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	<title><![CDATA[BOL: Related items]]></title>
	<link>https://bioinformaticsonline.com/related/30124?offset=680</link>
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	<guid isPermaLink="true">https://bioinformaticsonline.com/blog/view/32587/ten-international-scholarships-for-indian-biotechnology-and-bioinformatics-students</guid>
	<pubDate>Wed, 10 May 2017 04:51:02 -0500</pubDate>
	<link>https://bioinformaticsonline.com/blog/view/32587/ten-international-scholarships-for-indian-biotechnology-and-bioinformatics-students</link>
	<title><![CDATA[Ten International Scholarships for Indian Biotechnology and Bioinformatics Students]]></title>
	<description><![CDATA[<p>Wherever you go around the world, Indian students are in demand. With countries such as Canada and Australia providing huge incentives to Indian students to lure them to their shores, there are many institutions around the world that offer scholarships exclusively to Indian students. Historically these scholarships tend to be aimed towards Masters and PhD programmes however on the rise are scholarships for undergraduate students. Student World Online takes a look at ten international scholarships for Indian undergraduate students abroad.</p><p><br /><span><strong>1.&nbsp;</strong></span><strong><a href="http://admissions.cornell.edu/apply/international-students/tata-scholarship"><span>TATA SCHOLARSHIP</span></a></strong>&nbsp;- Cornell University, New York State, USA<br />Tata, the Indian multinational conglomerate company, have a foundation known as the Tata Education &amp; Development Trust which has&nbsp;<span style="text-decoration: underline;"><a href="http://www.news.cornell.edu/stories/2008/10/tata-trust-gives-50-million-endowment-cornell" target="_blank">endowed a multi million dollar sum to Cornell University</a></span>&nbsp;to provide undergraduate scholarships to 20 Indian students every year. &nbsp;In another example of supporting American universities, the Tata group also pledged US$50 million to Harvard University in recent years, whose executive management programme&nbsp;<span style="text-decoration: underline;"><a href="http://en.wikipedia.org/wiki/Ratan_Tata" target="_blank">Ratan Tata</a></span>&nbsp;attended in the 1970s. &nbsp;<a href="http://admissions.cornell.edu/apply/international-students/tata-scholarship" target="_blank"><span><span style="text-decoration: underline;">Read more...&nbsp;</span></span></a>&nbsp;<br /><br /><strong><span>2.</span></strong>&nbsp;<a href="http://www.uow.edu.au/future/international/apply/scholarships/UOW135799.html" target="_blank"><strong><span>BRADMAN FOUNDATION SCHOLARSHIP</span></strong></a>&nbsp;- University of Wollongong, Australia.<br />Named after Australia's cricket legend&nbsp;<span style="text-decoration: underline;"><a href="http://en.wikipedia.org/wiki/Donald_Bradman" target="_blank">Donald Bradman</a></span>, the&nbsp;<span style="text-decoration: underline;"><a href="https://www.uow.edu.au/content/groups/public/@web/@unia/documents/doc/uow145334.pdf" target="_blank">UOW Bradman Foundation Scholarship</a></span>&nbsp;was launched in 2012, with the help of Adam Gilchrist no less, to offer one successful Indian student each year a 50% reduction in tuition fees. &nbsp;<a href="http://www.uow.edu.au/future/international/apply/scholarships/UOW135799.html" target="_blank"><span><span style="text-decoration: underline;">Read more...</span></span></a>&nbsp;&nbsp;</p><p><span><strong>3.&nbsp;</strong></span><strong><a href="http://www.huaweischolarships.org/about_scholar.aspx" target="_blank"><span>HUAWEI MAITREE SCHOLARSHIPS</span></a></strong>&nbsp;- Various Universities, China<br />Along with Tata, Huawei are the other huge corporation to be featured. &nbsp;China's massive telecoms equipment vendor are involved in these scholarships offered to Indian students studying in China. &nbsp;In 2013 there are 10 generous scholarships available which provide full tuition fees and living expenses. &nbsp;The courses on which the scholarships are offered include Science and Technology courses, Social Sciences and Culture and Development courses. &nbsp;<a href="http://www.huaweischolarships.org/about_scholar.aspx" target="_blank"><span><span style="text-decoration: underline;">Read more...