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	<title><![CDATA[BOL: Related items]]></title>
	<link>https://bioinformaticsonline.com/related/2791?offset=50</link>
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	<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/news/view/44515/cleaner-blast-databases-for-more-accurate-results</guid>
	<pubDate>Tue, 23 Apr 2024 01:23:08 -0500</pubDate>
	<link>https://bioinformaticsonline.com/news/view/44515/cleaner-blast-databases-for-more-accurate-results</link>
	<title><![CDATA[Cleaner BLAST Databases for More Accurate Results]]></title>
	<description><![CDATA[<p>Do you use&nbsp;<a href="https://blast.ncbi.nlm.nih.gov/Blast.cgi?utm_source=ncbi_insights&amp;utm_medium=referral&amp;utm_campaign=blast-cleaner-20240422">BLAST</a><span style="font-size: 12.8px; font-weight: normal;">&nbsp;to identify a sequence or the evolutionary scope of a gene? That can be challenging if contaminated and misclassified sequences are in the BLAST databases and show up in your search results. To address</span><span style="font-size: 12.8px; font-weight: normal;">&nbsp;this problem</span><span style="font-size: 12.8px; font-weight: normal;">, we now use the NCBI quality assurance tools listed below to systematically remove these misleading sequences from the default nucleotide (nt) and protein (nr) BLAST databases.</span><span style="font-size: 12.8px; font-weight: normal;">&nbsp;</span></p><div><ul>
<li><a href="https://github.com/ncbi/fcs">Foreign Contamination Screen tool for genome cross-species screening (FCS-GX)</a>&nbsp;detects contamination from foreign organisms in genomes and other sequences using the genome cross-species aligner (GX)&nbsp;</li>
<li><a href="https://ncbiinsights.ncbi.nlm.nih.gov/2022/05/27/ani-for-assembly-validation?utm_source=ncbi_insights&amp;utm_medium=referral&amp;utm_campaign=blast-cleaner-20240422">Average Nucleotide Identity (ANI)</a>&nbsp;evaluates the taxonomic classification of prokaryotic genome assemblies. Sequences from genomes marked up as &lsquo;unverified source organism&rsquo; are considered suspect and removed.&nbsp;</li>
</ul><p>Ref&nbsp;https://ncbiinsights.ncbi.nlm.nih.gov/2024/04/22/cleaner-blast-databases-more-accurate-results/</p></div>]]></description>
	<dc:creator>LEGE</dc:creator>
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	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/38381/repeatmasker-compatible-blast-tool</guid>
	<pubDate>Fri, 07 Dec 2018 08:13:03 -0600</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/38381/repeatmasker-compatible-blast-tool</link>
	<title><![CDATA[RepeatMasker compatible blast tool]]></title>
	<description><![CDATA[<p><span>RMBlast is a RepeatMasker compatible version of the standard NCBI blastn program. The primary difference between this distribution and the NCBI distribution is the addition of a new program "rmblastn" for use with RepeatMasker and RepeatModeler.</span></p>
<p>RMBlast supports RepeatMasker searches by adding a few necessary features to the stock NCBI blastn program. These include:</p>
<ul>
<li>Support for custom matrices ( without KA-Statistics ).</li>
<li>Support for cross_match-like complexity adjusted scoring. Cross_match is Phil Green's seeded smith-waterman search algorithm.</li>
<li>Support for cross_match-like masklevel filtering.</li>
</ul>
<p>https://anaconda.org/bioconda/rmblast</p><p>Address of the bookmark: <a href="http://www.repeatmasker.org/RMBlast.html" rel="nofollow">http://www.repeatmasker.org/RMBlast.html</a></p>]]></description>
	<dc:creator>Neel</dc:creator>
</item>
<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/43985/visualise-blast-results</guid>
	<pubDate>Tue, 11 Oct 2022 03:15:10 -0500</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/43985/visualise-blast-results</link>
	<title><![CDATA[Visualise blast results !]]></title>
	<description><![CDATA[<p>Kablammo helps you create interactive visualizations of BLAST results from your web browser. Find your most interesting alignments, list detailed parameters for each, and export a publication-ready vector image, all without installing any software.</p>
