Calculate how much sequencing you need to hit a target depth of coverage (or vice versa).

Instructions: set the read length/configuration and genome size, then select what you want to calculate.

Written by Stephen Turner, based on the Lander-Waterman formula, inspired by a similar calculator written by James Hadfield. Coverage is calculated as C=LN/G and reads as N=CG/L where C = Coverage (X),L = Read length (bp), G = Haploid genome size (bp), and N = Number of reads. Source code on GitHub.

Address of the bookmark: http://apps.bioconnector.virginia.edu/covcalc/

Karnal -132001 (Haryana)

A walk-in-Interview is proposed to be held at National Bureau of Animal Genetic Resources, Karnal (Haryana)-132001 at 10:30 AM on 05.09.2016 for the selection of Three Research Associate & One Young Professional - II as per details given below:

Name of the Scheme / Project: Center for Agricultural Bioinformatics. The post duration is Upto 31.032017 or earlier & Co-terminus with the project.

Research Associate (Three posts)

Date & Time of Interview: 10.30 A.M. on 05.09.2016

Essential Qualifications: PhD degree in any one of discipline/Subject Biotechnology/ Animal Genetics and Breeding/ Biochemistry/ Bioinformatics/Molecular Genetics OR Master’s degree in any one of above mentioned discipline/Subject with 4 years/5 years of Bachelor’s degree having 1st division or 60% marks or equivalent overall grade point average, with at least two years of research experience as evidenced from Fellowship/Associateship

Desirable Qualifications: Experience in Database/Next Generation Sequencing Data analysis for 02 RA posts or working experience in molecular biology, gene expression data analysis, SNP genotyping and sequence data analysis, functional gene characterization for 01 RA post.

Young Professionals II One position

Date & Time of Interview: 10.30 A.M. on 05.09.2016

Essential: B. Tech or M.Tech. in Bioinformatics / Computer Science / Computer Application.

Desirable: Experience in Linux, MySQL, Java, C++/ PHP/ PERL R based data analysis and application development in Bioinformatics.

More Info : http://14.139.252.116/ADvertisementforCabinScheme.pdf

Candidates are required to appear for an interview, with all the necessary certificates in original along with a set of attested copies in the office of the Principal, AU College of Science & Technology, Andhra University, Visakhapatnam. Applications may be sent by Email to andhrauniv.btisnet@nic.in / katruumadevi@gmail.com.

[uzi@quince-srv2 ~/]$ cp -r /home/opt/MScBioinformatics/linuxTutorial .
[uzi@quince-srv2 ~/]$ cd linuxTutorial
[uzi@quince-srv2 ~/linuxTutorial]$

I have deliberately chosen Awk in the exercises as it is a language in itself and is used more often to manipulate NGS data as compared to the other command line tools such as grep, sed, perl etc. Furthermore, having a command on awk will make it easier to understand advanced tutorials such as Illumina Amplicons Processing Workflow.

In Linux, we use a shell that is a program that takes your commands from the keyboard and gives them to the operating system. Most Linux systems utilize Bourne Again SHell (bash), but there are several additional shell programs on a typical Linux system such as ksh, tcsh, and zsh. To see which shell you are using, type

[uzi@quince-srv2 ~/linuxTutorial]$ echo $SHELL

/bin/bash

Address of the bookmark: http://userweb.eng.gla.ac.uk/umer.ijaz/bioinformatics/linux.html

This utility makes it easy to identify what are the properties of a read based on its SAM flag value, or conversely, to find what the SAM Flag value would be for a given combination of properties.

To decode a given SAM flag value, just enter the number in the field below. The encoded properties will be listed under Summary below, to the right.

Address of the bookmark: https://broadinstitute.github.io/picard/explain-flags.html

By default, Kaiju uses either the available complete genomes from NCBI RefSeq or the microbial subset of the non-redundant protein database nr used by NCBI BLAST, optionally also including fungi and microbial eukaryotes.

Kaiju translates reads into amino acid sequences, which are then searched in the database using a modified backward search on a memory-efficient implementation of the Burrows-Wheeler transform, which finds maximum exact matches (MEMs), optionally allowing mismatches in the protein alignment. The search can process up to millions of reads per minute using, for example, only 10 GB RAM with a protein database comprising 4821 microbial genomes. Kaiju can also be used for querying any other protein database without taxonomic classification, using either protein or nucleotide queries.

