BOL: Related items

Common steps for reads mapping !

BioStar — Thu, 09 Mar 2023 02:48:02 -0600

Mapping reads to a reference genome is an essential step in many types of genomic analysis, such as variant calling and gene expression analysis. Here are some general steps to follow for mapping reads to a genome:

Choose a read mapper: There are many read mappers available, such as BWA, Bowtie, and HISAT2. Choose a mapper that is appropriate for your type of data and research question.
Index the reference genome: Before mapping reads, the reference genome needs to be indexed. This involves creating an index of the genome sequence that allows the mapper to quickly find matches to the reads. Most mappers have their own indexing tools.
Prepare the read data: The reads should be in a format that is compatible with the mapper. Most mappers accept FASTQ or BAM files. Depending on the quality of the data, it may need to be filtered or trimmed before mapping.
Run the mapper: The mapper is run with the command-line interface or using a graphical user interface. The specific command depends on the mapper being used, but typically involves specifying the input data, reference genome, and output file format.
Evaluate the mapping results: After the mapping is complete, the results should be evaluated. This includes assessing the quality of the mapping, such as the mapping rate, the number of mapped reads, and the mapping quality score.
Post-processing: Depending on the analysis being performed, post-processing of the mapped reads may be necessary. This can include filtering reads based on quality, removing duplicate reads, and calling variants.

Overall, mapping reads to a reference genome is a complex process that requires careful consideration of the type of data, the research question, and the specific mapper being used.

Bioinformatics Training Fellowship !!

Fri, 28 Aug 2015 16:02:25 -0500

Applications are invited from suitable candidates for six months ‘Training Fellowship’ in Bioinformatics under the BTISNET program of DBT in the Distributed Information Sub center (DISC) facility at NIPGR, New Delhi, under the supervision of Dr. Gitanjali Yadav, Scientist, NIPGR.

Project Title No. of post(s) Designation Stipend in Tenure Start End
Establishment of Distributed Information Sub-Centre (DISC) 02 Trainee Fellow / Student Fellow 5,000/- p.m Six months 01/01/2016 30/06/2016

Essential Qualification:

Traineeship: Candidate having B.Tech or Master Degree or equivalent in Bioinformatics/ Biotechnology with strong interest in Computational Biology and First class/ division throughout academic career may apply.

Studentship: Students currently pursuing the final year of B.Tech or Masters Degree or equivalent in Bioinformatics/ Biotechnology, requiring a thesis as a necessary pre-requisite for completion of respective degree and First class/ division throughout academic career may apply.

Desirable Qualification: Proficiency in Coding Algorithms and Bioinformatics Applications, evidenced by short trainings or computing courses.

The positions are purely temporary and co-terminus with the tenure of the training period as mentioned above. NIPGR reserves the right to select the candidate against the above fellowship depending upon the qualification and experience of the candidate. Reservations shall be as per Govt. of India norms. The applicants will have no claim implicit or explicit for consideration against any regular position of DISC/NIPGR.

Eligible candidates may apply online application form available

at http://www.nipgr.res.in/discform.html within 15 days from the date of

advertisement.Applications received through any other mode will be disqualified outright.

More at http://www.nipgr.res.in/discform.html

List of bioinformatics workflow management tools !

Rahul Nayak — Sat, 20 Mar 2021 00:15:25 -0500

Here are list of Workflow Managers

BigDataScript – A cross-system scripting language for working with big data pipelines in computer systems of different sizes and capabilities. [ paper-2014 | web ]
Bpipe – A small language for defining pipeline stages and linking them together to make pipelines. [ web ]
Common Workflow Language – a specification for describing analysis workflows and tools that are portable and scalable across a variety of software and hardware environments, from workstations to cluster, cloud, and high performance computing (HPC) environments. [ web ]
Cromwell – A Workflow Management System geared towards scientific workflows. [ web ]
Galaxy – a popular open-source, web-based platform for data intensive biomedical research. Has several features, from data analysis to workflow management to visualization tools. [ paper-2018 | web ]
Nextflow (recommended) – A fluent DSL modelled around the UNIX pipe concept, that simplifies writing parallel and scalable pipelines in a portable manner. [ paper-2018 | web ]
Ruffus – Computation Pipeline library for python widely used in science and bioinformatics. [ paper-2010 | web ]
SeqWare – Hadoop Oozie-based workflow system focused on genomics data analysis in cloud environments. [ paper-2010 | web ]
Snakemake – A workflow management system in Python that aims to reduce the complexity of creating workflows by providing a fast and comfortable execution environment. [ paper-2018 | web ]
Workflow Descriptor Language – Workflow standard developed by the Broad. [ web ]

Pacasus: Correction of palindromes in long reads from PacBio and Nanopore

BioStar — Mon, 12 Nov 2018 05:26:48 -0600

Tool for detecting and cleaning PacBio / Nanopore long reads after whole genome amplification. Check the poster from the Revolutionizing Next-Generation Sequencing (2nd edition) conference in the source folder: https://github.com/swarris/Pacasus/blob/master/vib2017.pdf.

The prepint version is found on http://www.biorxiv.org/content/early/2017/08/09/173872

It uses the pyPaSWAS framework for sequence alignment (https://github.com/swarris/pyPaSWAS)

Address of the bookmark: https://github.com/swarris/Pacasus

RA Bioinformatics at University of Delhi

Thu, 10 Sep 2015 16:02:37 -0500

BIOINFORMATICS INFRASTRUCTURE FACILITY

GARGI COLLEGE (University of Delhi)

SiriFort Road, New Delhi-110049

Walk- in- interview Bioinformatics Infrastructure Facility (BIF), Gargi College, University of Delhi invites to appear for interview on 29th September, 2015 at 9.30 AM for filling up the following purely temporary position sponsored by DBT, New Delhi.

