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	<title><![CDATA[BOL: Related items]]></title>
	<link>https://bioinformaticsonline.com/related/29274?offset=1490</link>
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	<description><![CDATA[]]></description>
	
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	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/38441/genome-sequence-based-sub-species-delineation</guid>
	<pubDate>Wed, 12 Dec 2018 08:31:14 -0600</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/38441/genome-sequence-based-sub-species-delineation</link>
	<title><![CDATA[Genome sequence-based (sub-)species delineation.]]></title>
	<description><![CDATA[<p>The GGDC web service reports digital DDH for a universal and accurate delineation of prokaryotic (sub-)species without inheriting the pitfalls of classic DDH, and also calculates differences in genomic G+C content.</p>
<p>http://ggdc.dsmz.de/ggdc_background.php#</p>
<p><small>Genome-to-Genome Distance Calculator 2.1</small></p>
<p>http://ggdc.dsmz.de/ggdc.php</p>
<p>&nbsp;</p><p>Address of the bookmark: <a href="http://ggdc.dsmz.de/" rel="nofollow">http://ggdc.dsmz.de/</a></p>]]></description>
	<dc:creator>Abhimanyu Singh</dc:creator>
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  <guid isPermaLink='true'>https://bioinformaticsonline.com/opportunity/view/21065/ra-bioinformatics-at-north-eastern-hill-university</guid>
  <pubDate>Sat, 07 Feb 2015 06:06:05 -0600</pubDate>
  <link></link>
  <title><![CDATA[RA Bioinformatics at North Eastern Hill University]]></title>
  <description><![CDATA[
<p>Bioinformatics Infrastructure Facility, Department of RDAP, NEHU vacancy of Research Associate</p>

<p>Name of the Post: Research Associate<br />No. of the Post: 01 One<br />Age Limit: Max. 35 years<br />Salary: Rs. 22000/- per month plus HRA</p>

<p>Required Job Profile:<br />Candidate must possess M.Sc. in bioinformatics or biotechnology from recognized university or institute.<br />Desired Job Profile;<br />Candidate having Ph.D. or pursuing Ph.D. in the related subject or equivalent published work in reputed peer reviewed journals or advance PG dipoma in bioinformatics course.</p>

<p>How to apply:<br />Eligible and interested candidates should need to send the bio-data and bring all related documents in original and set of attested copies of the same in the time of interview.</p>

<p>Last date: 16.02.2015<br />Refer to http://www.nehu.ac.in/Advertisements/BIFTuraAdvt_221214.pdf</p>

