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	<title><![CDATA[BOL: Related items]]></title>
	<link>https://bioinformaticsonline.com/related/27967?offset=680</link>
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	<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/37233/rna-seq-analysis-workshop-course-materials</guid>
	<pubDate>Tue, 03 Jul 2018 08:14:14 -0500</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/37233/rna-seq-analysis-workshop-course-materials</link>
	<title><![CDATA[RNA-seq Analysis Workshop Course Materials]]></title>
	<description><![CDATA[RNAseq can be roughly divided into two "types":

Reference genome-based - an assembled genome exists for a species for which an RNAseq experiment is performed. It allows reads to be aligned against the reference genome and significantly improves our ability to reconstruct transcripts. This category would obviously include humans and most model organisms but excludes the majority of truly biologically intereting species (e.g., Hyacinth macaw);

Reference genome-free - no genome assembly for the species of interest is available. In this case one would need to assemble the reads into transcripts using de novo approaches. This type of RNAseq is as much of an art as well as science because assembly is heavily parameter-dependent and difficult to do well.
In this lesson we will focus on the Reference genome-based type of RNA seq.

http://chagall.med.cornell.edu/RNASEQcourse/<p>Address of the bookmark: <a href="http://chagall.med.cornell.edu/RNASEQcourse/" rel="nofollow">http://chagall.med.cornell.edu/RNASEQcourse/</a></p>]]></description>
	<dc:creator>Jit</dc:creator>
</item>

<item>
  <guid isPermaLink='true'>https://bioinformaticsonline.com/opportunity/view/22615/jrf-position-%E2%80%93-bioinformatics-department-aravind-medical-research-foundation-amrf-madurai</guid>
  <pubDate>Fri, 12 Jun 2015 05:42:12 -0500</pubDate>
  <link></link>
  <title><![CDATA[JRF Position – Bioinformatics Department, Aravind Medical Research Foundation (AMRF), Madurai.]]></title>
  <description><![CDATA[
<p>Applications are invited from eligible candidates for the post of Junior Research Fellow (JRF) to work at the Department of Bioinformatics, Aravind Medical Research Foundation in the following DST-SERB funded project “Clinical exome analysis pipeline for eye disease next-generation sequencing panel”.</p>

<p>Post: Junior Research Fellow (1 Position)</p>

<p>Duration: Three years</p>

<p>Qualification: First class in M.Sc/M.tech in Bioinformatics/Life Sciences/Biophysics/ Biostatistics/Bioengineering. Experience in Database development, NGS data analysis, Systems Biology and Structural Bioinformatics is desired. Preference will be given to the candidates with good computer programming skills in C, C++, R, Perl, PHP, Unix Scripting etc.</p>

<p>Selected candidates will be paid fellowship as per existing DST norms.</p>

<p>How to apply:</p>

<p>Candidates are requested to apply through one of the two modes given below<br />1. Online application – Click here to submit the online application https://docs.google.com/forms/d/16h2GLnQ-Ny-tLtlgfY3Bx3sCjeHJE30cfhJaDqW_uRs/viewform?c=0&amp;w=1<br />2. Application forms can be downloaded from here.https://docs.google.com/file/d/0BwwJEudQStxFWXdNWXl4NWtDaWc/edit<br /> Filled in application form should be sent by post to Dr. D. Bharanidharan, Department of Bioinformatics, Aravind Medical Research Foundation No 1, Anna Nagar Madurai – 625 020,</p>

