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	<guid isPermaLink="true">https://bioinformaticsonline.com/pages/view/918/data-mining-in-bioinformatics</guid>
	<pubDate>Tue, 16 Jul 2013 03:21:28 -0500</pubDate>
	<link>https://bioinformaticsonline.com/pages/view/918/data-mining-in-bioinformatics</link>
	<title><![CDATA[Data Mining in Bioinformatics]]></title>
	<description><![CDATA[<p>Data mining, the extraction of hidden predictive information from large databases. Data mining is becoming an increasingly important tool to transform this data into information. It is commonly used in a wide range of profiling practices, such as marketing, surveillance, fraud detection and scientific discovery. Data Mining for Bioinformatics enables researchers to meet the challenge of mining vast amounts of biomolecular data to discover real knowledge. In other words, you&rsquo;re a bioinformatician, and data has been dumped in your lap. Find the patterns, trend, answers, or what ever meaningful knowledge the data is hiding. They scour databases for hidden patterns, finding predictive information that experts may miss because it lies outside their expectations.This page Covering theory, algorithms, and methodologies, as well as data mining technologies. Unfortunately life is never simple. In molecular biology, it&rsquo;s becoming more common to generate reams of data then ask someone in bioinformatics to produce an answer. This is exploratory data analysis, one of the most difficult things to do well. Especially if you&rsquo;re thrown in at the deep end.</p><p><strong>Data mining commonly involves four classes of tasks:</strong></p><ul>
<li>Classification - Arranges the data into predefined groups. For example, an email program might attempt to classify an email as legitimate or spam. Common algorithms include decision tree learning, nearest neighbor, naive Bayesian classification and neural networks.</li>
<li>Clustering - Is like classification but the groups are not predefined, so the algorithm will try to group similar items together.</li>
<li>Regression - Attempts to find a function which models the data with the least error.</li>
<li>Association rule learning - Searches for relationships between variables. For example a supermarket might gather data on customer purchasing habits. Using association rule learning, the supermarket can determine which products are frequently bought together and use this information for marketing purposes. This is sometimes referred to as market basket analysis.</li>
<li>From experience, I can say that is one of the most frustrating positions to be in. Data mining is a huge field and can easily be bewildering for a beginner. However, high through-put techniques in molecular biology require, more and more, that bioinformatics is required to interpret the data. Furthermore, people working in bioinformatics generally come from computer science, or biology backgrounds. Data mining, however, involves statistics to one degree or another, which means entering a field that is may not be your strong point.</li>
<li>Excel is fine for creating graphs. If you&rsquo;re serious about data mining though, you&rsquo;ll need something more heavy weight. I use R, free, and with good data mining packages such as vegan and labdsv. For beginners R can be impenetrable, I recommend this book an introduction to R as well as the underlying statistics.</li>
<li>Any of us can rush head on into a land of support vector machines, hidden markov models and neural networks. But coming back to the first point, what are you trying to prove? Always question what are you doing, how does it fit in to the wider picture? Try to regularly review, and keep track of where you are going? This will prevent you from falling into data mining despair.</li>
</ul><p><strong>Data Mining Resources on the net:</strong><br /><br />A laboratory of data mining and bioinformatics is headed by Prof. Ambuj Singh. There are currently seven graduate students in the research group. Our research focuses on image informatics and scalable querying and mining of graphs.For more detail visit:&nbsp;<a href="http://www.cs.ucsb.edu/~dbl/">http://www.cs.ucsb.edu/~dbl/</a></p><p>Here are the materials (Lecture notes) from several past courses on data mining and/or Web mining by Stanford: For detail visit:&nbsp;<a href="http://infolab.stanford.edu/~ullman/mining/mining.html">http://infolab.stanford.edu/~ullman/mining/mining.html</a><br />Statistical Data Mining Tutorial Slides by Andrew Moore The following links point to a set of tutorials on many aspects of statistical data mining, including the foundations of probability, the foundations of statistical data analysis, and most of the classic machine learning and data mining algorithms. For detail visit:&nbsp;<a href="http://www.autonlab.org/tutorials/">http://www.autonlab.org/tutorials/</a></p><p>A tutorial on Introduction to Data Mining for Discovering hidden value in your data warehouse:<a href="http://www.thearling.com/text/dmwhite/dmwhite.htm">http://www.thearling.com/text/dmwhite/dmwhite.htm</a>&nbsp;<br />Wiki Links:&nbsp;<a href="http://en.wikipedia.org/wiki/Data_mining">http://en.wikipedia.org/wiki/Data_mining</a><br />Bioinformatics with Clementine&nbsp;<a href="http://www.spss.ch/upload/1051192224_inseratClemBio.pdf">http://www.spss.ch/upload/1051192224_inseratClemBio.pdf</a>&nbsp;<br />Causal Data Mining in Bioinformatics by Ioannis Tsamardinos:&nbsp;<a href="http://www.forth.gr/ics/bmi/In_the_News/2007/EN69-4.pdf">http://www.forth.gr/ics/bmi/In_the_News/2007/EN69-4.pdf</a></p><p>Report on ACM Text Mining in Bioinformatics (TMBIO 006)&nbsp;<a href="http://www.sigir.org/forum/2007J/2007j_sigirforum_song.pdf">http://www.sigir.org/forum/2007J/2007j_sigirforum_song.pdf</a>&nbsp;<br />BIOKDD 2002: Recent Advances in Data Mining for&nbsp;<br />Bioinformatics:&nbsp;<a href="http://www.acm.org/sigs/sigkdd/explorations/issue4-2/zaki.pdf">http://www.acm.org/sigs/sigkdd/explorations/issue4-2/zaki.pdf</a></p><p><strong>Bioinformatics and Medical Informatics:</strong>&nbsp;<br /><br />Tools for Mining and Applying Genetic Information in Patient Care:<a href="http://www.biomedtechalliance.org/pdfs/03_03_05/03_03_05.pdf">http://www.biomedtechalliance.org/pdfs/03_03_05/03_03_05.pdf</a></p><p>DATA MINING OF MICROARRAY DATABASES FOR HUMAN LUNG CANCER:&nbsp;<a href="http://citeseerx.ist.psu.edu/viewdoc/download?doi=10.1.1.106.385&amp;rep=rep1&amp;type=pdf">http://citeseerx.ist.psu.edu/viewdoc/download?doi=10.1.1.106.385&amp;rep=rep1&amp;type=pdf</a></p><p>Towards knowledge-based gene expression data mining:&nbsp;<a href="http://www.ailab.si/blaz/papers/2007-JBI-BellazziZupan.pdf">http://www.ailab.si/blaz/papers/2007-JBI-BellazziZupan.pdf</a></p><p>DRAFT Accepted for publication in 'Data Mining in Bioinformatics'<br />Jason Wang, Mohammed Zaki, Hannu Toivonen, and Dennis Shasha (Eds.), Springer:<a href="http://www.cs.helsinki.fi/u/htoivone/pubs/gene_mapping_by_pattern_discovery.pdf">http://www.cs.helsinki.fi/u/htoivone/pubs/gene_mapping_by_pattern_discovery.pdf</a></p><p>Data Mining and Text Mining for Bioinformatics: Proceedings of the European Workshop:&nbsp;<a href="http://www.rok.informatik.hu-berlin.de/wbi/research/publications/2003/proceedings_ws_mining.pdf">http://www.rok.informatik.hu-berlin.de/wbi/research/publications/2003/proceedings_ws_mining.pdf</a></p><p><strong>Biological Network Analysis:<br /></strong><br />Graph Mining in Bioinformatics:&nbsp;<a href="http://agbs.kyb.tuebingen.mpg.de/wikis/bg/BNA-5.pdf">http://agbs.kyb.tuebingen.mpg.de/wikis/bg/BNA-5.pdf</a>.</p><p>Text mining in bioinformatics:&nbsp;<a href="http://agbs.kyb.tuebingen.mpg.de/wikis/bg/4.pdf">http://agbs.kyb.tuebingen.mpg.de/wikis/bg/4.pdf</a></p><p>Some datamining books that are available on google books:</p><p>Data mining and bioinformatics: first international workshop, VDMB 2006 By Mehmet M. Dalkilic</p><p>Data mining: concepts and techniques By Jiawei Han, Micheline Kamber</p>]]></description>