<p>Summary <br />Employer Address:	Dr.B.K. Mishra Coordinator BIF, RDAP Department, North Eastern Hill University, Tura Campus, Tura, Meghalaya<br />Email:	drbkm1972@yahoo.co.in;birendramishra14@gmail.com<br />URL:	http://www.nehu.ac.in/Advertisements/BIFTuraAdvt_221214.pdf<br />Phone:	03651-223107<br />Required Skills:	not mentioned / required for this post<br />Required Experience:	not mentioned / required for this job post<br />Required Education:	M.Sc. in bioinformatics or biotechnology from recognized university or institute.<br />Job Location:	Tura, Meghalaya, India</p>
]]></description>
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	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/38758/roary-the-pan-genome-pipeline</guid>
	<pubDate>Tue, 22 Jan 2019 05:52:07 -0600</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/38758/roary-the-pan-genome-pipeline</link>
	<title><![CDATA[Roary: the Pan Genome Pipeline]]></title>
	<description><![CDATA[<p><span>Roary is a high speed stand alone pan genome pipeline, which takes annotated assemblies in GFF3 format (produced by Prokka (Seemann, 2014)) and calculates the pan genome. Using a standard desktop PC, it can analyse datasets with thousands of samples, something which is computationally infeasible with existing methods, without compromising the quality of the results. 128 samples can be analysed in under 1 hour using 1 GB of RAM and a single processor. To perform this analysis using existing methods would take weeks and hundreds of GB of RAM. Roary is not intended for meta-genomics or for comparing extremely diverse sets of genomes.</span></p><p>Address of the bookmark: <a href="https://sanger-pathogens.github.io/Roary/" rel="nofollow">https://sanger-pathogens.github.io/Roary/</a></p>]]></description>
	<dc:creator>Jit</dc:creator>
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	<guid isPermaLink="true">https://bioinformaticsonline.com/news/view/21241/pacman</guid>
	<pubDate>Mon, 16 Feb 2015 12:15:17 -0600</pubDate>
	<link>https://bioinformaticsonline.com/news/view/21241/pacman</link>
	<title><![CDATA[Pacman]]></title>
	<description><![CDATA[<p><span>The pacman package is an R package management tool that combines the functionality of base library related functions into intuitively named functions. This package is ideally added to .Rprofile to increase workflow by reducing time recalling obscurely named functions, reducing code and integrating functionality of base functions to simultaneously perform multiple actions.<br /><br />Function names in the pacman package follow the format of p_xxx where &lsquo;xxx&rsquo; is the task the function performs. For instance the p_load function allows the user to load one or more packages as a more generic substitute for the library or require functions and if the package isn&rsquo;t available locally it will install it for you.<br /><br /></span></p><p><strong>Installation</strong></p><p><span>To download the development version of pacman:</span></p><p><span>Download the </span><a href="https://github.com/trinker/pacman/zipball/master">zip ball</a><span> or </span><a href="https://github.com/trinker/pacman/tarball/master">tar ball</a><span>, decompress and run </span><code>R CMD INSTALL</code><span> on it, or use th</span><span>e </span><strong>devtools</strong><span> package to install the development version:</span></p><pre title="">## Make sure your current packages are up to date
update.packages()
## devtools is required
devtools::install_github("trinker/pacman")
</pre><p>Note: Windows users need <a href="http://www.murdoch-sutherland.com/Rtools/">Rtools</a> and <a href="http://CRAN.R-project.org/package=devtools">devtools</a> to install this way.</p><p>More at https://github.com/trinker/pacman</p><p>&nbsp;</p>]]></description>
	<dc:creator>Rahul Nayak</dc:creator>
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	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/39269/ragoo-fast-reference-guided-scaffolding-of-genome-assembly-contigs</guid>
	<pubDate>Wed, 17 Apr 2019 19:45:22 -0500</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/39269/ragoo-fast-reference-guided-scaffolding-of-genome-assembly-contigs</link>
	<title><![CDATA[RaGOO: Fast Reference-Guided Scaffolding of Genome Assembly Contigs]]></title>
	<description><![CDATA[<p>Alonge M, Soyk S, Ramakrishnan S, Wang X, Goodwin S, Sedlazeck FJ, Lippman ZB, Schatz MC:&nbsp;<a href="https://www.biorxiv.org/content/early/2019/01/13/519637">Fast and accurate reference-guided scaffolding of draft genomes</a>.&nbsp;<em>bioRxiv</em>&nbsp;2019.</p>
<p>RaGOO is a tool for coalescing genome assembly contigs into pseudochromosomes via minimap2 alignments to a closely related reference genome. The focus of this tool is on practicality and therefore has the following features:</p>
<ol>
<li>Good performance. On a MacBook Pro using Arabidopsis data, pseudochromosome construction takes less than a minute and the whole pipeline with SV calling takes ~2 minutes.</li>
<li>Intact ordering and orienting of contigs.</li>
<li><a href="https://github.com/malonge/RaGOO/wiki/Breaking-Chimeric-Contigs">Chimeric contig correction</a></li>
<li><a href="https://github.com/malonge/RaGOO/wiki/GFF-File-Lift-Over">GFF lift-over</a></li>
<li><a href="https://github.com/malonge/RaGOO/wiki/Calling-Structural-Variants">Structural variant calling with and integrated version of Assemblytics</a></li>
<li>Confidence scores associated with the grouping, localization, and orientation for each contig.</li>
</ol><p>Address of the bookmark: <a href="https://github.com/malonge/RaGOO" rel="nofollow">https://github.com/malonge/RaGOO</a></p>]]></description>
	<dc:creator>BioJoker</dc:creator>
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  <guid isPermaLink='true'>https://bioinformaticsonline.com/opportunity/view/21435/ra-walk-in-interview-nbfgr-lucknow</guid>
  <pubDate>Tue, 24 Feb 2015 08:23:48 -0600</pubDate>
  <link></link>
  <title><![CDATA[RA WALK-IN-INTERVIEW @ NBFGR, Lucknow]]></title>
  <description><![CDATA[
<p>F.No. 1(122)/2015-Admn. (CABin Project)<br />Research Associate/Young Professional/SRF Zoology job vacancies in National Bureau of Fish Genetic Resources (NBFGR)<br />Post Name: Research Associate (Computer Science/ Applications)                <br />Qualification: Ph.D. In Computer Science/Computer Applications or equivalent. Or Post-Graduation in Computer Science/ Computer Applications with 1st Division or 60% marks or equivalent overall grade point average with at least two years of research experience. Desirable: 1. Expertise and experience of working/ handling High Performance Computing (H PC) and genomic resource data. 2. Expertise on database management, data mining technologies/ softwares/tools. 3. Published Research papers	<br />No.of Post: 1<br />Pay Scale: Consolidated Rs.24,000/- p.m. + HRA (as admissible) for Ph.D. holders and consolidated `23,000/- + HRA (as admissible) for Master degree holder.	<br />Age:40 years</p>