</span></span></a></p><p><span><strong>4.&nbsp;</strong></span><strong><a href="http://www.britishcouncil.in/study-uk/dr-manmohan-singh-scholarships-2013" target="_blank"><span>DR. MANMOHAN SINGH SCHOLARSHIPS</span></a></strong>&nbsp;- Cambridge University, England, UK<br />These scholarships have been designed to help budding Indian minds follow in the footsteps of&nbsp;<span style="text-decoration: underline;"><a href="http://pmindia.nic.in/" target="_blank">Indian prime minister Manmohan Singh</a></span>&nbsp;by studying at the prestigious Cambridge University. &nbsp;The scholarships can be applied to any undergarduate course (with the two exceptions of medicine and veterinary science) and cover everything, i.e. tuition and college fees, living expenses and an additional grant to go towards travel expenses. &nbsp;<a href="http://www.britishcouncil.in/study-uk/dr-manmohan-singh-scholarships-2013" target="_blank"><span><span style="text-decoration: underline;">Read more...</span></span></a><br /><br /><span><strong>5.&nbsp;</strong></span><strong><a href="http://www.oxbridgeindia.com/scholarship.php"><span>OXFORD AND CAMBRIDGE SOCIETY OF INDIA</span></a></strong>&nbsp;- Oxford &amp; Cambridge Universities, England, UK<br />As the name might suggest, these are scholarships available for students wishing to study at Oxford or Cambridge (cleverly known together as&nbsp;<span style="text-decoration: underline;"><a href="http://en.wikipedia.org/wiki/Oxbridge" target="_blank">Oxbridge</a></span>). &nbsp;It is only available for applicants who are completing or have completed a degree at an Indian university, however these scholarships are for both undergraduate and graduate students.&nbsp;&nbsp;<a href="http://www.oxbridgeindia.com/scholarship.php" target="_blank"><span><span style="text-decoration: underline;">Read more...</span></span></a></p><p><span><strong>6.&nbsp;</strong></span><strong><a href="http://www.napier.ac.uk/study/international/funding/Pages/india-scholarships.aspx" target="_blank"><span>EDINBURGH NAPIER UNIVERSITY</span></a></strong>&nbsp;- Scotland, UK<br />This one applies to all countries in the Indian subcontinent and is for both undergraduate and graduate courses. Edinburgh Napier University offers a merit based discount of &pound;2,000 Pounds. &nbsp;<a href="http://www.napier.ac.uk/study/international/funding/Pages/india-scholarships.aspx" target="_blank"><span><span style="text-decoration: underline;">Read more...</span></span></a></p><p><span><strong>7.&nbsp;</strong></span><strong><a href="http://www.sheffield.ac.uk/international/countries/asia/south-asia/india/scholarships" target="_blank"><span>SHEFFIELD UNIVERSITY</span></a></strong>&nbsp;- Sheffield, UK<br />Provides merit-based scholarships for undergraduate and graduate programmes across all subjects<span>.</span>&nbsp;<a href="http://www.sheffield.ac.uk/international/countries/asia/south-asia/india/scholarships" target="_blank"><span><span style="text-decoration: underline;">Read more...</span></span></a><br /><br /><span><strong>8.&nbsp;</strong></span><strong><a href="http://www.india4eu.eu/scholarships" target="_blank"><span>INDIA 4EU II</span></a></strong>&nbsp;- Several Universities across Europe<br />Pioneered by the European Union and involving partner universities in France, Finland, Germany, Italy, Portugal, Spain and Sweden,&nbsp;<span style="text-decoration: underline;"><a href="http://www.india4eu.eu/" target="_blank">the India 4EU II initiative</a></span>&nbsp;is aimed at encouraging Indian students to study, work and live in Europe. &nbsp;The initiative is well funded and allows the successful students tuition fees, expenses for living and travel costs as well as insurance during their time at one of the partner universities. &nbsp;<a href="http://www.india4eu.eu/scholarships" target="_blank"><span><span style="text-decoration: underline;">Read more...</span></span></a><br /><br /><span><strong>9.