Project Scientist-I
Experimental / Computation analysis experience in highthroughput genomics/ clinical application.

Project Manager
Experience in handling large biological projects involving high-throughput genomics/ clinical application.

Scientific Administrative Assistant
Lab Work.

More at https://vinodscaria.genomes.in/positionsopen

The "Ifs" of NGS QC and Trimming

1. Ensures Data Integrity
   If you want to minimize errors in downstream analyses, QC and trimming remove low-quality reads and bases, ensuring high-confidence data. This step is essential for reliable variant calling, assembly, and other applications.

2. Removes Contaminants
   If adapter sequences or contaminants are present in the raw reads, trimming can eliminate them. This prevents issues like misalignment or incorrect biological interpretations, ensuring cleaner data for analysis.

3. Improves Mapping and Assembly
   If your goal is better alignment to a reference genome or improved de novo assembly, trimming low-quality bases and adapters is critical. High-quality reads map more efficiently and generate more accurate assemblies.

4. Reduces Computational Load
   If you want to save computational resources, trimming reduces the dataset size, which speeds up processing and analysis. Clean datasets mean less computational time spent on processing low-quality data.

5. Prepares for Standardized Analyses
   If your project involves multiple datasets, QC and trimming ensure uniformity across them. This standardization makes comparisons valid and reproducible, particularly in large collaborative studies.

The "Buts" of NGS QC and Trimming

1. Risk of Over-Trimming
   But excessive trimming can lead to the loss of informative sequences, reducing read depth and potentially discarding biologically relevant data. This is especially critical in studies with limited sequencing depth.

2. Bias Introduction
   But trimming algorithms might introduce biases, especially if they inadvertently remove sequences with specific biological patterns. This can skew results and compromise biological insights.

3. Loss of Context in Paired-End Reads
   But trimming one read in a pair more than the other can lead to loss of pairing information. This complicates downstream analyses that rely on paired-end data, such as structural variant detection.

4. Time and Resource Intensive
   But running QC and trimming for large datasets can be computationally expensive and time-consuming. As sequencing depth increases, preprocessing becomes a bottleneck in the analysis pipeline.

5. Variable Standards
   But the criteria for trimming (e.g., quality threshold, minimum read length) can vary between tools and datasets. This variability may affect reproducibility and comparability of results across studies.

Balancing the "Ifs" and "Buts"

To maximize the benefits of QC and trimming while mitigating the challenges, consider the following best practices:

• Use QC Tools Wisely: Start with tools like FastQC to identify quality issues in your raw data. Visualizing quality metrics helps tailor your trimming parameters.

• Choose Reliable Trimming Tools: Tools like Trimmomatic, Cutadapt, and BBduk offer adaptive and customizable trimming options. Select one that aligns with your dataset and project goals.

• Set Reasonable Parameters: Avoid over-trimming by setting quality thresholds and minimum read lengths that balance data retention and quality improvement.

• Test Downstream Effects: Validate the impact of QC and trimming on downstream analyses, such as alignment efficiency, variant calling accuracy, or assembly quality.

• Document Your Workflow: Maintain detailed records of the parameters and tools used for QC and trimming. This ensures reproducibility and enables better troubleshooting.

Conclusion

NGS quality control and trimming are essential steps to ensure reliable and accurate data for analysis. While the "ifs" highlight the clear benefits of these steps, the "buts" remind us of the potential pitfalls. By adopting best practices and carefully balancing these considerations, you can optimize your preprocessing workflow and unlock the full potential of your sequencing data.

(A Central University established by an Act of Parliament)

Prof. C.R.Rao Road, P.O. Central University Campus, Gachibowli,

Hyderabad - 500 046

Advt.No. UH/HR/Rectt-2015/02 dt. 12.10.2015

The University invites applications from the Indian citizens for the following positions:

Professor / Associate Professor / Assistant Professor :

Biotechnology & Bioinformatics

Last date : 16th November 2015

More Info : http://www.uohyd.ac.in/images/recruitment/advt-121015.pdf

Address of the bookmark: https://github.com/broadinstitute/VariantBam

JRF /1

Qualification : Master’s Degree in Biotechnology / life sciences with four years Bachelor’s Degree (or) Master’s Degree in Biotechnology / life sciences with NET qualification with 1st Division or 60% marks or equivalent overall grade point average . Non NET/ Master’s degree with three years Bachelor’s degree as per DST/DBT norms. Desirable: Working Experience in Molecular Biology Techniques, genome sequence analysis Bioinformatics

Emoluments : Rs.25000

SRF

Qualification : Master’s degree in Biotechnology/Bioinformatics/Life Science with 1st division or 60% marks or equivalent overall grade point average with 4 year of Bachelor’s degree or 5 years integrated Masters degree. Desirable: Working experience in Bioinformatics, genomic analysis

Emoluments : Rs.25000/

Age Limit: 35 years

Walk-in-interview will be held on 20th November 2015 at 10 AM at NRCPB, LBS Building, Pusa Campus, and New Delhi-110012

More at http://www.nrcpb.org/sites/default/files/Adverdisement_0.pdf

A swift, versatile phylogenomic and high-throughput sequencing simulator https://jackalope.lucasnell.com

Address of the bookmark: https://github.com/lucasnell/jackalope

Below mentioned teaching and non-teaching job vacancies are to be recruited.