<p>Young Professional II (Computer Science/Applications)	<br />Master degree in Computer Science/Computer Applications/B.Tech (Computer Science) or equivalent. <br />Desirable: 1. Knowledge of Statistical and Computational Genomics/ Proteomics/ Bioinformatics/Data mining tools. 2. Experience in handling HPC, programming languages and database management packages.	<br />A consolidated salary of Rs.25,000/- per month.	<br />21 to 45 year</p>

<p>Young Professional II (Biotechnology/ Bioinformatics)	<br />Master degree in Bioinformatics/ Biotechnology/ B. Tech(Biotech) or equivalent. Desirable: 1. Knowledge of Computational Genomics/Proteomics/Bioinformatics. 2. Expertise in NGS data analysis and knowledge of allied software and tools.	<br />A consolidated salary of Rs.25,000/- per month.	</p>

<p>Senior Research Fellow	<br />1. Bachelors degree with Zoology, Fisheries and 2. Master's degree in Fishery science/ Zoology with Fisheries/ Biotechnology/ Life Sciences with specialization in Fisheries/ Molecular Biology. 3. 1 st Division or 60% marks or equivalent overall grade point average. <br />Desirable: Work experience in Fisheries, molecular research techniques, bioinformatics and Computer skills. NET qualified <br />Note: The project involves extensive exploration tours and sampling from water bodies all over India	<br />Rs.16,000/- p.m. for 1st &amp; 2nd year and `18,000/- p.m. for 3rd and subsequent years +HRA (as per rules)	35 years for male and 40 years for female candidate</p>

<p>How to apply</p>

<p>A walk-in-interview will be held on 04th March, 2015 at 10:00 hrs at National Bureau of Fish Genetic Resources, Lucknow. Eligible and desirous candidates fulfilling all the requirements may appear for the interview with duly filled in application giving full details of academic records and experience(s) along with attested photocopy as well as original copy of the relevant documents and a passport size photograph on the attached proforma.</p>

