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	<title><![CDATA[BOL: Related items]]></title>
	<link>https://bioinformaticsonline.com/related/28121?offset=60</link>
	<atom:link href="https://bioinformaticsonline.com/related/28121?offset=60" rel="self" type="application/rss+xml" />
	<description><![CDATA[]]></description>
	
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	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/31564/htslib</guid>
	<pubDate>Wed, 15 Mar 2017 11:38:05 -0500</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/31564/htslib</link>
	<title><![CDATA[HTSlib]]></title>
	<description><![CDATA[<p>Samtools is a suite of programs for interacting with high-throughput sequencing data. It consists of three separate repositories:</p>
<dl><dt>Samtools</dt><dd>Reading/writing/editing/indexing/viewing SAM/BAM/CRAM format</dd><dt>BCFtools</dt><dd>Reading/writing BCF2/VCF/gVCF files and calling/filtering/summarising SNP and short indel sequence variants</dd><dt>HTSlib</dt><dd>A C library for reading/writing high-throughput sequencing data</dd></dl>
<p>Samtools and BCFtools both use HTSlib internally, but these source packages contain their own copies of htslib so they can be built independently.</p><p>Address of the bookmark: <a href="http://www.htslib.org/" rel="nofollow">http://www.htslib.org/</a></p>]]></description>
	<dc:creator>Jit</dc:creator>
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<item>
  <guid isPermaLink='true'>https://bioinformaticsonline.com/opportunity/view/915/researcher-in-computer-sciencebiology</guid>
  <pubDate>Mon, 15 Jul 2013 18:38:40 -0500</pubDate>
  <link></link>
  <title><![CDATA[Researcher in computer science/biology]]></title>
  <description><![CDATA[
<p>Researcher in Computer Science at the Computational Biology Unit - temporary employment</p>

<p>The Department of Informatics is a vacant position as a researcher in computer science, related to Computational Biology Unit (CBU), for 3 years.<br /> <br />The position is part of CBU Service Group and will focus on bioinformatic analysis project and especially the analysis of high-throughput data, including NGS (sequencing), and proteomics data.<br /> <br />The successful candidate will be part of the Norwegian bioinformatics platform's national helpdesk within the project ELIXIR.NO<br /> <br />Applicants must hold a PhD in a relevant subject such as computer science, mathematics, molecular biology and also possess expertise and experience in bioinformatics statistics and analysis of data from high-throughput molecular experiment.<br /> <br />Basic programming or scripting skills are required. Experience in Python, R, Perl, Linux-based operating systems and moreover knowledge of databases and web programming will be a strength for applicants.<br /> <br />We expect enthusiasm and independence and moreover the ability to work in an interdisciplinary team environment.<br /> <br />Good knowledge of English is required.<br /> <br />Salaries start at level 57 (code 1109/LR 24.1) by appointment. Further promotion occurs after<br />service seniority in the position (at grade 57-65). Of particularly highly qualified applicants may be considered a higher salary.<br /> <br />Further information about the position is available from the chair of the CBU, <br />Professor Inge Jonassen, e-mail: Inge.Jonassen @ ii.uib.no<br /> <br />The successful applicant must comply with the guidelines that apply at any given time the position.<br /> <br />State employment shall as far as possible reflect the diversity of the population. It is therefore an objective to achieve a balanced age and sex composition and the recruitment of persons with immigrant backgrounds. Persons with immigrant background are requested to apply for the position.<br /> <br />Women are particularly encouraged to apply. If the experts find that several applicants have approximately equivalent qualifications, the rules on equal in the Personnel Regulations for Academic Positions will be applied.<br /> <br />University of Bergen applies the principles of public openness when recruiting staff to scientific positions.<br /> <br />Information about the applicant may be made public even though the applicant has requested not to be named in the list of applicants. If the request does not host admitted to the result, the applicant shall be notified of this.<br /> <br />Send application, CV, certificates, diplomas, undergraduate work and a list of publications (list of publications) online by clicking on https://www.jobbnorge.no/jobbsoknet/login.aspx?returnurl=/jobbsoknet/jobapplication.aspx?jobid=95196<br /> <br />You need to upload certified translations into English or a Scandinavian language of appendices, such as diplomas and transcripts.<br /> <br />Applications sent by email to individuals at the institute will not be considered.<br /> <br />Deadline: 9 August 2013</p>
]]></description>
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  <guid isPermaLink='true'>https://bioinformaticsonline.com/opportunity/view/2054/postdoc-positions-mammalian-transcriptome-evolution-at-sib</guid>
  <pubDate>Mon, 12 Aug 2013 19:58:33 -0500</pubDate>
  <link></link>
  <title><![CDATA[Postdoc Positions - Mammalian Transcriptome Evolution at SIB]]></title>
  <description><![CDATA[
<p>BIOINFORMATICS POSTDOC IN FUNCTIONAL EVOLUTIONARY GENOMICS</p>