	<dc:creator>Jitendra Narayan</dc:creator>
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	<guid isPermaLink="true">https://bioinformaticsonline.com/blog/view/32587/ten-international-scholarships-for-indian-biotechnology-and-bioinformatics-students</guid>
	<pubDate>Wed, 10 May 2017 04:51:02 -0500</pubDate>
	<link>https://bioinformaticsonline.com/blog/view/32587/ten-international-scholarships-for-indian-biotechnology-and-bioinformatics-students</link>
	<title><![CDATA[Ten International Scholarships for Indian Biotechnology and Bioinformatics Students]]></title>
	<description><![CDATA[<p>Wherever you go around the world, Indian students are in demand. With countries such as Canada and Australia providing huge incentives to Indian students to lure them to their shores, there are many institutions around the world that offer scholarships exclusively to Indian students. Historically these scholarships tend to be aimed towards Masters and PhD programmes however on the rise are scholarships for undergraduate students. Student World Online takes a look at ten international scholarships for Indian undergraduate students abroad.</p><p><br /><span><strong>1.&nbsp;</strong></span><strong><a href="http://admissions.cornell.edu/apply/international-students/tata-scholarship"><span>TATA SCHOLARSHIP</span></a></strong>&nbsp;- Cornell University, New York State, USA<br />Tata, the Indian multinational conglomerate company, have a foundation known as the Tata Education &amp; Development Trust which has&nbsp;<span style="text-decoration: underline;"><a href="http://www.news.cornell.edu/stories/2008/10/tata-trust-gives-50-million-endowment-cornell" target="_blank">endowed a multi million dollar sum to Cornell University</a></span>&nbsp;to provide undergraduate scholarships to 20 Indian students every year. &nbsp;In another example of supporting American universities, the Tata group also pledged US$50 million to Harvard University in recent years, whose executive management programme&nbsp;<span style="text-decoration: underline;"><a href="http://en.wikipedia.org/wiki/Ratan_Tata" target="_blank">Ratan Tata</a></span>&nbsp;attended in the 1970s. &nbsp;<a href="http://admissions.cornell.edu/apply/international-students/tata-scholarship" target="_blank"><span><span style="text-decoration: underline;">Read more...&nbsp;</span></span></a>&nbsp;<br /><br /><strong><span>2.</span></strong>&nbsp;<a href="http://www.uow.edu.au/future/international/apply/scholarships/UOW135799.html" target="_blank"><strong><span>BRADMAN FOUNDATION SCHOLARSHIP</span></strong></a>&nbsp;- University of Wollongong, Australia.<br />Named after Australia's cricket legend&nbsp;<span style="text-decoration: underline;"><a href="http://en.wikipedia.org/wiki/Donald_Bradman" target="_blank">Donald Bradman</a></span>, the&nbsp;<span style="text-decoration: underline;"><a href="https://www.uow.edu.au/content/groups/public/@web/@unia/documents/doc/uow145334.pdf" target="_blank">UOW Bradman Foundation Scholarship</a></span>&nbsp;was launched in 2012, with the help of Adam Gilchrist no less, to offer one successful Indian student each year a 50% reduction in tuition fees. &nbsp;<a href="http://www.uow.edu.au/future/international/apply/scholarships/UOW135799.html" target="_blank"><span><span style="text-decoration: underline;">Read more...</span></span></a>&nbsp;&nbsp;</p><p><span><strong>3.&nbsp;</strong></span><strong><a href="http://www.huaweischolarships.org/about_scholar.aspx" target="_blank"><span>HUAWEI MAITREE SCHOLARSHIPS</span></a></strong>&nbsp;- Various Universities, China<br />Along with Tata, Huawei are the other huge corporation to be featured. &nbsp;China's massive telecoms equipment vendor are involved in these scholarships offered to Indian students studying in China. &nbsp;In 2013 there are 10 generous scholarships available which provide full tuition fees and living expenses. &nbsp;The courses on which the scholarships are offered include Science and Technology courses, Social Sciences and Culture and Development courses. &nbsp;<a href="http://www.huaweischolarships.org/about_scholar.aspx" target="_blank"><span><span style="text-decoration: underline;">Read more...</span></span></a></p><p><span><strong>4.&nbsp;</strong></span><strong><a href="http://www.britishcouncil.in/study-uk/dr-manmohan-singh-scholarships-2013" target="_blank"><span>DR. MANMOHAN SINGH SCHOLARSHIPS</span></a></strong>&nbsp;- Cambridge University, England, UK<br />These scholarships have been designed to help budding Indian minds follow in the footsteps of&nbsp;<span style="text-decoration: underline;"><a href="http://pmindia.nic.in/" target="_blank">Indian prime minister Manmohan Singh</a></span>&nbsp;by studying at the prestigious Cambridge University. &nbsp;The scholarships can be applied to any undergarduate course (with the two exceptions of medicine and veterinary science) and cover everything, i.e. tuition and college fees, living expenses and an additional grant to go towards travel expenses. &nbsp;<a href="http://www.britishcouncil.in/study-uk/dr-manmohan-singh-scholarships-2013" target="_blank"><span><span style="text-decoration: underline;">Read more...</span></span></a><br /><br /><span><strong>5.&nbsp;</strong></span><strong><a href="http://www.oxbridgeindia.com/scholarship.php"><span>OXFORD AND CAMBRIDGE SOCIETY OF INDIA</span></a></strong>&nbsp;- Oxford &amp; Cambridge Universities, England, UK<br />As the name might suggest, these are scholarships available for students wishing to study at Oxford or Cambridge (cleverly known together as&nbsp;<span style="text-decoration: underline;"><a href="http://en.wikipedia.org/wiki/Oxbridge" target="_blank">Oxbridge</a></span>). &nbsp;It is only available for applicants who are completing or have completed a degree at an Indian university, however these scholarships are for both undergraduate and graduate students.&nbsp;&nbsp;<a href="http://www.oxbridgeindia.com/scholarship.php" target="_blank"><span><span style="text-decoration: underline;">Read more...</span></span></a></p><p><span><strong>6.&nbsp;</strong></span><strong><a href="http://www.napier.ac.uk/study/international/funding/Pages/india-scholarships.aspx" target="_blank"><span>EDINBURGH NAPIER UNIVERSITY</span></a></strong>&nbsp;- Scotland, UK<br />This one applies to all countries in the Indian subcontinent and is for both undergraduate and graduate courses. Edinburgh Napier University offers a merit based discount of &pound;2,000 Pounds. &nbsp;<a href="http://www.napier.ac.uk/study/international/funding/Pages/india-scholarships.aspx" target="_blank"><span><span style="text-decoration: underline;">Read more...