Assistant Professors job vacancies in the disciplines of:
Biotechnology
Bioinformatics

Eligibility:
Masters Degree in the Discipline along with Ph.D

How to apply:
Candidates who possess requisite eligibility criteria for all the above mentioned positions required to apply on or before 10 days from the date of vacancy notification.

Candidates required to send copies of all the documents along with application

Contact details:
Uttaranchal College of Science & Technology
Nagal Hatnala, P.O. Kulhan Sahastradhara Road,
Dehradun-248001, Uttarakhand, India

More details can be had at:
Dehradun Classifieds e-paper dated 01.11.2015 at page number 40-41

About Employer

Uttaranchal College of Science and Technology, Dehradun, Uttarakhand is affiliated to HNB Garhwal University, Srinagar, Uttarakhand

Employer: Uttaranchal College of Science & Technology
Address: Uttaranchal College of Science & Technology Nagal Hatnala, P.O. Kulhan Sahastradhara Road, Dehradun-248001, Uttarakhand, India
Email: info@ucstdoon.com
URL: http://www.ucstdoon.com
Phone: 0135-2607011, 607413, 3254785, 09719146701

Project Assistant II Jobs opportunity in National Chemical Laboratory (NCL) on temporary basis

Project Code No. : GAP312626

Title of the Project : Microbial ecology and distribution of geochemical cycling genes in an hot spring ecosystem

No. of Post : 01

Qualifications : M.Sc./B.Tech/M.Tech in Computational biology/ Bioinformatics from recognized university with minimum 60 % marks (aggregate)

Desirable : Good knowledge of computational skills, Linux (command line and GUI) and Unix; Perl / Python / R /C-programming. Practical knowledge of analysis of Next generation sequence datasets (amplicon sequencing, whole metagenome, and complete genome sequencing) with reference to microbes. Analysis and statistical validation of NGS data generated from different chemistry platforms. Some wet-lab experience in microbial system would be an added advantage as project involves some travel.

Emoluments : Rs. 16,000/-

Age Limit : 28 years
How to apply

The application with the above information duly signed together with photo-copies of relevant certificates/testimonials should be addressed to : The Head, NCIM Resource Centre (Attn Dr. M.S. DHARNE), National Chemical Laboratory, Pune 411 008, so as to reach on or before 16th November 2015.

More at http://www.ncl-india.org/files/JoinUs/JobVacancies/TemporaryJobs.aspx?menuid=ql6&childmenustripid=divSubQL6

We are seeking an individual with expertise in analyzing next generation sequencing data, mutation calling in exome seq data, network inference, data integration, and modeling. Competitive candidates would also have programming experience in scripting languages with perl, C, C++, and R programming. This position requires a PhD in Computational Biology, Bioinformatics, Biostatistics or related fields, and evidence of scientific productivity through publications in international journals. Motivation to gain an in-depth understanding of biological phenomena is required! Applications should include a current CV and names of at least three references. Application packages and inquiries regarding this position can be sent to Dr. Dasaradhi Palakodeti ( dasaradhip@instem.res.in ) or Dr. Colin Jamora ( colinj@instem.res.in). Screening of applications will commence immediately and the position will remain open until filled.

More at https://www.instem.res.in/open-positions

Essential Qualifications:
First class M. Tech or M.E. in computer science or information technology or bioinformatics with 4 years of R&D experience (after Master’s Degree) in an R&D organization with work profile of large data assembly, analysis, and/or customer/user-based software programming and/or, program implementation, database development evidenced by patents and/or publications, credible scale of R&D related data/data sets submission to public database for expanded use etc. Company Info.
Centre for Innovative and Applied Bioprocessing

CENTER OF INNOVATIVE AND APPLIED BIOPROCESSING (A National Institute under Dept. of Biotechnology, Ministry of Science & Technology, Govt. of India) 2nd Floor, C-127, Phase VIII, Industrial Area, S.A.S. Nagar, Mohali-160071(Pb). Additional Information States & U.T State & Union Territories Punjab How To Apply Apply Details
Apply directly.. Web/Notification URL http://ciab.res.in/vacancies/CIAB-Rollin g%20Advt-2015.pdf