BOL: Related items

VariantBam: Filtering and profiling of next-generational sequencing data using region-specific rules

Rahul Nayak — Thu, 04 Oct 2018 16:30:44 -0500

VariantBam is a tool to extract/count specific sets of sequencing reads from next-generational sequencing files. To save money, disk space and I/O, one may not want to store an entire BAM on disk. In many cases, it would be more efficient to store only those read-pairs or reads who intersect some region around the variant locations. Alternatively, if your scientific question is focused on only one aspect of the data (e.g. breakpoints), many reads can be removed without losing the information relevant to the problem.

Address of the bookmark: https://github.com/broadinstitute/VariantBam

R for Microsoft Excel

Jitendra Narayan — Wed, 18 Feb 2015 00:43:27 -0600

If you currently use a spreadsheet like Microsoft Excel for data analysis, you might be interested in taking a look at this tutorial on how to transition from Excel to R by Tony Ojeda. The tutorial explains how to use R functions in place of Excel formulas, including tools like =AVERAGE and =VLOOKUP. For the most part, it uses modern R packages to keep the R code clear and concise.

You'll likely still be using Excel as a data source, though, so you'll also want to check out this guide to importing data from Excel to R from MilanoR.

Reference http://www.r-bloggers.com/an-r-tutorial-for-microsoft-excel-users/

jackalope: A swift, versatile phylogenomic and high-throughput sequencing simulator

Abhimanyu Singh — Fri, 26 Jul 2019 00:58:12 -0500

jackalope simply and efficiently simulates (i) variants from reference genomes and (ii) reads from both Illumina and Pacific Biosciences (PacBio) platforms. It can either read reference genomes from FASTA files or simulate new ones. Genomic variants can be simulated using summary statistics, phylogenies, Variant Call Format (VCF) files, and coalescent simulations—the latter of which can include selection, recombination, and demographic fluctuations. jackalope can simulate single, paired-end, or mate-pair Illumina reads, as well as reads from Pacific Biosciences These simulations include sequencing errors, mapping qualities, multiplexing, and optical/PCR duplicates. All outputs can be written to standard file formats.

A swift, versatile phylogenomic and high-throughput sequencing simulator https://jackalope.lucasnell.com

Address of the bookmark: https://github.com/lucasnell/jackalope

A guide for complete R beginners :- R Syntax

Archana Malhotra — Fri, 20 Feb 2015 23:41:03 -0600

R is a functional based language, the inputs to a function, including options, are in brackets. Note that all dat and options are separated by a comma

Function(data, options)

Even quit is a function

So is help

help(read.table)

Provides the help page for the FUNCTION ‘read.table’

help.search(“t test”)

Searches for help pages that might relate to the phrase ‘t test’

NOTE: quotes are needed for search strings, they are not needed when referring to data objects or function names.

There is a short cut for help,

? shows the help page on a function name, same as help(function)

?read.table

?? searches for help pages on functions, same as help.search(‘phrase’)

??“t test”

Information is usually returned from a function, by default this is printed to screen

read.table(‘data.tsv’)

This can always be stored, we call what it is stored in an ‘object’

mydata

here mydata is an object of type dataframe

Reminder:

Vector: a list of numbers, equivalent to a column in a table
Data Frame = a collection of vectors. Equivalent to a table

Hint:

Up/Down arrow keys can be use to cycle through previous commands

genomics public data links !

Jit — Thu, 13 Feb 2020 00:20:00 -0600

List of publically available databases on google server.

More at https://software.broadinstitute.org/gatk/download/bundle

ftp://ftp.ncbi.nlm.nih.gov/snp/organisms/human_9606/VCF/GATK/.

ftp://ftp.broadinstitute.org/bundle/hg38/hg38bundle/

Address of the bookmark: https://console.cloud.google.com/storage/browser/genomics-public-data/resources/broad/hg38/v0?pli=1

Asst. Professor at Central University of Jharkhand (CUJ)

Sun, 01 Mar 2015 01:17:52 -0600

Central University of Jharkhand (CUJ) has issued a recruitment notification for the recruitment of Assistant Professor through recruitment notification – Central University of Jharkhand (CUJ) Recruitment 2015 – Advt. No.: CUJ/Advt./14-15/15 Date: 26th Feb. 2015. Candidates who have completed M.Sc, Ph.D can apply for the new recruitment notification from Central University of Jharkhand (CUJ)

Central University of Jharkhand has been granted funds by the Department of Biotechnology (DBT), Govt. of India to establish “DBT-Boost to CUJ Interdisciplinary Life Sciences Departments for Education and Research” Applications are invited for the Assistant Professor on purely temporary basis. The appointments shall be initially for a period of one year, renewable every year depending on the satisfactory performance, till the end of project.