&nbsp;</strong></span><strong><a href="http://www.tcd.ie/international/Indian%20Scholarship.php" target="_blank"><span>TRINITY COLLEGE DUBLIN</span></a></strong>&nbsp;- Ireland<br />Valid for undergraduate courses in the faculties of Arts, Humanities, Social Sciences, Science, Computer Science or Engineering, the Trinity College Dublin offers Indian students scholarships to the tune of&nbsp;&euro;9,000 per annum over a year degree course. &nbsp;<a href="http://www.tcd.ie/international/Indian%20Scholarship.php" target="_blank"><span><span style="text-decoration: underline;">Read more...</span></span></a><br /><br /><span><strong>10.&nbsp;</strong></span><strong><a href="http://www.indianexpress.com/news/university-college-dublin-announces--euro-250000-scholarship-for-indian-students/1094390/" target="_blank"><span>UNIVERSITY COLLEGE DUBLIN</span></a></strong>&nbsp;- Ireland<br />Another of Ireland and Dublin's finest, the UCD awards one Global Excellence Undergraduate Scholarship which provides the worthy student a substantial 50% towards their tuition fees and is valid for all courses save medicine, radiography and veterinary medicine. &nbsp;UCD also offers a Global Undergraduate Scholarship scheme for undergrads accepted on science, social sciences, arts and business courses. &nbsp;This is all thanks to a &euro;250,000 fund that will allow for 57 Indian students to benefit from scholarships at UCD. &nbsp;<a href="http://www.indianexpress.com/news/university-college-dublin-announces--euro-250000-scholarship-for-indian-students/1094390/"><span><span style="text-decoration: underline;">Read more...</span></span></a></p>]]></description>
	<dc:creator>Priya Singh</dc:creator>
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	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/32726/ergo-20-bioinformatics-suites</guid>
	<pubDate>Tue, 16 May 2017 08:14:10 -0500</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/32726/ergo-20-bioinformatics-suites</link>
	<title><![CDATA[ERGO 2.0 Bioinformatics suites]]></title>
	<description><![CDATA[<p>ERGO 2.0 provides a systems biology informatics toolkit centered on comparative genomics to capture, query, and visualize sequenced genomes. &nbsp;Using Igenbio's proprietary algorithms, and the most comprehensive genomic database integrated with the largest collection of microbial metabolic and non-metabolic pathways, ERGO&trade; assigns functions to genes, integrates genes into pathways, and identifies previously unknown or mischaracterized genes, cryptic pathways, and gene products.&nbsp;</p><p>Address of the bookmark: <a href="https://www.igenbio.com/ergo/" rel="nofollow">https://www.igenbio.com/ergo/</a></p>]]></description>
	<dc:creator>Jit</dc:creator>
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  <guid isPermaLink='true'>https://bioinformaticsonline.com/opportunity/view/33794/senior-bioinformatics-software-developer-hyderabad-telangana</guid>
  <pubDate>Mon, 03 Jul 2017 10:10:31 -0500</pubDate>
  <link></link>
  <title><![CDATA[Senior Bioinformatics Software Developer, Hyderabad, Telangana]]></title>
  <description><![CDATA[
<p>DuPont Pioneer is the world leader in plant biotechnology area including discovery, development and delivery of elite crop genetics. DuPont Pioneer is aggressively building Big Data and Predictive Analytics capabilities in order to deliver improved services to our customers. We are currently seeking Senior Bioinformatics Software Developer at the DuPont Knowledge Center in Hyderabad, India for our global Data Science and Informatics group. At DuPont Pioneer, you’ll become part of a work environment that nurtures your interests, ignites your passion, creates opportunities to serve and helps you attain success–both personally and professionally. The hiring level will be commensurate with the level of experience. This is a critical position with the potential to make immediate, significant impact on our business.<br />The successful candidate will have an extensive background in computer science and bioinformatics through courses or academic degrees, and proven experience in bioinformatics software development. We are looking for those creative, smart, model driven, agile individuals who enjoy giving their all to tackle diverse software needs.<br />Duties / Responsibilities</p>

