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	<title><![CDATA[BOL: Related items]]></title>
	<link>https://bioinformaticsonline.com/related/27973?offset=720</link>
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	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/42485/fastprongs-fast-preprocessing-of-next-generation-sequencing-reads</guid>
	<pubDate>Sat, 26 Dec 2020 08:35:21 -0600</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/42485/fastprongs-fast-preprocessing-of-next-generation-sequencing-reads</link>
	<title><![CDATA[FastProNGS: fast preprocessing of next-generation sequencing reads]]></title>
	<description><![CDATA[<p><span>FastProNGS to integrate the quality control process with automatic adapter removal. Parallel processing was implemented to speed up the process by allocating multiple threads. Compared with similar up-to-date preprocessing tools, FastProNGS is by far the fastest.&nbsp;</span></p><p>Address of the bookmark: <a href="https://github.com/Megagenomics/FastProNGS" rel="nofollow">https://github.com/Megagenomics/FastProNGS</a></p>]]></description>
	<dc:creator>Rahul Nayak</dc:creator>
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  <guid isPermaLink='true'>https://bioinformaticsonline.com/opportunity/view/19635/walk-in-interview-for-research-associate-studentship-and-traineeship-at-bif-nehu-tura-campus</guid>
  <pubDate>Thu, 18 Dec 2014 11:02:05 -0600</pubDate>
  <link></link>
  <title><![CDATA[Walk in interview for Research Associate, Studentship and Traineeship at BIF, NEHU, Tura Campus]]></title>
  <description><![CDATA[
<p>BIOINFORMATICS INFRASTRUCTURE FACILITY (BIF)<br />Department of RDAP<br />North-Eastern Hil University, Tura Campus<br />Tura-79402, Meghalaya</p>

<p>Walk in interview for Research Associate, Studentship and Traineeship at BIF</p>

<p>Applications are invited for the Post of Research Associate, Traineeship and Studentship in the DBT sponsored Bioinformatics Infrastructure Facility (BIF) at the Bioinformatics Centre, Department of RDAP, North-Eastern Hil University, Tura Campus, Tura-79402, Meghalaya. The Posts are purely temporary and terminable at any time without prior notice or assigning any reason thereof. The person engaged, shall not be entailed for any claim implicit or explicit for permanent absorption in the University.</p>

<p>Research Associate- 01</p>

<p>Essential Qualification: M.Sc. in Bioinformatics/Biotechnology from a recognized University/ institute.</p>

<p>Desirable: PhD or Pursuing PhD in the relevant subject(s) or equivalent published work in reputed peer reviewed journals or Advance PG diploma in Bioinformatics courses.</p>

<p>Duties: Creation of database, web designing, maintenance of internet, training of students in Bioinformatics, handling and knowledge of Bioinformatics software tools and technique, conducting Bioinformatics based research and other day to day laboratory work, writing report and scientific papers.</p>

<p>Pay:Rs. 2,00/- + Admissible 10% HRA per month</p>

<p>Age: Below 35 years</p>

<p>Traineeship- 02</p>

<p>Students who have completed Masters Degree in Bioinformatics/Biotechnology or any branch of Life Sciences/Agricultural Sciences/Computer Science to cary out a project work in Bioinformatics.</p>

<p>Desirable: Prior Knowledge of programming languages such as C, JAVA, MySQL is preferable.</p>

<p>Stipend: Rs. 800/- p.m. fixed. Purely temporary for a period of six months.</p>

<p>Studentship: 02</p>

<p>Students pursuing postgraduate degree in Bioinformatics/biotechnology/Agricultural Sciences or any branch of Life Science</p>

<p>Desirable: Prior knowledge of bioinformatics/ programming language is preferable.</p>

<p>Stipend: 800/- p.m. fixed. Purely temporary for a period of six months.</p>

<p>Candidates must send the detailed Biodata via mail/post and bring al the relevant documents in original and one set of attested photocopies of the same at the time of interview. No TA/DA will be paid for attending the interview and candidates have to make their own arrangements.</p>

<p>Last date for receiving application by mail or by post: 16.02.2014</p>

<p>Contact Information:<br />Dr.B.K. Mishra<br />Cordinator BIF,<br />RDAP Department, NEHU, Tura Campus<br />Phone: 91-03651-23107<br />Fax: 91-03651-23953<br />E-mail: drbkm1972@yaho.co.in, birendramishra14@gmail.com</p>

