<![CDATA[BOL: Related items]]> https://bioinformaticsonline.com/related/30140?offset=340 https://bioinformaticsonline.com/blog/view/30874/important-journals-blogs-and-forums-for-bioinformaticians Wed, 08 Feb 2017 09:15:31 -0600 https://bioinformaticsonline.com/blog/view/30874/important-journals-blogs-and-forums-for-bioinformaticians <![CDATA[Important Journals, Blogs and Forums for Bioinformaticians]]> Journals. Most journals have RSS feeds for their current updates.

Blogs. Some of these blogs are very relevant to bioinfo jobs. Others are more personal interest.

  • Variance Explained: David Robinson’s blog (Data Scientist at Stack Overflow, works in R and Python).
  • Global Biodefense: News on pathogens, outbreaks, and preparedness, with periodic posts on genomics and bioinformatics-related developments and funding opportunities.
  • In between lines of code: Lex Nederbragt’s blog on biology, sequencing, bioinformatics, …
  • Simply Statistics: A statistics blog by Rafa Irizarry, Roger Peng, and Jeff Leek.
  • Bits of DNA: Reviews and commentary on computational biology by Lior Pachter (fair warning: dialogue here can get a bit heated!).
  • Blue Collar Bioinformatics: articles related tool validation and the open source bioinformatics community.
  • Microbiome Digest – Bik’s Picks: A daily digest of scientific microbiome papers, by Elisabeth Bik, Science Editor at uBiome.
  • Living in an Ivory Basement: Titus Brown’s blog on metagenomics, open science, testing, reproducibility, and programming.
  • Enseqlopedia: James Hadfield’s blog on all things NGS.
  • Epistasis Blog: Jason Moore’s computational biology blog.
  • RStudio Blog: announcements about new RStudio functionality, updates about the tidyverse, and more.
  • nextgenseek.com: Next-Gen Sequencing Blog covering new developments in NGS data & analysis.
  • RNA-Seq Blog: Transcriptome Research & Industry News.
  • The Allium: We all need a little humor in our lives. Like The Onion, but for science.

Forums.

]]> Jit https://bioinformaticsonline.com/bookmarks/view/36861/eagler-a-scaffolding-tool-for-long-reads Mon, 04 Jun 2018 05:26:03 -0500 https://bioinformaticsonline.com/bookmarks/view/36861/eagler-a-scaffolding-tool-for-long-reads <![CDATA[EAGLER: a scaffolding tool for long reads.]]> EAGLER is a scaffolding tool for long reads. The scaffolder takes as input a draft genome created by any NGS assembler and a set of long reads. The long reads are used to extend the contigs present in the NGS draft and possibly join overlapping contigs. EAGLER supports both PacBio and Oxford Nanopore reads.

The tool should be compatible with most UNIX flavors and has been successfully tested on the following operating systems:

  • Mac OS X 10.11.1
  • Mac OS X 10.10.3
  • Ubuntu 14.04 LTS
https://bib.irb.hr/datoteka/844447.Diplomski_2015_Luka_terbi.pdf

Address of the bookmark: https://github.com/mculinovic/EAGLER

]]> Jit https://bioinformaticsonline.com/opportunity/view/30928/jrf-bioinformatics-job-vacancies-in-tezpur-university Tue, 14 Feb 2017 16:40:26 -0600 <![CDATA[JRF Bioinformatics job vacancies in Tezpur University]]> Memo No. DoRD/CSE/SSS/20-295/112-A Date: 01/02/2017

Project Title : “Integrating genome scale metabolic analysis of model plant pathogen Ralstonia solanacearum with RNAseq and fluxomics”

Essential qualification : M.Tech. in CSE/IT (With specialization in Computational Biology/Bioinformatics) or M.Sc. in Bioinformatics/Biosciences/Molecular Biology Biotechnology preferably with NET/GATE/BET. Candidates should have minimum 55 % mark both in 10th and 10+2 Science examinations and mathematics at 10+2 Science. Desirable: Preference will be given to the candidates having experience in computational analysis of genome sequences or similar projects.

No. of Post : 01

Remuneration : Rs. 25,000/- for the 1st two years and Rs. 28,000/- for the 3rd year for SRF and applicable to the candidate having post graduate degree in Basic Science with NET/GATE/BET qualification or post graduate degree in professional course. Rs. 12,000/- for the 1st two years and Rs. 14,000/- for the 3 rd year for SRF,

Age : 28 years

Duration : Three (03) years or till completion of the project or until further order, whichever is earlier.