1. Traineeship – 01 (one post) purely temporary for a period of six months.

Salary: Rs.8000/- p.m. fixed.

Essential Qualification: Post Graduate degree in Bioinformatics or any other branch of Life Sciences preferably with dissertation in Bioinformatics.

Desirable Qualification: Prior knowledge of programming languages such as C, VB, SQL etc. and software/database development.

2. Research Associate-01(one post) purely temporary for a period of nine months.

Salary: Rs 36000/-+HRA p.m fixed.

Essential Qualification: PhD in Bioinformatics/Biological Sciences/Computer Science or allied sciences with proven experience in bioinformatics.

3. Studentship- 01 (one post) purely temporary for a period of six months.

Salary: Rs.8000/- p.m. fixed.

Essential Qualifications: Final year Post Graduate students pursuing a degree in Bioinformatics or any branch of Life Science with knowledge of bioinformatics.

Interested candidates are required to appear for the walk in interview on 29th September, 2015 at 9.30 AM in Principal’s Office, Gargi College, Sirifort Road, N. Delhi-110049, with their CVs, original documents and a set of Photostat copies of all original documents. Conditions: The original documents must be produced at the time of interview, otherwise will not be allowed to attend the same. No TA & DA will be paid for appearing in the interview. The institute reserves the right to fill or not to fill the positions depending upon qualifications/credentials of the candidates etc. The appointment does not confer any right over the job and will not be considered as institute’s service. Dr Aparajita Mohanty Dr Shashi Tyagi (Co-coordinator,BIF) (Coordinator, BIF)

http://gargi.du.ac.in/uploads/ngrey/News/Gargi_Advt_BIF_2015.pdf

ngs-bits - Short-read sequencing tools

Neel — Thu, 16 Jan 2020 23:14:00 -0600

Binaries of ngs-bits are available via Bioconda. Alternatively, ngs-bits can be built from sources:

Binaries for Linux/macOS
From sources for Linux/macOS
From sources for Windows

Address of the bookmark: https://github.com/imgag/ngs-bits

National Research Centre on Plant Biotechnology for RA/JRF positions

Sat, 26 Sep 2015 20:32:49 -0500

National Research Centre on Plant Biotechnology - New Delhi, Delhi
National Research Centre on Plant Biotechnology recruitments job vacancies for RA/JRF positions

Name of post: Research Associate
Salary: Rs.36000/-+ 30% HRA*
Educational Qualification: Candidates should have PhD in Biotechnology / Bioinformatics Life Sciences (or) M.Sc Biotechnology / Bioinformatics with three years research experience in relevant field

Name of post: Junior Research Fellow
Salary: Rs.25000/-+ 30% HRA*
Educational Qualification: Candidates should have M.Sc in Biotechnology /Bioinformatics / Life Sciences with 1st Division or 60% marks or equivalent overall grade point from any recognized professional University

Age Limit: 35 years max. (5 years relaxation for SC/ST/OBC)

How to attend walk in interview?
Interested candidates may attend Walk- in-interview on 1st October, 2015 at 10 am at NRCPB, LBS Building, Pusa Campus, and New Delhi-110012 at the above address along with updated Bio-data (CV), ID Proof & attested copies of Certificates and Original to prove qualification & Experience.

Recruitment reference: http://www.nrcpb.org/jobslist

simNGS and simLibrary – Software for Simulating Next-Gen Sequencing Data

Jit — Tue, 28 Nov 2017 06:49:11 -0600

simNGS is software for simulating observations from Illumina sequencing machines using the statistical models behind the AYB base-calling software. By default, observations only incorporate noise due to sequencing and do not incorporate effects from more esoteric sources of noise that may be present in real data ("dust", bubbles, merged clusters, sequence-heterogeneous clusters, etc). Many of these additional sources may optionally applied.

simNGS takes fasta format sequences and a file describing the covariance of noise between bases and cycles observed in an actual run of the machine, randomly generates noisy intensities representing the signals for the sequence at each cycle and calculates likelihoods for all possible base calls.

Address of the bookmark: https://www.ebi.ac.uk/goldman-srv/simNGS/

sim3C: Read-pair simulation of 3C-based sequencing methodologies (HiC, Meta3C, DNase-HiC)

Jit — Tue, 13 Nov 2018 07:25:38 -0600

Required python modules

biopython
intervaltree
numpy
scipy
tqdm
PyYAML

Address of the bookmark: https://github.com/cerebis/sim3C

Bioinformatics Faculty at UNIVERSITY OF HYDERABAD

Wed, 14 Oct 2015 22:53:44 -0500

UNIVERSITY OF HYDERABAD

(A Central University established by an Act of Parliament)

Prof. C.R.Rao Road, P.O. Central University Campus, Gachibowli,

Hyderabad - 500 046

Advt.No. UH/HR/Rectt-2015/02 dt. 12.10.2015

The University invites applications from the Indian citizens for the following positions:

Professor / Associate Professor / Assistant Professor :

Biotechnology & Bioinformatics

Last date : 16th November 2015

More Info : http://www.uohyd.ac.in/images/recruitment/advt-121015.pdf