BOL: Related items

Meraculous: De Novo Genome Assembly with Short Paired-End Reads

Jit — Tue, 07 Nov 2017 04:36:10 -0600

We describe a new algorithm, meraculous, for whole genome assembly of deep paired-end short reads, and apply it to the assembly of a dataset of paired 75-bp Illumina reads derived from the 15.4 megabase genome of the haploid yeast Pichia stipitis. More than 95% of the genome is recovered, with no errors; half the assembled sequence is in contigs longer than 101 kilobases and in scaffolds longer than 269 kilobases. Incorporating fosmid ends recovers entire chromosomes. Meraculous relies on an efficient and conservative traversal of the subgraph of the k-mer (deBruijn) graph of oligonucleotides with unique high quality extensions in the dataset, avoiding an explicit error correction step as used in other short-read assemblers. A novel memory-efficient hashing scheme is introduced. The resulting contigs are ordered and oriented using paired reads separated by ∼280 bp or ∼3.2 kbp, and many gaps between contigs can be closed using paired-end placements. Practical issues with the dataset are described, and prospects for assembling larger genomes are discussed.

Address of the bookmark: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3158087/

RA Bioinformatics at Ch. Charan Singh University, Meerut

Wed, 11 Mar 2015 09:07:07 -0500

Ch. Charan Singh University, Meerut

http://molbiolabccsumrt.webs.com/

Applications are invited for one post of RA in a DBT funded research project “Creation of Bioinformatics Infrastructure Facility (BIF) for the promotion of Biology Teaching through Bioinformatics (BTBI) Scheme of BTISet”.

Candidate should have a Ph.D. degree in Bioinformatics/Biotechnology/Genetics and Plant Breeding with adequate experience in the area of Bioinformatics. If a suitable candidate for the post of RA is not available, a JRF/SRF may be appointed.

Candidate for the post of JRF/SRF should have Master’s degree in relevant subject with adequate experience in the area of Bioinformatics and should be NET/DBT-BINC qualified

Interested candidates may send their bio-data to Prof. H. S. Balyan (hsbalyan@gmail.com) (in exceptional case, bio-data may also be submitted at the time of interview) and attend the interview on Monday, March 30, 2015 at 11:00 AM in the Department of Genetics & Plant Breeding, Ch. Charan Singh University, Meerut. Candidates shall bring their original documents at the time of interview for verification. No interview letters will be issued and no TA/DA will be paid.

funannotate: Eukaryotic Genome Annotation Pipeline

Jit — Wed, 19 Sep 2018 07:47:22 -0500

Funannotate is a genome prediction, annotation, and comparison software package. It was originally written to annotate fungal genomes (small eukaryotes ~ 30 Mb genomes), but has evolved over time to accomodate larger genomes. The impetus for this software package was to be able to accurately and easily annotate a genome for submission to NCBI GenBank. Existing tools (such as Maker) require significant manually editing to comply with GenBank submission rules, thus funannotate is aimed at simplifying the genome submission process.

Address of the bookmark: https://github.com/nextgenusfs/funannotate

Coding Ground

Jitendra Narayan — Tue, 17 Mar 2015 00:47:20 -0500

Online coding group for most of the programming languages.

Code in almost all popular languages using Coding Ground. Edit, compile, execute and share your projects, 100% cloud.

http://www.tutorialspoint.com/codingground.htm

Address of the bookmark: http://www.tutorialspoint.com/codingground.htm

Earth BioGenome Project

Jit — Wed, 25 Apr 2018 07:48:56 -0500

The central goal of the Earth BioGenome Project is to understand the evolution and organization of life on our planet by sequencing and functionally annotating the genomes of 1.5 million known species of eukaryotes, a massive group that includes plants, animals, fungi and other organisms whose cells have a nucleus that houses their chromosomal DNA. To date, the genomes of less than 0.2 percent of eukaryotic species have been sequenced.

More at https://www.ucdavis.edu/news/earth-biogenome-project-aims-sequence-dna-all-complex-life

Bioinformatics Project Assistant at NITW

Wed, 01 Apr 2015 01:43:27 -0500

NATIONAL INSTITUTE OF TECHNOLOGY WARANGAL – 506 004

DEPARTMENT OF BIOTECHNOLOGY

No. NITW/Bio/ Date: 30th March 2015

ADVERTISEMENT FOR PROJECT FELLOW (Research Staff)

Applications are invited for the post of Project Fellow (Research Staff) for the project entitled ‘Metagenome derived nitroreductases for degradation of nitro compounds’ sponsored by the Department of Science and Technology (DST-INSPIRE), Govt. of India.