<p>Job Qualifications<br />Education and Experience<br />•	Master Degree in Bioinformatics, Computational biology, Scientific Computing or related field <br />•	3-5 years of Post-Master’s experience in Bioinformatics software development <br />•	Proven experience developing high throughput bioinformatics applications<br />Required Competencies<br />•	Strong proven experience in Python programming language in Linux environment<br />•	Proven High Performance computing experience (LSF/SGE/OGE)<br />•	Exposure in code versioning and repository management (GIT/SVN)<br />•	Proven experience in Bioinformatics algorithm development<br />•	Deep understanding in Bioinformatics tools, data types<br />Desired Competencies<br />•	Familiarity working in a scientific computing environment (NumPy, SciPy, Pandas etc.)<br />•	Familiarity working with Cloud technologies (AWS, Azure)<br />•	Ability to demonstrate solid analytical skills and exceptional attention to detail.<br />•	Experience in relational databases and data structures<br />•	Proven experience working with teams using agile software development methodologies and processes<br />•	Familiarity with Service Oriented Architecture (SOA)<br />•	Familiarity with build tools (Jenkins, make, ANT, Maven)<br />•	Exposure to project management tools (JIRA, Confluence, RED MINE, etc.)</p>

<p>More at http://careers.dupont.com/jobsearch/job-details/senior-bioinformatics-software-developer/012939W-01/</p>
]]></description>
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	<guid isPermaLink="true">https://bioinformaticsonline.com/news/view/34375/the-10th-north-east-bioinformatics-network-nebinet-annual-coordinators-meet</guid>
	<pubDate>Sat, 18 Nov 2017 15:02:44 -0600</pubDate>
	<link>https://bioinformaticsonline.com/news/view/34375/the-10th-north-east-bioinformatics-network-nebinet-annual-coordinators-meet</link>
	<title><![CDATA[The 10th North East Bioinformatics Network (NEBINet) Annual Coordinators' Meet]]></title>
	<description><![CDATA[<p>The 10th North East Bioinformatics Network (NEBINet) Annual Coordinators' Meet organised by the Bioinformatics Centre, St Edmund's College, Shillong and sponsored by the Department of Biotechnology, Government of India, was held at St Edmund's College Auditorium here on Thursday. Meghalaya Governor Ganga Prasad graced the inaugural programme as chief guest. <br />In his inaugural address, the Governor said the panorama of scientific scenario has greatly changed over the years, the thrust areas have undergone a metamorphosis but the conceptual underpinning of the basic sciences still continues. <br />"Of late, the activity of basic research has been intricately intertwined with technology. And we are determined to carry forward this change, for it is through technology that science can actually reach the masses in our country and afar, and the changing times have also inculcated a culture of cross-departmental and interdisciplinary research. Science and technology has always played a pivotal role in taking a nation towards greater heights by ways of innovations and inventions," he added. <br />Prasad also hoped that discussions, suggestions and sharing of innovative ideas during the two-day 10th NEBINet Annual Coordinators' Meet will open up new avenues to make substantial advancement in Biological Sciences which will provide a platform for proper and effective delivery mechanism for the common man. <br />During the inaugural function, Advisor of Department of Biotechnology Dr T Madhan Mohan gave an overview of the NEBINet and Bioinformatics programme. <br />President of Epygen Biotech FZ LLC, Dubai, UAE, Dr Debayan Ghosh, delivered the keynote address. <br />St Edmund's College governing body secretary Brother Simon Coelho and St Edmund's College Principal Dr Sylvanus Lamare also spoke during the function.</p>]]></description>
	<dc:creator>Jit</dc:creator>
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	<guid isPermaLink="true">https://bioinformaticsonline.com/blog/view/34916/bioinformatics-tools-developed-for-oxford-nanopore-data-analysis</guid>
	<pubDate>Wed, 27 Dec 2017 20:47:30 -0600</pubDate>