<p>http://www.nbfgr.res.in/</p>
]]></description>
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	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/40549/mgse-mapping-based-genome-size-estimation</guid>
	<pubDate>Fri, 17 Jan 2020 02:11:43 -0600</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/40549/mgse-mapping-based-genome-size-estimation</link>
	<title><![CDATA[MGSE: Mapping-based Genome Size Estimation]]></title>
	<description><![CDATA[<p>MGSE can harness the power of files generated in genome sequencing projects to predict the genome size. Required are the FASTA file containing a high continuity assembly and a BAM file with all available reads mapped to this assembly. The script construct_cov_file.py (https://doi.org/10.1186/s12864-018-5360-z) allows the generation of a COV file based on the (sorted) BAM file (also possible via MGSE directly). Next, this COV file can be used by MGSE to calculate the coverage in provided reference regions and to calculate the total number of mapped bases. Both values are subjected to the genome size estimation. Providing accurate reference regions is crucial for this genome size estimation.</p><p>Address of the bookmark: <a href="https://github.com/bpucker/MGSE" rel="nofollow">https://github.com/bpucker/MGSE</a></p>]]></description>
	<dc:creator>Shruti Paniwala</dc:creator>
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	<guid isPermaLink="true">https://bioinformaticsonline.com/pages/view/21443/a-guide-for-complete-r-beginners-getting-data-into-r</guid>
	<pubDate>Tue, 24 Feb 2015 20:15:08 -0600</pubDate>
	<link>https://bioinformaticsonline.com/pages/view/21443/a-guide-for-complete-r-beginners-getting-data-into-r</link>
	<title><![CDATA[A guide for complete R beginners :- Getting data into R]]></title>
	<description><![CDATA[<p>For a beginner this can be is the hardest part, it is also the most important to get right.</p><p>It is possible to create a vector by typing data directly into R using the combine function &lsquo;c&rsquo;</p><blockquote><p><strong>x </strong></p></blockquote><p>same as</p><blockquote><p><strong>x </strong></p></blockquote><p>creates the vector x with the numbers between 1 and 5.</p><p>You can see what is in an object at any time by typing its name;</p><blockquote><p><strong>x</strong></p></blockquote><p>will produce the output<strong> &lsquo;[1] 1 2 3 4 5&prime;</strong></p><p>Note that names need to be quoted</p><blockquote><p><strong>daysofweek </strong><strong>&larr; c(&lsquo;Monday&rsquo;, &lsquo;Tuesday&rsquo;, &lsquo;Wednesday&rsquo;, &lsquo;Thursday&rsquo;, &lsquo;Friday&rsquo;);</strong></p></blockquote><p>Usually however you want to input from a file. We have touched on the &lsquo;read.table&rsquo; function already.</p><blockquote><p><strong>mydata </strong></p></blockquote><p>Now <strong>mydata</strong> is a data frame with multiple vectors</p><p>each vector can be identified by the default syntax</p><p>#if any of these are typed it will print to screen</p><blockquote><p><strong>mydata$V1 mydata$V2 mydata$V3 </strong></p></blockquote><p>By default the function assumes certain things from the file</p><ul>
<li>The file is a plain text file (there are function to read excel files: <em>not covered here</em>)</li>
<li>columns are separated by any number of tabs or spaces</li>
<li>there is the same number of data points in each column</li>
<li>there is no header row (labels for the columns)</li>
<li>there is no column with names for the rows** [I&rsquo;ll explain].</li>
</ul><p><span style="text-decoration: underline;">If any of these are false, we need to tell that to the function</span></p><p>If it has a header column</p><blockquote><p><strong>mydata <em>header=T also works</em></strong></p></blockquote><p>Note that there is a comma between different parts of the functions arguments</p><p>If there is one less column in the header row, then R assumes that the 1<sup>st</sup> column of data after the header are the row names</p><p>Now the vectors (columns) are identified by their name</p><p>#if any of these are typed it will print to screen</p><blockquote><p><strong>mydata$A mydata$B mydata$C </strong></p></blockquote><p># Summary about the whole data frame</p><blockquote><p><strong>summary(mydata)</strong></p></blockquote><p># Summary information of column A</p><blockquote><p><strong>summary(mydata$A) </strong></p></blockquote><p>We can shortcut having to type the data frame each time by attaching it</p><blockquote><p><strong>attach(mydata)</strong></p></blockquote><p># summary of column B as &lsquo;mydata&rsquo; is attached</p><blockquote><p><strong>summary(B)</strong></p></blockquote><p><span style="text-decoration: underline;">Two other important options for </span><em><span style="text-decoration: underline;">read.table</span></em></p><p>If is is separated only by tabs and has a header</p><blockquote><p><strong>mydata </strong></p></blockquote><p>Really useful if you have spaces in the contents of some columns, so R does not mess up reading the columns . However if the columns or of an uneven length it will tell you.