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	<title><![CDATA[BOL: Related items]]></title>
	<link>https://bioinformaticsonline.com/related/27113?offset=720</link>
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	<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/file/view/27455/blosum50-matrix</guid>
	<pubDate>Sat, 21 May 2016 22:12:15 -0500</pubDate>
	<link>https://bioinformaticsonline.com/file/view/27455/blosum50-matrix</link>
	<title><![CDATA[BLOSUM50 Matrix]]></title>
	<description><![CDATA[]]></description>
	<dc:creator>Radha Agarkar</dc:creator>
	<enclosure url="https://bioinformaticsonline.com/file/download/27455" length="2088" type="text/x-fortran" />
</item>
<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/27479/biogps</guid>
	<pubDate>Mon, 23 May 2016 03:15:46 -0500</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/27479/biogps</link>
	<title><![CDATA[BioGPS]]></title>
	<description><![CDATA[<p>A free&nbsp;<em>extensible</em>&nbsp;and&nbsp;<em>customizable</em>&nbsp;<strong>gene annotation portal</strong>, a complete resource for learning about&nbsp;<strong>gene and protein function</strong>.</p>
<p>http://biogps.org/#goto=welcome</p><p>Address of the bookmark: <a href="http://biogps.org/#goto=welcome" rel="nofollow">http://biogps.org/#goto=welcome</a></p>]]></description>
	<dc:creator>Anjana</dc:creator>
</item>
<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/27679/cluego</guid>
	<pubDate>Thu, 02 Jun 2016 09:51:44 -0500</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/27679/cluego</link>
	<title><![CDATA[ClueGO]]></title>
	<description><![CDATA[<p>ClueGO is a Cytoscape plug-in that visualizes the non-redundant biological terms for large clusters of genes in a functionally grouped network and it can be used in combination with GOlorize.</p><p>Address of the bookmark: <a href="http://www.ici.upmc.fr/cluego/" rel="nofollow">http://www.ici.upmc.fr/cluego/</a></p>]]></description>
	<dc:creator>Jit</dc:creator>
</item>

<item>
  <guid isPermaLink='true'>https://bioinformaticsonline.com/opportunity/view/28879/projects-opening-at-nbagr</guid>
  <pubDate>Wed, 24 Aug 2016 04:13:13 -0500</pubDate>
  <link></link>
  <title><![CDATA[Projects opening at NBAGR]]></title>
  <description><![CDATA[
<p>ICAR - NATIONAL BUREAU OF ANIMAL GENETIC RESOURCES</p>

<p>Karnal -132001 (Haryana)</p>

<p>A walk-in-Interview is proposed to be held at National Bureau of Animal Genetic Resources, Karnal (Haryana)-132001 at 10:30 AM on 05.09.2016 for the selection of Three Research Associate &amp; One Young Professional - II as per details given below:</p>

<p>Name of the Scheme / Project: Center for Agricultural Bioinformatics. The post duration is Upto 31.032017 or earlier &amp; Co-terminus with the project.</p>

<p>Research Associate (Three posts)</p>

<p>Date &amp; Time of Interview: 10.30 A.M. on 05.09.2016</p>

<p>Essential Qualifications: PhD degree in any one of discipline/Subject Biotechnology/ Animal Genetics and Breeding/ Biochemistry/ Bioinformatics/Molecular Genetics OR Master’s degree in any one of above mentioned discipline/Subject with 4 years/5 years of Bachelor’s degree having 1st division or 60% marks or equivalent overall grade point average, with at least two years of research experience as evidenced from Fellowship/Associateship</p>

<p>Desirable Qualifications: Experience in Database/Next Generation Sequencing Data analysis for 02 RA posts or working experience in molecular biology, gene expression data analysis, SNP genotyping and sequence data analysis, functional gene characterization for 01 RA post.</p>

<p>Young Professionals II One position</p>

<p>Date &amp; Time of Interview: 10.30 A.M. on 05.09.2016</p>

<p>Essential: B. Tech or M.Tech. in Bioinformatics / Computer Science / Computer Application.</p>

<p>Desirable: Experience in Linux, MySQL, Java, C++/ PHP/ PERL R based data analysis and application development in Bioinformatics.</p>

<p>More Info : http://14.139.252.116/ADvertisementforCabinScheme.pdf</p>
]]></description>
</item>