	<link>https://bioinformaticsonline.com/blog/view/34916/bioinformatics-tools-developed-for-oxford-nanopore-data-analysis</link>
	<title><![CDATA[Bioinformatics tools developed for Oxford Nanopore data analysis !]]></title>
	<description><![CDATA[<p><span>MinION is the only portable real-time device for DNA and RNA&nbsp;</span><span>sequencing</span><span>. Each consumable flow cell can now generate 10&ndash;20 Gb of DNA&nbsp;</span><span>sequence</span><span>&nbsp;data. Ultra-</span><span>long read lengths are possible (hundreds of kb) as you can choose your fragment length.&nbsp;</span>One of the technical advantages of ONT data is the read length, which offers great prospects for genome assembly. Generally, assemblers are based on several different types of algorithms, such as greedy, overlap-layout-consensus (OLC), de Bruijn graph (DBG), and string graph.</p><p><span>List of analysis tools developed for Oxford Nanopore data</span></p><p>BWA <br />Fast nanopore data tuned alignment tool <br />https://github.com/lh3/bwa</p><p>GraphMap<br />Mapper for long and error-prone reads<br />https://github.com/isovic/graphmap</p><p>LAST<br />Nanopore tuned alignment tool<br />http://last.cbrc.jp/</p><p>LINKS<br />Software tool for long read scaffolding <br />https://github.com/warrenlr/LINKS/</p><p>marginAlign<br />Tools to align nanopore reads to a reference<br />https://github.com/benedictpaten/marginAlign</p><p>minoTour<br />Real time analysis tools<br />http://minotour.nottingham.ac.uk/</p><p>nanoCORR<br />Error-correction tool for nanopore sequence data<br />https://github.com/jgurtowski/nanocorr</p><p>NanoOK<br />Software for nanopore data, quality and error profiles<br />https://documentation.tgac.ac.uk/display/NANOOK/NanoOK</p><p>Nanopolish<br />Nanopore analysis and genome assembly software<br />https://github.com/jts/nanopolish</p><p>nanopore<br />Variant-detection tool for nanopore sequence data<br />https://github.com/mitenjain/nanopore</p><p>Nanocorrect<br />Error-correction tool for nanopore sequence data<br />https://github.com/jts/nanocorrect/</p><p>npReader<br />Real-time conversion and analysis of nanopore reads<br />https://github.com/mdcao/npReader</p><p>poRe<br />Tool for analyzing and visualizing nanopore data<br />https://sourceforge.net/p/rpore/wiki/Home/</p><p>PoreSeq<br />Error-correction and variant-calling software<br />https://github.com/tszalay/poreseq</p><p>Poretools<br />Nanopore sequence analysis and visualization software <br />https://github.com/arq5x/poretools</p><p>SSPACE-LongRead<br />Genome scaffolding tool <br />http://www.baseclear.com/genomics/bioinformatics/basetools/SSPACE-longread</p><p>SMIS<br />Genome scaffolding tool <br />https://sourceforge.net/projects/phusion2/files/smis/</p><p>&nbsp;</p><p>List of assemblers for Oxford Nanopore MinION long reads</p><p>LQS<br />DALIGNER, Celera OLC Nanocorrect, <br />Nanopolish corrector<br />https://github.com/jts/nanopolish</p><p>PBcR<br />HGAP or BLASR, Celera OLC <br />PBcR corrector<br />http://wgs-assembler.sourceforge.net/wiki/index.php/PBcR<br /> &ndash;<br />Canu<br />MHAP, Celera OLC <br />Canu corrector<br />https://github.com/marbl/canu</p><p>Falcon<br />String graph, Celera OLC <br />Falcon corrector<br />https://github.com/PacificBiosciences/falcon</p><p>Miniasm <br />OLC<br />https://github.com/lh3/miniasm</p><p>ra-integrate<br />OLC<br />https://github.com/mariokostelac/ra-integrate/</p><p>ALLPATHS-LG<br />de Bruijn graph <br />ALLPATHS-L corrector<br />https://www.broadinstitute.org/software/allpaths-lg/blog/?page_id=12</p><p>SPAdes <br />de Bruijn graph <br />SPAdes corrector<br />http://bioinf.spbau.ru/spades</p>]]></description>
	<dc:creator>biogeek</dc:creator>
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	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/38804/grabb-selective-assembly-of-genomic-regions-a-new-niche-for-genomic-research</guid>
	<pubDate>Sat, 26 Jan 2019 18:58:16 -0600</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/38804/grabb-selective-assembly-of-genomic-regions-a-new-niche-for-genomic-research</link>
	<title><![CDATA[GRAbB: Selective Assembly of Genomic Regions, a New Niche for Genomic Research]]></title>