Kaiju is described in Menzel, P. et al. (2016) Fast and sensitive taxonomic classification for metagenomics with Kaiju. Nat. Commun. 7:11257 (open access).

Address of the bookmark: http://kaiju.binf.ku.dk/

Source: http://www.nipgr.res.in/careers/vacancies_latest.php#
Form at http://www.nipgr.res.in/files/careers/format_RA_JRF_LA.doc

WiseScaffolder is a stand-alone semi-automatic application for genome scaffolding of pre-assembled contigs using mate-pair data. It also produces editable scaffold maps, allowing either to build gapped scaffolds or usable as a common thread for the manual improvement of scaffolds.

Description 

WiseScaffolder includes 4 subcommands: dumpconfig generates a configuration file that notably specifies the average insert size of the mate-pair library preprocess allows the detection and correction of chimerae, the estimation of contigs copy number and produces valuable outputs for the manual improvement of scaffolds scaffold constitutes the central scaffold-builder and comprises two modules:

i) the interative_scaffold_extender, which works with big, unambiguous contigs, or when they run out, single copy contigs, and

ii) the small_contig_inserter, which inserts the small contigs within scaffolds buildfasta converts the scaffold(s) map(s) into Fasta sequences.

Address of the bookmark: http://abims.sb-roscoff.fr/wisescaffolder

Research Associate /01 Post

Essential: Ph.D. in Bioinformatics or 03 years research experience after Post Graduation in Bioinformatics with at least one research paper in Science Citation Indexed (SCI) journals.

Desirable: The candidate should have at least 1st Division during Graduation and Post Graduation. Experience in assembly/ analysis/ annotation of genomic/transcriptomic data generated on next generation sequencing platforms and working knowledge on different genomic softwares. Publications in Relevant Field.

Pay Scale : Rs. 36,000/- +20% HRA

Age: 40 years for male and 45 years for female candidates, as on the date of interview

Junior Research Fellow/ 01

Essential: Master Degree in Biotechnology/Life Science with Specialization in Molecular Biology with NET qualification.

Desirable: Research Experience in Molecular Biology. 1st Division during Graduation as well as Post Graduation. Publications in Relevant Field.

Pay Scale: Rs. 25,000/-+ 20% HRA for 1st and 2nd year and Rs. 28,000/-+ 20% HRA for 3rd year

Age: 35 years for male and 40 years for female candidates, as on the date of interview.
How to apply
A walk-in-interview will be held on 26.07.2016 at 10:00 hrs. at ICAR-National Bureau of Fish Genetic Resources, Lucknow.

More at http://www.nbfgr.res.in/Recruitments.aspx

DBT Invites online applications from the bioinformatics students and requisitions from biotech/bioinformatics companies.

Biotech Industry :

Biotech/Bioinformatics companies interested to provide hands on industrial training to the students of Bioinformatics under BIITP may apply online. The companies would have no obligation towards any payments to trainees. The companies would be paid bench fee to cover expenses towards training. Trainees would be provided to companies subject to availability.

Attn: Bioinformatics Students

Bioinformatics students interested in training in biotech / bioinformatics companies may apply online. Stipend of Rs. 10,000/- per month will be paid to candidates placed for training. The candidates will be selected for training through an interview.

Eligiblity :

a) B.E /B.Tech./M.Sc./M.Tech./Advanced Post Graduate Diploma in Bioinformatics from an Indian recognized university with minimum 55% marks or equivalent grade at highest degree/diploma completed in the year 2015 or 2016 are only eligible to apply.

b) The Advanced Post Graduate diploma should be of at least one year duration after graduation.

c)  Students whose result of last semester/final year is not declared can also apply mentioning their marks upto the semester/year upto which result declared. The final result with original mark sheet(s) of all the semesters/years will have to be produced at the time of interview.

Application Procedure :

The online application form is available below :

Application Form For Students (New User)

Requisition form for companies (New User)

Already registered User Click Here

The following documents are to be sent to Mr. Manoj Gupta, Manager, Biotech Consortium India Limited, 5th floor, Anuvrat Bhawan, 210, Deen Dayal UpadhyayaMarg, New Delhi-110002.

More at http://www.bcil.nic.in/biitp2016-17/index.asp