</p><p>If you know that the file has uneven columns</p><blockquote><p><strong>mydata </strong></p></blockquote><p>This causes R to fill empty spaces in a columns with &lsquo;NA&rsquo; .</p><p>The last two examples will still work with our file and give the same result as with only headers=T</p><p><span style="text-decoration: underline;">Graphs</span></p><p>to get an idea of what R is capable of type</p><blockquote><p><strong>demo(graphics)</strong></p></blockquote><p>steps through the examples, and the code is printed to the screen</p><p>We will work with simpler examples that have immediate use to biologists.</p><p>Remember to get more information about the options to a function type &lsquo;?function&rsquo;</p><p><span style="text-decoration: underline;">Histogram of A</span><span style="text-decoration: underline;"></span></p><blockquote><p><strong>hist(mydata$A)</strong></p></blockquote><p>If there was more data we could increase the number of vertical columns with the option, breaks=50 (or another relevant number).</p><blockquote><p><strong>boxplot(mydata)</strong></p></blockquote><p>We can get rid of the need to type the data frame each time by using the <strong>attach</strong> function</p><p># if not already done so</p><blockquote><p><strong>attach(mydata) </strong></p><p><strong>boxplot(mydata$A, mydata$B, name=c(&ldquo;Value A&rdquo;, &ldquo;Value B&rdquo;) , ylab=&ldquo;Count of Something&rdquo;)</strong></p></blockquote><p>same as</p><blockquote><p><strong>boxplot(A, B, name=c(&ldquo;Value A&rdquo;, &ldquo;Value B&rdquo;) , ylab=&ldquo;Count of Something&rdquo;)</strong></p></blockquote><p><span style="text-decoration: underline;">Scatter plot</span></p><p># if not already done so</p><blockquote><p><strong>attach(mydata) </strong></p><p><strong>plot(A,B) # or plot(mydata$A, mydata$B)</strong></p></blockquote><p><strong><span style="text-decoration: underline;">SAVING an image</span></strong></p><p>Windows users (Rgui) RIGHT click on image and select which you want.</p><p><span style="text-decoration: underline;">These instructions work for everyone.</span></p><p>You need to create a new device of the type of file you need, then send the data to that device</p><p>to save as a png file (easy to load into the likes of powerpoint, also great for web applications.</p><blockquote><p><strong>png(&lsquo;filename&rsquo;) </strong></p><p><strong>boxplot(A, B, name=c(&ldquo;Value A&rdquo;, &ldquo;Value B&rdquo;) , ylab=&ldquo;Count of Something&rdquo;)</strong></p></blockquote><p>or to save as a pdf</p><blockquote><p><strong>pdf(&lsquo;filename&rsquo;) </strong></p><p><strong>boxplot(A, B, name=c(&ldquo;Value A&rdquo;, &ldquo;Value B&rdquo;) , ylab=&ldquo;Count of Something&rdquo;)</strong></p></blockquote><p><span style="text-decoration: underline;">Note</span></p><ul>
<li>Nothing will appear on screen, the output is going to the file</li>
<li>Also it may not be saved immediately but will once the device (or R) is turned quit.</li>
</ul><p>To quit R type</p><p><strong>q() # </strong>If you save your session, next time you start R, you will have your data preloaded.</p><p>Or if you want to remain in R</p><blockquote><pre><strong>dev.off() #</strong>turns of the png (or pdf etc) device, thus forces the data to save</pre></blockquote>]]></description>
	<dc:creator>Archana Malhotra</dc:creator>
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	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/41330/u-plot-genome-u-plot-sample-implementation</guid>
	<pubDate>Tue, 03 Mar 2020 01:39:12 -0600</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/41330/u-plot-genome-u-plot-sample-implementation</link>
	<title><![CDATA[U-Plot: Genome U-Plot sample implementation]]></title>
	<description><![CDATA[<p>The Genome U-Plot is a JavaScript tool to visualize Chromosomal abnormalities in the Human Genome using a U-shape layout.</p>
<p><img src="https://raw.githubusercontent.com/gaitat/GenomeUPlot/master/public/data/LNCAP.png" alt="image" style="border: 0px;"></p><p>Address of the bookmark: <a href="https://github.com/gaitat/GenomeUPlot" rel="nofollow">https://github.com/gaitat/GenomeUPlot</a></p>]]></description>
	<dc:creator>Rahul Nayak</dc:creator>
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  <guid isPermaLink='true'>https://bioinformaticsonline.com/opportunity/view/21539/research-associate-at-central-potato-research-institute-cpri-shimla-himachal-pradesh</guid>
  <pubDate>Wed, 11 Mar 2015 03:07:37 -0500</pubDate>
  <link></link>
  <title><![CDATA[RESEARCH ASSOCIATE at Central Potato Research Institute (CPRI) - Shimla, Himachal Pradesh]]></title>
  <description><![CDATA[
<p>One post of Research Associate for Project Implementation Unit in the time bound project “XII Plan -–Centre of Agricultural Bio-informatics(CABIN)” are to be filled on purely contractual basis which will be co-terminus with the project as per the details given as under : </p>