Position: ASSISTANT PROFESSOR (Total 03)
Salary: 45,000/- (fixed) per month
Essential Qualifications: i. Good academic record with at least 55% marks (or an equivalent grade in a point scale wherever grading system is followed) at the master’s degree level with specialization in Biodiversity and Systematic/ Systems Biology/ Biophysics/ Bioinformatics from an Indian University, or an equivalent degree from an accredited foreign university. ii. Besides fulfilling the above qualifications, the candidates must have cleared the National Eligibility Test (NET) conducted by the UGC, CSIR or similar test accredited by the UGC like SLET/SET. iii. Notwithstanding anything contained in i. and ii. candidates, who are or have been awarded Ph.D Degree in accordance with the University Grants Commission (Minimum Standards and Procedure for Award of Ph.D. Degree) Regulation, 2009, shall be exempted from therequirement of the minimum eligibility condition of NET/SLET/SET for recruitment and appointment of Assistant Professor. iv. NET/SLET/SET shall also not be required for such disciplines for which NET/SLET/SET in not conducted.
Desirable: Preference will be given to candidates having Ph.D in any of the above mentioned areas with NET

IMPORTANT DATES TO REMEMBER :

Last Date to Apply for this job 24/3/2015

REFERENCE:

Central University of Jharkhand (CUJ) Recruitment 2015 – Advt. No.: CUJ/Advt./14-15/15 Date: 26th Feb. 2015.

More at http://cuj.ac.in/careers.php

Tools for Protein-Protein Docking !

Poonam Mahapatra — Wed, 25 Apr 2018 05:15:53 -0500

Predicting the structure of protein–protein complexes using docking approaches is a difficult problem whose major challenges include identifying correct solutions, and properly dealing with molecular flexibility and conformational changes. Following are the tools to predict the structure of protein–protein complexes:

3D-Dock Suite

Global rigid search: FFTShape complementarity and electrostatics

Re-scoring and clustering. Refinement of interface side-chains

3D-Garden

Global rigid search in ensamble

Shape complementarity and Lennard–Jones potential

Side chain and backbone dihedral refinement

DOT

Global rigid search: FFTShape complementarity, electrostatics and VDWNone

Escher NG

Global rigid searchShape complementarity, hydrogen bonds and electrostatic

Integrated in VEGA

GRAMM

Global rigid search: FFT. smooth protein surface representation for soft docking

Shape complementarity and Lennard-Jones potential

Clustering of conformations

GRAMM-X

Global rigid search: FFT. smooth protein surface representation for soft docking

Shape complementarity and Lennard-Jones potentialminimization and re-scoring with multiple filters

HEX

Global rigid search: Fourier correlation of spherical harmonics

Shape complementarity

HADDOCK

Global rigid searchElectrostatic ,VDW and desolvation energy termsMD simulated annealing refinement . Filtering based on external data.

ICM

Global rigid search: Monte CarloEmpirical scoring function

Clustering and selection of conformations. Refinement of interface side-chains and re-scoring

MolFit

Global rigid search: FFTShape complementarity

Clustering of good solutions, filtering using a priori information and small, local rigid rotations around selected conformations

PatchDock

Global rigid searchShape complementarity and atomic desolvation energy

Clustering of conformations

PyDock

Global rigid search:FFTShape complementarity

rescoring by binding electrostatics and desolvation energy

RosettaDock

Local rigid search: Monte Carlo with low and high resolution structure representation levels

Different scoring parameters for the different resolutions

ZDOCK

Global rigid search: FFTShape complementarity, desolvation energy, and electrostatics.