Hiring Process : Walk - In
Job Role: Research/JRF/SRF

Walk-in-interview will be held on 17th February, 2017, 11.15 a.m. at the office of the Head, Department of Computer Science and Engineering, Tezpur University.

Interested candidates may appear before the interview board with original documents from 10th standard onwards and photocopies of mark sheets, certificates, testimonials, caste certificate (if applicable), experience certificate certificates of NET/GATE/BET or similar examination qualifications, any other testimonials and a copy of recent curriculum vitae (CV) on the day of interview.

More at http://www.tezu.ernet.in/ProjectWalkin/Advt-DoRD-CSE-SSS-20-295-112-A.pdf

]]> https://bioinformaticsonline.com/bookmarks/view/36985/swalo-scaffolding-with-assembly-likelihood-optimization Wed, 20 Jun 2018 02:45:16 -0500 https://bioinformaticsonline.com/bookmarks/view/36985/swalo-scaffolding-with-assembly-likelihood-optimization <![CDATA[SWALO: Scaffolding with assembly likelihood optimization]]> Address of the bookmark: https://atifrahman.github.io/SWALO/

]]> Jit https://bioinformaticsonline.com/blog/view/37396/converting-a-vcf-into-a-fasta-given-some-reference Fri, 20 Jul 2018 10:03:53 -0500 https://bioinformaticsonline.com/blog/view/37396/converting-a-vcf-into-a-fasta-given-some-reference <![CDATA[Converting a VCF into a FASTA given some reference !]]> Samtools/BCFtools (Heng Li) provides a Perl script vcfutils.pl which does this, the function vcf2fq (lines 469-528)

This script has been modified by others to convert InDels as well, e.g. this by David Eccles

./vcf2fq.pl -f <input.fasta> <all-site.vcf> > <output.fastq>

https://github.com/gringer/bioinfscripts/blob/master/vcf2fq.pl

https://github.com/lh3/samtools/blob/master/bcftools/vcfutils.pl

]]> Jit https://bioinformaticsonline.com/opportunity/view/32227/postdoctoral-research-position-in-bioinformatics-in-milan Thu, 20 Apr 2017 12:53:12 -0500 <![CDATA[Postdoctoral Research Position in Bioinformatics in Milan]]> The lab of Immunobiology of Neurological Disorders has a main interest in the biological processes associated with multiple sclerosis, an inflammatory disorder of the central nervous system. The projects of interest for this application involve research on translational bioinformatics in complex human neurological disorders.

You have a PhD in Computational Science, Bioinformatics, or equivalent, and expertise in analysis and modeling of human RNA-seq data, statistics, data mining and machine learning. Excellent communication skills in English (written and oral) is a must. Flexibility and willingness to work across multiple projects and technologies in a rapidly evolving scientific context is required.
Salary will depend on qualification and experience. Starting date: immediate.

Interested candidates should send to farina.cinthia@hsr.it:

1. CV (please show evidences of relevant titles, projects, courses, references, etc.)
2. One page with a list of research topics (i.e. ongoing projects)
3. earliest availability

4. 2-3 contact names

]]> https://bioinformaticsonline.com/opportunity/view/31258/bioinformatics-walk-in-interview-at-tezpur-university Thu, 02 Mar 2017 04:24:46 -0600 <![CDATA[Bioinformatics walk-in-interview at Tezpur University]]> A walk-in-interview will be held on 09 March, 2017, 11.15 a.m. at the office of the Head, Department of Computer Science and Engineering, Tezpur University for one (01) temporary position of Junior Research Fellow (JRF) in the DBT, Govt. of India sponsored project entitled “Integrating genome scale metabolic analysis of model plant pathogen Ralstonia solanacearum with RNAseq and fluxomics” under Dr. Siddhartha Sankar Satapathy (ssankar@tezu.ernet.in), Associate Professor, Department of Computer Science and Engineering, Tezpur University.

Interested candidates may appear before the interview board with original documents from 10th standard onwards and photocopies of mark sheets, certificates, testimonials, caste certificate (if applicable), experience certificate certificates of NET/GATE/BET or similar examination qualifications, any other testimonials and a copy of recent curriculum vitae (CV) on the day of interview.

