement: https://www.uohyd.ac.in/images/recruitment/jrf-srf_130414.pdf</p>
]]></description>
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