Energy minimization and re-scoringFree for academics

Point to note:

The proper treatment of flexibility in protein–protein docking is still an active field of research. You first should analyzed your proteins in order to define their conformational space and then choose the most suitable method for your docking problem.

RA Bioinformatics at Ch. Charan Singh University, Meerut

Wed, 11 Mar 2015 09:07:07 -0500

Ch. Charan Singh University, Meerut

http://molbiolabccsumrt.webs.com/

Applications are invited for one post of RA in a DBT funded research project “Creation of Bioinformatics Infrastructure Facility (BIF) for the promotion of Biology Teaching through Bioinformatics (BTBI) Scheme of BTISet”.

Candidate should have a Ph.D. degree in Bioinformatics/Biotechnology/Genetics and Plant Breeding with adequate experience in the area of Bioinformatics. If a suitable candidate for the post of RA is not available, a JRF/SRF may be appointed.

Candidate for the post of JRF/SRF should have Master’s degree in relevant subject with adequate experience in the area of Bioinformatics and should be NET/DBT-BINC qualified

Interested candidates may send their bio-data to Prof. H. S. Balyan (hsbalyan@gmail.com) (in exceptional case, bio-data may also be submitted at the time of interview) and attend the interview on Monday, March 30, 2015 at 11:00 AM in the Department of Genetics & Plant Breeding, Ch. Charan Singh University, Meerut. Candidates shall bring their original documents at the time of interview for verification. No interview letters will be issued and no TA/DA will be paid.

Coding Ground

Jitendra Narayan — Tue, 17 Mar 2015 00:47:20 -0500

Online coding group for most of the programming languages.

Code in almost all popular languages using Coding Ground. Edit, compile, execute and share your projects, 100% cloud.

http://www.tutorialspoint.com/codingground.htm

Address of the bookmark: http://www.tutorialspoint.com/codingground.htm

Bioinformatics Project Assistant at NITW

Wed, 01 Apr 2015 01:43:27 -0500

NATIONAL INSTITUTE OF TECHNOLOGY WARANGAL – 506 004

DEPARTMENT OF BIOTECHNOLOGY

No. NITW/Bio/ Date: 30th March 2015

ADVERTISEMENT FOR PROJECT FELLOW (Research Staff)

Applications are invited for the post of Project Fellow (Research Staff) for the project entitled ‘Metagenome derived nitroreductases for degradation of nitro compounds’ sponsored by the Department of Science and Technology (DST-INSPIRE), Govt. of India.

Position: Project Fellow (1 No.)

Project Duration: 5 years

Note: The post is purely on temporary basis for a period one year and may be extendable till the end of the project based on the progress of the candidate.

Emoluments: Rs. 14,000/- per month (Consolidated) for first two years and Rs. 16,000/- per month (Consolidated) for next three years.

Essential Qualifications:

i) First class in M.Tech/M.S (Biotechnology/Industrial Biotechnology/Bioinformatics) or equivalent. Or

ii) First class in M.Sc. (Biotechnology/Bioinformatics/Biochemistry/ Microbiology/Molecular biology).

Desirable Qualification: Preference will be given to candidates who have cleared NET/GATE or having prior work experience in Molecular biology/Bioinformatics sequence analysis.

Interested candidates may submit their application in plain paper along with Curriculum Vitae and photocopies of certificates in support of educational/professional qualifications. Application should be sent in a closed cover with a superscription on the cover “Application for the post of project fellow (DST-INSPIRE)” on or before 20.04.2015 (Monday) via Post to the Principal Investigator. Eligible candidates will be intimated through e-mail and called for interview at the Department of Biotechnology, NIT, Warangal. No TA/DA will be paid for attending the interview. Dr. K. Divakar (Principal Investigator) DST-INSPIRE Faculty Department of Biotechnology National Institute of Technology Warangal – 506 004. Telangana, India. E-mail: divakar@nitw.ac.in; kdivak@gmail.com

Advertisement: http://www.nitw.ac.in/nitw/announcements/2015/Notification_Project_Fellow_DST_INSPIRE_Biotechnology_NITW.pdf