<p>Center for Integrative Genomics, University of Lausanne, Switzerland</p>

<p>Two postdoctoral positions (2 years with possible extensions up to 5 years) are available immediately in the evolutionary genomics group of Henrik Kaessmann.</p>

<p>We are seeking highly qualified and enthusiastic applicants with strong skills in computational biology/bioinformatics, preferably also with experience in data mining and comparative or evolutionary genome analysis.</p>

<p>We have been interested in a range of topics related to the functional evolution of genomes from primates (e.g., the emergence of new genes and their functions) and other mammals (e.g., the origin and evolution of mammalian sex chromosomes). In the framework of a recently launched series of projects, a large amount of transcriptome and genome (e.g., epigenome) data are being produced by the wet lab unit of the group using next generation sequencing technologies for a unique collection of tissues from representative mammals and outgroup species (e.g., birds). Topics of current projects based on these data include the origins and/or evolution of protein-coding genes, alternative splicing, microRNAs, long noncoding RNAs, and dosage compensation.</p>

<p>The postdoctoral fellow will perform integrated evolutionary/bioinformatics analyses based on data produced in the lab and available genomic data. The specific project will be developed together with the candidate.</p>

<p>The language of the institute is English, and its members form an international group that is rapidly expanding. The institute is located in Lausanne, a beautiful city at Lake Geneva.</p>

<p>For more information on the group and our institute more generally, please refer to our website: http://www.unil.ch/cig/page7858_en.html</p>

<p>Please submit a CV, statement of research interest, and names of three references to: Henrik Kaessmann (Henrik.Kaessmann@unil.ch).</p>

