BOL: Related items

CLgenomics

Radha Agarkar — Fri, 03 Mar 2017 09:57:28 -0600

CLgenomics is a standalone desktop software specifically designed for bacterial genome analysis. This program has a powerful multi-genome browser, which enables rapid and responsive exploration of bacterial genomes.

To use CLgenomics, individual genome data (genome sequences + annotation details) are compiled and saved in a specially formatted file called CLG (ChunLab Genomics). Each CLG file corresponds with one bacterial genome. If multiple genomes are being considered and compared, multiple CLG files are needed. ChunLab offers >40,000 CLG files of publicly available Bacterial and Archaeal genomes.

Address of the bookmark: https://chunlab.wordpress.com/clgenomics-software/

MaxBin: software for binning assembled metagenomic sequences based on an Expectation-Maximization algorithm.

Jit — Mon, 06 Mar 2017 04:03:38 -0600

MaxBin is software for binning assembled metagenomic sequences based on an Expectation-Maximization algorithm. Users can understand the underlying bins (genomes) of the microbes in their metagenomes by simply providing assembled metagenomic sequences and the reads coverage information or sequencing reads. For users' convenience MaxBin will report genome-related statistics, including estimated completeness, GC content and genome size in the binning summary page.

Users can use MEGAN or similar software on MaxBin bins to find the taxonomy of each bin after the binning process is finished.

https://academic.oup.com/bioinformatics/article/32/4/605/1744462/MaxBin-2-0-an-automated-binning-algorithm-to

The most recent version of MaxBin is 2.2, which supports the analysis of coassemblies of multiple samples. It is available at this JBEI downloads sites as well as MaxBin and MaxBin 2.0 sourceforge sites.

Address of the bookmark: http://downloads.jbei.org/data/microbial_communities/MaxBin/MaxBin.html

Genomic Impact

Jitendra Narayan — Wed, 10 Jul 2013 01:33:50 -0500

The ongoing genomic research in USA contributed $31 billion to the U.S. gross national product and helped support 152,000 jobs.

Reference: http://www.unitedformedicalresearch.com/wp-content/uploads/2013/06/The-Impact-of-Genomics-on-the-US-Economy.pdf

SeqMule: Automated human exome/genome variants detection

Abhimanyu Singh — Tue, 07 Mar 2017 10:12:36 -0600

SeqMule takes single-end or paird-end FASTQ or BAM files, generates a script consisting of more than 10 popular alignment, analysis tools and runs the script line by line. Users can change the pipeline or fine-tune the parameters by modifying its configuration file. SeqMule also has some built-in functions, such as pooling consensus calls from various callers, plotting a Venn diagram showing intersection among different callers, and downloading databases. SeqMule can be used for both Mendelian disease study and cancer genome study.

Address of the bookmark: http://seqmule.openbioinformatics.org/en/latest/

HTSlib

Jit — Wed, 15 Mar 2017 11:38:05 -0500

Samtools is a suite of programs for interacting with high-throughput sequencing data. It consists of three separate repositories:

Samtools: Reading/writing/editing/indexing/viewing SAM/BAM/CRAM format
BCFtools: Reading/writing BCF2/VCF/gVCF files and calling/filtering/summarising SNP and short indel sequence variants
HTSlib: A C library for reading/writing high-throughput sequencing data

Samtools and BCFtools both use HTSlib internally, but these source packages contain their own copies of htslib so they can be built independently.

Address of the bookmark: http://www.htslib.org/

gbtools: Interactive Visualization of Metagenome Bins in R

Jit — Sun, 26 Mar 2017 15:41:31 -0500

We have developed gbtools, a software package that allows users to visualize metagenomic assemblies by plotting coverage (sequencing depth) and GC values of contigs, and also to annotate the plots with taxonomic information. Different sets of annotations, including taxonomic assignments from conserved marker genes or SSU rRNA genes, can be imported simultaneously; users can choose which annotations to plot. Bins can be manually defined from plots, or be imported from third-party binning tools and overlaid onto plots, such that results from different methods can be compared side-by-side. gbtools reports summary statistics of bins including marker gene completeness, and allows the user to add or subtract bins with each other.

Tool at https://github.com/kbseah/genome-bin-tools

Address of the bookmark: http://journal.frontiersin.org/article/10.3389/fmicb.2015.01451/full

The Centre for Bioinformatics, Biomarker Discovery and Information-Based Medicine (CIBM)

Sun, 14 Jul 2013 12:31:38 -0500

The Centre for Bioinformatics, Biomarker Discovery and Information-Based Medicine (CIBM) is committed to shortening the process of obtaining novel discoveries to achieve distinctively better outcomes in clinical practice and translational individualised medicine.