<item>
  <guid isPermaLink='true'>https://bioinformaticsonline.com/opportunity/view/27927/research-assistant-bioinformatics-at-andhra-university</guid>
  <pubDate>Sat, 18 Jun 2016 18:39:09 -0500</pubDate>
  <link></link>
  <title><![CDATA[Research Assistant Bioinformatics at Andhra University]]></title>
  <description><![CDATA[
<p>Advt. No. AUMLR/BIF/ RA /6-2016 <br />Research Assistant Job Position in Andhra University on temporary basis <br />No. of Post : 01<br />Eligibility : Applicants who have completed their Post Graduate degree in Bioinformatics.<br />Desirable : Undergone traineeship in BIF; at least one publication in Bioinformatics. <br />Stipend : A monthly stipend of Rs. 22,000/- + HRA (HRA is applicable only for NET qualified candidates)<br />How to apply<br />Applications on plain paper, stating the name, address, date of birth, educational qualifications and experiences, and Institute, along with attested photocopies of mark sheets and certificates, should be submitted to K. UMADEVI, Coordinator, BIF Programme, Department of Marine Living Resources, Andhra University, Visakhapatnam-530 003, Andhra Pradesh, on or before 15th July, 2016. </p>

<p>Candidates are required to appear for an interview, with all the necessary certificates in original along with a set of attested copies in the office of the Principal, AU College of Science &amp; Technology, Andhra University, Visakhapatnam. Applications may be sent by Email to andhrauniv.btisnet@nic.in / katruumadevi@gmail.com.</p>
]]></description>
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<item>
  <guid isPermaLink='true'>https://bioinformaticsonline.com/opportunity/view/28272/bioinformatics-openings-at-icgeb-new-delhi-india</guid>
  <pubDate>Mon, 04 Jul 2016 01:04:05 -0500</pubDate>
  <link></link>
  <title><![CDATA[Bioinformatics openings at ICGEB NEW DELHI, INDIA]]></title>
  <description><![CDATA[
<p>Applications are invited for:</p>

<p>ICGEB NEW DELHI, INDIA</p>

<p>Biotechnology research positions</p>

<p>Projects include:</p>

<p>a) protein structure determination<br />b) malaria parasite biology<br />c) genomics and metagenomics<br />d) molecular and cellular biology<br />e) bioinformatics and computational biology</p>

<p>Minimum eligibility for students who have already obtained a MSc:</p>

<p>1) INSPIRE award for PhD<br />2) SPM award for PhD<br />3) CSIR/DBT/DST JRF for PhD</p>

<p>Applicants should submit their curriculum vitae by email to: sb.icgeb@gmail.com by 30 August 2016</p>
]]></description>
</item>

<item>
  <guid isPermaLink='true'>https://bioinformaticsonline.com/opportunity/view/28546/ra-bioinformatics-at-national-bureau-of-fish-genetic-resources</guid>
  <pubDate>Mon, 25 Jul 2016 03:14:06 -0500</pubDate>
  <link></link>
  <title><![CDATA[RA Bioinformatics at  National Bureau of Fish Genetic Resources]]></title>
  <description><![CDATA[
<p>F.No. 1(16)/2016-Admn. (DBT-BBSRC Project)<br />Research Associate /JRF Biotechnology Job vacancies in National Bureau of Fish Genetic Resources on contract basis</p>

<p>Research Associate /01 Post</p>

<p>Essential: Ph.D. in Bioinformatics or 03 years research experience after Post Graduation in Bioinformatics with at least one research paper in Science Citation Indexed (SCI) journals.</p>

<p>Desirable:  The candidate should have at least 1st Division during Graduation and Post Graduation.  Experience in assembly/ analysis/ annotation of genomic/transcriptomic data generated on next generation sequencing platforms and working knowledge on different genomic softwares.  Publications in Relevant Field.</p>

<p>Pay Scale : Rs. 36,000/- +20% HRA </p>

<p>Age: 40 years for male and 45 years for female candidates, as on the date of interview</p>

<p>Junior Research Fellow/ 01 </p>

<p>Essential: Master Degree in Biotechnology/Life Science with Specialization in Molecular Biology with NET qualification. </p>

<p>Desirable:  Research Experience in Molecular Biology. 1st Division during Graduation as well as Post Graduation. Publications in Relevant Field.</p>