<p>Webpage : http://www.unil.ch/cig/page7858.html</p>
]]></description>
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	<guid isPermaLink="true">https://bioinformaticsonline.com/pages/view/40226/bioinformatics-training-courses-at-rasa-lsi</guid>
	<pubDate>Wed, 06 Nov 2019 00:30:51 -0600</pubDate>
	<link>https://bioinformaticsonline.com/pages/view/40226/bioinformatics-training-courses-at-rasa-lsi</link>
	<title><![CDATA[Bioinformatics Training Courses At RASA LSI]]></title>
	<description><![CDATA[<p>RASA conducts comprehensive Life Science skill development training courses in Pune, India for working professionals, researchers, students and job-seeker. The trainings are crafted meticulously, covering different modules of courses such as Bioinformatics course, In silico Drug Discovery course, Next Generation Sequence data analysis course, Molecular Biology &amp; Life&nbsp;science software development course wherein you learn from industry leaders&nbsp;how to apply these skills in life science &amp; have a command over software developing process &nbsp;by using various methodologies. We conduct in-class training and instructor-led live online classes worldwide, along with corporate and skill development training worldwide.</p><p>Workshops are conducted in regular intervals on Drug Designing, Protein Modeling and Simulation, Chemoinformatics, Bioinformatics etc.The workshops are highly beneficial for working professionals, students, researcher for enhancements of the skills in short duration.</p>]]></description>
	<dc:creator>RASA Life Sciences</dc:creator>
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	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/41991/sequence-ontology-bioinformatics-analysis-soba-tool-to-provide-a-simple-statistical-and-graphical-summary-of-an-annotated-genome</guid>
	<pubDate>Wed, 22 Jul 2020 10:11:13 -0500</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/41991/sequence-ontology-bioinformatics-analysis-soba-tool-to-provide-a-simple-statistical-and-graphical-summary-of-an-annotated-genome</link>
	<title><![CDATA[Sequence Ontology Bioinformatics Analysis (SOBA) tool to provide a simple statistical and graphical summary of an annotated genome]]></title>
	<description><![CDATA[<p><span>We have developed the Sequence Ontology Bioinformatics Analysis (SOBA) tool to provide a simple statistical and graphical summary of an annotated genome. We envisage its use during annotation jamborees, genome comparison and for use by developers for rapid feedback during annotation software development and testing. SOBA also provides annotation consistency feedback to ensure correct use of terminology within annotations, and guides users to add new terms to the Sequence Ontology when required. SOBA is available at http://www.sequenceontology.org/cgi-bin/soba.cgi.</span></p>
<p><span>More at <a href="https://pubmed.ncbi.nlm.nih.gov/20494974/">https://pubmed.ncbi.nlm.nih.gov/20494974/</a></span></p><p>Address of the bookmark: <a href="http://www.sequenceontology.org/cgi-bin/soba.cgi" rel="nofollow">http://www.sequenceontology.org/cgi-bin/soba.cgi</a></p>]]></description>
	<dc:creator>BioStar</dc:creator>
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  <guid isPermaLink='true'>https://bioinformaticsonline.com/opportunity/view/43292/bioinformatics-scientist-production-bioinformatics-south-san-francisco-ca</guid>
  <pubDate>Thu, 19 Aug 2021 08:45:24 -0500</pubDate>
  <link></link>
  <title><![CDATA[Bioinformatics Scientist, Production Bioinformatics @ South San Francisco, CA]]></title>
  <description><![CDATA[
<p>wist is looking for a Bioinformatics Scientist to join our Production Bioinformatics Team. You will work alongside research scientists, software engineers and data scientists to further deliver on our mission to expand access to best-in-class synthetic biology and next-generation sequencing applications. You will be developing and engineering tools to better evaluate and build hardened, production quality pipelines, optimize data quality, and automate lab and bioinformatics processes. Our ideal candidate is an organized problem solver with a background in developing and building novel production-quality bioinformatics tools and packages. Equally excellent communication skills and a proven ability to work independently are required.</p>