<p>Advertisement: http://www.nehu.ac.in/Advertisements/BIF_TuraAdtvPV_171214.pdf</p>
]]></description>
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	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/43273/understanding-kmer</guid>
	<pubDate>Wed, 18 Aug 2021 04:27:51 -0500</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/43273/understanding-kmer</link>
	<title><![CDATA[Understanding kmer !]]></title>
	<description><![CDATA[<p><a href="https://en.wikipedia.org/wiki/k-mer">What is a&nbsp;<em>k-mer</em>&nbsp;anyway?</a><span>&nbsp;A&nbsp;</span><em>k-mer</em><span>&nbsp;is just a sequence of&nbsp;</span><em>k</em><span>&nbsp;characters in a string (or nucleotides in a DNA sequence). Now, it is important to remember that to get&nbsp;</span><em>all k-mers</em><span>&nbsp;from a sequence you need to get the first&nbsp;</span><em>k</em><span>&nbsp;characters, then move just a single character for the start of the next&nbsp;</span><em>k-mer</em><span>&nbsp;and so on. Effectively, this will create sequences that overlap in&nbsp;</span><code>k-1</code><span>&nbsp;positions.</span></p><p>Address of the bookmark: <a href="https://bioinfologics.github.io/post/2018/09/17/k-mer-counting-part-i-introduction/" rel="nofollow">https://bioinfologics.github.io/post/2018/09/17/k-mer-counting-part-i-introduction/</a></p>]]></description>
	<dc:creator>BioStar</dc:creator>
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  <guid isPermaLink='true'>https://bioinformaticsonline.com/opportunity/view/19597/assistant-professor-at-gauhati-university-guwahati</guid>
  <pubDate>Tue, 16 Dec 2014 01:15:30 -0600</pubDate>
  <link></link>
  <title><![CDATA[Assistant Professor at GAUHATI UNIVERSITY, GUWAHATI]]></title>
  <description><![CDATA[
<p>Advt. No.T/2014/4</p>

<p>Ref. No. GU/Estt/T/308(VI)/2014/6451-61</p>

<p>Applications are invited from the Indian citizens for five (5) teaching posts of Assistant Professor (Contractual) under various departments of Gauhati University. Details of the advertisement, other terms and conditions and the application forms are available in the University website www.gauhati.ac.in</p>

<p>Asstt. Professor (Contractual)</p>

<p>    2. M.Sc. Microbiology Course in Botany</p>

<p>    3 1.M.Sc. Microbiology/M.Sc. Botany (Specialization in Microbiology)/M.Sc. Biochemistry (1 post). (Preference will be given to candidates having experience in Biochemistry).</p>

<p>    2.M.Sc. Microbiology/M.Sc. Botany (Specialization in Microbiology)/M.Sc. Biotechnology(1 post). (Preference will be given to candidates having experience in Bioinformatics).</p>

<p>    3.M.Sc. Microbiology/M.Sc. Botany (Specialization in Microbiology)/M.Sc.  Biotechnology(1 post). (Preference will be given to candidates having experience in Microbial Genetics).</p>

<p>As per UGC norms</p>

<p>Pay Band &amp; Academic Grade Pay : (Consolidated pay) : Rs. 21,600/- per month</p>

<p>Application Form : Prescribe application form may download from the G.U. website www.gauhati.ac.in</p>