<p>&nbsp;</p><p>Address of the bookmark: <a href="https://kablammo.wasmuthlab.org/" rel="nofollow">https://kablammo.wasmuthlab.org/</a></p>]]></description>
	<dc:creator>Abhi</dc:creator>
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	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/38449/koala-keggs-internal-annotation-tool-for-k-number-assignment-of-kegg-genes-using-ssearch-computation</guid>
	<pubDate>Wed, 12 Dec 2018 09:16:55 -0600</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/38449/koala-keggs-internal-annotation-tool-for-k-number-assignment-of-kegg-genes-using-ssearch-computation</link>
	<title><![CDATA[KOALA: KEGG&#039;s internal annotation tool for K number assignment of KEGG GENES using SSEARCH computation]]></title>
	<description><![CDATA[<p>KOALA (KEGG Orthology And Links Annotation) is KEGG's internal annotation tool for&nbsp;<a href="https://www.kegg.jp/kegg/ko.html">K number</a>&nbsp;assignment of KEGG GENES using SSEARCH computation. BlastKOALA and GhostKOALA assign K numbers to the user's sequence data by&nbsp;<a href="http://www.ncbi.nlm.nih.gov/blast/">BLAST</a>&nbsp;and&nbsp;<a href="http://www.bi.cs.titech.ac.jp/ghostx/">GHOSTX</a>&nbsp;searches, respectively, against a nonredundant set of KEGG GENES. Annotate Sequence in KEGG Mapper and Pathogen Checker in KEGG Pathogen are special interfaces to the BlastKOALA server and can be executed in an interactive mode. &nbsp;&nbsp; See&nbsp;<a href="https://www.kegg.jp/blastkoala/help_blastkoala.html" target="_blastkoala">Step-by-step Instructions</a>.</p>
<div>Reference: Kanehisa, M., Sato, Y., and Morishima, K. (2016) BlastKOALA and GhostKOALA: KEGG tools for functional characterization of genome and metagenome sequences. J. Mol. Biol. 428, 726-731. [<a href="http://www.ncbi.nlm.nih.gov/pubmed/26585406">pubmed</a>] [<a href="https://doi.org/10.1016/j.jmb.2015.11.006">pdf</a>]</div><p>Address of the bookmark: <a href="https://www.kegg.jp/blastkoala/" rel="nofollow">https://www.kegg.jp/blastkoala/</a></p>]]></description>
	<dc:creator>Abhimanyu Singh</dc:creator>
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	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/38659/detail-annotation-of-genes</guid>
	<pubDate>Fri, 11 Jan 2019 05:23:33 -0600</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/38659/detail-annotation-of-genes</link>
	<title><![CDATA[Detail annotation of genes !]]></title>
	<description><![CDATA[<p>gene_info recalculated daily<br>---------------------------------------------------------------------------<br> tab-delimited<br> one line per GeneID<br> Column header line is the first line in the file.<br> Note: subsets of gene_info are available in the DATA/GENE_INFO<br> directory (described later)<br>---------------------------------------------------------------------------</p>
<p>tax_id:<br> the unique identifier provided by NCBI Taxonomy<br> for the species or strain/isolate</p>
<p>GeneID:<br> the unique identifier for a gene<br> ASN1: geneid</p>
<p>Symbol:<br> the default symbol for the gene<br> ASN1: gene-&gt;locus</p>
<p>LocusTag:<br> the LocusTag value<br> ASN1: gene-&gt;locus-tag</p>
<p>Synonyms:<br> bar-delimited set of unofficial symbols for the gene</p>
<p>dbXrefs:<br> bar-delimited set of identifiers in other databases<br> for this gene. The unit of the set is database:value.<br> Note that HGNC and MGI include 'HGNC' and 'MGI', respectively,<br> in the value part of their identifier. Consequently,<br> dbXrefs for these databases will appear like:<br> HGNC:HGNC:1100<br> This would be interpreted as database='HGNC', value='HGNC:1100'<br> Example for MGI:<br> MGI:MGI:104537<br> This would be interpreted as database='MGI', value='MGI:104537'</p>
<p>chromosome:<br> the chromosome on which this gene is placed.<br> for mitochondrial genomes, the value 'MT' is used.</p>
<p>map location:<br> the map location for this gene</p>
<p>description:<br> a descriptive name for this gene</p>