<p>More at https://boards.greenhouse.io/twistbioscience/jobs/3135495?gh_src=9ecc0b941us</p>
]]></description>
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	<guid isPermaLink="true">https://bioinformaticsonline.com/blog/view/44720/a-beginners-guide-to-using-kraken-for-taxonomic-classification</guid>
	<pubDate>Fri, 13 Dec 2024 11:29:03 -0600</pubDate>
	<link>https://bioinformaticsonline.com/blog/view/44720/a-beginners-guide-to-using-kraken-for-taxonomic-classification</link>
	<title><![CDATA[A Beginner&#039;s Guide to Using Kraken for Taxonomic Classification]]></title>
	<description><![CDATA[<div>Kraken is a popular bioinformatics tool designed for fast and accurate taxonomic classification of metagenomic sequences. Its efficiency and precision make it a go-to resource for analyzing microbial communities, including bacteria, viruses, archaea, and fungi. Whether you're new to bioinformatics or experienced in the field, Kraken is an indispensable tool for taxonomic analysis.</div><div><div><div><div dir="auto"><div><div><p>In this blog, we&rsquo;ll walk through the basics of Kraken, from installation to running an analysis, and highlight its key features and applications.</p><h4><strong>What is Kraken?</strong></h4><p>Kraken is a sequence classification tool that assigns taxonomic labels to DNA sequences using exact k-mer matching. It uses a reference database of genomes, dividing sequences into k-mers and identifying matches in a computationally efficient way.</p><h4><strong>Key Features of Kraken</strong></h4><ul>
<li><strong>Speed</strong>: Kraken processes data much faster than alignment-based methods.</li>
<li><strong>Accuracy</strong>: It uses a precise k-mer matching algorithm for high-resolution taxonomic assignments.</li>
<li><strong>Scalability</strong>: It can handle large metagenomic datasets.</li>
<li><strong>Custom Databases</strong>: You can build and use custom databases tailored to your research needs.</li>
</ul><h4><strong>Installing Kraken</strong></h4><ol>
<li>
<p><strong>System Requirements</strong></p>
<ul>
<li>A Unix-based operating system (Linux/macOS).</li>
<li>Sufficient computational resources for database building (RAM and disk space).</li>
</ul>
</li>
<li>
<p><strong>Installation Steps</strong></p>
<ul>
<li>Clone the Kraken repository from GitHub:
<div>
<div>&nbsp;</div>
<div dir="ltr"><code>git <span style="font-size: 12.8px; font-weight: normal;">clone</span> https://github.com/DerrickWood/kraken.git <span style="font-size: 12.8px; font-weight: normal;">cd</span> kraken </code></div>
</div>
</li>
<li>Compile the Kraken binaries:
<div>
<div>&nbsp;</div>
<div dir="ltr"><code>make </code></div>
</div>
</li>
<li>Add Kraken to your PATH for easy access:
<div>
<div>&nbsp;</div>
<div dir="ltr"><code><span style="font-size: 12.8px; font-weight: normal;">export</span> PATH=<span style="font-size: 12.8px; font-weight: normal;">$PATH</span>:/path/to/kraken </code></div>
</div>
</li>
</ul>
</li>
</ol><h4><strong>Preparing a Database</strong></h4><p>Kraken requires a database of reference genomes. You can use a pre-built database or create a custom one.</p><ol>
<li>
<p><strong>Downloading a Pre-built Database</strong><br />Kraken offers pre-built databases, such as the <em>MiniKraken</em> database, which is lightweight and suitable for smaller datasets. Download it using:</p>
<div>
<div dir="ltr"><code>kraken-build --download-library minikraken </code></div>
</div>
</li>
<li>
<p><strong>Building a Custom Database</strong><br />To include specific genomes, download FASTA files and build the database:</p>
<div>
<div dir="ltr"><code>kraken-build --download-library bacteria --threads 4 --db my_database kraken-build --build --db my_database </code></div>
</div>
<p>This process may take considerable time and resources, depending on the size of the database.</p>
</li>
</ol><h4><strong>Running Kraken</strong></h4><p>Once the database is ready, you can classify sequences.</p><ol>
<li>
<p><strong>Basic Usage</strong><br />Use the following command to classify sequences:</p>
<div>
<div dir="ltr"><code>kraken --db my_database --threads 4 --fastq-input input_sequences.fastq --output kraken_output.txt </code></div>
</div>
<p>Key options:</p>
<ul>
<li><code>--db</code>: Specifies the database.</li>
<li><code>--threads</code>: Number of threads for parallel processing.</li>
<li><code>--fastq-input</code>: Indicates input file format (FASTQ/FASTA).</li>
</ul>
</li>
<li>
<p><strong>Interpreting Results</strong><br />Kraken generates an output file with columns for sequence IDs, taxonomic classifications, and the confidence score.</p>
</li>
</ol><h4><strong>Visualizing Kraken Results</strong></h4><p>Kraken results can be visualized using tools like <strong>Krona</strong> or converted to human-readable reports using <code>kraken-report</code>.</p><ol>
<li>
<p><strong>Generate a Report</strong></p>
<div>
<div dir="ltr"><code>kraken-report --db my_database kraken_output.txt &gt; kraken_report.txt </code></div>
</div>
</li>
<li>
<p><strong>Krona Visualization</strong><br />Install Krona and convert Kraken output for visualization:</p>
<div>
<div dir="ltr"><code>cut -f2,3 kraken_output.txt | ktImportTaxonomy -o krona_output.html </code></div>
</div>
<p>Open the HTML file in your browser to interactively explore the taxonomic classifications.</p>
</li>
</ol><h4><strong>Advanced Usage</strong></h4><ol>
<li>
<p><strong>Confidence Thresholds</strong><br />Adjust the confidence threshold for classification using the <code>--confidence</code> option. Higher values reduce false positives but may miss some true positives:</p>
<div>
<div dir="ltr"><code>kraken --db my_database --confidence 0.1 --fastq-input input.fastq </code></div>
</div>
</li>
<li>
<p><strong>Paired-End Reads</strong><br />For paired-end sequencing data, use:</p>
<div>
<div dir="ltr"><code>kraken --db my_database --paired reads_1.fastq reads_2.fastq </code></div>
</div>
</li>
<li>
<p><strong>Customizing K-mers</strong><br />Kraken allows you to set custom k-mer lengths during database building for specific applications.</p>
</li>
</ol><h4><strong>Applications of Kraken</strong></h4><ul>
<li><strong>Microbial Ecology</strong>: Characterizing microbial communities in soil, water, and the human microbiome.</li>
<li><strong>Pathogen Detection</strong>: Identifying pathogens in clinical samples.</li>
<li><strong>Fungal Research</strong>: Analyzing fungal diversity in metagenomic datasets.</li>
<li><strong>Environmental Monitoring</strong>: Tracking microbial populations in diverse habitats.</li>
</ul><h4><strong>Conclusion</strong></h4><p>Kraken is a versatile and efficient tool for taxonomic classification in metagenomics. Its speed, accuracy, and flexibility make it a favorite among bioinformaticians. By following this guide, you can set up and use Kraken to unlock insights into microbial and fungal communities, paving the way for discoveries in ecology, medicine, and biotechnology.</p></div></div></div></div></div></div>]]></description>
	<dc:creator>Neel</dc:creator>
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  <guid isPermaLink='true'>https://bioinformaticsonline.com/researchlabs/view/7088/gabi</guid>
  <pubDate>Fri, 06 Dec 2013 16:43:01 -0600</pubDate>
  <link></link>
  <title><![CDATA[GABi]]></title>
  <description><![CDATA[
<p>GABi Research<br />The major researching fields defined as the GABi scope are described next:<br />    Sequence Analysis<br />    Protein Structure Prediction<br />    Comparative Genomics<br />    Functional Analysis of Residues on Protein Families<br />    Gene/Protein Networks<br />    Genome structure &amp; base composition<br />    Highthroughput data analysis from NGS</p>

