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	<title><![CDATA[BOL: Related items]]></title>
	<link>https://bioinformaticsonline.com/related/28997?offset=1280</link>
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	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/41592/refka-a-fast-and-efficient-long-read-genome-assembly-approach-for-large-and-complex-genomes</guid>
	<pubDate>Fri, 01 May 2020 03:00:40 -0500</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/41592/refka-a-fast-and-efficient-long-read-genome-assembly-approach-for-large-and-complex-genomes</link>
	<title><![CDATA[RefKA: A fast and efficient long-read genome assembly approach for large and complex genomes]]></title>
	<description><![CDATA[<p><span>RefKA, a reference-based approach for long read genome assembly. This approach relies on breaking up a closely related reference genome into bins, aligning k-mers unique to each bin with PacBio reads, and then assembling each bin in parallel followed by a final bin-stitching step.</span></p>
<p>&nbsp;</p><p>Address of the bookmark: <a href="https://github.com/AppliedBioinformatics/RefKA" rel="nofollow">https://github.com/AppliedBioinformatics/RefKA</a></p>]]></description>
	<dc:creator>Rahul Nayak</dc:creator>
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	<guid isPermaLink="true">https://bioinformaticsonline.com/pages/view/9204/keep-your-important-ssh-session-running-when-you-disconnect-from-server</guid>
	<pubDate>Sat, 15 Mar 2014 21:39:17 -0500</pubDate>
	<link>https://bioinformaticsonline.com/pages/view/9204/keep-your-important-ssh-session-running-when-you-disconnect-from-server</link>
	<title><![CDATA[Keep Your Important SSH Session Running when You Disconnect from Server !!!]]></title>
	<description><![CDATA[<p>As a Bioinformatician/ Computational biologist we swim in the ocean of genomic/proteomics data, and play with them with an ease. In our day to day simulation, analysis, comparative study we do need to run exhaustive programs, which might take more than a week. In such cases we do need to disconnect from sever in a way that our program/session should not get terminated. To do so there are lots of software, tools such as tmux ( <a href="http://tmux.sourceforge.net/">http://tmux.sourceforge.net/</a>, nohup (<a href="http://ss64.com/bash/nohup.html">http://ss64.com/bash/nohup.html</a>) , byobu (<a href="https://help.ubuntu.com/10.04/serverguide/byobu.html">https://help.ubuntu.com/10.04/serverguide/byobu.html</a>) and other commands (disown -a &amp;&amp; exit), but following are the ones I use the most.</p><p>Screen is like a window manager for your console. It will allow you to keep multiple terminal sessions running and easily switch between them. It also protects you from disconnection, because the screen session doesn&rsquo;t end when you get disconnected.<br /><br />You&rsquo;ll need to make sure that screen is installed on the server you are connecting to. If that server is Ubuntu or Debian, just use this command:<br /><br />sudo apt-get install screen<br /><br />Now you can start a new screen session by just typing screen at the command line. You&rsquo;ll be shown some information about screen. Hit enter, and you&rsquo;ll be at a normal prompt.<br /><br /><strong>To disconnect (but leave the session running)</strong><br /><br />Hit Ctrl + A and then Ctrl + D in immediate succession. You will see the message [detached]<br /><br /><strong>To reconnect to an already running session</strong><br /><br />screen -r<br /><br /><strong>To reconnect to an existing session, or create a new one if none exists</strong><br /><br />screen -D -r<br /><br /><strong>To create a new window inside of a running screen session</strong><br /><br />Hit Ctrl + A and then C in immediate succession. You will see a new prompt.<br /><br /><strong>To switch from one screen window to another</strong><br /><br />Hit Ctrl + A and then Ctrl + A in immediate succession.<br /><br /><strong>To list open screen windows</strong><br /><br />Hit Ctrl + A and then W in immediate succession</p>]]></description>
	<dc:creator>Jitendra Narayan</dc:creator>
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	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/42267/hapsolo-an-optimization-approach-for-removing-secondary-haplotigs-during-diploid-genome-assembly-and-scaffolding</guid>
	<pubDate>Mon, 26 Oct 2020 21:23:36 -0500</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/42267/hapsolo-an-optimization-approach-for-removing-secondary-haplotigs-during-diploid-genome-assembly-and-scaffolding</link>
	<title><![CDATA[HapSolo: An optimization approach for removing secondary haplotigs during diploid genome assembly and scaffolding.]]></title>
	<description><![CDATA[<p><span>Despite marked recent improvements in long-read sequencing technology, the assembly of diploid genomes remains a difficult task. A major obstacle is distinguishing between alternative contigs that represent highly heterozygous regions. If primary and secondary contigs are not properly identified, the primary assembly will overrepresent both the size and complexity of the genome, which complicates downstream analysis such as scaffolding.</span></p>
<p><span>More at&nbsp;https://github.com/esolares/HapSolo</span></p><p>Address of the bookmark: <a href="https://github.com/esolares/HapSolo" rel="nofollow">https://github.com/esolares/HapSolo</a></p>]]></description>
	<dc:creator>Jit</dc:creator>
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	<guid isPermaLink="true">https://bioinformaticsonline.com/pages/view/9242/check-the-size-of-a-directory-free-disk-space</guid>
	<pubDate>Mon, 17 Mar 2014 02:35:32 -0500</pubDate>