<p>type of gene:<br> the type assigned to the gene according to the list of options<br> provided in https://www.ncbi.nlm.nih.gov/IEB/ToolBox/CPP_DOC/lxr/source/src/objects/entrezgene/entrezgene.asn</p>
<p><br>Symbol from nomenclature authority:<br> when not '-', indicates that this symbol is from a<br> a nomenclature authority</p>
<p>Full name from nomenclature authority:<br> when not '-', indicates that this full name is from a<br> a nomenclature authority</p>
<p>Nomenclature status:<br> when not '-', indicates the status of the name from the <br> nomenclature authority (O for official, I for interim)</p>
<p>Other designations:<br> pipe-delimited set of some alternate descriptions that<br> have been assigned to a GeneID<br> '-' indicates none is being reported.</p>
<p>Modification date:<br> the last date a gene record was updated, in YYYYMMDD format</p>
<p>Feature type:<br> pipe-delimited set of annotated features and their classes or <br> controlled vocabularies, displayed as feature_type:feature_class <br> or feature_type:controlled_vocabulary, when appropriate; derived <br> from select feature annotations on RefSeq(s) associated with the <br> GeneID</p><p>Address of the bookmark: <a href="ftp://ftp.ncbi.nih.gov/gene/DATA/GENE_INFO/" rel="nofollow">ftp://ftp.ncbi.nih.gov/gene/DATA/GENE_INFO/</a></p>]]></description>
	<dc:creator>Jit</dc:creator>
</item>
<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/news/view/5887/pubmed-opens-for-comment</guid>
	<pubDate>Thu, 24 Oct 2013 12:40:17 -0500</pubDate>
	<link>https://bioinformaticsonline.com/news/view/5887/pubmed-opens-for-comment</link>
	<title><![CDATA[PubMed opens for comment]]></title>
	<description><![CDATA[<p>The informal conversations that researchers have at scientific meetings look set to move online, if a new initiative by the US National Center for Biotechnology Information (NCBI) has its way. On 22 October, the NCBI of Bethesda, Maryland, launched the pilot phase of a programme called PubMed Commons. This will allow users to comment on published abstracts on the PubMed website, which indexes some 22 million papers.<br /><br />For now, only a select group of researchers and their invited guests can use the system. But the NCBI's director David Lipman, who helped to develop the programme, says that soon any PubMed author will be allowed to comment under his or her real name and anyone will be able to read the comments.</p><p>More @ <a href="http://www.nature.com/news/pubmed-opens-for-comment-1.14023">http://www.nature.com/news/pubmed-opens-for-comment-1.14023</a></p><p>News source Nature.</p>]]></description>
	<dc:creator>Rahul Nayak</dc:creator>
</item>
<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/videolist/watch/16685/webinar-blast-in-the-cloud</guid>
	<pubDate>Mon, 15 Sep 2014 17:29:32 -0500</pubDate>
	<link>https://bioinformaticsonline.com/videolist/watch/16685/webinar-blast-in-the-cloud</link>
	<title><![CDATA[Webinar: BLAST in the Cloud]]></title>
	<description><![CDATA[<iframe width="" height="" src="https://www.youtube-nocookie.com/embed/wLm-RfdcvnU" frameborder="0" allowfullscreen></iframe>Presented July 30, 2014 and covering: an NCBI BLAST AMI at Amazon Web Services; introduction to AWS and setting up an instance; running command line BLAST and using the BLAST URL API via the AMI; and answers to attendee questions.]]></description>
	
</item>
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	<guid isPermaLink="true">https://bioinformaticsonline.com/news/view/27344/orffinder-with-smart-blast</guid>
	<pubDate>Tue, 17 May 2016 01:43:15 -0500</pubDate>
	<link>https://bioinformaticsonline.com/news/view/27344/orffinder-with-smart-blast</link>
	<title><![CDATA[ORFfinder with smart BLAST]]></title>
