https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2397507/ 

http://amos.sourceforge.net/wiki/index.php/AMOS

Address of the bookmark: http://amos.sourceforge.net/wiki/index.php/AMOS

ESSENTIAL QUALIFICATION: PH.D. DEGREE IN LIFE SCIENCES / PHYSICAL SCIENCE.

Research Associate for Programme No. –I Specialization in the area of plant molecular biology or plant proteomic study or plant pathogen interaction.
Research Associate for Programme No. –I Specialization in the area of plant / fungal Biotechnology, tissue culture and molecular biology
Research Associate for Programme No. –III Specialization in the area of structural biology and protein crystallography.
Research Associate for Programme No. – V Specialization in the area of microbial physiology (metabolism) or environmental microbiology, with experience in microbial genomics and proteomics.
Research Associate for Programme No. – VII Specialization in the area of Theoretical High Energy Astrophysics or Astroparticle Physics. Proven record of independent research experience in Astrophysical
Radiation Magnetohydrodynamics or Cosmic Ray Astrophysics. Experience in numerical techniques and /or date analysis would be additional advantage.
Associateship : 22,000/- p.m., plus admissible H.R.A. and Medical benefit.
Age: Below 3 Age : Below 35 years (Relaxable in case of SC/ST/OBC/Women candidates only as per rule).
SELECTION PROCEDURE FOR BOSE INSTITUTE- RESEARCH ASSOCIATE POST:

Candidates can apply on or before 13/4/2015.
No detailed information about the selection procedure is mentioned in the recruitment notification.
HOW TO APPLY FOR RESEARCH ASSOCIATE VACANCY IN BOSE INSTITUTE:

Interested and eligible candidates may read the application procedures and instructions carefully before applying through online as well as submitting the hard copy of the same. Candidates those who has submitted their Ph.D. Thesis and can produce Provisional Ph.D. Certificate at the time of Interview may also apply

Ref
Bose Institute Recruitment 2015 – ADVT. NO. : BI/IF/35/2014-15.

The strength of this browser is the ability to navigate an up to date assembly and identify problematic regions and assist in strategizing potential solutions for these issues.

This facilitates the improvement of overall assemblies to a “gold” standard for release as reference genomes

Address of the bookmark: https://geval.sanger.ac.uk/index.html

Protocol Overview / Introduction

In this protocol we discuss and outline the process of de novo assembly for small to medium sized genomes.

https://www.melbournebioinformatics.org.au/tutorials/tutorials/assembly/assembly-protocol/

Address of the bookmark: https://www.melbournebioinformatics.org.au/tutorials/tutorials/assembly/assembly-protocol/

Walk in for Research Asst & Programmer Posts: Enterovirus Research Centre, Mumbai, Indian Council of Medical Research has issued notification for the recruitment of Research Asst & Programmer vacancies on temporary basis for the project entitled “An Atlas of Non-Polio Enterovirus Types Isolated from Cases of Acute Flaccid Paralysis in India”. Eligible candidates may walk in on 20-04-2015 from 10:00 AM to 12:00 Noon. Other details like age limit, educational qualification, how to apply are given below…

Enterovirus Research Centre Mumbai Vacancy Details:
Total No. of Posts: 04
Name of the Posts:
1. Research Assistant: 03 Posts
2. Programmer: 01 Post

Age Limit: Candidates age should below 28 years. Age relaxations are applicable as per rules.

Educational Qualification: Candidates should have M.Sc (1st Class) in Microbiology/ Bioinformatics/ Biotechnology/ Life Science for post 1, BE/ B.Tech/ MCA for post 2.

Selection Process: Candidates are selected based on their performance in interview.

How to Apply: Eligible candidates may attend for interview along with original certificates, CV, attested copies of relevant certificates, one recent passport size photograph duly affixed on right side of application at Enterovirus Research Centre, Mumbai, Indian Council of Medical Research, Haffkine Institute Cmpound, Acharya Donde Marg, Parel, Mumbai-400012 on 20-04-2015 from 10:00 AM to 12:00 Noon.

Important Dates:
Date & Time of Interview: 20-04-2015 from 10:00 AM to 12:00 Noon.
Registration Time: 12:00 Noon.