	<link>https://bioinformaticsonline.com/pages/view/9242/check-the-size-of-a-directory-free-disk-space</link>
	<title><![CDATA[Check the Size of a directory &amp; Free disk space.]]></title>
	<description><![CDATA[<p>The amount of databases we bioinformatician deal are just HUGE &hellip; In such cases, we always need to check our server for free spaces etc. I planned this article to explains 2 simple commands that most bioinformatician want to know when they start using Linux / BioLinux. First: Size of a directory (du) and and second: free disk space that exists on your machine (df).</p><p><br /><strong>'du' &ndash; Check the size of a directory</strong></p><p><br />$ du<br />This command ( du) gives you a list of directories that exist in the current working directory along with their sizes in kilobytes (default). The last line of the output gives you the total size of the current directory including its subdirectories. <br /><br />$ du /home/jin1<br />The above command would give you the directory size of the directory /home/david<br /><br />$ du -h<br />The same &ldquo;du&rdquo;command with some flag gives you a better output than the default one. The option '-h' stands for human readable format. Therefore, in order to print the sizes of the files / directories in your desire notation use this time suffixed with a 'k' if its kilobytes and 'M' if its Megabytes and 'G' if its Gigabytes.<br /><br />$ du -ah<br />If you are interested in checking everything present in a folder use above mentioned command. It gives us not only the directories but also all the files that are present in the current directory. The &ldquo;-a&rdquo; flag displays the filenames along with the directory names in the output. <br /><br />$ du -c<br />This gives you a grand total as the last line of the output. So if your directory occupies 30MB the last 2 lines of the output would be 30M.<br /><br />$ du -s<br />Use this command to displays a summary of the directory size. It is the simplest way to know the total size of the current directory.<br /><br />$ du -S<br />This would display the size of the current directory excluding the size of the subdirectories that exist within that directory. So it basically shows you the total size of all the files that exist in the current directory.<br /><br />$ du --exculde=mp3<br />Several times it required to exclude some directory in our size calculation. In such cases the above command would display the size of the current directory along with all its subdirectories, but it would exclude all the files having the given pattern present in their filenames.</p><p><br /><strong>'df' - finding the disk free space / disk usage</strong><br /><br />$ df<br />Hmmm &hellip; now &ldquo;df&rdquo; command is really useful, and I guess you are going to use it over time. Typing the above command, outputs a table consisting of 6 columns. All the columns are very easy to understand. Remember that the 'Size', 'Used' and 'Avail' columns use kilobytes as the unit. The 'Use%' column shows the usage as a percentage which is also very useful.<br /><br />$ df -h<br />Displays the same output as the previous command but the '-h' indicates human readable format. Hence instead of kilobytes as the unit the output would have 'M' for Megabytes and 'G' for Gigabytes.<br /><br />Example: Linux installed on /dev/hda1<br />$ df -h | grep /dev/hda1</p><p><br />All right, this is not the only option to check the sizes and free spaces but there are a few more options that can be used with 'du' and 'df' . I will discuss it later.<br /><br /></p>]]></description>
	<dc:creator>Jitendra Narayan</dc:creator>
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	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/43057/hapsolo-an-optimization-approach-for-removing-secondary-haplotigs-during-diploid-genome-assembly-and-scaffolding</guid>
	<pubDate>Sat, 08 May 2021 21:25:00 -0500</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/43057/hapsolo-an-optimization-approach-for-removing-secondary-haplotigs-during-diploid-genome-assembly-and-scaffolding</link>
	<title><![CDATA[HapSolo: An optimization approach for removing secondary haplotigs during diploid genome assembly and scaffolding]]></title>
	<description><![CDATA[<p><span>HapSolo, that identifies secondary contigs and defines a primary assembly based on multiple pairwise contig alignment metrics. HapSolo evaluates candidate primary assemblies using BUSCO scores and then distinguishes among candidate assemblies using a cost function. The cost function can be defined by the user but by default considers the number of missing, duplicated and single BUSCO genes within the assembly. HapSolo performs hill climbing to minimize cost over thousands of candidate assemblies.&nbsp;</span></p><p>Address of the bookmark: <a href="https://github.com/esolares/HapSolo" rel="nofollow">https://github.com/esolares/HapSolo</a></p>]]></description>
	<dc:creator>Jit</dc:creator>
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  <guid isPermaLink='true'>https://bioinformaticsonline.com/opportunity/view/9441/jrf-at-gautam-buddha-university</guid>
  <pubDate>Thu, 27 Mar 2014 03:53:57 -0500</pubDate>
  <link></link>
  <title><![CDATA[JRF at Gautam Buddha University]]></title>
  <description><![CDATA[
<p>Gautam Buddha University (GBU) Noida invites applications for the follow posts<br />2014 March Advertisement from Gautam Buddha University (GBU)<br />Junior Research Fellow (JRF)<br />No. of Positions:  01<br />Educational Qualifications:<br />Master degree in any discipline of Life Science with NET qualified or valid GATE score. Desirable Qualification: Preference will be given to candidates having research experience in Bioinformatics<br />Experience:</p>