<p>Last date of receipt of filled-in application is 08.01.2015.</p>

<p>Advertisement: www.gauhati.ac.in/openfile.php?file=Notice1258.pdf</p>
]]></description>
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	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/44373/mitohifi-a-python-pipeline-for-mitochondrial-genome-assembly-from-pacbio-high-fidelity-reads</guid>
	<pubDate>Tue, 05 Sep 2023 07:31:35 -0500</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/44373/mitohifi-a-python-pipeline-for-mitochondrial-genome-assembly-from-pacbio-high-fidelity-reads</link>
	<title><![CDATA[MitoHiFi: a python pipeline for mitochondrial genome assembly from PacBio high fidelity reads]]></title>
	<description><![CDATA[<p dir="auto">MitoHiFi v3.2 is a python pipeline distributed under&nbsp;<a href="https://github.com/marcelauliano/MitoHiFi/blob/master/LICENSE">MIT License</a>&nbsp;!</p>
<p dir="auto">MitoHiFi was first developed to assemble the mitogenomes for a wide range of species in the Darwin Tree of Life Project (DToL)</p>
<p dir="auto">https://bmcbioinformatics.biomedcentral.com/articles/10.1186/s12859-023-05385-y&nbsp;</p>
<p dir="auto"><a href="https://github.com/marcelauliano/MitoHiFi/blob/master/docs/dtol-logo-round-300x132.png" target="_blank"><img src="https://github.com/marcelauliano/MitoHiFi/raw/master/docs/dtol-logo-round-300x132.png" alt="" style="border: 0px; border: 0px;"></a></p><p>Address of the bookmark: <a href="https://github.com/marcelauliano/MitoHiFi" rel="nofollow">https://github.com/marcelauliano/MitoHiFi</a></p>]]></description>
	<dc:creator>Abhi</dc:creator>
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  <guid isPermaLink='true'>https://bioinformaticsonline.com/opportunity/view/19695/china-university-of-macau-phd-position-2015-in-bioinformatics-computer-science</guid>
  <pubDate>Mon, 22 Dec 2014 00:12:49 -0600</pubDate>
  <link></link>
  <title><![CDATA[China University of Macau PhD Position 2015 in Bioinformatics, Computer Science]]></title>
  <description><![CDATA[
<p>The Computational Biology and Bioinformatics Group at the University of Macau is inviting applications for PhD Position. Applicants will work on a research project focusing on the flexible receptor protein-ligand docking algorithms for computer-aided drug design.  The candidate will be working as part of a team in developing novel metaheuristic algorithms and scoring functions for large-scale, highly flexible protein-ligand docking problems. The duration of this PhD position is 2-3 years, starting in August 2015. Remuneration paid to candidate is MOP 11000-14000/month (~USD 1375-1750/month). The applications should be submitted before March 2015.</p>

<p>Study Subject(s): PhD position is award in the field of Bioinformatics/Computer Science.<br />Course Level: Position is available for pursuing PhD degree level at the University of Macau.<br />Scholarship Provider: University of Macau<br />Scholarship can be taken at: China</p>

<p>Eligibility: The ideal candidate would be a master degree holder in Bioinformatics or related disciplines with knowledge in Medical sciences or Life sciences (with GPA of at least 3.0 on a 4-point scale or equivalent) . Knowledge in programming (C and C++) and Linux scripting are necessary; experience in molecular docking, molecular dynamics simulations or molecular modeling is an advantage. The candidate should be fluent in spoken and written English; preference will be given to applicants with good publication records in relevant areas.</p>

<p>Scholarship Open for International Students: Researchers from China can apply for this PhD position.</p>

<p>Scholarship Description:</p>

<p>The Computational Biology and Bioinformatics Group at the University of Macau is looking for a motivated PhD student in Bioinformatics or Computer Science to work on a research project focusing on the flexible receptor protein-ligand docking algorithms for computer-aided drug design.  The candidate will be working as part of a team in developing novel metaheuristic algorithms and scoring functions for large-scale, highly flexible protein-ligand docking problems.</p>

<p>Number of award(s): There is only one PhD position available.</p>

<p>Duration of award(s): The duration of this PhD position is 2-3 years.</p>

<p>What does it cover? Remuneration paid to candidate is  MOP 11000-14000/month (~USD 1375-1750/month).</p>

<p>Selection Criteria: Not Known</p>

<p>Notification: Not Known</p>

<p>How to Apply: Send your current CV, your academic transcripts, a letter of motivation and research interests, two letters of recommendations from academic faculty to Dr. Shirley Siu at shirleysiu[at]umac.mo before March 2015.</p>