<p>Pay Scale: Rs. 25,000/-+ 20% HRA for 1st and 2nd year and Rs. 28,000/-+ 20% HRA for 3rd year</p>

<p>Age: 35 years for male and 40 years for female candidates, as on the date of interview.<br />How to apply<br />A walk-in-interview will be held on 26.07.2016 at 10:00 hrs. at ICAR-National Bureau of Fish Genetic Resources, Lucknow.</p>

<p>More at http://www.nbfgr.res.in/Recruitments.aspx</p>
]]></description>
</item>
<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/33847/omega2-metagenome-assembly-pipeline</guid>
	<pubDate>Mon, 10 Jul 2017 05:56:07 -0500</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/33847/omega2-metagenome-assembly-pipeline</link>
	<title><![CDATA[Omega2: metagenome assembly pipeline]]></title>
	<description><![CDATA[<p><span>Omega found overlaps between reads using a prefix/suffix hash table. The overlap graph of reads was simplified by removing transitive edges and trimming short branches. Unitigs were generated based on minimum cost flow analysis of the overlap graph and then merged to contigs and scaffolds using mate-pair information. In comparison with three de Bruijn graph assemblers (SOAPdenovo, IDBA-UD and MetaVelvet), Omega provided comparable overall performance on a HiSeq 100-bp dataset and superior performance on a MiSeq 300-bp dataset. In comparison with Celera on the MiSeq dataset, Omega provided more continuous assemblies overall using a fraction of the computing time of existing overlap-layout-consensus assemblers. This indicates Omega can more efficiently assemble longer Illumina reads, and at deeper coverage, for metagenomic datasets.</span></p><p>Address of the bookmark: <a href="http://omega.omicsbio.org/" rel="nofollow">http://omega.omicsbio.org/</a></p>]]></description>
	<dc:creator>Jit</dc:creator>
</item>
<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/news/view/28564/dbt-%E2%80%93-bioinformatics-industrial-training-programme-biitp-2016-%E2%80%93-17</guid>
	<pubDate>Wed, 27 Jul 2016 04:09:59 -0500</pubDate>
	<link>https://bioinformaticsonline.com/news/view/28564/dbt-%E2%80%93-bioinformatics-industrial-training-programme-biitp-2016-%E2%80%93-17</link>
	<title><![CDATA[DBT – Bioinformatics Industrial Training Programme (BIITP) 2016 – 17]]></title>
	<description><![CDATA[<p>BIITP is a programme of Department of Biotechnology (DBT), Ministry of Science and Technology, Government of India, managed by Biotech Consortium India Limited (BCIL).The objective of BIITP is to provide an opportunity to bioinformatics students to acquire practical skills and experience by working on projects alongside industry experts as well as to provide an opportunity for the industry to identify potential employees.</p><p><strong>DBT Invites online applications from the bioinformatics&nbsp;students and requisitions from biotech/bioinformatics companies.</strong></p><p><strong>Biotech Industry</strong>&nbsp;:</p><p>Biotech/Bioinformatics companies interested to provide hands on industrial training to the students of Bioinformatics under BIITP may apply online. The companies would have no obligation towards any payments to trainees. The companies would be paid bench fee to cover expenses towards training. Trainees would be provided to companies subject to availability.</p><p><strong>Attn: Bioinformatics Students</strong></p><p>Bioinformatics students interested in training in biotech / bioinformatics companies may apply online.&nbsp;<strong>Stipend of Rs. 10,000/- per month</strong>&nbsp;will be paid to candidates placed for training. The candidates will be selected for training through an interview.</p><p><strong>Eligiblity</strong>&nbsp;:</p><p>a) B.E /B.Tech./M.Sc./M.Tech./Advanced Post Graduate Diploma in Bioinformatics from an Indian recognized university with minimum 55% marks or equivalent grade at highest degree/diploma completed in the year 2015 or 2016 are only eligible to apply.