<p>(details of experience required)<br />Pay Scale:<br />INR Rs.12000/-P.M. + HRA<br />Category:<br />Science and Research Jobs<br />How To Apply:<br />The interested candidates should report for the Interview on 31st<br />March, 2014 at 10:00 am in the Conference Room of Dean, School of Biotechnology, First floor, Gautam Buddha University, Greater<br />Noida. Interested candidates may also send their resume to undersigned by post-mail/e-mail shaktis@gbu.ac.in or shaktisahi@gmail.com. No TA and DA will be paid for appearing for the interview<br />Download Official Notification:</p>

<p>http://www.gbu.ac.in/Recruitment/JRF_advertisement_DSTProject_Shakti_24March14.pdf</p>
]]></description>
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  <guid isPermaLink='true'>https://bioinformaticsonline.com/researchlabs/view/43293/josefa-gonzalez-lab</guid>
  <pubDate>Thu, 19 Aug 2021 08:52:56 -0500</pubDate>
  <link></link>
  <title><![CDATA[Josefa González Lab]]></title>
  <description><![CDATA[
<p>Lab focus on understanding how organisms adapt to their environments. They combine omics approaches with detailed molecular and phenotypic analyses to get a comprehensive picture of adaptation. Our aim at being internationally recognized as a leading lab in the field of environmental adaptation.<br />Lab share our passion for science with the general public by leading outreach projects aimed at increasing science awareness.</p>

<p>More at https://www.biologiaevolutiva.org/gonzalez_lab/</p>
]]></description>
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  <guid isPermaLink='true'>https://bioinformaticsonline.com/opportunity/view/9598/junior-research-fellowship-at-gb-pant-university</guid>
  <pubDate>Thu, 03 Apr 2014 12:29:46 -0500</pubDate>
  <link></link>
  <title><![CDATA[Junior Research Fellowship at G.B. PANT UNIVERSITY]]></title>
  <description><![CDATA[
<p>DEPARTMENT OF MOLECULAR BIOLOGY &amp; GENETIC ENGINEERING<br />COLLEGE OF BASIC SCIENCE AND HUMANITIES<br />G.B. PANT UNIVERSITY OF AGRICULTURE AND TECHNOLOGY<br />PANTNAGAR -263145, UTTARAKHAND</p>

<p>No. CBSH/MBGE/356</p>

<p>Subject: Advertisement for the award of Junior Research Fellowship.</p>

<p>Applications are invited for award of one Junior Research Fellowship on a consolidated fellowship of Rs. 12,000/- pm in the project “Bioinformatics Sub-DIC ”, under the Coordinatorship Dr. Anil Kumar. The fellowship is purely temporary and may continue till the duration of the project or maximum three years which ever is earlier. The appointment shall be given on six monthly review basis.</p>

<p>ESSENTIAL QUALIFICATION</p>

<p>M.Sc. Bioinformatics having research experience on In silico experimentation.</p>

<p>Candidates possessing the above qualifications may submit their application on<br />plain paper in the following format to the undersigned latest 18 April, 2014 the interviews will be held on 19 April, 2014 at 11.00 AM in the office of the undersigned. No separate letter for interview will be issued or any TA/DA will be paid for attending the interview.</p>