<p>Candidates should apply by online or submit their applications by post on or before 15th June, 2015. Only Short listed candidates will be called for an interview. No TA/DA will be paid.</p>
]]></description>
</item>
<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/37502/alignqc-a-tool-for-assessing-an-alignment-and-generating-reports-that-are-easy-to-share</guid>
	<pubDate>Tue, 07 Aug 2018 04:41:07 -0500</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/37502/alignqc-a-tool-for-assessing-an-alignment-and-generating-reports-that-are-easy-to-share</link>
	<title><![CDATA[AlignQC: A tool for assessing an alignment, and generating reports that are easy to share]]></title>
	<description><![CDATA[<p><span>Long read alignment analysis. Generate a reports on sequence alignments for mappability vs read sizes, error patterns, annotations and rarefraction curve analysis. The most basic analysis only requires a BAM file, and outputs a web browser compatible xhtml to visualize/share/store/extract analysis results.</span></p>
<p>https://f1000research.com/articles/6-100/</p>
<p>https://github.com/jason-weirather/AlignQC</p><p>Address of the bookmark: <a href="https://www.healthcare.uiowa.edu/labs/au/AlignQC/" rel="nofollow">https://www.healthcare.uiowa.edu/labs/au/AlignQC/</a></p>]]></description>
	<dc:creator>Jit</dc:creator>
</item>
<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/pages/view/22571/pattern-matching-problem-solution-with-perl</guid>
	<pubDate>Tue, 09 Jun 2015 23:58:45 -0500</pubDate>
	<link>https://bioinformaticsonline.com/pages/view/22571/pattern-matching-problem-solution-with-perl</link>
	<title><![CDATA[Pattern Matching Problem Solution with Perl]]></title>
	<description><![CDATA[<p>Problem at http://rosalind.info/problems/1c/</p><p>#Find all occurrences of a pattern in a string.<br />#Given: Strings Pattern and Genome.<br />#Return: All starting positions in Genome where Pattern appears as a substring. Use 0-based indexing.<br /><br />use strict;<br />use warnings;<br /><br />my $string="GATATATGCATATACTT";<br />my $subStr="ATAT";<br />my $kmer=length($subStr);<br /><br />kmerMatch ($string, $subStr, $kmer);<br /><br />sub kmerMatch { #Check the exact matching kmers with sliding window<br />my ($string, $myStr, $kmer)=@_;<br />for (my $aa=0; $aa&lt;=(length($string)-$kmer); $aa++) {<br />&nbsp;&nbsp;&nbsp; my $myWin=substr&nbsp; $string, $aa,$kmer;<br />&nbsp;&nbsp;&nbsp; if ($myWin eq $myStr) {<br />&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; #print "$myWin eq $myStr\n";<br />&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; print $aa;<br />&nbsp;&nbsp;&nbsp; }<br />}<br />}</p>]]></description>
	<dc:creator>Jit</dc:creator>
</item>
<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/37993/platypus-a-haplotype-based-variant-caller-for-next-generation-sequence-data</guid>
	<pubDate>Thu, 25 Oct 2018 06:14:55 -0500</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/37993/platypus-a-haplotype-based-variant-caller-for-next-generation-sequence-data</link>
	<title><![CDATA[Platypus: A Haplotype-Based Variant Caller For Next Generation Sequence Data]]></title>
	<description><![CDATA[<p><strong>Platypus</strong><span>&nbsp;is a tool designed for efficient and accurate variant-detection in high-throughput sequencing data. By using local realignment of reads and local assembly it achieves both high sensitivity and high specificity. Platypus can detect SNPs, MNPs, short indels, replacements and (using the assembly option) deletions up to several kb. It has been extensively tested on&nbsp;</span><a href="http://www.ncbi.nlm.nih.gov/pubmed/?term=24463883">whole-genome</a><span>,&nbsp;</span><a href="http://www.nature.com/ng/journal/v45/n1/abs/ng.2492.html">exon-capture</a><span>, and&nbsp;</span><a href="http://www.nature.com/nature/journal/v493/n7432/abs/nature11725.html">targeted capture</a><span>&nbsp;data, it has been run on very large datasets as part of the&nbsp;</span><a href="http://www.1000genomes.org/">Thousand Genomes</a><span>&nbsp;and WGS500 projects, and is being used in clinical sequencing trials in the&nbsp;</span><a href="http://www.mcgprogramme.com/">Mainstreaming Cancer Genetics</a><span>&nbsp;programme.&nbsp;</span></p>
<p><span>Tutorial&nbsp;https://github.com/andyrimmer/Platypus/blob/master/misc/README.txt</span></p><p>Address of the bookmark: <a href="http://www.well.ox.ac.uk/platypus" rel="nofollow">http://www.well.ox.ac.uk/platypus</a></p>]]></description>
	<dc:creator>Shruti Paniwala</dc:creator>
</item>
<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/news/view/22769/ensembl-27</guid>
	<pubDate>Tue, 16 Jun 2015 16:10:36 -0500</pubDate>
	<link>https://bioinformaticsonline.com/news/view/22769/ensembl-27</link>
	<title><![CDATA[Ensembl 27]]></title>
	<description><![CDATA[<h3>What is new?</h3><ul>
<li>Expansion of Protists and Fungi with hundreds of annotated genomes</li>
<li>Variation data for bread wheat, rice, <em>Aedes aegypti</em>, and <em>Ixodes scapularis</em></li>
<li>Whole genome alignments for <em>O. longistaminata</em> and <em>T. cacao</em></li>
<li>Non-coding RNA gene models in <a href="http://bacteria.ensembl.org">Bacteria</a></li>
<li>New assembly of tomato (version 2.50)</li>
<li>Full support for UCSC Track Hub format for hosting your own data in Ensembl</li>
</ul><p>More at http://www.ensembl.info/blog/2015/06/16/ensembl-genomes-release-27-is-out/</p>]]></description>
	<dc:creator>Jit</dc:creator>
</item>
<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/39441/snakepipes-a-toolkit-based-on-snakemake-and-python-for-analysis-of-ngs-data</guid>
	<pubDate>Thu, 30 May 2019 04:06:13 -0500</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/39441/snakepipes-a-toolkit-based-on-snakemake-and-python-for-analysis-of-ngs-data</link>
	<title><![CDATA[snakepipes: A toolkit based on snakemake and python for analysis of NGS data]]></title>
	<description><![CDATA[<p><span><span>snakePipes are flexible and powerful workflows built using&nbsp;</span><a href="https://github.com/maxplanck-ie/snakepipes/blob/master/snakemake.readthedocs.io">snakemake</a><span>&nbsp;that simplify the analysis of NGS data.</span></span></p>
<ul>
<li>DNA-mapping*</li>
<li>ChIP-seq*</li>
<li>RNA-seq*</li>
<li>ATAC-seq*</li>
<li>scRNA-seq</li>
<li>Hi-C</li>
<li>Whole Genome Bisulfite Seq/WGBS</li>
</ul>
<p><span>(*Also available in "allele-specific" mode)</span></p>
<p><span>snakePipes can be installed via conda : </span></p>
<p><span>'conda install -c mpi-ie -c bioconda -c conda-forge snakePipes'. </span></p>
<p><span>Source code (</span><a href="https://github.com/maxplanck-ie/snakepipes" target="">https://github.com/maxplanck-ie/snakepipes</a><span>) and documentation (</span><a href="https://snakepipes.readthedocs.io/en/latest/" target="">https://snakepipes.readthedocs.io/en/latest/</a><span>) are available online.</span></p><p>Address of the bookmark: <a href="https://github.com/maxplanck-ie/snakepipes" rel="nofollow">https://github.com/maxplanck-ie/snakepipes</a></p>]]></description>
	<dc:creator>Jit</dc:creator>
</item>