</p><p>b) The Advanced Post Graduate diploma should be of at least one year duration after graduation.</p><p>c)&nbsp; Students whose result of last semester/final year is not declared can also apply mentioning their marks upto the semester/year upto which result declared. The final result with original mark sheet(s) of all the semesters/years will have to be produced at the time of interview.</p><p><strong>Application Procedure</strong>&nbsp;:</p><p>The online application form is available below :</p><p><strong><a href="https://www.biotecnika.org/2016/07/dbt-bioinformatics-industrial-training-programme-biitp-2016-17/?xurl=%3A%2F%2Fwww.bcil.nic.in%2Fbiitp2016-17%2Fregistration1.asp" target="_blank">Application Form For Students (New User)</a></strong></p><p><strong><a href="https://www.biotecnika.org/2016/07/dbt-bioinformatics-industrial-training-programme-biitp-2016-17/?xurl=%3A%2F%2Fwww.bcil.nic.in%2Fbiitp2016-17%2Fregistration.asp%3FT1%3DCompany" target="_blank">Requisition form for companies (New User)</a></strong></p><p><strong><a href="https://www.biotecnika.org/2016/07/dbt-bioinformatics-industrial-training-programme-biitp-2016-17/?xurl=%3A%2F%2Fwww.bcil.nic.in%2Fbiitp2016-17%2Findex1.asp" target="_blank">Already registered User Click Here</a></strong></p><p>The following documents are to be sent to Mr. Manoj Gupta, Manager, Biotech Consortium India Limited, 5th floor, Anuvrat Bhawan, 210, Deen Dayal UpadhyayaMarg, New Delhi-110002.</p><p>More at&nbsp;http://www.bcil.nic.in/biitp2016-17/index.asp</p>]]></description>
	<dc:creator>Jit</dc:creator>
</item>
<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/34416/miniasm-very-fast-olc-based-de-novo-assembler-for-noisy-long-reads</guid>
	<pubDate>Mon, 27 Nov 2017 07:58:49 -0600</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/34416/miniasm-very-fast-olc-based-de-novo-assembler-for-noisy-long-reads</link>
	<title><![CDATA[miniasm: very fast OLC-based de novo assembler for noisy long reads]]></title>
	<description><![CDATA[<p>Miniasm is a very fast OLC-based&nbsp;<em>de novo</em>&nbsp;assembler for noisy long reads. It takes all-vs-all read self-mappings (typically by&nbsp;<a href="https://github.com/lh3/minimap">minimap</a>) as input and outputs an assembly graph in the&nbsp;<a href="https://github.com/pmelsted/GFA-spec/blob/master/GFA-spec.md">GFA</a>&nbsp;format. Different from mainstream assemblers, miniasm does not have a consensus step. It simply concatenates pieces of read sequences to generate the final&nbsp;<a href="http://wgs-assembler.sourceforge.net/wiki/index.php/Celera_Assembler_Terminology">unitig</a>&nbsp;sequences. Thus the per-base error rate is similar to the raw input reads.</p>
<p>So far miniasm is in early development stage. It has only been tested on a dozen of PacBio and Oxford Nanopore (ONT) bacterial data sets. Including the mapping step, it takes about 3 minutes to assemble a bacterial genome. Under the default setting, miniasm assembles 9 out of 12 PacBio datasets and 3 out of 4 ONT datasets into a single contig. The 12 PacBio data sets are&nbsp;<a href="https://github.com/PacificBiosciences/DevNet/wiki/E.-coli-Bacterial-Assembly">PacBio E. coli sample</a>,&nbsp;<a href="http://www.ebi.ac.uk/ena/data/view/ERS473430">ERS473430</a>,&nbsp;<a href="http://www.ebi.ac.uk/ena/data/view/ERS544009">ERS544009</a>,&nbsp;<a href="http://www.ebi.ac.uk/ena/data/view/ERS554120">ERS554120</a>,&nbsp;<a href="http://www.ebi.ac.uk/ena/data/view/ERS605484">ERS605484</a>,&nbsp;<a href="http://www.ebi.ac.uk/ena/data/view/ERS617393">ERS617393</a>,&nbsp;<a href="http://www.ebi.ac.uk/ena/data/view/ERS646601">ERS646601</a>,&nbsp;<a href="http://www.ebi.ac.uk/ena/data/view/ERS659581">ERS659581</a>,&nbsp;<a href="http://www.ebi.ac.uk/ena/data/view/ERS670327">ERS670327</a>,&nbsp;<a href="http://www.ebi.ac.uk/ena/data/view/ERS685285">ERS685285</a>,&nbsp;<a href="http://www.ebi.ac.uk/ena/data/view/ERS743109">ERS743109</a>&nbsp;and a&nbsp;<a href="https://github.com/PacificBiosciences/DevNet/wiki/E.-coli-20kb-Size-Selected-Library-with-P6-C4/ce0533c1d2a957488594f0b29da61ffa3e4627e8">deprecated PacBio E. coli data set</a>. ONT data are acquired from the&nbsp;<a href="http://lab.loman.net/2015/09/24/first-sqk-map-006-experiment/">Loman Lab</a>.</p>