<p>Lab Page http://gabi.cidbio.org/index/</p>
]]></description>
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  <guid isPermaLink='true'>https://bioinformaticsonline.com/opportunity/view/10881/special-project-scientist-%E2%80%93-sorghum-genomics</guid>
  <pubDate>Tue, 20 May 2014 00:34:39 -0500</pubDate>
  <link></link>
  <title><![CDATA[Special Project Scientist – Sorghum Genomics]]></title>
  <description><![CDATA[
<p>ICRISAT is seeking applications from Indian Nationals for a Special Project Scientist to work on a sorghum genomics activities related to sequencing/re-sequencing projects utilizing New Generation Sequencing platforms.</p>

<p>The Job detail</p>

<p>    Advancing the SNP-discovery and polymorphism assessment work across several germplasm panels representing global genetic diversity<br />    Population genetic and genomic analyses, testing the hypothesis related to adaptation in multiple geographic regions<br />    Develop SNP assays from large scale GBS and other re-sequencing data for several target traits utilizing available phenotyping data<br />    Combined analyses of genotypic and phenotypic data for discovery of marker-trait associations, and conducting GWAS<br />    Processing, analyzing, and archiving large-scale genomic data sets, assessing data quality, conducting analyses, interpreting findings, and communicating findings to others including preparation of reports, presentations, posters and journal articles<br />    Providing support to MSc and PhD students on topic related to its major core of research<br />    Any other work assigned by the supervisor</p>

<p>The Person:</p>

<p>    PhD in bioinformatics, genetics, computational biology preferably with 1 to 2 years of experience;<br />    familiar with standard bioinformatics tools and scripting languages and emerging and evolving software platforms relevant to bioinformatics and computational biology;<br />    ability to create new analytical pipelines; experience with handling large data sets;<br />    ability to program in at least two of the following: C++, PERL, Python, R, Java.<br />    will use next-generation sequencing technologies to generate marker data for genetic mapping and transcriptome data for expression QTL mapping, and will be responsible for data generation as well as data analysis.</p>