<p>No of post : 01 <br />Essential qualifications: i) Ph. D degree in Bioinformatics/computers/Bio-technology. OR ii) Master’s Degree in Bioinformatics/computers/Bio-technology with 1st division or 60% marks or equivalent overall grade point average with at least two years of research experience as evidenced from fellowship/Associateship/training/other engagements. <br />Desirable qualifications: i) Working Knowledge and Published Research papers in Bio-informatics. <br />Monthly emoluments : Rs. 23,000/- + HRA . for M.Sc degree holder Rs. 24,000/- + HRA for Ph.D degree holder <br />Maximum Age limit : Research Associate – Males- 40 years &amp; Women 45 years. <br />SELECTION PROCEDURE FOR CENTRAL POTATO RESEARCH INSTITUTE (CPRI) – RESEARCH ASSOCIATE POST: </p>

<p>Written Test on 20/03/2015. <br />Shortlisted candidates will undertake face to face interview. <br />Dates are yet to be announced for the final selection <br />WALK-IN PROCEDURE FOR RESEARCH ASSOCIATE VACANCY IN CENTRAL POTATO RESEARCH INSTITUTE (CPRI): </p>

<p>Interested/eligible candidates should submit their application along with the attested copies of educational qualification (provisional degree of Masters and Ph.D is mandatory )/experience certificates and one passport size photograph to the Asstt. Admn. Officer(E-I), CPRI, Shimla-171001 at 9.30 AM on the date of interview. The candidates appearing for interview must bring original certificate with them and only those candidates possessing essential qualification as per advertisement will be interviewed. The Director, CPRI, Shimla reserves the right either to fill up the post or cancel the interview without assigning any reasons thereof. Application form is available in the website ( website: http//cpri.ernet.in). No TA/DA will be given by the Institute to the candidates. The Institute is located at Bemloe which is about 2 Kms from Main Bus Stand(Old)/3 Kms. from the Railway Station and about 5 Kms. from ISBT (Tutikandi).</p>
]]></description>
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