Position: Project Fellow (1 No.)

Project Duration: 5 years

Note: The post is purely on temporary basis for a period one year and may be extendable till the end of the project based on the progress of the candidate.

Emoluments: Rs. 14,000/- per month (Consolidated) for first two years and Rs. 16,000/- per month (Consolidated) for next three years.

Essential Qualifications:

i) First class in M.Tech/M.S (Biotechnology/Industrial Biotechnology/Bioinformatics) or equivalent. Or

ii) First class in M.Sc. (Biotechnology/Bioinformatics/Biochemistry/ Microbiology/Molecular biology).

Desirable Qualification: Preference will be given to candidates who have cleared NET/GATE or having prior work experience in Molecular biology/Bioinformatics sequence analysis.

Interested candidates may submit their application in plain paper along with Curriculum Vitae and photocopies of certificates in support of educational/professional qualifications. Application should be sent in a closed cover with a superscription on the cover “Application for the post of project fellow (DST-INSPIRE)” on or before 20.04.2015 (Monday) via Post to the Principal Investigator. Eligible candidates will be intimated through e-mail and called for interview at the Department of Biotechnology, NIT, Warangal. No TA/DA will be paid for attending the interview. Dr. K. Divakar (Principal Investigator) DST-INSPIRE Faculty Department of Biotechnology National Institute of Technology Warangal – 506 004. Telangana, India. E-mail: divakar@nitw.ac.in; kdivak@gmail.com

Advertisement: http://www.nitw.ac.in/nitw/announcements/2015/Notification_Project_Fellow_DST_INSPIRE_Biotechnology_NITW.pdf

Bioinformatics RA at IVRI

Tue, 07 Apr 2015 03:10:53 -0500

A walk in interview is scheduled in the seminar hall of Veterinary Biotechnology Division of the institute on 9.04.15 at 10.30 am for the engagement of one Research associate (RA) in ICAR funded time bound project entitled “Centre for Agricultural bioinformatics (CABin)”.

The engagement is purely temporary on contractual basis and co-terminus with the project. There will be no provision of absorption of absorption/reemployment in IVRI/DBT on termination of the project.

No TA/DA will be provided for appearing in the interview and no separate letter will be issued.

A. Name tile of the project: “Centre for Agricultural bioinformatics (CABin)”.

B. Position/post to be filled: Research Associate (one)

C. Essential/Desirable qualifications:

•Essential: M.V.Sc./M.Tech./MSc Degree in Biotechnology/ Biochemistry/ Microbiology/Immunology/Bioinformatics/Genetics/Life Sciences or

Masters in Computer Application/ Masters in Computer science with first division.

• Desirable: Experience in cell culture, next generation sequencing, C++ and perl programming. NET/GATE qualified will be preferred.

• Experience : At least 2 years

D. Emoluments: Rs. 23000/- per month + 20% HRA

E. Age Limit: Maximum 40 years for men and 45 years for women

F. Duration of the project: Up to March 2017

G. Name of PI/Contact person: Dr. G.V.P.P.S. Ravi Kumar, Sr. Scientist, Division of Veterinary Biotechnology.

H. Address for correspondence: Dr. G.V.P.P.S. Ravi Kumar, Sr. Scientist, Computational Biology and Genomics facility,Division of Veterinary Biotechnology, I.V.R.I., Izatnagar – 243122

Advertisement: www.ivri.nic.in/jobs/WalkIn_interview_01042015.pdf

SALSA: A tool to scaffold long read assemblies with Hi-C

Jit — Fri, 15 Jun 2018 04:01:15 -0500

This code is used to scaffold your assemblies using Hi-C data. This version implements some improvements in the original SALSA algorithm. If you want to use the old version, it can be found in the old_salsa branch. To use the latest version, first run the following commands: cd SALSA make To run the code, you will need Python 2.7, BOOST libraries and Networkx(version lower than 1.2). If you consider using this tool, please cite our publication which describes the methods used for scaffolding. Ghurye, J., Pop, M., Koren, S., Bickhart, D., & Chin, C. S. (2017). Scaffolding of long read assemblies using long range contact information. BMC genomics, 18(1), 527. Link Ghurye, J., Rhie, A., Walenz, B.P., Schmitt, A., Selvaraj, S., Pop, M., Phillippy, A.M. and Koren, S., 2018. Integrating Hi-C links with assembly graphs for chromosome-scale assembly. bioRxiv, p.261149 Link For any queries, please either ask on github issue page or send an email to Jay Ghurye (jayg@cs.umd.edu).