<p>Period and Remuneration: The assignment is for a period of two years, and can be extended for another year depending on performance. ICRISAT pays a very attractive all inclusive lump sum assignment fee payable in Indian Rupees.</p>

<p>How to Apply: Please send your application by email to icrisatjobs@cgiar.org, stating the job title (Special project Scientist-Sorghum Genomics) clearly in the subject column, addressed to the Director, Human Resources and Operations, ICRISAT, Patancheru, Andhra Pradesh 502 324, India, latest by 10 June 2014. The application should include an up-to-date Curriculum Vitae, a short statement of competencies and experience for the position, and the names and addresses (including phone/e-mail) of three referees. Only short-listed candidates will be contacted.</p>

<p>More at: http://www.icrisat.org/careers/Special-Project-Scientist-Sorghum-Genomics.htm</p>
]]></description>
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  <guid isPermaLink='true'>https://bioinformaticsonline.com/opportunity/view/10841/ra-at-iisr-kozhikode</guid>
  <pubDate>Thu, 15 May 2014 10:08:09 -0500</pubDate>
  <link></link>
  <title><![CDATA[RA at IISR Kozhikode]]></title>
  <description><![CDATA[
<p>INDIAN INSTITUTE OF SPICES RESEARCH<br />(Indian Council of Agricultural Research)<br />Marikunnu P.O., Kozhikode – 673 012, Kerala</p>

<p>Walk- in- Test cum Interview (based on test) for the selection of Research Associate</p>

<p>under the scheme “Distributed Information Sub Centre –DISC” &amp; Research Assistant under scheme “Phytophthora, Fusarium and Ralstonia diseases of Horticultural and Field Crops” will be held at this Institute as per details indicated below.</p>

<p>WALK -IN- TEST CUM INTERVIEW</p>

<p>Name of the post : Research Associate</p>

<p>Date of Interview : 21-05-2014 at 10.00 AM</p>

<p>No. of posts : One</p>

<p>Qualifications : a)Essential</p>

<p>Ph.D Degree in Bioinformatics OR :  Masters degree in Bioinformatics with a minimum of<br />60% marks or equivalent OGPA with at least two years research experience as evidenced from fellowship/ associateship/training/published papers etc.</p>

<p>b)Desirable: Experience in NGS data analysis.</p>

<p>Emoluments : Rs. 23,000/- per month + HRA (Masters Degree Holders)</p>

<p>Rs. 24,000/- per month + HRA (Ph.D Degree Holders)</p>

<p>Upper age limit : 40 years for Men &amp; 45 years for Women as on date of Interview (Upper Age limits are relaxable for SC, ST and OBC candidates as per Govt. of India norms (at present 5 years for SC/ST and 3 years for OBC)</p>

<p>Duration of Project : Till 31-03-2017.</p>

<p>Title of Assigment : Research Assistant (on contract basis)</p>

<p>No. of vacancy : One</p>

<p>Qualification : Essential : Post Graduation in Bioinformatics and  Minimum one year experience in NGS data analysis</p>

<p>Desirable : Experience in Perl/Python/R</p>

<p>Remuneration : Rs. 20,000/- per month (consolidated)</p>

<p>Scope of work :</p>

<p>1. Analysis of different file formats and their conversions.</p>

<p>2. Assessing the quality of data and filtering of raw reads.<br />3. Assembling the raw reads-de novo as well as reference  mapping.<br />4. Compression of aligned reads using Jam tools<br />5. RNA-seq. Analysis<br />6. Differential expression testing involving Normalization,  Statistical testing, heat map generation &amp; hierarchical  clustering<br />7. Annotating the assembled genome and geneet testing  and their validation<br />8. Metabolic pathway analysis<br />9. Comparative genomics<br />10. Setting up of genome browsers.</p>

<p>Period of Assigment : Initially for six months.</p>

<p>Date &amp; Venue of Interview : 21-05-2014 at IISR, Kozhikode at 10.00 AM</p>

<p>More at http://www.spices.res.in/pdf/disc-advtmnt.pdf</p>
]]></description>
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