Essential qualification: M.Tech. in CSE/IT (With specialization in Computational Biology/Bioinformatics) or M.Sc. in Bioinformatics/Biosciences/Molecular Biology Biotechnology preferably with NET/GATE/BET.

Candidates should have minimum 55 % mark both in 10th and 10+2 Science examinations and mathematics at 10+2 Science.

Desirable: Preference will be given to the candidates having experience in computational analysis of genome sequences or similar projects.

Remuneration: Rs. 25,000/- (Rupees twenty five thousand) only + HRA as admissible per month for the 1st two years and Rs. 28,000/- (Rupees twenty eight thousand) only + HRA as admissible per month for the 3rd year for SRF and applicable to the candidate having post graduate degree in Basic Science with NET/GATE/BET qualification or post graduate degree in professional course. Rs. 12,000/- (Rupees twelve thousand) only + HRA as admissible per month for the 1st two years and Rs. 14,000/- (Rupees fourteen thousand) only + HRA as admissible per month for the 3 rd year for SRF, for the candidate without NET/GATE/BET qualification. HRA will not be provided if campus accommodation is availed.

Age: Candidate shall not be more than 28 years of age on the date of interview. Upper age limit may be relaxed up to 5 years in the case of candidate belonging to SC/ST/ OBC/Women/Differently abled.

Duration: Three (03) years or till completion of the project or until further order, whichever is earlier.

N.B. No TA/DA will be paid to the candidates for attending the interview. For further details please contact: Dr. S. S. Satapathy Associate Professor Department of Computer Science and Engineering Tezpur University, Napaam-784028 Email: ssankar@tezu.ernet.in Contact no.: +91-9435979648

More Info: www.tezu.ernet.in/ProjectWalkin/Advt-DoRD-CSE-SSS-20-295-188-A.pdf

]]> https://bioinformaticsonline.com/bookmarks/view/38210/skesa-strategic-k-mer-extension-for-scrupulous-assemblies Wed, 14 Nov 2018 04:45:41 -0600 https://bioinformaticsonline.com/bookmarks/view/38210/skesa-strategic-k-mer-extension-for-scrupulous-assemblies <![CDATA[SKESA: strategic k-mer extension for scrupulous assemblies]]> SKESA is a DeBruijn graph-based de-novo assembler designed for assembling reads of microbial genomes sequenced using Illumina. Comparison with SPAdes and MegaHit shows that SKESA produces assemblies that have high sequence quality and contiguity, handles low-level contamination in reads, is fast, and produces an identical assembly for the same input when assembled multiple times with the same or different compute resources.

Source code for SKESA is freely available at https://github.com/ncbi/SKESA/releases.

Research Paper @ Link

SKESA algorithm are as follows:

image

Address of the bookmark: https://github.com/ncbi/SKESA/releases

]]> Jit https://bioinformaticsonline.com/bookmarks/view/38526/versatile-genome-assembly-evaluation-with-quast-lg Fri, 21 Dec 2018 22:06:31 -0600 https://bioinformaticsonline.com/bookmarks/view/38526/versatile-genome-assembly-evaluation-with-quast-lg <![CDATA[Versatile genome assembly evaluation with QUAST-LG]]> QUAST-LG is an extension of QUAST intended for evaluating large-scale genome assemblies (up to mammalian-size).

QUAST-LG is included in the QUAST  package starting from version 5.0.0 (download the latest release). Run QUAST as usual and do not forget to add ‐‐large option to your command!

A short list of the new features (see CHANGES for all):

  • Significant speedup achieved by both use of new fast aligner (minimap2) and the refactoring of alignment analyzing modules
  • New k-mer-based completeness and correctness metrics
  • BUSCO added for enhanced reference-free analysis
  • The concept of upper bound assembly (theoretical limits on the assembly completeness and contiguity for a given genome and set of reads)

Address of the bookmark: http://cab.spbu.ru/software/quast-lg/

]]> Jit https://bioinformaticsonline.com/blog/view/38765/list-of-tools-frequently-used-while-genome-assembly Tue, 22 Jan 2019 09:39:02 -0600 https://bioinformaticsonline.com/blog/view/38765/list-of-tools-frequently-used-while-genome-assembly <![CDATA[List of tools frequently used while genome assembly]]> List of tools frequently used while genome assembly:

I have used the following assemblers

I have used the following mappers

I have used the following polishing tools

I have used the following tools to assess genome assembly characteristics

If you have any ideas or superior tools we have missed please let us know in the comments.

]]> BioStar