BOL: Related items

HairSplitter: assembling long reads in an unknown number of haplotypes

BioStar — Wed, 07 Dec 2022 00:13:40 -0600

Pros and cons of HairSplitter Limitations of HairSplitter:

Not very fast: it re-polishes the whole assembly

Limited in the number of haplotypes

Strengths of HairSplitter:

Very modular, can be used with any assembler

Naive: makes no assumption on ploidy, parameter-free

Safe: won’t artificially duplicate contigs

HairSplitter splits collapsed assemblies from “draft” assemblies obtained by any means

HairSplitter can recover haplotypes and distinguish repeated elements

Only needs sequencing reads, potentially error-prone

HairSplitter splits collapsed assemblies from “draft” assemblies obtained by any means

HairSplitter can recover haplotypes and distinguish repeated elements

Only needs sequencing reads, potentially error-prone

Not really available yet (github.com/RolandFaure/HairSplitter)

https://hal.archives-ouvertes.fr/hal-03864075/file/RolandFaure_presentation_SeqBIM_2022.pdf

Address of the bookmark: https://hal.archives-ouvertes.fr/hal-03817928/document

SRF/JRF Bioinformatics at CIARI

Fri, 04 Dec 2015 00:10:09 -0600

Realizing the importance of Island Agriculture to meet the requirements of local population and tourists, Indian Council of Agricultural research (ICAR) established Central Island Agricultural Research Institute, Port Blair on June 23rd, 1978 by merging different regional research stations of ICAR institutes located in Islands. The ultimate aim of CIARI is the developments of island agricultural production technologies which utilizes the strengths of the island and convert the constraints in opportunities, without causing any ill effect to the fragile ecosystem of the island.The institute has made tremendous progress in the Agriculture development of the islands during the last three decades. Keeping in view the natural resources of the islands diversity, fragile ecosystem, research program would be designed to maximize the productivity without disturbing to the islands ecosystem to provide better and decent livelihood and as a source of revenue and resource generation. Research and development in Agriculture sector should cover all disciplines in order to have a balanced progress in all disciplines for the overall benefits of the farmers of these islands.

Position I

Job Title : Junior Research Fellow

No. of Posts : One

Project : Establishment of sub distributed information centre

Qualification : M.Sc in Basic Science with NET or B.Sc in professional course with NET or M.Sc in professional course

Desired Experience : Experience in Bioinformatics and molecular biology

Payscale : Rs. 25000 per month

Age Limit : Upto 35 for men and 40 for women with 5 years relaxation to SC/ST and 3 years relaxation for OBC.

Position II

Job Title : Traineeship

No. of Posts : One

Project : Establishment of sub distributed information centre

Qualification : B.Sc Bioinformatics /Biotechnology / Life Science / Computer Science

Desired Experience : Experience in Bioinformatics and molecular biology

Payscale : Rs. 8000 per month

Age Limit : Upto 35 for men and 40 for women with 5 years relaxation to SC/ST and 3 years relaxation for OBC.

Position III

Job Title : Studentship

No. of Posts : Two

Project : Establishment of sub distributed information centre

Qualification : B.Sc Bioinformatics /Biotechnology / Life Science / Computer Science

Desired Experience : Experience in Bioinformatics and molecular biology

Payscale : Rs. 8000 per month

Age Limit : Upto 35 for men and 40 for women with 5 years relaxation to SC/ST and 3 years relaxation for OBC.

How to Apply : Candidates who meet the requirements can attend the walk in interview at CIARI,Port Blair on 09.12.2015 10.30AM.

http://icar-ciari.res.in/employment/9-12-15.pdf

Jabba: Hybrid Error Correction for Long Sequencing Reads

Jit — Fri, 05 Jan 2018 03:58:14 -0600

Jabba is a hybrid error correction tool to correct third generation (PacBio / ONT) sequencing data, using second generation (Illumina) data.

Input

Jabba takes as input a concatenated de Bruijn graph and a set of sequences:

the de Bruijn graph should appear in fasta format with 1 entry per node, the meta information should be in the format:
>NODE
the set of sequences should be in fasta or fastq format. These sequences will be corrected (e.g. PacBio reads). The corrections will be written to a file Jabba fasta.
The output is a file in fasta format with corrections of the long reads, and additionally a file in the input format containing uncorrected reads.

https://github.com/biointec/jabba/wiki

https://almob.biomedcentral.com/articles/10.1186/s13015-016-0075-7

Address of the bookmark: https://github.com/biointec/jabba

Post-doc position at LABGeM - Evry, France

Fri, 11 Dec 2015 06:24:00 -0600

The LABGeM team (CEA/Genoscope, CNRS UMR 8030, France, Dir. Claudine Médigue) is developing integrated approaches which combines bioinformatics methods based (i) on genomic and metabolic contexts, (ii) on an orignal metabolic network representation and (iii) on a structural classification of active sites for the discovery of new metabolic enzymatic activities.

We are hiring a post-doctoral fellow for the development of innovative bioinformatics methods to explore metabolic networks and enzyme families. These methods will be based on protein family analysis and graph approaches combining genomic and metabolic contexts.

For more details, please see this link : http://goo.gl/tHQOqk

Qualifications:
PhD degree in bioinformatics or computational biology
- Previous experience in network or protein family analysis
- Programming skills (C/C++, Python, Java) and in common biostatistical analyses
- Team player, innovative and creative thinking, good oral and written communication skills

24 months, Post Doctoral position
Start: from March 2016
Place: CEA, Genoscope UMR8030, LABGeM (Laboratory of Bioinformatics Analyses for Genomics and Metabolism), Evry, France
Contact: David Vallenet, vallenet@genoscope.cns.fr
Publications: https://scholar.google.com/citations?user=rJNPLSAAAAAJ
Remuneration per month: from 2,850 €

Interested candidates should send their CV, statement of research interests, and contact information of at least 2 references to David Vallenet (vallenet@genoscope.cns.fr).