Address of the bookmark: https://github.com/machinegun/SALSA

JRF Bioinformatics @ NATIONAL INSTITUTE OF IMMUNOLOGY NEW DELHI-110067

Fri, 17 Apr 2015 02:39:37 -0500

Applications are invited for the position of Junior Research Fellow (Project)/Senior Research Fellow (Project) for the following time-bound sponsored project as per the details given below:

1. “Development of bioinformatics methods for identifying novel secondary metabolites by genome mining” funded by DBT

JRF (P)/SRF (P) (One Position only)

Dr. Debasisa Mohanty Staff Scientist-VI deb@nii.res.in

Educational Qualifications: JRF (Project): M.Sc (Bioinformatics/ Biophysics/Biotechnology or any other stream of biological/physical sciences) or M.Tech. (Bioinformatics/Biotechnology/Computational Sciences) of M. Pharm.

SRF (Project): M.Sc (Bioinformatics/Biophysics/Biotechnology or any other stream of biological/physical sciences) or M.Tech. (Bioinformatics/Biotechnology/Computational Sciences) of M. Pharm with atleast 03 years of research experience.

Desirable Qualifications: Strong computer programming skills (in PERL/CGI/PHP or C++ or object oriented database management systems like MySQL etc or scripting languages under LINUX/UNIX environment) and sufficient experience in computational analysis of biological/biochemical data.

The candidates must highlight their experience in programming and database development in their CV. Job description: Computational analysis of genomes and development of bioinformatics tools and software’s for sequence and structure based analysis of biosynthetic pathways.

Emoluments: The selected candidates will draw consolidated emoluments as per Institute Rules, depending upon qualifications & experience JRF (Project): Rs. 12,000/- per month plus 30% HRA SRF (Project): Rs. 14,000/- per month plus 30% HRA (*Candidates possessing qualifications as per latest DST OM, will be given revised scales).

More at http://www1.nii.res.in/sites/default/files/projectappointments-Dr.DebasisaMohanty-30April2015.pdf

IGIB Recruitment 2015 – Project Scientist

Tue, 14 Apr 2015 12:19:59 -0500

IGIB Recruitment 2015 – Project Scientist & SPF Posts: CSIR – Institute of Genomics & Integrative Biology (IGIB) has issued notification for the recruitment of Project Scientist, Sr Project Fellow vacancies on temporary basis for project entitled “Setting up of CSIR(unit)-TRISUTRA (Translational research and Innovative Science through Ayurgenomics)”. Eligible candidates may apply in prescribed application format on or before 23-04-2015. Other details like age limit, educational qualification, selection process & how to apply are given below…

IGIB Vacancy Details:
Total No. of Posts: 04
Name of the Posts:
1. Project Scientist (Biology): 02 Posts
2. Project Scientist (Bioinformatics): 01 Post
3. Sr Project Fellow (Ayurveda): 01 Post

Age Limit: Candidates age should be 35 years for post 1, 32 years for post 2

Educational Qualification: Candidates should have Ph.D/ Ph.D submitted in any branch of Biological Science/ Life Science for post 1, Ph.D/ Ph.D submitted in Bioinformatics for post 2, BAMS degree with one year internship for post 3.

Selection Process: Candidates will be selected based on their performance in interview.

How to Apply: Eligible candidates may send their application along with all relevant documents on or before 23-04-2015.

Important Dates:
Last Date for Receipt of Application for Post 1 & 2: 23-04-2015.
Date of Interview for Post 3: 27-04-2015.

For other details like pay scale, age relaxation, educational qualification, selection process, how to apply, etc., click on the link given below…

http://www.freejobalert.com/wp-content/uploads/2015/03/Notification-IGIB-Project-Scientist-SPF-Posts.pdf

http://www.igib.res.in/sites/default/files/27042015.pdf