</span></span></a></p><p><span><strong>7.&nbsp;</strong></span><strong><a href="http://www.sheffield.ac.uk/international/countries/asia/south-asia/india/scholarships" target="_blank"><span>SHEFFIELD UNIVERSITY</span></a></strong>&nbsp;- Sheffield, UK<br />Provides merit-based scholarships for undergraduate and graduate programmes across all subjects<span>.</span>&nbsp;<a href="http://www.sheffield.ac.uk/international/countries/asia/south-asia/india/scholarships" target="_blank"><span><span style="text-decoration: underline;">Read more...</span></span></a><br /><br /><span><strong>8.&nbsp;</strong></span><strong><a href="http://www.india4eu.eu/scholarships" target="_blank"><span>INDIA 4EU II</span></a></strong>&nbsp;- Several Universities across Europe<br />Pioneered by the European Union and involving partner universities in France, Finland, Germany, Italy, Portugal, Spain and Sweden,&nbsp;<span style="text-decoration: underline;"><a href="http://www.india4eu.eu/" target="_blank">the India 4EU II initiative</a></span>&nbsp;is aimed at encouraging Indian students to study, work and live in Europe. &nbsp;The initiative is well funded and allows the successful students tuition fees, expenses for living and travel costs as well as insurance during their time at one of the partner universities. &nbsp;<a href="http://www.india4eu.eu/scholarships" target="_blank"><span><span style="text-decoration: underline;">Read more...</span></span></a><br /><br /><span><strong>9.&nbsp;</strong></span><strong><a href="http://www.tcd.ie/international/Indian%20Scholarship.php" target="_blank"><span>TRINITY COLLEGE DUBLIN</span></a></strong>&nbsp;- Ireland<br />Valid for undergraduate courses in the faculties of Arts, Humanities, Social Sciences, Science, Computer Science or Engineering, the Trinity College Dublin offers Indian students scholarships to the tune of&nbsp;&euro;9,000 per annum over a year degree course. &nbsp;<a href="http://www.tcd.ie/international/Indian%20Scholarship.php" target="_blank"><span><span style="text-decoration: underline;">Read more...</span></span></a><br /><br /><span><strong>10.&nbsp;</strong></span><strong><a href="http://www.indianexpress.com/news/university-college-dublin-announces--euro-250000-scholarship-for-indian-students/1094390/" target="_blank"><span>UNIVERSITY COLLEGE DUBLIN</span></a></strong>&nbsp;- Ireland<br />Another of Ireland and Dublin's finest, the UCD awards one Global Excellence Undergraduate Scholarship which provides the worthy student a substantial 50% towards their tuition fees and is valid for all courses save medicine, radiography and veterinary medicine. &nbsp;UCD also offers a Global Undergraduate Scholarship scheme for undergrads accepted on science, social sciences, arts and business courses. &nbsp;This is all thanks to a &euro;250,000 fund that will allow for 57 Indian students to benefit from scholarships at UCD. &nbsp;<a href="http://www.indianexpress.com/news/university-college-dublin-announces--euro-250000-scholarship-for-indian-students/1094390/"><span><span style="text-decoration: underline;">Read more...</span></span></a></p>]]></description>
	<dc:creator>Priya Singh</dc:creator>
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	<guid isPermaLink="true">https://bioinformaticsonline.com/news/view/1219/research-with-help-of-bioinformatics-helpful</guid>
	<pubDate>Fri, 02 Aug 2013 11:20:24 -0500</pubDate>
	<link>https://bioinformaticsonline.com/news/view/1219/research-with-help-of-bioinformatics-helpful</link>
	<title><![CDATA[Research with help of bioinformatics helpful]]></title>
	<description><![CDATA[<p>Endocrinologist G.R. Sridhar says</p><blockquote><p>Research with the help of bioinformatics with a trans-disciplinary approach is yielding good results.</p><p>http://www.thehindu.com/features/education/research/research-with-help-of-bioinformatics-helpful/article2295629.ece</p></blockquote>]]></description>
	<dc:creator>Jit</dc:creator>
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	<guid isPermaLink="true">https://bioinformaticsonline.com/blog/view/1295/five-points-for-bioinformatics-softwaretools</guid>
	<pubDate>Mon, 05 Aug 2013 04:12:32 -0500</pubDate>
	<link>https://bioinformaticsonline.com/blog/view/1295/five-points-for-bioinformatics-softwaretools</link>
	<title><![CDATA[Five points for bioinformatics software/tools]]></title>
	<description><![CDATA[<p><span>In the bioinformatics sector we mostly spend time on computational analysis of huge amounts of data and try to make sense of it, biologically. But, most of the newbie bioinformaticians are faced with dilemma when they receive biological sequence data for the first time. They mostly found confusing over open source, user friendly GUI, and commercial bioinformatics software. Don&rsquo;t be surprise this is true and also not an easy task to decide, because analytical step is the most crucial part and believe to be the biggest bottleneck in publishing paper in high impact journals. Through this blog I would like to address the pros and cons of both kind of software/tools and try to assist (Hmmm not really, It looks convince) you to make decision on your software selections.</span></p><p><span><img src="http://bioinformaticsonline.com/mod/photo/five.jpg" alt="image" style="border: 0px;"></span></p><p><span>The most common newbie questions are:</span><span></span></p><p><span>Should I try to use these free open source programs? &nbsp;Why are we not trying GUI software for computational analysis? Should I use commercial bioinformatics programs/software?&rdquo;</span><span><br /></span><span><br />1. Let&rsquo;s be open</span><span></span></p><p><span>We generally think free and cheap are useless. But this concept is not applicable when we discuss open source software. Mostly, the bioinformatics software is developed by highly competitive biological programmers who believe in open sharing of knowledge. They come under Open Bioinformatics Foundation or O|B|F which is a non-profit, volunteer run organization focused on supporting open source programming in bioinformatics. The best part about open source tools/software is that they&rsquo;re free to download the source code and read exactly what the program does. If you are so inclined, you can view all of the parts of the program and see the logical flow of the pipeline. In addition, open source makes an excellent learning tool for any beginning bioinformatician. Moreover, you can modify existing open source programs to deal with cutting-edge problems or to customize your pipeline.</span><span>&nbsp;</span><span>Apart from your computational and analysis work, most of the reviewer also prefers the open source based results so that they can validate the results if validation required.</span></p><p><span>2. Code headache</span><span></span></p><p><span>As a bioinformatician you are supposed to know the basics of programming languages, and if you are not good at it, then please learn it as soon as possible because you are not a bio-analyst but biological programmers. The<span>&nbsp;</span>open source programs usually lack dedicated service and support teams (often because they were the product of an overworked doc/postdoc!) so you are responsible for troubleshooting your own errors most of the time.