<p>Scholarship Application Deadline: The applications should be submitted before March 2015.</p>
]]></description>
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	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/35055/jabba-hybrid-error-correction-for-long-sequencing-reads</guid>
	<pubDate>Fri, 05 Jan 2018 03:58:14 -0600</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/35055/jabba-hybrid-error-correction-for-long-sequencing-reads</link>
	<title><![CDATA[Jabba: Hybrid Error Correction for Long Sequencing Reads]]></title>
	<description><![CDATA[<p>Jabba is a hybrid error correction tool to correct third generation (PacBio / ONT) sequencing data, using second generation (Illumina) data.</p>
<p>Input</p>
<p>Jabba takes as input a concatenated de Bruijn graph and a set of sequences:</p>
<p>the de Bruijn graph should appear in fasta format with 1 entry per node, the meta information should be in the format:<br>&gt;NODE <br>the set of sequences should be in fasta or fastq format. These sequences will be corrected (e.g. PacBio reads). The corrections will be written to a file Jabba fasta.<br>The output is a file in fasta format with corrections of the long reads, and additionally a file in the input format containing uncorrected reads.</p>
<p>https://github.com/biointec/jabba/wiki</p>
<p>https://almob.biomedcentral.com/articles/10.1186/s13015-016-0075-7</p><p>Address of the bookmark: <a href="https://github.com/biointec/jabba" rel="nofollow">https://github.com/biointec/jabba</a></p>]]></description>
	<dc:creator>Jit</dc:creator>
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  <guid isPermaLink='true'>https://bioinformaticsonline.com/opportunity/view/19811/jnu-neurosciencesystems-biologymathematical-modeling-jrf-vacancies</guid>
  <pubDate>Fri, 26 Dec 2014 11:22:20 -0600</pubDate>
  <link></link>
  <title><![CDATA[JNU Neuroscience/Systems Biology/Mathematical modeling JRF Vacancies]]></title>
  <description><![CDATA[
<p>School of Computational and Integrative Sciences<br />Jawaharlal Nehru University<br />New Delhi 110067</p>

<p>Recruitment for Project</p>

<p>Applications were invited from the citizens of India for filling up the following temporary position for the CSIR sponsored Fellowship in the School of Computational and Integrative Sciences, Jawaharlal Nehru University, New Delhi 110067. This position is temporary for a period of two and half years or tenable only for the duration of the project. The requisite qualifications &amp; experience are given below.</p>

<p>Project Title : "Understanding Complex dynamics and Information processing in Brain Networks"<br />Funding Agency : CSIR</p>

<p>Principal Investigator : Dr. R.K. Brojen Singh</p>

<p>Position : Junior Research Fellow(One post)</p>

<p>Salary : As per CSIR rules and guidelines for JRF.</p>

<p>Qualifications &amp; Experience : M.Sc. in Physics/Mathematics/Biology/B.Tech. In Eng. Physics/Comp. Sc. and desirable CSIR-UGC NET Qualified. Candidates should also have at least one years research experience after M. Sc./B.Tech. in works related to Neuroscience/Mathematical modeling.</p>

<p>Candidates possessing requisite qualifications may apply either on plain paper stating the project title along with CV and send to the following address or send as email attachment (pdf or word format) so as to reach on or before 8 January, 2014.</p>