Consed--A Finishing Package (BAM File Viewer, Assembly Editor, Autofinish, Autoreport, Autoedit, and Align Reads To Reference Sequence)

Neel — Fri, 07 Feb 2020 07:16:22 -0600

Supports Illumina, 454, other Next-Gen and Sanger Reads and allows mixtures of these read types
Consed includes BamScape which can view bam files with unlimited numbers of reads. BamScape can bring up consed to edit reads and the reference sequence in targeted regions.
Consed is compatible with Newbler, Cross_match, Phrap, MIRA, Velvet and PCAP output.
Quickly takes the user to each variant site for viewing (also available as an automated report)
Overview of assembly can help detect and fix misassemblies
Editing time reduced by the program's ability to pin-point problem areas
Editing is guided by error probabilities

Address of the bookmark: http://www.phrap.org/consed/consed.html

Microscope

Jitendra Narayan — Fri, 04 Mar 2016 05:26:31 -0600

Microscope Platform user documentation.

The MicroScope platform is available at this URL:

https://www.genoscope.cns.fr/agc/microscope

Address of the bookmark: http://microscope.readthedocs.org/en/latest/index.html

PostDoc at IISER -TVM

Fri, 26 Feb 2016 03:53:05 -0600

Postdoctoral Fellowship/ Research Associateship

Eligibility : M Phil / Phd

Location : Thiruvananthapuram

Last Date : 30 Apr 2016

Hiring Process : Face to Face Interview
IISER -TVM

The Postdoctoral Fellowship/Research Associateship is a full-time, contractual position for highly qualified young scientists to carry out research at CCMS, IISER-TVM.

Research areas at the Centre

Quantum Chemistry/ Computational Fluid Dynamics/Condensed Matter Physics (Theory)/Genomics/Genetics/Gravitational Waves

Qualifications: PhD in Bioinformatics / Biophysics / Physics / Astrophysics / Chemistry / Mathematics / Engineering (Mechanical/Aerospace) Those who are in the final stages of their Ph.D. thesis submission are also eligible to apply. However, those candidates must have submitted the thesis at the time of the interview.

Experience: Applicants should have at least three peer reviewed publications and relevant experience in the research area they are applying for.

No. of positions: 5

Age limit: 35 yrs or below. A relaxation of 5 yrs will be applicable to candidates belonging to SC/ST/OBC and women candidates

Salary: The Fellowship carries a remuneration of INR Rs. 5,18,000 - Rs. 5,76,000 per annum (including HRA). The postdoctoral fellowship may not be held concurrently with any other national or international fellowships. It is also not transferable to any other fellowship
How to apply

Applications should reach the Head, CCMS, IISER Thiruvananthapuram,CET Campus, Engineering College PO,Thiruvananthapuram 695016 on or before April 30, 2016 by e-mail to ccms@iisertvm.ac.in by mentioning the research area name in the subject line.

More at http://www.iisertvm.ac.in/openings/read_opening/150.phpx

SvABA: Structural variation and indel detection by local assembly

Jit — Tue, 10 Mar 2020 07:52:15 -0500

SvABA is a method for detecting structural variants in sequencing data using genome-wide local assembly. Under the hood, SvABA uses a custom implementation of SGA (String Graph Assembler) by Jared Simpson, and BWA-MEM by Heng Li. Contigs are assembled for every 25kb window (with some small overlap) for every region in the genome. The default is to use only clipped, discordant, unmapped and indel reads, although this can be customized to any set of reads at the command line using VariantBam rules. These contigs are then immediately aligned to the reference with BWA-MEM and parsed to identify variants. Sequencing reads are then realigned to the contigs with BWA-MEM, and variants are scored by their read support.

Address of the bookmark: https://github.com/walaj/svaba

Project Assistant at IISER Mohali

Fri, 29 Jan 2016 11:04:27 -0600

Project Assistant Job position in Indian Institute of Science Education & Research (IISER) Mohali

Title : In silico understanding of molecular basis of recognition, binding, and regulation of mRNA by STAR family of transcriptional regulators.

No. of Post : 01

Department : Science and Technology

Qualifications : M.Sc./B.Tech in computational life sciences, computational chemistry, computational natural sciences or allied areas. Working experience in MD simulations, bioinformatics, molecular modeling, and drug designing is desirable and plus

Emoluments : As per DST norms
How to apply

Applicants are requested to send application along with bio-data and a summary of previous projects (if any) as a PDF file with the e-mail to Dr. Monika Sharma, Email: mnsharma@iisermohali.ac.in. Last date of applications is 17:00 IST. Feb 15, 2016. Shortlisted candidates will be called for interview on Feb 22, 2016.

More at http://14.139.227.202/tenders/tenderinvite/index.php/iiserm-project-openings/554-applications-are-invited-to-work-as-project-assistant-in-a-dst-inspire-research-project-funded-by-department-of-science-and-technology-india

flo

Jitendra Narayan — Wed, 10 Feb 2016 10:52:32 -0600

flo - same species annotations lift over pipeline

Lift over is the process of transferring annotations from one genome assembly to another. Usually lift over is done because there is a new, improved genome assembly for the species and good quality annotations (maybe manually curated or experimentally verified) are available on the old assembly.

The idea is simple: align the new assembly with the old one (e.g., with BLAT), process the alignment data to define how a coordinate or coordinate range on the old assembly should be transformed to the new assembly (e.g., as a chain file), transform the coordinates (e.g., with liftOver).

https://github.com/wurmlab/flo

Address of the bookmark: https://github.com/wurmlab/flo