	<description><![CDATA[<p><span>GRAbB is shown to be more efficient than MITObim in terms of speed, memory and disk usage. The other functionalities (handling multiple targets simultaneously and extracting homologous regions) of the new program are not matched by other programs. The program is available with explanatory documentation at&nbsp;</span><a href="https://github.com/b-brankovics/grabb">https://github.com/b-brankovics/grabb</a><span>. GRAbB has been tested on Ubuntu (12.04 and 14.04), Fedora (23), CentOS (7.1.1503) and Mac OS X (10.7). Furthermore, GRAbB is available as a docker repository: brankovics/grabb (</span><a href="https://hub.docker.com/r/brankovics/grabb/">https://hub.docker.com/r/brankovics/grabb/</a><span>).</span></p><p>Address of the bookmark: <a href="https://github.com/b-brankovics/grabb" rel="nofollow">https://github.com/b-brankovics/grabb</a></p>]]></description>
	<dc:creator>Rahul Nayak</dc:creator>
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  <guid isPermaLink='true'>https://bioinformaticsonline.com/researchlabs/view/35552/the-brent-lab</guid>
  <pubDate>Fri, 09 Feb 2018 10:55:27 -0600</pubDate>
  <link></link>
  <title><![CDATA[The Brent Lab]]></title>
  <description><![CDATA[
<p>The Brent Lab is developing and applying computational methods for mapping gene regulation networks, modeling them quantitatively, and engineering new behaviors into them.</p>
]]></description>
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	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/40940/consed-a-finishing-package-bam-file-viewer-assembly-editor-autofinish-autoreport-autoedit-and-align-reads-to-reference-sequence</guid>
	<pubDate>Fri, 07 Feb 2020 07:16:22 -0600</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/40940/consed-a-finishing-package-bam-file-viewer-assembly-editor-autofinish-autoreport-autoedit-and-align-reads-to-reference-sequence</link>
	<title><![CDATA[Consed--A Finishing Package (BAM File Viewer, Assembly Editor, Autofinish, Autoreport, Autoedit, and Align Reads To Reference Sequence)]]></title>
	<description><![CDATA[<ul>
<li>Supports Illumina, 454, other Next-Gen and Sanger Reads and allows mixtures of these read types</li>
<li>Consed includes BamScape which can view bam files with unlimited numbers of reads. BamScape can bring up consed to edit reads and the reference sequence in targeted regions.</li>
<li>Consed is compatible with Newbler, Cross_match, Phrap, MIRA, Velvet and PCAP output.</li>
<li>Quickly takes the user to each variant site for viewing (also available as an automated report)</li>
<li>Overview of assembly can help detect and fix misassemblies</li>
<li>Editing time reduced by the program's ability to pin-point problem areas</li>
<li>Editing is guided by error probabilities</li>
</ul><p>Address of the bookmark: <a href="http://www.phrap.org/consed/consed.html" rel="nofollow">http://www.phrap.org/consed/consed.html</a></p>]]></description>
	<dc:creator>Neel</dc:creator>
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	<guid isPermaLink="true">https://bioinformaticsonline.com/pages/view/36197/bioinformatics-oneliner</guid>
	<pubDate>Tue, 10 Apr 2018 04:13:03 -0500</pubDate>
	<link>https://bioinformaticsonline.com/pages/view/36197/bioinformatics-oneliner</link>
	<title><![CDATA[Bioinformatics OneLiner]]></title>
	<description><![CDATA[<p>To remove all line ends (\n) from a Unix text file:</p><pre>sed ':a;N;$!ba;s/\n//g' filename.txt &gt; newfilename_oneline.txt</pre><p>To get average for a column of numbers (here the second column $2):</p><pre>awk '{ sum += $2; n++ } END { if (n &gt; 0) print sum / n; }'</pre><p>To get sequence length for all sequences in a fasta file:</p><pre>awk '/^&gt;/ {if (seqlen){print seqlen}; print ;seqlen=0;next; } { seqlen = seqlen +length($0)}END{print seqlen}' \<br />filename.fasta</pre><p>To copy (move, rename, etc) files based on their list in a text file:</p><pre>cat file_list.txt | while read line; do cp "$line" complete_dataset/"$line"; done</pre><p>To split bam files into sets with mapped and unmapped reads:</p><pre>samtools view -F4 sample.bam &gt; sample.mapped.sam<br />samtools