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	<title><![CDATA[BOL: Related items]]></title>
	<link>https://bioinformaticsonline.com/related/28805?offset=90</link>
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	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/31089/conpade-genome-assembly-ploidy-estimation-from-next-generation-sequencing-data</guid>
	<pubDate>Fri, 24 Feb 2017 04:55:41 -0600</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/31089/conpade-genome-assembly-ploidy-estimation-from-next-generation-sequencing-data</link>
	<title><![CDATA[ConPADE: Genome Assembly Ploidy Estimation from Next-Generation Sequencing Data]]></title>
	<description><![CDATA[<p><span>ConPADE (Contig Ploidy and Allele Dosage Estimation), a probabilistic method that estimates the ploidy of any given contig/scaffold based on its allele proportions. In the process, they report findings regarding errors in sequencing. The method can be used for whole genome shotgun (WGS) sequencing data. They also show applicability of the method for variant calling and allele dosage estimation. Results for simulated and real datasets are discussed and provide evidence that ConPADE performs well as long as enough sequencing coverage is available, or the true contig ploidy is low.&nbsp;</span></p>
<p><span>https://github.com/microsoftgenomics</span></p><p>Address of the bookmark: <a href="https://github.com/microsoftgenomics" rel="nofollow">https://github.com/microsoftgenomics</a></p>]]></description>
	<dc:creator>Jit</dc:creator>
</item>
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	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/31295/mycc-accurate-binning-of-metagenomic-contigs-via-automated-clustering-sequences-using-information-of-genomic-signatures-and-marker-genes</guid>
	<pubDate>Fri, 03 Mar 2017 08:34:23 -0600</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/31295/mycc-accurate-binning-of-metagenomic-contigs-via-automated-clustering-sequences-using-information-of-genomic-signatures-and-marker-genes</link>
	<title><![CDATA[MyCC: Accurate binning of metagenomic contigs via automated clustering sequences using information of genomic signatures and marker genes]]></title>
	<description><![CDATA[<p><span>MyCC, an automated binning tool that combines genomic signatures, marker genes and optional contig coverages within one or multiple samples, in order to visualize the metagenomes and to identify the reconstructed genomic fragments.</span></p>
<p><span>More at&nbsp;http://www.nature.com/articles/srep24175</span></p><p>Address of the bookmark: <a href="https://sourceforge.net/projects/sb2nhri/files/MyCC/" rel="nofollow">https://sourceforge.net/projects/sb2nhri/files/MyCC/</a></p>]]></description>
	<dc:creator>Jit</dc:creator>
</item>
<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/31353/concoct-clustering-contigs-with-coverage-and-composition</guid>
	<pubDate>Mon, 06 Mar 2017 04:08:16 -0600</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/31353/concoct-clustering-contigs-with-coverage-and-composition</link>
	<title><![CDATA[CONCOCT: Clustering cONtigs with COverage and ComposiTion]]></title>
	<description><![CDATA[<p>A program for unsupervised binning of metagenomic contigs by using nucleotide composition, coverage data in multiple samples and linkage data from paired end reads.</p>
<p>Warning! This software is to be considered under development. Functionality and the user interface may still change significantly from one version to another. If you want to use this software, please stay up to date with the list of known issues:<a href="https://github.com/BinPro/CONCOCT/issues">https://github.com/BinPro/CONCOCT/issues</a></p><p>Address of the bookmark: <a href="https://github.com/BinPro/CONCOCT" rel="nofollow">https://github.com/BinPro/CONCOCT</a></p>]]></description>
	<dc:creator>Jit</dc:creator>
</item>
<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/31382/seqmule-automated-human-exomegenome-variants-detection</guid>
	<pubDate>Tue, 07 Mar 2017 10:12:36 -0600</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/31382/seqmule-automated-human-exomegenome-variants-detection</link>
	<title><![CDATA[SeqMule: Automated human exome/genome variants detection]]></title>
	<description><![CDATA[<p><span>SeqMule takes single-end or paird-end FASTQ or BAM files, generates a script consisting of more than 10 popular alignment, analysis tools and runs the script line by line. Users can change the pipeline or fine-tune the parameters by modifying its configuration file. SeqMule also has some built-in functions, such as pooling consensus calls from various callers, plotting a Venn diagram showing intersection among different callers, and downloading databases. SeqMule can be used for both Mendelian disease study and cancer genome study.</span></p><p>Address of the bookmark: <a href="http://seqmule.openbioinformatics.org/en/latest/" rel="nofollow">http://seqmule.openbioinformatics.org/en/latest/</a></p>]]></description>
	<dc:creator>Abhimanyu Singh</dc:creator>
</item>
<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/32485/bacterial-genome-assembly</guid>
	<pubDate>Fri, 05 May 2017 06:11:22 -0500</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/32485/bacterial-genome-assembly</link>