<p>Advertisement: http://www.gbpuat.ac.in/01042014_18april14_Advertisement%20for%20JRF%20Position,%20BI.pdf</p>
]]></description>
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<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/43661/maftools</guid>
	<pubDate>Fri, 17 Dec 2021 03:18:28 -0600</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/43661/maftools</link>
	<title><![CDATA[maftools]]></title>
	<description><![CDATA[<p>With advances in Cancer Genomics, <a href="https://docs.gdc.cancer.gov/Data/File_Formats/MAF_Format/">Mutation Annotation Format</a> (MAF) is being widely accepted and used to store somatic variants detected. <a href="http://cancergenome.nih.gov">The Cancer Genome Atlas</a> Project has sequenced over 30 different cancers with sample size of each cancer type being over 200. <a href="https://wiki.nci.nih.gov/display/TCGA/TCGA+MAF+Files">Resulting data</a> consisting of somatic variants are stored in the form of <a href="https://docs.gdc.cancer.gov/Data/File_Formats/MAF_Format/">Mutation Annotation Format</a>. This package attempts to summarize, analyze, annotate and visualize MAF files in an efficient manner from either TCGA sources or any in-house studies as long as the data is in MAF format.</p>
<p>https://www.bioconductor.org/packages/devel/bioc/vignettes/maftools/inst/doc/maftools.html</p><p>Address of the bookmark: <a href="https://github.com/PoisonAlien/maftools" rel="nofollow">https://github.com/PoisonAlien/maftools</a></p>]]></description>
	<dc:creator>Surabhi Chaudhary</dc:creator>
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  <guid isPermaLink='true'>https://bioinformaticsonline.com/opportunity/view/9859/bioinformatics-jrfsrf-position-at-university-of-hyderabad</guid>
  <pubDate>Tue, 15 Apr 2014 20:07:52 -0500</pubDate>
  <link></link>
  <title><![CDATA[Bioinformatics JRF/SRF position at University of Hyderabad]]></title>
  <description><![CDATA[
<p>UNIVERSITY OF HYDERABAD SCHOOL OF LIFE SCIENCES </p>

<p>Applications are invited from qualified individuals for a JRF/SRF position (sponsored by DBT/DST) at Prof. Jagan Pongubala’s laboratory, University of Hyderabad. Dr. Pongubala’s laboratory is investigating the molecular pathways that control the development of innate and adaptive immune cell types utilizing a combination of genetic, molecular and computational approaches.</p>

<p>JRF/SRF</p>

<p>Masters degree in Bioinformatics  (M.Sc./M.Tech.)</p>

<p>Rs. 12,000+HRA<br />Rs. 16,000+HRA</p>

<p>Initial appointment is for one year and  subjected to renewal up to 2 years</p>

<p>Candidates selected for the above position would have a choice to work on computational biology or experimental  biology. Candidates interested to work on computational biology are expected to perform high-throughput sequencing  (NGS) data analysis and should have a strong background in Bioinformatics &amp; Computational Biology, good  programming skills particularly Perl, Python, R and work experience in Linux environment.</p>

<p>Candidates interested to work on experimental biology should have work experience in techniques that are routinely  used in molecular biology and mammalian cell culture. A basic knowledge of bioinformatics is also desired. </p>

<p>Applicants for the above positions should have a Masters degree (M.Tech/M.Sc) with an aggregate marks greater  than 70% or a 7.5 CGPA. Candidates having JRF-fellowship through CSIR/UGC/ICMR/DBT will be encouraged  to enroll into Ph.D. program. The interested candidates having excellent organizational skills and the ability to work  in a team environment with an aspiration to learn new techniques and explore new scientific areas are requested to generate their resume using the link https://cvmkr.com/CV/new#0 and forward to pongubalajagan@gmail.com</p>

<p>Review of applications will begin immediately and continue until the position is filled. Eligible candidates will be called for an interview. No TA/ DA will be paid for attending the interview or at the time of joining the post. Applicants should note that the appointment is purely temporary and subjected to renewal up to three years and there is no Right to Claim for any regular appointment with the University.</p>

<p>Corresponding address: Jagan Pongubala, Ph.D.<br />Department of Animal Sciences<br />School of Life Sciences, Room:S44<br />University of Hyderabad<br />Gachibowli, Hyderabad 500046</p>

<p>Advertisement: https://www.uohyd.ac.in/images/recruitment/jrf-srf_130414.pdf</p>
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