BOL: Related items

RNA-seq workflow: gene-level exploratory analysis and differential expression

Jit — Sat, 09 Oct 2021 07:59:23 -0500

Here we walk through an end-to-end gene-level RNA-seq differential expression workflow using Bioconductor packages. We will start from the FASTQ files, show how these were quantified to the reference transcripts, and prepare gene-level count datasets for downstream analysis. We will perform exploratory data analysis (EDA) for quality assessment and to explore the relationship between samples, perform differential gene expression analysis, and visually explore the results.

Address of the bookmark: http://master.bioconductor.org/packages/release/workflows/vignettes/rnaseqGene/inst/doc/rnaseqGene.html

Scientist position in Structural Bioinformatics at Lonza

Wed, 03 Feb 2021 21:38:06 -0600

Lonza (https://www.lonza.com/) are seeking a highly motivated and skilled (Senior) Scientist with experience in Structure-based Protein Engineering and Bioinformatics to join Lonza's Applied Protein Services (APS) Bioinformatics team based in Cambridge, UK.

More at https://instruct-eric.eu/jobs/scientist-position-in-structural-bioinformatics-at-lonza-cambridge-uk/

Tools for Differential expression analysis

Abhi — Tue, 08 Nov 2022 03:40:33 -0600

apeglm - https://bioconductor.org/packages/release/bioc/html/apeglm.html

ashr - https://github.com/stephens999/ashr, https://cran.r-project.org/web/packages/ashr/index.html

consensusDE - https://bioconductor.org/packages/release/bioc/html/consensusDE.html

DESeq2 - https://bioconductor.org/packages/release/bioc/html/DESeq2.html

edgeR - https://bioconductor.org/packages/release/bioc/html/edgeR.html

limma - https://kasperdanielhansen.github.io/genbioconductor/html/limma.html https://bioconductor.org/packages/release/bioc/html/limma.html

MetaCycle - https://cran.r-project.org/web/packages/MetaCycle/index.html, https://github.com/gangwug/MetaCycle

RUVSeq - https://bioconductor.org/packages/release/bioc/html/RUVSeq.html

SARTools - https://github.com/PF2-pasteur-fr/SARTools

tximport - https://github.com/mikelove/tximport

Bioinformatics in Africa: Part6 - Sudan

BioStar — Sat, 06 Feb 2021 21:20:59 -0600

Commission for Biotechnology & Genetic Engineering Khartoum: The Commission for Biotechnology and Genetic Engineering was established in 9/2/1993 as research unit. In addition to research activities it acts as focal point for the International Center for Biotechnology and Genetic Engineering. The commission conducts researches in order to play a part in solving economical, environmental, health and nutritional problems using modern research techniques with an emphasis on the applied researches in these areas. The laboratories were well furnished with the essential equipments and the catalyst infrastructure to facilitate emergence of a successful for research. The Commission equipped with a computer center and information to serve as informatics and Digital library.

Research Interest and Activities: 1. Plant Genetic Transformations
2. Molecular Population Genetics 3. Detection of human and Animals diseases 4. Breast Cancerspecific protein marker 5. Phytochemical 6. Genomic map 7. Bioremediation 8. Tissue Culture.

Web site and links: www.geocity.cbge.com

Bactopia: a flexible pipeline for complete analysis of bacterial genomes

Abhi — Sat, 08 Jun 2024 16:25:08 -0500

Bactopia is a flexible pipeline for complete analysis of bacterial genomes. The goal of Bactopia is process your data with a broad set of tools, so that you can get to the fun part of analyses quicker!

Bactopia was inspired by Staphopia, a workflow we (Tim Read and myself) released that is targeted towards Staphylococcus aureus genomes. Using what we learned from Staphopia and user feedback, Bactopia was developed from scratch with usability, portability, and speed in mind from the start.

Bactopia uses Nextflow to manage the workflow, allowing for support of many types of environments (e.g. cluster or cloud). Bactopia allows for the usage of many public datasets as well as your own datasets to further enhance the analysis of your sequencing. Bactopia only uses software packages available from Bioconda and Conda-Forge to make installation as simple as possible for all users.

To highlight the use of Bactopia and Bactopia Tools, we performed an analysis of 1,664 public Lactobacillus genomes, focusing on Lactobacillus crispatus, a species that is a common part of the human vaginal microbiome. The results from this analysis are published in mSystems under the title: Bactopia: a flexible pipeline for complete analysis of bacterial genomes

Address of the bookmark: https://bactopia.github.io/latest/

Bioinformatics Research Scientist @ Oklahoma State University (OSU)

Tue, 17 Aug 2021 13:24:39 -0500

This position is an early career research scientist in the area of Bioinformatics to support research projects involving faculty and staff, at Oklahoma State University (OSU). This is a highly technical position that requires a strong research background in biomedical or life sciences, including a high level of expertise with bioinformatics algorithms, databases, and analyses with a focus on next-generation sequence data. Although most of the projects will deal directly with the analysis of DNA and RNA sequence data the individual should be well versed in other types of data sources as well (i.e., microarrays) and handling of large datasets (using data analytics, machine learning, and deep learning techniques).

More at https://okstate.csod.com/ats/careersite/JobDetails.aspx?site=8&id=9874

SLR-superscaffolder: A scaffold assemble pipeline for stLFR reads.

Jit — Fri, 14 Feb 2020 14:23:30 -0600

This is a scaffold assembler designed for stLFR reads[1]. It uses the link-reads information from stLFR reads to assemble contigs to scaffolds.

Here is an illustration of this pipeline:

Address of the bookmark: https://github.com/BGI-Qingdao/SLR-superscaffolder

Getting Started with Nextflow

LEGE — Sat, 18 Sep 2021 01:28:41 -0500

Introduction to Bioinformatics workflows with Nextflow and nf-core

Getting Started with Nextflow

Objectives Understand

What a workflow management system is.

Understand the benefits of using a workflow management system.

Explain the benefits of using Nextflow as part of your bioinformatics workflow.

Explain the components of a Nextflow script.

Run a Nextflow script.

Introduction to Bioinformatics workflows with Nextflow and nf-core

Getting Started with Nextflow

Address of the bookmark: https://carpentries-incubator.github.io/workflows-nextflow/aio/index.html

Python vs Perl

Rahul Agarwal — Thu, 11 Jul 2013 14:39:19 -0500

Why bioinformatician still using Perl when Python is easy to code, good in ReXp and faster than perl?

Junior Research Fellow (JRF)

Sat, 13 Jul 2013 11:17:22 -0500

School of Biotechnology
Gautam Buddha University
Greater Noida, UP - 201310

Applications are invited for one position of Junior Research Fellow (JRF) in a Department of Biotechnology (DBT) sponsored research project entitled “Design, synthesis and evaluation of potent aminopeptidase inhibitors for malarial therapy” under the supervision of Dr. Shakti Sahi.

The monthly fellowship of JRF will be Rs 12,000/- plus HRA as per the University rules.

Essential Qualification: Master degree in any discipline of Life Science with NET qualified.

Desirable Qualification: Preference will be given to candidates having research experience in in silico drug designing/Bioinformatics.

Interested candidates may send their resume to undersigned on or before 14th July 2013 by post-mail/e-mail shaktis@gbu.ac.in or shaktisahi@gmail.com. No TA and DA will be paid for appearing for the interview. Dr. Shakti Sahi (Principle Investigator)

Advertisement:
www.gbu.ac.in/Recruitment/JRF_Advt_DBTProject_Shakt