	<description><![CDATA[<p><span>ORF Finder</span></p><p><span><a href="http://www.ncbi.nlm.nih.gov/orffinder">ORFfinder</a><span>&nbsp;is a graphical analysis tool for finding open reading frames (ORFs). We&rsquo;ve been working on a few updates, and we&rsquo;d like to find out what you think about them. Read on to find out what you can do with the new ORFfinder.</span></span></p><p>Smart BLAST (https://ncbiinsights.ncbi.nlm.nih.gov/2015/07/29/smartblast/)</p><p>Select one or a group of ORFs and BLAST several databases at once, and use the newly developed&nbsp;<a href="http://blast.ncbi.nlm.nih.gov/smartblast/">SmartBLAST</a>&nbsp;to verify protein names.&nbsp;Looking for the traditional results from&nbsp;<a href="http://blast.ncbi.nlm.nih.gov/Blast.cgi">BLAST</a>? They&rsquo;re there too.</p>]]></description>
	<dc:creator>Jit</dc:creator>
</item>
<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/31123/biodownloader</guid>
	<pubDate>Sat, 25 Feb 2017 17:52:33 -0600</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/31123/biodownloader</link>
	<title><![CDATA[BioDownloader]]></title>
	<description><![CDATA[<p><strong><em>BioDownloader</em></strong> is a program for downloading and/or updating files from ftp/http servers. The program has unique features that are specifically designed to deal with bioinformatics data files and servers:</p>
<ul>
<li>optimized to work with vast amount of data and very large file sets (~ 10,000 - 100,000).</li>
<li>allows the selective retrieval of only the required files (file masks, ls-lR parsing, recursive search, updates)</li>
<li>has a built-in repository containing the settings for the most common bioinformatics download needs</li>
<li>built-in wizard for batch post-processing of downloaded files (archive extraction, file conversion, etc.)</li>
<li>capable of performing multiple download or update tasks simultaneously</li>
</ul>
<p>BioDownloader has a built-in repository containing the settings for common bioinformatics file-synchronization needs, including the Protein Data Bank (PDB) and National Center for Biotechnology Information (NCBI) databases. It can post-process downloaded files, including archive extraction and file conversions.</p>
<p>http://dunbrack.fccc.edu/BioDownloader/</p><p>Address of the bookmark: <a href="http://dunbrack.fccc.edu/BioDownloader/" rel="nofollow">http://dunbrack.fccc.edu/BioDownloader/</a></p>]]></description>
	<dc:creator>Surabhi Chaudhary</dc:creator>
</item>
<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/news/view/38226/ncbi-to-assist-in-virus-hunting-data-science-hackathon</guid>
	<pubDate>Thu, 15 Nov 2018 12:55:01 -0600</pubDate>
	<link>https://bioinformaticsonline.com/news/view/38226/ncbi-to-assist-in-virus-hunting-data-science-hackathon</link>
	<title><![CDATA[NCBI to assist in Virus Hunting Data Science Hackathon]]></title>
	<description><![CDATA[<p>NCBI Hackathon are pleased to announce the second installment of the&nbsp;<a href="https://ncbiinsights.ncbi.nlm.nih.gov/2017/11/30/ncbi-southern-california-genomics-hackathon-january/" target="_blank">SoCal Bioinformatics Hackathon</a>. From January 9-11, 2019, the&nbsp;<a href="https://www.ncbi.nlm.nih.gov/" target="_blank">NCBI</a>&nbsp;will help run a bioinformatics hackathon in Southern California hosted by the&nbsp;<a href="http://www.csrc.sdsu.edu/" target="_blank">Computational Sciences Research Center</a>&nbsp;at&nbsp;<a href="http://www.sdsu.edu/" target="_blank">San Diego State University</a>!</p><p><span>NCBI Hackathon</span>&nbsp;specifically looking for folks who have experience in computational virus hunting or adjacent fields to identify known, taxonomically-definable and novel viruses from a few hundred thousand metagenomic datasets that we&rsquo;ll put on cloud infrastructure. This event is for researchers, including students and postdocs, who are already engaged in the use of bioinformatics data or in the development of pipelines for virological analyses from high-throughput experiments. If this describes you, please&nbsp;<a href="https://goo.gl/forms/kDnSG0IAZD62XQRe2" target="_blank">apply</a>! The event is open to anyone selected for the hackathon and willing to travel to SDSU (see below).</p><p>https://ncbiinsights.ncbi.nlm.nih.gov/2018/11/09/ncbi-sdsu-virus-hunting-data-science-hackathon-january-2019/</p>]]></description>
	<dc:creator>BioStar</dc:creator>
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