	<title><![CDATA[Bacterial genome assembly !!]]></title>
	<description><![CDATA[<p>This tutorial will serve as an example of how to use free and open-source genome assembly and secondary scaffolding tools to generate high quality assemblies of&nbsp;bacterial sequence data. The bacterial sample used in this tutorial will be referred&nbsp;to simply&nbsp;as &ldquo;Species&rdquo; since it is&nbsp;live data. This data is paired-end data, meaning that there are forward and reverse reads, which we will designate as Sample_R1.fastq and Sample_R2.fastq, respectively.</p>
<p>https://github.com/jennomics/WorkflowPaper/blob/master/Genome%20Assembly%20and%20Annotation.md</p><p>Address of the bookmark: <a href="http://bioinformatics.uconn.edu/bacterial-genome-assembly-tutorial/" rel="nofollow">http://bioinformatics.uconn.edu/bacterial-genome-assembly-tutorial/</a></p>]]></description>
	<dc:creator>Jit</dc:creator>
</item>
<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/news/view/32719/download-assemblies-from-ncbi</guid>
	<pubDate>Mon, 15 May 2017 06:02:32 -0500</pubDate>
	<link>https://bioinformaticsonline.com/news/view/32719/download-assemblies-from-ncbi</link>
	<title><![CDATA[Download assemblies from NCBI]]></title>
	<description><![CDATA[<p>A new &ldquo;Download assemblies&rdquo; button is now available in the&nbsp;<a href="https://www.ncbi.nlm.nih.gov/assembly" target="_blank">Assembly</a>&nbsp;database. This makes it easy to download data for multiple genomes without having to write scripts.</p><p>For example, you can run a search in Assembly and use check boxes (see left side of screenshot below) to refine the set of genome assemblies of interest. Then, just open the &ldquo;Download assemblies&rdquo; menu, choose the source database (<a href="https://www.ncbi.nlm.nih.gov/genbank/" target="_blank">GenBank</a>&nbsp;or&nbsp;<a href="https://www.ncbi.nlm.nih.gov/refseq/" target="_blank">RefSeq</a>), choose the file type, and start the download. An archive file will be saved to your computer that can be expanded into a folder containing your selected genome data files.</p><p><img src="https://ncbiinsights.files.wordpress.com/2017/05/download_button.jpg?w=584" alt="image" width="584" height="444" style="border: 0px; border: 0px;"></p><p>&nbsp;</p><p>More at&nbsp;https://ncbiinsights.ncbi.nlm.nih.gov/2017/05/08/genome-data-download-made-easy/</p>]]></description>
	<dc:creator>Bulbul</dc:creator>
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	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/36594/fragscaff-genome-assembly-with-contiguity-preserving-transposition</guid>
	<pubDate>Mon, 14 May 2018 04:28:14 -0500</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/36594/fragscaff-genome-assembly-with-contiguity-preserving-transposition</link>
	<title><![CDATA[fragScaff: Genome Assembly with Contiguity Preserving Transposition]]></title>
	<description><![CDATA[<p>Contiguity preserving transposition and sequencing (CPT-seq) is an entirely in vitro means of generating libraries comprised of 9216 indexed pools, each of which contains thousands of sparsely sequenced long fragments ranging from 5 kilobases to &gt;1 megabase. This software, fragScaff, leverages coincidences between the content of different pools as a source of contiguity information for scaffolding de novo genome assemblies. FragScaff is complementary to Lachesis, providing midrange contiguity to support robust, accurate chromosome-scale de novo genome assemblies without the need for laborious in vivo cloning steps.</p>
<p>Further information about fragScaff, including source code, is available at:<a href="https://sourceforge.net/projects/fragscaff/files/">https://sourceforge.net/projects/fragscaff/files</a>.</p>
<p>Manuscript describing fragScaff was published as: Adey A, Kitzman JO, Burton JN, Daza R, Kumar A, Christiansen L, Ronaghi M, Amini S, L Gunderson K, Steemers FJ, Shendure J#.&nbsp;<em>In vitro, long-range sequence information for de novo genome assembly via transposase contiguity.</em>&nbsp;Genome Research 2014 Dec;24(12):2041-9. doi:&nbsp;<a href="http://dx.doi.org/10.1101/gr.178319.114">10.1101/gr.178319.114</a>. PubMed PMID:&nbsp;<a href="http://www.ncbi.nlm.nih.gov/pubmed/25327137">25327137</a>.</p>
<p>&nbsp;</p><p>Address of the bookmark: <a href="https://sourceforge.net/projects/fragscaff/files/" rel="nofollow">https://sourceforge.net/projects/fragscaff/files/</a></p>]]></description>
	<dc:creator>Jit</dc:creator>
</item>
<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/22961/bioscripts</guid>
	<pubDate>Sun, 28 Jun 2015 07:46:14 -0500</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/22961/bioscripts</link>
	<title><![CDATA[BioScripts]]></title>
	<description><![CDATA[<p>You are requested to please bookmark collection of bioinformatics tools, scripts, codes that can be pieced together in a very easy and flexible manner to perform both simple and complex bioinformatics tasks.</p>
<p>The next-generation sequencing included whole genome sequencing(WGS), transcriptome sequencing (whole cDNA sequencing, RNA-seq), digital gene expression sequencing (Tag-Seq), ChIP-Seq, and so on. And there are many sequencing platform to generate sequece, as well know Sanger/ABi(the frist generation), Solexa/illumina, SOLiD/ABi, 454/Roche. But thier sequence format is different, also they have different error type. High quality data is very important for further analysis or data mining. There are many pipeline for raw sequence quality analysis and control with few of process for reporting reads quality statistical details, trimming, filtering, and error correction. Please bookmarks them for the benefits of bioinformatics community.</p>