<span>&nbsp;</span>We commonly receive the HELP email to support and assist to setup the pipeline; you can also find this kind of request on any QA forum. I personally believe this coding horror brings the biggest downside of open-source programs; where you need some programming skills in order to implement the program in your pipeline. But, if you are not able to fix the pipeline and modify the open source code according to your requirements them you should re-think on your bioinformatician name tag!!!</span><span></span></p><p><span>3. Dive into the codes</span><span></span></p><p><span>Some of the biologist turn bioinformatician says &ldquo;if you can do the same thing with commercial software then why to get migraine with weird codes&rdquo;, well this statement looks to me that guys are keen to learn swimming but still don&rsquo;t like to get wet. If you are still using paid software and doing your work by customer support and clicking some of the well-designed GUI button then perhaps you are not interested in learning and trying new and challenging bioinformatics works. You are missing the basic flavour of bioinformatics. Let&rsquo;s dive into the coding world, I am sure your will enjoy it. I recommend your to swim freely in code&rsquo;s sea, and enjoy the journey; do not merely watch it from the outside. &nbsp;</span></p><p><span>4. Paid does not mean better</span><span></span></p><p><span>The bioinformatics company which are specializes in bioinformatics solutions develop well designed/packed, user friendly software by using a large number of specialised scientist, programmers and support staff. They also provide good services to accomplice your biological analysis work. This means that if you hit a &lsquo;snag&rsquo; with your data, help is likely only a phone call away! These companies price their products competitively against the cost of a dedicated bioinformatician. You may be able to afford the program, but not the additional staff! Additionally, most of the functionality that you need in your analysis is already coded into the program. Need to plot a graph? Just click this button right here. It is that easy.</span><span>&nbsp;</span><span>But, as a bioinformatician this is not generally well encouraged approach in biological analysis work, because the software is not available to everyone and your data can&rsquo;t be validated. Moreover, there is very less chances that anyone will repeat your work or love to do similar kind of research (because not all the labs in the world are rich like yours).</span></p><p><span>5. Take a caution<br /><br />In biological analysis work, in which you deal GB/TB of data are having maximum chances of getting errors, so please be careful and always cross check your data before coming to any conclusion. Even an error in two line code can alter your entire analysis and display weird results. Some of the scientist blindly believes on commercial software, which is entirely wrong. Using proprietary tools does not absolve you of the need to actually read and research the type of analysis that you are doing. This is particularly true in the case of genome assembly and annotation.</span></p><p><span><br />At the end, I would like to tell only one think that open source solutions allows you to do more cutting edge analysis than the commercial tools. So let&rsquo;s go for it.</span></p><p>Disclaimer:</p><p>This is my personal view. I have nothing to do with any company or open source community.&nbsp;The views expressed on these pages are mine alone and not those of my current/past employers. I do reserve the right to remove comments left by spammers or off-topic comments.</p>]]></description>
	<dc:creator>Jitendra Narayan</dc:creator>
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	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/32726/ergo-20-bioinformatics-suites</guid>
	<pubDate>Tue, 16 May 2017 08:14:10 -0500</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/32726/ergo-20-bioinformatics-suites</link>
	<title><![CDATA[ERGO 2.0 Bioinformatics suites]]></title>
	<description><![CDATA[<p>ERGO 2.0 provides a systems biology informatics toolkit centered on comparative genomics to capture, query, and visualize sequenced genomes. &nbsp;Using Igenbio's proprietary algorithms, and the most comprehensive genomic database integrated with the largest collection of microbial metabolic and non-metabolic pathways, ERGO&trade; assigns functions to genes, integrates genes into pathways, and identifies previously unknown or mischaracterized genes, cryptic pathways, and gene products.&nbsp;</p><p>Address of the bookmark: <a href="https://www.igenbio.com/ergo/" rel="nofollow">https://www.igenbio.com/ergo/</a></p>]]></description>
	<dc:creator>Jit</dc:creator>
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	<guid isPermaLink="true">https://bioinformaticsonline.com/pages/view/34814/bioinformatics-web-application-development-with-perl</guid>
	<pubDate>Tue, 26 Dec 2017 18:14:11 -0600</pubDate>
	<link>https://bioinformaticsonline.com/pages/view/34814/bioinformatics-web-application-development-with-perl</link>
	<title><![CDATA[Bioinformatics Web Application Development with Perl]]></title>
	<description><![CDATA[<div><p>Perl's second wave of adoption came from the growth of the world wide web. Dynamic web pages&mdash;the precursor to modern web applications&mdash;were easy to create with Perl and CGI. Thanks to Perl's ubiquity as a language for system administrators and its power to manipulate text, it was the default choice for web programming. Its presence everywhere made it popular and, in some ways, the duct tape of the Internet.</p><h4>Web Application Development</h4><p>The old days of CGI programs and the simple development style that represented seem clunky. Web pages have become web applications. Development has moved from generating static HTML to both client and server side programming, with rich client interfaces and powerful backends.</p><p>Perl is still well suited for developing modern web apps. The language grows more powerful and easier to use every year, the available libraries are wonderful and keep getting better, and the inventions and discoveries available in modern Perl are unsurpassed.</p><p>In particular, a modern Perl developer can do amazing things with modern Perl tools. If you still think of Perl web development as a&nbsp;<em>cgi-bin</em>&nbsp;directory full of messy scripts that spew warnings to STDERR, you're a decade out of date. Better yet, you can replace that mess piecemeal, thanks to the new tools and techniques of modern Perl. See, for example, the ever-growing list of technologies&nbsp;<a href="http://www.builtinperl.com/">Built in Perl</a>.</p><h4>Modern Perl Web Frameworks</h4><p>While the old wave of web development may have made the CGI.pm module central, modern Perl web programming follows a stricter separation of business logic, URL and request routing, and output. The days of slinging a string here, an array there, a Perl hash yonder, declaring every variable at the top of the program, and maybe making a subroutine are gone. The Perl world has seen the value of abstraction and ways to mechanize away boilerplate. Perl has dozens of frameworks and toolkits designed to make web development and deployment simpler.