<p>Dr. R.K. Brojen Singh<br />School of Computational and Integrative Sciences<br />Jawharlal Nehru University<br />New Delhi 110067<br />Email: brojen@jnu.ac.in, brojen@mail.jnu.ac.in</p>
]]></description>
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	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/40893/quorum-an-error-corrector-for-illumina-reads</guid>
	<pubDate>Tue, 04 Feb 2020 23:26:55 -0600</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/40893/quorum-an-error-corrector-for-illumina-reads</link>
	<title><![CDATA[QuorUM: An Error Corrector for Illumina Reads]]></title>
	<description><![CDATA[<p><span>We produce trimmed and error-corrected reads that result in assemblies with longer contigs and fewer errors. We compared QuorUM against several published error correctors and found that it is the best performer in most metrics we use. QuorUM is efficiently implemented making use of current multi-core computing architectures and it is suitable for large data sets (1 billion bases checked and corrected per day per core)</span></p><p>Address of the bookmark: <a href="http://www.genome.umd.edu/" rel="nofollow">http://www.genome.umd.edu/</a></p>]]></description>
	<dc:creator>BioStar</dc:creator>
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	<guid isPermaLink="true">https://bioinformaticsonline.com/blog/view/44848/trust-but-verify-sequencing-your-cell-lines-might-reveal-an-uninvited-guest</guid>
	<pubDate>Wed, 04 Jun 2025 00:07:57 -0500</pubDate>
	<link>https://bioinformaticsonline.com/blog/view/44848/trust-but-verify-sequencing-your-cell-lines-might-reveal-an-uninvited-guest</link>
	<title><![CDATA[Trust But Verify: Sequencing Your Cell Lines Might Reveal an Uninvited Guest]]></title>
	<description><![CDATA[<p>High-throughput sequencing has become indispensable in cell biology, enabling detailed insights into chromatin structure, gene expression, and regulatory dynamics. Yet, when faced with unexpectedly low mapping rates to the human genome, researchers often rush to troubleshoot technical parameters&mdash;sequencer quality, adapter trimming, or aligner settings.</p><p>Before you go down that path, consider this critical biological question:<br /> <strong>Are you sequencing human cells&mdash;or bacterial contamination?</strong></p><h2>The Silent Saboteur: Mycoplasma in Cell Cultures</h2><p><em>Mycoplasma</em> contamination remains one of the most widespread and underdiagnosed issues in tissue culture work. Studies suggest that <strong>15&ndash;35% of cell lines in use may be contaminated</strong>, often without visible signs. Unlike other microbial infections, <em>Mycoplasma</em> does not produce cloudiness, odor, or a change in pH. Many researchers won&rsquo;t detect it unless they specifically test for it.</p><p>The consequences, however, are profound. <em>Mycoplasma</em> can significantly alter:</p><ul>
<li>
<p>Host gene expression patterns</p>
</li>
<li>
<p>Cell proliferation rates</p>
</li>
<li>
<p>Epigenetic profiles and chromatin accessibility</p>
</li>
<li>
<p>Cytokine signaling and immune responses</p>
</li>
</ul><p>In short, it can skew your results, compromise your biological conclusions, and invalidate weeks or months of research.</p><h2>A Simple Diagnostic Step: Map Against <em>Mycoplasma</em> Genomes</h2><p>If you encounter poor alignment rates to the human genome, consider mapping your reads to a <em>Mycoplasma</em> reference genome&mdash;or better yet, use a <strong>combined human + <em>Mycoplasma</em></strong> reference. There have been cases where over half of all reads, initially assumed to be from human cells, were in fact bacterial in origin. This check is fast, easy, and could save your project.</p><h2>How Contamination Happens&mdash;and Persists</h2><p><em>Mycoplasma</em> is small (0.1&ndash;0.3 &mu;m), lacks a cell wall, and can pass through standard filters undetected. Common sources include:</p><ul>
<li>
<p>Contaminated reagents (e.g., FBS)</p>
</li>
<li>
<p>Infected cell lines obtained from other labs</p>
</li>
<li>
<p>Poor aseptic technique or shared equipment</p>
</li>
</ul><p>Once present, it spreads quickly between cultures and can persist for months, silently affecting results.</p><h2>Why Treatment Is Difficult</h2><p>While antibiotics such as Plasmocin or BM-Cyclin are sometimes used, they often offer only partial resolution and may themselves alter cell behavior. In many cases, the best course of action is to <strong>discard the contaminated culture</strong> and start with a fresh, verified stock.</p><h2>Practical Recommendations for Researchers</h2><ul>
<li>
<p><strong>Routinely test for <em>Mycoplasma</em></strong> using PCR, qPCR, or fluorescence-based assays</p>
</li>
<li>
<p><strong>Incorporate contamination screens into your sequencing QC pipeline</strong></p>
</li>
<li>
<p><strong>Use combined reference genomes</strong> when mapping ambiguous reads</p>
</li>
<li>
<p><strong>Practice strict aseptic technique</strong> and monitor all incoming cell lines</p>
</li>
<li>
<p><strong>Don&rsquo;t ignore unexplained data anomalies</strong>&mdash;they might point to contamination</p>
</li>
</ul><h2>Closing Thought: Contamination Is a Biological Variable</h2><p>It&rsquo;s easy to view poor mapping as a technical issue, but sometimes the problem lies deeper&mdash;in the biology itself. <em>Mycoplasma</em> contamination doesn&rsquo;t just interfere with sequencing; it interferes with science. As a research community, we must treat contamination not as an afterthought, but as a key variable to control.</p><p>So next time your reads won&rsquo;t align, don&rsquo;t just tune the aligner. Ask if your cells are telling the truth&mdash;or if they're hiding something.</p>]]></description>
	<dc:creator>LEGE</dc:creator>
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