view -f4 sample.bam &gt; sample.unmapped.sam</pre><p>To gzip all your fastq files using gnu parallel and gzip:</p><pre>parallel gzip ::: *.fastq</pre><p>To gzip all your fastq files using pigz:</p><pre>pigz *.fastq</pre><p>To count all sequences in a fasta file:</p><pre>grep "^&gt;" yourfile.fasta -c</pre><p>To count all sequences in all fasta files in your current directory:</p><pre>for a in *.fasta; do ls $a; grep "^&gt;" -c $a; done</pre><p>To keep only one copy of duplicated lines:</p><pre>awk '!seen[$0]++'</pre><p>To sum assembly size from SPAdes contigs.fasta or scaffolds.fasta file:</p><pre>grep "^&gt;" scaffolds.fasta | cut -f 4 -d '_' | paste -sd+ | bc</pre><p>To remove everything after the first space at each line, e.g. to to simplify fasta headers:</p><pre>cut -d' ' -f1 &lt; your_file</pre><p>To count reads in a all .fastq.gz files in your current folder (fast, using gnu parallel):</p><pre>parallel "echo {} &amp;&amp; gunzip -c {} | wc -l | awk '{d=\$1; print d/4;}'" ::: *.gz</pre><p>To count reads in a all .fastq.gz files in your current folder:</p><pre>zcat *.gz | echo $((`wc -l`/4))</pre><p>To count reads in a all .fastq files in your current folder:</p><pre>cat *.fastq | echo $((`wc -l`/4))</pre><p>To count base pairs in a all .fastq.gz files in your current folder:</p><pre>zcat *.fastq.gz | paste - - - - | cut -f 2 | tr -d '\n' | wc -c </pre><p>To split multifasta file into many fasta files:</p><pre>awk '/^&gt;/ {OUT=substr($0,2) ".fa"}; {print &gt;&gt; OUT; close(OUT)}' Input_File</pre><p>To convert Illumina FASTQ 1.3 to 1.8:</p><pre>sed -e '4~4y/@ABCDEFGHIJKLMNOPQRSTUVWXYZ[\\]^_`abcdefghi/!"#$%&amp;'\''()*+,-.\/0123456789:;&lt;=&gt;?@ABCDEFGHIJ/' f.fastq</pre><p>To convert FASTQ to FASTA:</p><pre>sed -n '1~4s/^@/&gt;/p;2~4p' </pre><p>To get fastq read length distribution:</p><pre>cat reads.fastq | awk '{if(NR%4==2) print length($1)}' | sort | uniq -c</pre><p>To deinterleave interleaved fastq file:</p><pre>cat myf.fq | paste - - - - - - - - | tee &gt;(cut -f 1-4 | tr "\t" "\n" &gt; myfile_1.fq) | cut -f 5-8 | \<br />tr "\t" "\n" &gt; myf2.fq </pre><p>To filter and sort contig identifiers from SPAdes assembly (e.g. here lenght &gt;= 4000 + coverage &gt;=100):</p><pre>grep "^&gt;" scaffolds.fasta | sed s"/_/ /"g | awk '{ if ($4 &gt;= 4000 &amp;&amp; $6 &gt;= 100) print $0 }' | sort -k 4 -n | \<br />sed s"/ /_/"g</pre><p>To append something to all headers of your fasta files:</p><pre>sed 's/&gt;.*/&amp;YOURSTRING/' filename.fasta &gt; new_filename.fasta</pre><p>To replace/squeeze multiple adjacent spaces by only one space:&nbsp;</p><pre>tr -s " " &lt; file</pre><p>To filter fastq based on length (here larger than or equal to 21, but smaller than or equal to 25.</p><pre>cat your.fastq | paste - - - - | awk 'length($2)&nbsp; &gt;= 21 &amp;&amp; length($2) &lt;= 25' | sed 's/\t/\n/g' &gt; filtered.fastq</pre><p>To print difference between the last and first row in 5th column:</p><pre>awk '{if (!first){first=$5;}; last=$5;} END {print last-first}' myfile.txt</pre><p>To sample only 200 first bases from all sequences in a multifasta file (e.g. from assembly scaffolds.fasta file here):</p><pre>awk '/^&gt;/{ seqlen=0; print; next; } seqlen &lt; 200 { if (seqlen + length($0) &gt; 200) $0 = substr($0, 1, 200-seqlen);\<br /> seqlen += length($0); print }' scaffolds.fasta &gt; 200bp_scaffolds.fasta</pre><p>&nbsp;To pipe a compressed fasta file directly into makeblastdb.</p><pre>gunzip -c fasta.gz | makeblastdb -in -</pre><p>To remove sequences with duplicate fasta headers from a fasta file.</p><pre>awk '/^&gt;/{f=!d[$1];d[$1]=1}f' in.fasta &gt; out.fasta</pre>]]></description>
	<dc:creator>Rahul Nayak</dc:creator>
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	<guid isPermaLink="true">https://bioinformaticsonline.com/pages/view/36384/binding-site-prediction-in-protein</guid>
	<pubDate>Wed, 25 Apr 2018 04:35:57 -0500</pubDate>
	<link>https://bioinformaticsonline.com/pages/view/36384/binding-site-prediction-in-protein</link>
	<title><![CDATA[Binding Site Prediction in Protein !]]></title>