</p><p>Any of a dozen of these frameworks will help you do great things, but three in particular stand out. You can build web sites and web applications of tremendous value with all three. These are neither the only good possibilities (think of POE or Jifty or Continuity or...) nor the only mechanisms for web programming with Perl (see Mechanize or LWP or Mojo::UserAgent for more). Yet if you want three good options to choose between, start here.</p><h4>Catalyst</h4><p>The&nbsp;<a href="http://catalystframework.org/">Catalyst</a>&nbsp;framework is a flexible and powerful system for building small to large web apps. It uses the&nbsp;<a href="http://moose.perl.org/">Moose</a>&nbsp;object system to provide great APIs for extension and further development. It's the most mature of the modern top Perl web frameworks, yet it retains its flexibility and vibrancy. In particular, its plugin and extension ecosystem allows it to evolve to provide new and essential features.</p><p>Catalyst has embraced the Plack/PSGI standard for Perl web deployment and recent versions are exploring high-scalability, event-based request handling models.</p><h4>Dancer</h4><p>The&nbsp;<a href="http://perldancer.org/">Dancer</a>&nbsp;framework is deliberately minimal in syntax and scope, but it also has a vibrant plugin ecosystem. Dancer particularly excels for smaller sites and applications, though good programmers can build larger things with it.</p><p>The first version of Dancer was easy to use. Dancer 2 continues that ease while improving the internals and robustness of applications.</p><h4>Mojolicious</h4><p>The&nbsp;<a href="http://mojolicio.us/">Mojolicious</a>&nbsp;(Mojo) framework has a real-time design based on high performance event handling. Its focus is solving new and interesting problems in simple and effective ways, and the project has produced a lot of new code that does old things in better ways.</p><p>In particular, Mojolicious goes to great lengths to support new web standards, such as CSS 3, web sockets, and HTTP 2.</p><p>Where Catalyst embraces the CPAN fully, Mojolicious by design provides most of what an average app might need in a single download. It's still fully compatible with the CPAN, but the intention is to provide good working defaults in a package that's easy to start with. Mojo's fans are quick to praise it as fun to develop.</p><p>A modern Perl web developer should be familiar with at least one of these frameworks.</p><h4>Modern Perl Storage Mechanisms</h4><p>Perl's venerable&nbsp;<a href="http://search.cpan.org/perldoc?DBI">DBI</a>&nbsp;module has been the focal point of database access since its invention. Its design allows it to provide the same interface to huge relational databases and flat files alike through its DBD extension mechanism. Yet the DBI by itself isn't the be-all, end-all of data storage and access in Perl.</p><h4>DBIx::Class</h4><p><a href="http://search.cpan.org/perldoc?DBIx::Class">DBIx::Class</a>&nbsp;sits on top of DBI to provide an API to your database based on the concept of queries and results. This is often sufficient to remove all but the most complicated of SQL from your code, leaving you to manipulate your business models instead of the small details of how a relational database works. The power and maintainability you receive is well the small cost of the learning curve.</p><p>Even better, DBIC can manage (and even generate) your database schema for you.</p><p>Recent versions of DBIC have demonstrated that a well-written ORM can perform much better than even clever hand-written code. Because it builds on the Perl DBI, it scales everywhere from SQLite to PostgreSQL, MySQL, Oracle, and more.</p><h3>Rose::DB</h3><p>The lesser-known but no less powerful&nbsp;<a href="http://search.cpan.org/perldoc?Rose::DB::Object">Rose::DB::Object</a>&nbsp;builds on&nbsp;<a href="http://search.cpan.org/perldoc?Rose::DB">Rose::DB</a>&nbsp;to provide an object-relational mapper for Perl. While its high level features most directly compare to those of DBIx::Class, it's often measurably faster.</p><h4>NoSQL on the CPAN</h4><p>Of course the&nbsp;<a href="http://search.cpan.org/">CPAN</a>&nbsp;has modules for almost any NoSQL database or job queue or persistence mechanism you could name, and several you have never heard of. Everything you need is a quick CPAN or cpanm away!</p><h4>Modern Perl Deployment Strategies</h4><p>In the early days of the web, deploying a Perl web application meant putting one or more&nbsp;<em>.cgi</em>&nbsp;or&nbsp;<em>.pl</em>&nbsp;files in a special directory and hoping that your system administrator had everything configured correctly. The execution model was often slow and cumbersome, and accessing shared resources such as databases was often tricky.</p><p>Modern Perl has better choices. While deployment strategies are the source of many arguments, the return on your investment from learning the modern way is impressive.</p><h4>Plack/PSGI</h4><p>The PSGI specification (as exemplified by&nbsp;<a href="http://plackperl.org/">Plack</a>) describes a strategy for building Perl web apps independent of server and with the possibility to share custom processing behaviors.</p><p>In other words, it's a standard for writing Perl apps to take advantage of the huge ecosystem of Perl development available on the CPAN without tying yourself to a server like Apache, Apache 2, nginx, or anything else.</p><p>Any good modern Perl web framework (including those listed here) supports PSGI. Several deployment mechanisms exist to meet various business needs which also support PSGI. In particular, you can deploy the same application with a local testing server on your own machine as you can to your production server or servers without changing your application at all.</p><h4>mod_perl</h4><p>The older but still viable mod_perl Apache httpd module embeds Perl into the web server. This was the first widespread persistence mechanism for Perl web applications themselves and it's still popular to this day, though PSGI compliance is often the choice for new development. (PSGI handlers to use mod_perl as the backend are available.)</p><p>Modern Perl developers should familiarize themselves with PSGI and the wealth of available Plack middleware.</p><h4>Perl Web Development</h4><p>Of course no discussion of Perl web development would be complete without mentioning the strength of the CPAN. Almost any project will benefit from the wealth of freely available libraries built to solve real problems. These distributions run the gamut from full-blown web frameworks and content management systems to APIs for web services, development tools, testing systems, and interfaces to document formats and external resources.</p><p>For example, if you need to write a web service which accepts JSON data and produces Excel spreadsheets, you can glue together a few CPAN distributions and get the job done early. If you need to consume XML from a remote service and emit a PDF, you're in luck.</p><p>Perl's prowess as a general purpose programming language as well as its flexibility and power in managing text and gluing systems together make it a wonderful fit for web development. The community's adoption of modern Perl standards such as PSGI and Plack only enhance your power.</p><p>Web application development in Perl is still viable, and modern Perl tools and techniques and libraries make it more powerful and pleasant than ever.</p></div>]]></description>