<p>For a&nbsp;<em>C. elegans</em>&nbsp;<a href="https://github.com/PacificBiosciences/DevNet/wiki/C.-elegans-data-set">PacBio data set</a>&nbsp;(only 40X are used, not the whole dataset), miniasm finishes the assembly, including reads overlapping, in ~10 minutes with 16 CPUs. The total assembly size is 105Mb; the N50 is 1.94Mb. In comparison, the&nbsp;<a href="https://github.com/PacificBiosciences/Bioinformatics-Training/wiki/HGAP">HGAP3</a>produces a 104Mb assembly with N50 1.61Mb.&nbsp;<a href="http://lh3lh3.users.sourceforge.net/download/ce-miniasm.png">This dotter plot</a>&nbsp;gives a global view of the miniasm assembly (on the X axis) and the HGAP3 assembly (on Y). They are broadly comparable. Of course, the HGAP3 consensus sequences are much more accurate. In addition, on the whole data set (assembled in ~30 min), the miniasm N50 is reduced to 1.79Mb. Miniasm still needs improvements.</p>
<p>Miniasm confirms that at least for high-coverage bacterial genomes, it is possible to generate long contigs from raw PacBio or ONT reads without error correction. It also shows that&nbsp;<a href="https://github.com/lh3/minimap">minimap</a>&nbsp;can be used as a read overlapper, even though it is probably not as sensitive as the more sophisticated overlapers such as&nbsp;<a href="https://github.com/marbl/MHAP">MHAP</a>&nbsp;and&nbsp;<a href="https://github.com/thegenemyers/DALIGNER">DALIGNER</a>. Coupled with long-read error correctors and consensus tools, miniasm may also be useful to produce high-quality assemblies.</p>
<p>Minimap and miniasm are ultrafast tools for (i) mapping and (ii) assembly. Designed for long, noisy reads, they do not have a correction or consensus step, and therefore the resulting assemblies are contiguous (i.e. long) but very noisy (i.e. full of errors)</p>
<p>We start with an all against all comparison:</p>
<div>
<pre><code>minimap -Sw5 -L100 -m0 -t8 reads.fq reads.fq | gzip -1 &gt; reads.paf.gz
</code></pre>
</div>
<p>Then we can assemble</p>
<div>
<pre><code>miniasm -f reads.fq reads.paf.gz &gt; reads.gfa
</code></pre>
</div>
<p>Convert GFA to FASTA:</p>
<div>
<pre><code>awk <span>'/^S/{print "&gt;"$2"\n"$3}'</span> reads.gfa | fold &gt; reads.fa
</code></pre>
</div>
<p>And then count how many contigs:</p>
<div>
<pre><code>grep <span>"&gt;"</span> reads.fa | wc -l</code></pre>
</div>
<p>&nbsp;</p>
<pre><span><span>#</span> Download sample PacBio from the PBcR website</span>
wget -O- http://www.cbcb.umd.edu/software/PBcR/data/selfSampleData.tar.gz <span>|</span> tar zxf -
ln -s selfSampleData/pacbio_filtered.fastq reads.fq
<span><span>#</span> Install minimap and miniasm (requiring gcc and zlib)</span>
git clone https://github.com/lh3/minimap <span>&amp;&amp;</span> (cd minimap <span>&amp;&amp;</span> make)
git clone https://github.com/lh3/miniasm <span>&amp;&amp;</span> (cd miniasm <span>&amp;&amp;</span> make)
<span><span>#</span> Overlap</span>
minimap/minimap -Sw5 -L100 -m0 -t8 reads.fq reads.fq <span>|</span> gzip -1 <span>&gt;</span> reads.paf.gz
<span><span>#</span> Layout</span>
miniasm/miniasm -f reads.fq reads.paf.gz <span>&gt;</span> reads.gfa</pre><p>Address of the bookmark: <a href="https://github.com/lh3/miniasm" rel="nofollow">https://github.com/lh3/miniasm</a></p>]]></description>
	<dc:creator>Jit</dc:creator>
</item>

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