	<description><![CDATA[<p><span>The interaction between proteins and other molecules is fundamental to all biological functions. In this section we include tools that can assist in prediction of interaction sites on protein surface and tools for predicting the structure of the intermolecular complex formed between two or more molecules (docking).</span></p><h4>Pockets Identification</h4><p><a href="http://sts.bioengr.uic.edu/castp/" target="_blank">CASTp</a></p><div style="text-align: justify;">Automatic Identification of pockets and cavities in proteins structure, and quantitation of their volumes using Delaunay triangulation. Available also as PyMOL plugin</div><p><a href="http://www.bioinformatics.leeds.ac.uk/pocketfinder/" target="_blank">Pocket-Finder</a></p><div style="text-align: justify;">Automatic identification of pockets and cavities in proteins structure, and quantitation of their volumes.</div><p><a href="http://gecco.org.chemie.uni-frankfurt.de/pocketpicker/index.html" target="_blank">PocketPicker</a></p><div style="text-align: justify;">Grid-based technique for the analysis of protein pockets. PocketPicker available as a plugin for&nbsp;<a href="https://bip.weizmann.ac.il/toolbox/structure/pymol.htm">PyMOL</a></div><div style="text-align: justify;">&nbsp;</div><div style="text-align: justify;"><h4>Binding Site Prediction</h4>
<p><a href="http://consurf.tau.ac.il/" target="_blank">ConSurf</a></p>
</div><div style="text-align: justify;">&nbsp;</div><div style="text-align: justify;">Identification of functional regions in proteins by surface-mapping of phylogenetic information</div><div style="text-align: justify;">&nbsp;</div><div style="text-align: justify;"><a href="http://www-cryst.bioc.cam.ac.uk/~crescendo/crescendo.php" target="_blank">CRESCENDO</a></div><div style="text-align: justify;">&nbsp;</div><div style="text-align: justify;">Identification protein interaction sites. It uses sequence conservation patterns in homologous proteins to distinguish between residues that are conserved due to structural restraints from those due to functional restraints.&nbsp;&nbsp;</div><div style="text-align: justify;">&nbsp;</div><div style="text-align: justify;"><strong>Ligand Binding Sites</strong></div><div style="text-align: justify;">&nbsp;</div><div style="text-align: justify;"><a href="http://www.sbg.bio.ic.ac.uk/~3dligandsite/" target="_blank">3DLigandSite</a></div><div style="text-align: justify;">&nbsp;</div><div style="text-align: justify;">The server utilizes protein-structure prediction to provide structural models of the binding site. Ligands bound to structures are superimposed onto the model and use to predict the binding site.</div><div style="text-align: justify;">&nbsp;</div><div style="text-align: justify;">F<a href="http://cssb.biology.gatech.edu/skolnick/files/FINDSITE/" target="_blank">INDSITE</a></div><div style="text-align: justify;">&nbsp;</div><div style="text-align: justify;">A threading-based method for ligand-binding site prediction and functional annotation based on binding-site similarity across superimposed groups of threading templates.</div><div style="text-align: justify;">&nbsp;</div><div style="text-align: justify;">
<p><a href="http://scoppi.biotec.tu-dresden.de/pocket/" target="_blank">LIGSITE<sup>csc</sup></a></p>
<div style="text-align: justify;">&nbsp;</div><div style="text-align: justify;">Prediction of binding site by pocket identification using the Connolly surface and degree of conservation</div>
<p><a href="http://metapocket.eml.org/" target="_blank"></a></p>
</div><div style="text-align: justify;">&nbsp;</div><div style="text-align: justify;"><a href="http://metapocket.eml.org/" target="_blank">metaPocket</a>A meta server for ligand-binding site prediction. metaPocket use&nbsp;<a href="https://bip.weizmann.ac.il/toolbox/structure/binding.htm#ligsite">LIGSITE<sup>csc</sup></a>,&nbsp;<a href="https://bip.weizmann.ac.il/toolbox/structure/binding.htm#pass">PASS</a>,&nbsp;<a href="https://bip.weizmann.ac.il/toolbox/structure/binding.htm#qsite">Q-SiteFinder</a>&nbsp;and&nbsp;<a href="http://www.biochem.ucl.ac.uk/~roman/surfnet/surfnet.html" target="_blank">SURFNET</a></div>]]></description>
	<dc:creator>Poonam Mahapatra</dc:creator>
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