	<dc:creator>Jit</dc:creator>
</item>
<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/view/2021</guid>
	<pubDate>Mon, 12 Aug 2013 09:27:57 -0500</pubDate>
	<link>https://bioinformaticsonline.com/view/2021</link>
	<title><![CDATA[What are the difference between BioRuby and BioGem?]]></title>
	<description><![CDATA[<p>I came across two diferent but matching term BioRuby and BioGem. What are the difference between these two term? If both are using same Ruby language for development then why did they develope two different biological packages.</p>]]></description>
	<dc:creator>Neel</dc:creator>
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<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/pages/view/35525/linux-commands-cheat-sheet-for-bioinformatics-and-computational-biology-professionals</guid>
	<pubDate>Mon, 05 Feb 2018 18:50:41 -0600</pubDate>
	<link>https://bioinformaticsonline.com/pages/view/35525/linux-commands-cheat-sheet-for-bioinformatics-and-computational-biology-professionals</link>
	<title><![CDATA[Linux Commands Cheat Sheet for Bioinformatics and Computational Biology Professionals]]></title>
	<description><![CDATA[<p><span>The purpose of this cheat sheet is to introduce biologist and bioinformatician to the frequently used tools for NGS analysis as well as giving experience in writing one-liners.</span></p><ul>
<li><span></span><span><strong>File System</strong></span><span><strong><br /> </strong></span><span>ls</span><span>&nbsp;&mdash; list items in current directory</span><span><br /> </span><span>ls -l</span><span>&nbsp;&mdash; list items in current directory and show in long format to see perimissions, size, and modification date</span><span><br /> </span><span>ls -a</span><span>&nbsp;&mdash; list all items in current directory, including hidden files</span><span><br /> </span><span>ls -F</span><span>&nbsp;&mdash; list all items in current directory and show directories with a slash and executables with a star</span><span><br /> </span><span>ls dir</span><span>&nbsp;&mdash; list all items in directory dir</span><span><br /> </span><span>cd dir</span><span>&nbsp;&mdash; change directory to dir</span><span><br /> </span><span>cd ..</span><span>&nbsp;&mdash; go up one directory</span><span><br /> </span><span>cd /</span><span>&nbsp;&mdash; go to the root directory</span><span><br /> </span><span>cd ~</span><span>&nbsp;&mdash; go to to your home directory</span><span><br /> </span><span>cd -</span><span>&nbsp;&mdash; go to the last directory you were just in</span><span><br /> </span><span>pwd</span><span>&nbsp;&mdash; show present working directory</span><span><br /> </span><span>mkdir dir</span><span>&nbsp;&mdash; make directory dir</span><span><br /> </span><span>rm file</span><span>&nbsp;&mdash; remove file</span><span><br /> </span><span>rm -r dir</span><span>&nbsp;&mdash; remove directory dir recursively</span><span><br /> </span><span>cp file1 file2</span><span>&nbsp;&mdash; copy file1 to file2</span><span><br /> </span><span>cp -r dir1 dir2</span><span>&nbsp;&mdash; copy directory dir1 to dir2 recursively</span><span><br /> </span><span>mv file1 file2</span><span>&nbsp;&mdash; move (rename) file1 to file2</span><span><br /> </span><span>ln -s file link</span><span>&nbsp;&mdash; create symbolic link to file</span><span><br /> </span><span>touch file</span><span>&nbsp;&mdash; create or update file</span><span><br /> </span><span>cat file</span><span>&nbsp;&mdash; output the contents of file</span><span><br /> </span><span>less file</span><span>&nbsp;&mdash; view file with page navigation</span><span><br /> </span><span>head file</span><span>&nbsp;&mdash; output the first 10 lines of file</span><span><br /> </span><span>tail file</span><span>&nbsp;&mdash; output the last 10 lines of file</span><span><br /> </span><span>tail -f file</span><span>&nbsp;&mdash; output the contents of file as it grows, starting with the last 10 lines</span><span><br /> </span><span>vim file</span><span>&nbsp;&mdash; edit file</span><span><br /> </span><span>alias name 'command'</span><span>&nbsp;&mdash; create an alias for a command</span><span><br /> </span></li>
<li><span></span><span><strong>System</strong></span><span><strong><br /> </strong></span><span>shutdown</span><span>&nbsp;&mdash; shut down machine</span><span><br /> </span><span>reboot</span><span>&nbsp;&mdash; restart machine</span><span><br /> </span><span>date</span><span>&nbsp;&mdash; show the current date and time</span><span><br /> </span><span>whoami</span><span>&nbsp;&mdash; who you are logged in as</span><span><br /> </span><span>finger user</span><span>&nbsp;&mdash; display information about user</span><span><br /> </span><span>man command</span><span>&nbsp;&mdash; show the manual for command</span><span><br /> </span><span>df</span><span>&nbsp;&mdash; show disk usage</span><span><br /> </span><span>du</span><span>&nbsp;&mdash; show directory space usage</span><span><br /> </span><span>free</span><span>&nbsp;&mdash; show memory and swap usage</span><span><br /> </span><span>whereis app</span><span>&nbsp;&mdash; show possible locations of app</span><span><br /> </span><span>which app</span><span>&nbsp;&mdash; show which app will be run by default</span><span><br /> </span></li>
<li><span></span><span><strong>Process Management</strong></span><span><strong><br /> </strong></span><span>ps</span><span>&nbsp;&mdash; display your currently active processes</span><span><br /> </span><span>top</span><span>&nbsp;&mdash; display all running processes</span><span><br /> </span><span>kill pid</span><span>&nbsp;&mdash; kill process id pid</span><span><br /> </span><span>kill -9 pid</span><span>&nbsp;&mdash; force kill process id pid</span><span><br /> </span></li>
<li><span></span><span><strong>Permissions</strong></span><span><strong><br /> </strong></span><span>ls -l</span><span>&nbsp;&mdash; list items in current directory and show permissions</span><span><br /> </span><span>chmod ugo file</span><span>&nbsp;&mdash; change permissions of file to ugo - u is the user's permissions, g is the group's permissions, and o is everyone else's permissions. The values of u, g, and o can be any number between 0 and 7.</span><span><br /> </span><span>7</span><span>&nbsp;&mdash; full permissions</span><span><br /> </span><span>6</span><span>&nbsp;&mdash; read and write only</span><span><br /> </span><span>5</span><span>&nbsp;&mdash; read and execute only</span><span><br /> </span><span>4</span><span>&nbsp;&mdash; read only</span><span><br /> </span><span>3</span><span>&nbsp;&mdash; write and execute only</span><span><br /> </span><span>2</span><span>&nbsp;&mdash; write only</span><span><br /> </span><span>1</span><span>&nbsp;&mdash; execute only</span><span><br /> </span><span>0</span><span>&nbsp;&mdash; no permissions</span><span><br /> </span><span>chmod 600 file</span><span>&nbsp;&mdash; you can read and write - good for files</span><span><br /> </span><span>chmod 700 file</span><span>&nbsp;&mdash; you can read, write, and execute - good for scripts</span><span><br /> </span><span>chmod 644 file</span><span>&nbsp;&mdash; you can read and write, and everyone else can only read - good for web pages</span><span><br /> </span><span>chmod 755 file</span><span>&nbsp;&mdash; you can read, write, and execute, and everyone else can read and execute - good for programs that you want to share</span><span><br /> </span></li>