Link @ http://www.newcastle.edu.au/research-and-innovation/centre/cibm/about-us

JSON

Abhimanyu Singh — Tue, 04 Apr 2017 08:02:39 -0500

JSON (JavaScript Object Notation) is a lightweight data-interchange format. It is easy for humans to read and write. It is easy for machines to parse and generate. It is based on a subset of the JavaScript Programming Language, Standard ECMA-262 3rd Edition - December 1999. JSON is a text format that is completely language independent but uses conventions that are familiar to programmers of the C-family of languages, including C, C++, C#, Java, JavaScript, Perl, Python, and many others. These properties make JSON an ideal data-interchange language.

JSON is built on two structures:

A collection of name/value pairs. In various languages, this is realized as an object, record, struct, dictionary, hash table, keyed list, or associative array.
An ordered list of values. In most languages, this is realized as an array, vector, list, or sequence.

These are universal data structures. Virtually all modern programming languages support them in one form or another. It makes sense that a data format that is interchangeable with programming languages also be based on these structures.

Address of the bookmark: http://json.org/

BioRuby :Ruby packages for biologist

Jitendra Narayan — Mon, 15 Jul 2013 01:36:28 -0500

BioRuby is a package of Open Source Ruby code, with classes for DNA and protein sequence analysis, alignment, database parsing, and other Bioinformatics tools.
BioRuby project provides an integrated environment in bioinformatics for the Ruby language. This project is supported by University of Tokyo (Human Genome Center), Kyoto University(Bioinformatics Center) and the Open Bio Foundation. The project was supported by Information-technology Promotion Agency (IPA) as an Exploratory Software Project in 2005
RubyForge is a home for open source Ruby projects: RubyForge is a home for open source Ruby projects. BioRuby project was started in late 2000, and is still in progress. Currently, there are over 80 files and 15,000 lines (except comment-only lines) in our source code. This might be equivalent to twice or more lines of other languages because of Ruby's extremely high descriptive power.

Classes for
Multiple alignment (Bio::Alignment),
Gene Ontology(Bio::GO),
PDB (Bio::PDB),
FANTOM database(Bio::FANTOM),
GFF (Bio::GFF) and KEGG
Orthology (Bio::KEGG::KO).

They also added support for many applications such as PSORT, SOSUI, TargetP, TMHMM, GenScan, ClustalW, MAFFT, and KEGG API.

Wiki Links
http://bioruby.open-bio.org/wiki/BioRubyOnRails
http://dev.bioruby.org/en/

BioRuby in Anger
http://dev.bioruby.org/en/?BioRuby+in+Anger

BioRuby RDocs
http://bioruby.org/rdoc/

BioRuby Tutorial Website
http://dev.bioruby.org/en/?Tutorial.rd

Why BioRuby Hub for BioRuby
http://www.linuxjournal.com/article/5915

Social Coding Hub for BioRuby
http://www.linuxjournal.com/article/5915

Bioinformatics on Rails: BioRuby Tutorial
http://bioinforuby.blogspot.com/2008/02/bioruby-tutorial.html

RRA BioRuby
http://raa.ruby-lang.org/project/bioruby/

BioRuby Project Discussion Group
http://portal.open-bio.org/mailman/listinfo/bioruby

BioRuby related Projects: Project tree
http://rubyforge.org/softwaremap/trove_list.php?form_cat=252

Reference
http://www.jsbi.org/journal/GIW03/GIW03P191.pdf

Lifemap

Jit — Mon, 10 Apr 2017 05:42:37 -0500

Lifemap is an interactive tool to explore the WHOLE NCBI TAXONOMY. The concept used in Lifemap is similar to the one used in cartography with tools like Google Maps© or Open Street Maps: exploring is done by zooming and panning.

The current tree contains ALL species present in NCBI taxonomy as of October 18th, 2016: 1,135,169 species including 10,545 Archaea, 418,777 Bacteria and 705,847 Eukaryotes. The Lifemap tree is updated every two weeks.

All the nodes in the tree are clickable. This displays various information and options:

The species name (and the associated common name if there is one)
The rank (kingdom, family, class, species...)
Ability to go to the corresponding node/species on NCBI web site (displayed in a new window)
Possibility to download the corresponding subtree in newick extended format
Possibilty to get the whole lineage from the current node/tip to the root of the tree.

Address of the bookmark: http://lifemap-ncbi.univ-lyon1.fr/