Address of the bookmark: https://schneebergerlab.github.io/syri/

What is de novo genome assembly?
The method of taking a large number of short DNA sequences and placing them back together to create a reflection of the original chromosomes from which the DNA originated relates to genome assembly. No previous knowledge of the source DNA sequence length, structure or composition is inferred by De novo genome assemblies. The DNA of the target organism is split up into millions of tiny parts and read on a sequencing computer in a genome sequencing experiment. Depending on the sequencing system used, these "reads" range from 20 to 1000 nucleotide base pairs (bp) in length. Usually, length reads of 36 - 150 bp are produced for Illumina style short read sequencing. These reads can be either “single ended” as described above or “paired end.”

Why genome assembly?
In basic research into why and how they live, as well as in applied topics, identifying the DNA sequence of an organism is useful. Awareness of a DNA sequence may be useful in virtually any biological research because of the relevance of DNA to living things. For example, it may be used in medicine to classify, diagnose and eventually improve genetic disorder therapies. Similarly, pathogens study can lead to treatments for infectious diseases.

Raw NGS data
Reads can be saved as a Fasta file as text or in a FastQ file with their attributes. FastQ is the most common read file format since this is what the Illumina sequencing pipeline creates. This will henceforth be the subject of our conversation.

In a nutshell the protocol:
Get the sequence file(s) read from the sequencing machine (s).
Look at the readings - have an idea of what you have and what the standard is like.
If required, raw data cleanup/quality trimming.
Choose an adequate parameter set for assembly.
Assemble the data into scaffolds/contigs.
Examine the assembly performance and determine the efficiency of the assembly.

Read Quality Control:
Check the qualiy with fastQC.
Script
https://bioinformaticsonline.com/snippets/view/42540/install-fastqc-using-conda

Quality trimming/cleanup of read files.
This function trims adapters, barcodes and other contaminants from the reads.
Script
https://bioinformaticsonline.com/snippets/view/42542/trimmomatic-command

Genome Assembly:
The object of this portion of the protocol is to explain the method of assembling the reads trimmed by quality into draft contigs.

spades.py -1 illumina_R1.fastq.gz -2 illumina_R2.fastq.gz --careful --cov-cutoff auto -o result_of_spades_assembly_all_illumina

A significant range of short-read assemblers are available. Everyone with strengths and disadvantages of their own.
Some of the assemblers available include:
Velvet
SOAP-denovo
MIRA
ALLPATHS

Next step is to assess the suitability and what to do with a draft package of contiguous details for the remainder of the study now. Few stuff you can note about the contigs you just created: They're the draft Contigs. Any mis-assemblies can occur.

Mis-assembly checking and assembly metric tools:
QUAST - Quality assessment tool for genome assembly http://bioinf.spbau.ru/quast
Mauve assembly metrics - http://code.google.com/p/ngopt/wiki/How_To_Score_Genome_Assemblies_with_Mauve
InGAP-SV - https://sites.google.com/site/nextgengenomics/ingap and http://ingap.sourceforge.net/
inGAP is also useful for finding structural variants between genomes from read mappings.

Genome finishing tools:
Semi-automated gap fillers:
Gap filler - http://www.baseclear.com/landingpages/basetools-a-wide-range-of-bioinformatics-solutions/gapfiller/

IMAGE (V2) - http://sourceforge.net/apps/mediawiki/image2/index.php?title=Main_Page

Genome visualisers and editors:
Artemis - http://www.sanger.ac.uk/resources/software/artemis/
IGV - http://www.broadinstitute.org/igv/

Automated and semi automated annotation tools:
Prokka - https://github.com/tseemann/prokka
RAST - http://www.nmpdr.org/FIG/wiki/view.cgi/FIG/RapidAnnotationServer
JCVI Annotation Service - http://www.jcvi.org/cms/research/projects/annotation-service/

Frequent command use for the analysis are at:

https://bioinformaticsonline.com/blog/view/38765/list-of-tools-frequently-used-while-genome-assembly
https://bioinformaticsonline.com/pages/view/42275/frequent-parameters-for-bioinformatics-tools