<li><span></span><span><strong>Networking</strong></span><span><strong><br /> </strong></span><span>wget file</span><span>&nbsp;&mdash; download a file</span><span><br /> </span><span>curl file</span><span>&nbsp;&mdash; download a file</span><span><br /> </span><span>scp user@host:file dir</span><span>&nbsp;&mdash; secure copy a file from remote server to the dir directory on your machine</span><span><br /> </span><span>scp file user@host:dir</span><span>&nbsp;&mdash; secure copy a file from your machine to the dir directory on a remote server</span><span><br /> </span><span>scp -r user@host:dir dir</span><span>&nbsp;&mdash; secure copy the directory dir from remote server to the directory dir on your machine</span><span><br /> </span><span>ssh user@host</span><span>&nbsp;&mdash; connect to host as user</span><span><br /> </span><span>ssh -p port user@host</span><span>&nbsp;&mdash; connect to host on port as user</span><span><br /> </span><span>ssh-copy-id user@host</span><span>&nbsp;&mdash; add your key to host for user to enable a keyed or passwordless login</span><span><br /> </span><span>ping host</span><span>&nbsp;&mdash; ping host and output results</span><span><br /> </span><span>whois domain</span><span>&nbsp;&mdash; get information for domain</span><span><br /> </span><span>dig domain</span><span>&nbsp;&mdash; get DNS information for domain</span><span><br /> </span><span>dig -x host</span><span>&nbsp;&mdash; reverse lookup host</span><span><br /> </span><span>lsof -i tcp:1337</span><span>&nbsp;&mdash; list all processes running on port 1337</span><span><br /> </span></li>
<li><span></span><span><strong>Searching</strong></span><span><strong><br /> </strong></span><span>grep pattern files</span><span>&nbsp;&mdash; search for pattern in files</span><span><br /> </span><span>grep -r pattern dir</span><span>&nbsp;&mdash; search recursively for pattern in dir</span><span><br /> </span><span>grep -rn pattern dir</span><span>&nbsp;&mdash; search recursively for pattern in dir and show the line number found</span><span><br /> </span><span>grep -r pattern dir --include='*.ext</span><span>&nbsp;&mdash; search recursively for pattern in dir and only search in files with .ext extension</span><span><br /> </span><span>command | grep pattern</span><span>&nbsp;&mdash; search for pattern in the output of command</span><span><br /> </span><span>find file</span><span>&nbsp;&mdash; find all instances of file in real system</span><span><br /> </span><span>locate file</span><span>&nbsp;&mdash; find all instances of file using indexed database built from the updatedb command. Much faster than find</span><span><br /> </span><span>sed -i 's/day/night/g' file</span><span>&nbsp;&mdash; find all occurrences of day in a file and replace them with night - s means substitude and g means global - sed also supports regular expressions</span><span><br /> </span></li>
<li><span></span><span><strong>Compression</strong></span><span><strong><br /> </strong></span><span>tar cf file.tar files</span><span>&nbsp;&mdash; create a tar named file.tar containing files</span><span><br /> </span><span>tar xf file.tar</span><span>&nbsp;&mdash; extract the files from file.tar</span><span><br /> </span><span>tar czf file.tar.gz files</span><span>&nbsp;&mdash; create a tar with Gzip compression</span><span><br /> </span><span>tar xzf file.tar.gz</span><span>&nbsp;&mdash; extract a tar using Gzip</span><span><br /> </span><span>gzip file</span><span>&nbsp;&mdash; compresses file and renames it to file.gz</span><span><br /> </span><span>gzip -d file.gz</span><span>&nbsp;&mdash; decompresses file.gz back to file</span><span><br /> </span></li>
<li><span></span><span><strong>Shortcuts</strong></span><span><strong><br /> </strong></span><span>ctrl+a</span><span>&nbsp;&mdash; move cursor to beginning of line</span><span><br /> </span><span>ctrl+f</span><span>&nbsp;&mdash; move cursor to end of line</span><span><br /> </span><span>alt+f</span><span>&nbsp;&mdash; move cursor forward 1 word</span><span><br /> </span><span>alt+b</span><span>&nbsp;&mdash; move cursor backward 1 word</span><span><br /> </span></li>
<li></li>
</ul>]]></description>
	<dc:creator>Rahul Nayak</dc:creator>
</item>
<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/videolist/watch/4090/computational-biology-in-the-21st-century-making-sense-out-of-massive-data</guid>
	<pubDate>Thu, 29 Aug 2013 08:32:26 -0500</pubDate>
	<link>https://bioinformaticsonline.com/videolist/watch/4090/computational-biology-in-the-21st-century-making-sense-out-of-massive-data</link>
	<title><![CDATA[Computational Biology in the 21st Century: Making Sense out of Massive Data]]></title>
	<description><![CDATA[<iframe width="" height="" src="https://www.youtube-nocookie.com/embed/I99UiA_vaJQ" frameborder="0" allowfullscreen></iframe>Computational Biology in the 21st Century: Making Sense out of Massive Data    
    
Air date:  Wednesday, February 01, 2012, 3:00:00 PM
Category:  Wednesday Afternoon Lectures  
 
Description:  The last two decades have seen an exponential increase in genomic and biomedical data, which will soon outstrip advances in computing power to perform current methods of analysis. Extracting new science from these massive datasets will require not only faster computers; it will require smarter algorithms. We show how ideas from cutting-edge algorithms, including spectral graph theory and modern data structures, can be used to attack challenges in sequencing, medical genomics and biological networks. 

The NIH Wednesday Afternoon Lecture Series includes weekly scientific talks by some of the top researchers in the biomedical sciences worldwide. 

Author:  Dr. Bonnie Berger  
Runtime:  00:58:06  
Permanent link:  http://videocast.nih.gov/launch.asp?17563]]></description>
	
</item>
<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/pages/view/35983/some-useful-bioinformatics-links</guid>
	<pubDate>Fri, 16 Mar 2018 20:50:10 -0500</pubDate>
	<link>https://bioinformaticsonline.com/pages/view/35983/some-useful-bioinformatics-links</link>
	<title><![CDATA[Some useful Bioinformatics links]]></title>
	<description><![CDATA[<p><br /> Reference-free prediction of rearrangement breakpoint reads | Bioinformatics | Oxford Academic</p><p>https://academic.oup.com/bioinformatics/article/30/18/2559/2475628<br /> Reference-free SNP detection: dealing with the data deluge</p><p>https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4083407/<br /> GATB/DiscoSnp: DiscoSnp is designed for discovering all kinds of SNPs (not only isolated ones), as well as insertions and deletions, from raw set(s) of reads.