<p>https://code.google.com/p/biowiki/</p>
<p>https://code.google.com/p/ngs-pipeline/source/browse/#svn%2Ftrunk</p>
<p>NGSand Perl scripts https://code.google.com/hosting/search?q=NGS+perl&amp;projectsearch=Search+projects</p>
<p>NGS and Python scripts https://code.google.com/hosting/search?q=NGS+Python&amp;projectsearch=Search+projects</p><p>Address of the bookmark: <a href="https://code.google.com/hosting/search?q=bioinformatics&amp;sa=Search" rel="nofollow">https://code.google.com/hosting/search?q=bioinformatics&amp;sa=Search</a></p>]]></description>
	<dc:creator>Rahul Nayak</dc:creator>
</item>
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	<guid isPermaLink="true">https://bioinformaticsonline.com/blog/view/22995/bioinformatics-phd-postdoc-job-rejection</guid>
	<pubDate>Thu, 02 Jul 2015 08:52:46 -0500</pubDate>
	<link>https://bioinformaticsonline.com/blog/view/22995/bioinformatics-phd-postdoc-job-rejection</link>
	<title><![CDATA[Bioinformatics PhD / PostDoc / Job Rejection]]></title>
	<description><![CDATA[<div><p>While your PhD or PostDoc application, it is more common that you got rejected by many professors. Don't disappoint reply it calmly.</p><p><img src="http://bioinformaticsonline.com/mod/photo/rejected1.png" alt="image" style="border: 0px; border: 0px;"></p><p>In grad school, I shared a house with three Bioinformatics PhD students. One, when he applied to a particular professor, received a letter that said, essentially, "If you are applying because you want to enrich yourself, great. If you are applying because you want a job, you should know that you won't get one." I am trying to tell you this is because if you, with a good background in Bioinformatics, are passing up opportunities, you must be a strong candidate in many areas. Enrich yourself.<br /><br /> So, my suggestion is take a deep breath, forgot about all. Don&rsquo;t take it personally. It's been usual processes while hunting for a good lab and professor. Take is positive, I am not sure why they reject, but don't worry perhaps the lab don't deserve you. Always remember there are billions of reasons not to hire someone for projects, especially in a research sector.<br /><br /> My suggestion, please do not whine about how you were a great research candidate for the post, and you just can't understand why they were so stupid as to have rejected you! This feeling will not win you any points in research, community. Especially, when in todays socially connected era everyone is linked. Remember, a nice E-mail saying, "I really wished to working with you on this project and I hope we cross paths again," is all you need to send to the professor. Send a thank you note to the professor. Thank them for the time they spend to judge you. In the future, If you and the professor (of your dream) are attending a bioinformatics conference, invite him/her to lunch (please remember to pay the bill). In today evolving scientific ere, always remember to build your solid network in order to get a job of interest. Join all possible networking sites like LinkedIn, ResearchGate, Acamedia, FB for the same reason. You as a researcher always build a bridge with student/researcher/colleague/professor who have the research potential to lead in research and hire you. Just because you didn't get this project, doesn't mean there isn't another that will open up in couple of month.<br /><br /> Mostly, jobs that are hard to get are hard to get. Only you can decide if the continued sacrifices are worth the expected payout. If it is, keep on plowing. Build relationships. Attend conferences.</p><p>Image ref @ JaSonYa</p></div>]]></description>
	<dc:creator>Jit</dc:creator>
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  <guid isPermaLink='true'>https://bioinformaticsonline.com/researchlabs/view/23209/bisr-jaipur</guid>
  <pubDate>Tue, 07 Jul 2015 23:12:26 -0500</pubDate>
  <link></link>
  <title><![CDATA[BISR Jaipur]]></title>
  <description><![CDATA[
<p>The Bioinformatics Centre at BISR has created an infrastructure for providing facilities to the users working in the field of Biological Sciences. The users of Rajasthan, Jaipur in particular, are using facilities available at the Bioinformatics Centre extensively. The centre has leased line Internet connection as well latest Bioinformatics software for sequence and structure analysis. The centre provides the following services:</p>

<p>    Bioinformatics supports to researchers<br />    Customized training in Bioinformatics for researchers and faculty members<br />    Support in Installing, implementing and maintaining software on computer.<br />    Create awareness for taking preventive measure against data security<br />    Organize workshops on thrust ares of Bioinformatics<br />    Research Training to students of Biotechnology and Bioinformatics </p>

<p>More at http://bioinfo.bisr.res.in/index.php</p>
]]></description>
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