<item>
  <guid isPermaLink='true'>https://bioinformaticsonline.com/opportunity/view/22780/ra-bioinformatics-at-institution-centre-for-human-genetics-bangalore</guid>
  <pubDate>Wed, 17 Jun 2015 19:14:37 -0500</pubDate>
  <link></link>
  <title><![CDATA[RA Bioinformatics at Institution: Centre for Human Genetics,  Bangalore]]></title>
  <description><![CDATA[
<p>Institution: Centre for Human Genetics, <br />Bangalore <br />Discipline: Molecular Genetics of Human Disease Biology </p>

<p>Minimum qualification: MSc in any branch of life sciences</p>

<p>Applications are invited for the position of a Research Assistant in the Centre for Human Genetics, Bangalore. </p>

<p>The project involves identification of mutations in MPS (mucopolysaccharidosis) patients, and study of their predicted effects to understand how the mutations lead to disease. </p>

<p>Techniques used will be genomic DNA isolation, PCR, DNA sequencing and sequence analysis. Computational tools would also be used to analyse and interpret data. </p>

<p>Candidates may be assigned work in the ongoing project or in new ones. </p>

<p>The candidate who is selected and joins would acquire hands-on experience in research and the capability to conduct insightful research. </p>

<p>Candidates applying for the position should have an MSc in any branch of life sciences. Those with research experience in cell and molecular biology, and high NET/ GATE score would be preferred. </p>

<p>The successful applicant is expected to stay for at least one and a half years. </p>

<p>Please apply with CV to Sudha Srinivasan (sudha@ibab.ac.in), stating where you saw this ad.</p>
]]></description>
</item>
<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/40604/gapfinisher-a-reliable-gap-filling-pipeline-for-sspace-longread-scaffolder-output</guid>
	<pubDate>Fri, 24 Jan 2020 06:04:40 -0600</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/40604/gapfinisher-a-reliable-gap-filling-pipeline-for-sspace-longread-scaffolder-output</link>
	<title><![CDATA[gapFinisher: A reliable gap filling pipeline for SSPACE-LongRead scaffolder output]]></title>
	<description><![CDATA[<p><span>gapFinisher is based on the controlled use of a previously published gap filling tool FGAP and works on all standard Linux/UNIX command lines. They compare the performance of gapFinisher against two other published gap filling tools PBJelly and GMcloser. </span></p>
<p><span>gapFinisher can fill gaps in draft genomes quickly and reliably.</span></p><p>Address of the bookmark: <a href="https://github.com/kammoji/gapFinisher" rel="nofollow">https://github.com/kammoji/gapFinisher</a></p>]]></description>
	<dc:creator>Rahul Nayak</dc:creator>
</item>
<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/41730/parliament2-runs-a-combination-of-tools-to-generate-structural-variant-calls-on-whole-genome-sequencing-data</guid>
	<pubDate>Thu, 28 May 2020 21:57:03 -0500</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/41730/parliament2-runs-a-combination-of-tools-to-generate-structural-variant-calls-on-whole-genome-sequencing-data</link>
	<title><![CDATA[Parliament2: Runs a combination of tools to generate structural variant calls on whole-genome sequencing data]]></title>
	<description><![CDATA[<p>Parliament2 identifies structural variants in a given sample relative to a reference genome. These structural variants cover large deletion events that are called as Deletions of a region, Insertions of a sequence into a region, Duplications of a region, Inversions of a region, or Translocations between two regions in the genome.</p>
<p>Parliament2 runs a combination of tools to generate structural variant calls on whole-genome sequencing data. It can run the following callers: Breakdancer, Breakseq2, CNVnator, Delly2, Manta, and Lumpy. Because of synergies in how the programs use computational resources, these are all run in parallel. Parliament2 will produce the outputs of each of the tools for subsequent investigation.</p><p>Address of the bookmark: <a href="https://github.com/dnanexus/parliament2" rel="nofollow">https://github.com/dnanexus/parliament2</a></p>]]></description>
	<dc:creator>Jit</dc:creator>
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