</p><p>https://github.com/GATB/DiscoSnp<br /> De novo assembly | Oxford Nanopore Technologies</p><p>https://nanoporetech.com/taxonomy/term/131<br /> De novo long-read assembly of a complex animal genome | bioRxiv</p><p>https://www.biorxiv.org/content/early/2017/09/10/187054<br /> Rapid de novo assembly of the European eel genome from nanopore sequencing reads | Scientific Reports</p><p>https://www.nature.com/articles/s41598-017-07650-6.epdf?author_access_token=dktG7e98wyRJnaEEMTcPqtRgN0jAjWel9jnR3ZoTv0P7E7t-wVGo30iojNO7dICajNY_7PE5xVPv6OoLe7hn9TeUjcZ5umREOzNoPMWkfYH58RS6uxm3vm4e4BG2AA_WKW84i6egKK271NwMq-NfzA%3D%3D<br /> nanoporetech/ont-assembly-polish: ONT assembly and Illumina polishing pipeline</p><p>https://github.com/nanoporetech/ont-assembly-polish<br /> Generade-nl/TULIP: TULIP - The Uncorrected Long read Itegration Pipeline</p><p>https://github.com/Generade-nl/TULIP<br /> www.nature.com</p><p>https://www.nature.com/articles/s41598-017-03996-z<br /> Example gallery of NanoPlot &ndash; Gigabase or gigabyte</p><p>https://gigabaseorgigabyte.wordpress.com/2017/06/01/example-gallery-of-nanoplot/<br /> Tool documentation</p><p>https://broadinstitute.github.io/picard/command-line-overview.html<br /> Chromosome-scale scaffolding of de novo genome assemblies based on chromatin interactions. - PubMed - NCBI</p><p>https://www.ncbi.nlm.nih.gov/pubmed/24185095<br /> MAFFT ver.7 - a multiple sequence alignment program</p><p>https://mafft.cbrc.jp/alignment/software/algorithms/algorithms.html<br /> Measuring the distance between multiple sequence alignments | Bioinformatics | Oxford Academic</p><p>https://academic.oup.com/bioinformatics/article/28/4/495/212883<br /> The MUMmer 3 examples</p><p>http://mummer.sourceforge.net/examples/<br /> MAFFT ver.7 - a multiple sequence alignment program</p><p>https://mafft.cbrc.jp/alignment/software/tips.html<br /> Omega | Overlap-graph de novo Assembler for Metagenomics</p><p>https://omega.omicsbio.org/<br /> abiswas-odu/Disco: Multi-threaded Distributed Memory Overlap-Layout-Consensus (OLC) Metagenome Assembler</p><p>https://github.com/abiswas-odu/Disco<br /> SAGE: String-overlap Assembly of GEnomes | BMC Bioinformatics | Full Text</p><p>https://bmcbioinformatics.biomedcentral.com/articles/10.1186/1471-2105-15-302</p><p>Fast and sensitive mapping of nanopore sequencing reads with GraphMap | Nature Communications</p><p>https://www.nature.com/articles/ncomms11307<br /> lumpy-sv/extractSplitReads_BwaMem at master &middot; arq5x/lumpy-sv</p><p>https://github.com/arq5x/lumpy-sv/blob/master/scripts/extractSplitReads_BwaMem<br /> jts/nanocorrect: Experimental pipeline for correcting nanopore reads</p><p>https://github.com/jts/nanocorrect</p><p>video - how to install flash plugin on ubuntu 14.04 LTS 64-bit version - Ask Ubuntu</p><p>https://askubuntu.com/questions/469553/how-to-install-flash-plugin-on-ubuntu-14-04-lts-64-bit-version<br /> lh3/fermi: A WGS de novo assembler based on the FMD-index for large genomes</p><p>https://github.com/lh3/fermi<br /> Multi-metagenome</p><p>http://madsalbertsen.github.io/multi-metagenome/docs/step9.html<br /> Bandage by rrwick</p><p>https://rrwick.github.io/Bandage/<br /> Codon Optimization OnLine (COOL): a web-based multi-objective optimization platform for synthetic gene design | Bioinformatics | Oxford Academic</p><p>https://academic.oup.com/bioinformatics/article/30/15/2210/2391162<br /> Genome Architecture and Evolution of a Unichromosomal Asexual Nematode - ScienceDirect</p><p>https://www.sciencedirect.com/science/article/pii/S096098221731076X?via%3Dihub#fig4<br /> How to determine chimeras in my de novo assembly? - SEQanswers</p><p>http://seqanswers.com/forums/showthread.php?t=26721<br /> samtools(1) manual page</p><p>http://www.htslib.org/doc/samtools.html<br /> How To Filter Mapped Reads With Samtools</p><p>https://www.biostars.org/p/56246/<br /> The MUMmer 3 manual</p><p>http://mummer.sourceforge.net/manual/#nucmer<br /> assembly_olc.pdf</p><p>http://www.cs.jhu.edu/~langmea/resources/lecture_notes/assembly_olc.pdf<br /> SAM and BAM filtering oneliners</p><p>https://gist.github.com/davfre/8596159<br /> Inroduction to dot-plots</p><p>http://www.code10.info/index.php%3Foption%3Dcom_content%26view%3Darticle%26id%3D64:inroduction-to-dot-plots%26catid%3D52:cat_coding_algorithms_dot-plots%26Itemid%3D76<br /> RepeatFinder Home Page</p><p>http://www.cbcb.umd.edu/software/RepeatFinder/<br /> RepeatFinderReprint.pdf</p><p>http://www.cbcb.umd.edu/software/RepeatFinder/RepeatFinderReprint.pdf<br /> https://bernatgel.github.io/karyoploter_tutorial//Tutorial/CreateIdeogram/CreateIdeogram.html</p><p>https://bernatgel.github.io/karyoploter_tutorial//Tutorial/CreateIdeogram/CreateIdeogram.html<br /> Circular Visualization in R</p><p>http://zuguang.de/circlize_book/book/introduction.html#a-qiuck-glance<br /> Creating a coverage plot using BEDTools and R</p><p>https://davetang.org/muse/2015/08/05/creating-a-coverage-plot-using-bedtools-and-r/<br /> Eval: A software package for analysis of genome annotations | BMC Bioinformatics | Full Text</p><p>https://bmcbioinformatics.biomedcentral.com/articles/10.1186/1471-2105-4-50<br /> eval-documentation.pdf</p><p>http://mblab.wustl.edu/media/software/eval-documentation.pdf<br /> OmicCircos: A Simple-to-Use R Package for the Circular Visualization of Multidimensional Omics Data</p><p>https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3921174/<br /> sequence - download.tardigrades.org &gt; v1 &gt; sequence</p><p>http://download.tardigrades.org/v1/sequence/<br /> ksahlin/BESST: BESST - scaffolder for genomic assemblies</p><p>https://github.com/ksahlin/BESST<br /> reubwn/scripts: Useful scripts for various things</p><p>https://github.com/reubwn/scripts<br /> ICEberg</p><p>http://db-mml.sjtu.edu.cn/ICEberg/index.php<br /> Satsuma - Evolution and Genomics</p><p>http://evomics.org/learning/genomics/satsuma/<br /> A complete bacterial genome assembled de novo using only nanopore sequencing data | Nature Methods</p><p>https://www.nature.com/articles/nmeth.3444<br /> vezzi/FRC_align: Computes FRC from SAM/BAM file and not from afg files</p><p>https://mail.google.com/mail/u/0/#inbox<br /> Read GTF file into R - Dave Tang's blog</p><p>https://davetang.org/muse/2017/08/04/read-gtf-file-r/</p><p>https://bernatgel.github.io/karyoploter_tutorial//Tutorial/CustomGenomes/CustomGenomes.html</p><p>https://bernatgel.github.io/karyoploter_tutorial//Tutorial/CustomGenomes/CustomGenomes.html<br /> Dot: Interactive dot plot for genome-genome alignments</p><p>https://dnanexus.github.io/dot/<br /> Zoho Accounts</p><p>https://accounts.zoho.eu/signin?servicename=ZohoProjects&amp;serviceurl=https%3A%2F%2Fprojects.zoho.eu%2Fportal%2Favaga2<br /> lh3/minimap2: A versatile pairwise aligner for genomic and spliced nucleotide sequences</p><p>https://github.com/lh3/minimap2<br /> SSPACE-LongRead: scaffolding bacterial draft genomes using long read sequence information | BMC Bioinformatics | Full Text</p><p>https://bmcbioinformatics.biomedcentral.com/articles/10.1186/1471-2105-15-211<br /> Palindromic gene amplification &mdash; an evolutionarily conserved role for DNA inverted repeats in the genome | Nature Reviews Cancer</p><p>https://www.nature.com/articles/nrc2591<br /> bioinformatics - BLAST DNA Sequences Reversed - Biology Stack Exchange</p><p>https://biology.stackexchange.com/questions/8160/blast-dna-sequences-reversed<br /> LASTZ</p><p>http://www.bx.psu.edu/miller_lab/dist/README.lastz-1.02.00/README.lastz-1.02.00a.html<br /> SOGo - (1652) Inbox</p><p>https://sogo.unamur.be/SOGo/so/jnarayan/Mail/view<br /> Tetra-Nucleotide Analysis (TNA) | BIOiPLUG Help center</p><p>http://help.bioiplug.com/tetra-nucleotide-analysis-tna/</p><p>Clustering metagenomic contigs on tetranucleotide frequency &mdash; CGAT documentation</p><p>http://cgat.readthedocs.io/en/latest/recipes/metagenome_contigs_kmers.html</p><p>&nbsp;</p>]]></description>
	<dc:creator>Jit</dc:creator>
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